• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.185-192
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• 2007

The different parts of 8 edible and medicinal ferns were extracted with 80% ethanol and the bioactive substances and antioxidant activities were investigated. Total polyphenol content was highest in Cyrtomium fortunei rhizome ($57.32mg{\cdot}g^{-1}$) and followed by C. fortunei root, Pyrrosia lingua aerial part and Onoclea sensibilis var. interrupta aerial part. Total flavonoid contents ranged from 1.95 (Onoclea sensibilis var. interrupta rhizome) to $27.51mg{\cdot}g^{-1}$ (O. sensibilis var. interrupta aerial part). Among the samples tested, C. fortunei root and rhizome were found to be most effective in both DPPH and ABTS radical scavenging assay. Except in Selaginella tamariscina underground part and Osmunda japonica aerial part, most of the tested samples showed strong potential (above 87%) in inhibition rate on linoleic acid oxidation.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.348-354
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• 2006

Homogenized prothallus of 6 species (Cyrtomium falcatum, Cyrtomium caryoptideum var. coreanum, Dryopteris varia, Asplenium incisum, Camptosous sibiricus and Phyllitis scolopendrium) were treated with gamma radiation or by chemical mutagenesis with EMS, NMU, $NaN_3$ and colchicine to assess their sensitivities for each treatments and also with the aim of inducing mutations. Generally, decrease of proliferation ratio was dose-dependent and time-dependent. Based on proliferation ratio, optimum dose of gamma irradiation was $5{\sim}10krad$ except in D. varia with 20krad. Optimum condition of EMS treatment was considered as 50mM for 3h and for NMU as $5{\sim}10mM\;for\;1{\sim}3h$. Optimum condition of $NaN_3$ treatment was considered as $0.5{\sim}1mM\;for\;1{\sim}3h$. For colchicine, there were significant differences between species as to the proliferation ratios of prothallus for each treatment.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.259-264
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• 2006

This experiment was conducted to investigate the effects of medium composition, physical condition and growth regulators on prothallus proliferation in 3 fern species belonging to Aspleniaceae (Asplenium incisum, Camptosorus sibiricus and Phyllitis scolopendrium). In all of the 3 species, the effective medium for prothallus proliferation was Murashige and Skoog's basal medium with 1% sucrose and pH 5.8. The optimum $NH_4^+:NO_3^-$ concentration ratio of protahllus proliferation was 20:40mM (1:2 ratio) in A. incisum and P. scolopendrium and 30:30mM (1:1 ratio) in C. sibiricus. However, agar content of medium did not affect the rate of prothallus proliferation. Among the selected 6 kinds of growth regulators (NAA, IAA, 2,4-D, BAP, kinetin and lip), cytokinins generally has a promotive effect, but auxins showed an inhibitory effect on prothallus growth.

• Korean Journal of Plant Taxonomy
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• v.32 no.4
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• pp.481-489
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• 2002

Unrecorded fern species of Korean flora, Thelypteris interrupta (Willd.) K. Iwats. (Thelypteridaceae) and Dryopteris subexaltata (H. Christ) C. Chr. (Dryopteridaceae), were collected from Jeju island off the south coast of Korean peninsula and reported here. T. interrupta is most similar to T. acuminata (Houtt.) C. V. Morton in morphology, however, the two species are readily distinguishable by the plant color including rhizome, stipe base and spore, and the presence of glandular hair as well as the distribution pattern of sorus on lower leaf surface. D. subexaltata is also well distinguishable from its relatives, D. sparsa (D. Don) Kuntze, D. sabaei (Franch. & Sav.) C. Chr., and D. yakusilvicola Sa. Kurata by the presence of proliferation, shape of pinnae, distribution pattern of sori on lower leaf surface, and shape of indusium.

• Korean Journal of Plant Resources
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• v.32 no.5
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• pp.559-564
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• 2019

Cyrtomium yamamotoi Tagawa is an unrecorded species of perennial Pteridophyta that belongs to the genus Cyrtomium of Dryopteriaceae. It is known as native to Taiwan, China and Japan, but we found it in Jeju-si, Jeju-do and Haenam-gun, Jeollanam-do, Republic of Korea in 2018. This species is very similar to C. fortunei J.Sm. having characters such as 1 pinnate compound leaves, globular indusium, but distinguished from the latter by its lanceolate or lanceolate-ovate shaped lateral pinnates of less than 12 pairs, bicolor indusium with dark brown or blackish in center, and margin incised teeth. Here, we provide its description, photographs and a key of related taxa in Korea.

• Korean Journal of Plant Resources
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• v.26 no.4
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• pp.411-416
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• 2013

