• Journal of Life Science
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• v.13 no.6
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• pp.843-848
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• 2003

Diarrllea and death of chicken have been brought about by fowl typhoid caused by Salmonella gallinarum, which causes a great loss of chicken farms. For the development of the probiotic which can control a fowl typhoid of S. gallinarum without any adverse effect of commercial existing antibiotics, we isolated antagonistic intestinal bacteria against S. gallinarum from a bowel of the chicken which was pastured in a chicken farm of Gumi, Kyoungbuk. An Y3 strain which had a strong antagonistic ability to S. gallinarum was selected as a candidate of chicken probiotic microorganism among isolated strains. It was identified as a Bacillus amyloliuefaciens by 98% similarity by the result of cultural, physiological, biochemical test and Biolog system$(Microlog^{TM} 4.0)$, and named as Bacillus amyloliquefaciens Y3. The strain showed the strongest antagonistic activity and a good growth at pH 5-9, $37^{\circ}C.$

• Korean Journal of Microbiology
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• v.39 no.3
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• pp.161-165
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• 2003

Ornithobacterium rhinotracheale (OR) is a recently described gram-negative rod-shaped bacterium associated with respiratory tract infection in poultry. In order to investigate current occurrence of OR infection and to evaluate antibiotic susceptibility, the prevalence of OR antibody in domestic chickens were examined and the minimal inhibitory concentrations(MICs) of 8 antibiotics for 11 OR isolates was determined. All isolates tested were mostly susceptible to three antibiotics, ampicillin (MICs ranging from 0.38 ${\mu}g$/ml to 2 ${\mu}g$/ml), tetracycline (MICs 0.094～3 ${\mu}g$/ml) and doxycycline (MICs 0.047～4 ${\mu}g$/ml) but resistant to genatmicin. Ciprofloxacin, norfloxacin, enrofloxacin, and ofloxacin gave most isolates inhibition only in case of a higher concentration (MICs ranged in most cases from 3 ${\mu}g$/ml to 48 ${\mu}g$/ml). Out of 188 chicken flocks including broilers, broiler breeders, and layers, seropositive flock to OR were detected in 5 broilers (4%), 17 broiler breeders (50%), and 16