This study was conducted not only to analyze ${\alpha}$-glucosidase inhibition activity with fronds and rhizomes of nine Pteridophyte species, but also to select the plant materials suitable for natural ${\alpha}$-glucosidase inhibitor. Harvested rhizomes and fronds were washed, freeze-dried and grinded. After conducting ultrasonification extraction for 30 minutes in ultrasonic water tank with 100% methanol solvent, and vacuum filtration, ${\alpha}$-glucosidase inhibition activity was measured. Acarbose was used as the positive control. After mixing $100{\mu}L$ of 0.7 unit ${\alpha}$-glucosidase enzyme solution into $50{\mu}L$ of extract and reacting them at $37^{\circ}C$ for 10 minutes, $50{\mu}L$ of 1.5 mM ${\rho}$-NPG solution was taken and reacted at $37^{\circ}C$ for 20 minutes. The reaction was stopped with 1 mL of 1 M $Na_2CO_3$ and absorbance was measured in 405 nm. With the regression analysis, the content of solubility solids (the value of $IC_{50}$) which can inhibit 50% of 0.7 unit ${\alpha}$-glucosidase solution's activity was investigated. The frond ($IC_{50}=14.00{\sim}913.33{\mu}g{\cdot}mL^{-1}$) and rhizome extracts ($IC_{50}=12.93{\sim}205.84{\mu}g{\cdot}mL^{-1}$) of nine Pteridophyte species showed higher ${\alpha}$-glucosidase inhibition activity in comparison with acarbose ($IC_{50}=1413.70{\mu}g{\cdot}mL^{-1}$). The extracts of fronds and rhizomes showed higher value than acarbose by 1.55~100.98 and 6.87~109.33 times each. Especially, ${\alpha}$-glucosidase inhibition activities of Pyrrosia lingua in fronds and Osmunda cinnamomea var. fokiensis in rhizomes were the highest. The necessary biomass of fronds and rhizomes for inhibiting 50% of ${\alpha}$-glucosidase activity showed the lowest value, 0.35, 0.27 mg each, in O. cinnamomea var. fokiensis. $IC_{50}$ value of P. lingua was the highest among fronds of nine Pteridophyte species, but content of soluble solids was 2.4 times less than O. cinnamomea var. fokiensis. So frond of O. cinnamomea var. fokiensis is more economic in comparison with P. lingua. As the result of this study, O. cinnamomea var. fokiensis showed high ${\alpha}$-glucosidase inhibition activity even with small biomass. Therefore it was considered to be high-valued economic material as natural ${\alpha}$-glucosidase inhibitor.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.04a
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• pp.38-40
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• 2001

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.04a
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• pp.41-42
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• 2001

• Journal of Environmental Impact Assessment
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• v.23 no.4
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• pp.251-270
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• 2014

The purpose of this study is to offer the basic data and method for assessing the naturaliness of temple environment by applying the ecological index of plant to quantitatively and objectively assess the flora and to understand the flora distributed in major temple forest. The ecological index of plant was based in the surveyed flora. The whole flora were summarized as 535 taxa including 103 families, 310 genera, 426 species, 5 subspecies, 82 varieties and 22 forms. And, the numbers of taxa in the Beopheungsa were 267 taxa, 314 taxa of the Daeseungsa and 296 taxa of the Seongjusa. The rare plants were 9 taxa including Aristolochia contorta, Viola albida, Arisaema heterophyllumand so forth, and the Korean endemic plants were 11 taxa including Clematis trichotoma, Stewartia pseudocamellia, Vicia chosenensis and so forth. The specific plants by floristic region were 51 taxa including Wistaria floribunda, Celtis aurantiaca, Angelica gigas and so forth, and the pteridophyta were 26 taxa including Osmunda japonica, Polystichum braunii, Onoclea orientalis and so forth. The naturalized plants were 35 taxa including Fallopia dumetorum, Conyza canadensis, Xanthium strumarium and so forth, the invasive alien plants were 3 taxa including Ambrosia artemisiifolia, Aster pilosus and Eupatorium rugosum. In the results of analysing the ecological index of plant in whole temple forests, RI(Rare Index) was 1.7%, 2.1% of EI(Endemic Index), 9.5% of SI(Specific Index), 1.2 of PI(Pteridophyta Index) and 6.5% of NI(Naturalized Index) in narrow sense. In broad sense, FI(Flora Index) was 11.0%, 1.5% of RI, 3.4% of EI, 4.8% of SI, 10.1% of PI, 10.9% of UI(Urbanized Index) and 25.0% of DI(Disturbed Index). The FI of the Daeseungsa was the highest among the three temple forests. In narrow sense, the RI, SI, NI and DI of the Beopheungsa was high, the seongjusa was higher the EI and SI. In broad sense, the RI and SI of the Beopheungsa, the EI, SI and PI of the Seongjusa, the UI is high in the Daeseungsa. In future, if we will analyze ecological index of plant in other temple forests, we will offer the help to establishing the plan of conservation and restoration on ecosystem in whole temple forests.Environmental Specimen Banks (ESBs) are playing pivotal role in monitoring the effect of environmental pollution on the ecosystem based on the retrospective analysis of the representative samples collected regularly and stored in cryogenic condition.

• Korean Journal of Plant Resources
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• v.33 no.5
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• pp.501-515
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• 2020

This study is attempted to prepare criteria to determine target species for conservation, through analyzing vascular flora and medicinal resources plants of Mt. Daedeok and Geumdae-bong in Gangwon Province, Korea. Flora Surveys were conducted 14 times from April, 2017 to October, 2019. Total 428 taxa with 83 families, 245 genera, 356 species, 5 subspecies, 46 varieties and 21 forms were distributed in Mt. Daedeok and Geumdae-bong. Korean endemic species were composed of 19 species. Total 36 taxa of Korean rare plants species were identified including 5 taxa in the degree of CR, 2 taxa in the degree of EN, 12 taxa in the degree of VU, 14 taxa in the degree of LC and 3 taxa in the degree of DD. The naturalized plants were 5 families, 10 genera, 10 species, totaling 10 taxa and medicinal resources plants were 239 taxa with 73 families, 185 genera, 239 species, 30 varieties 4 forms, 4 subspecies. The Urbanization Index (UI) was 2.5% and the Naturalized Index (NI) was 1.9%.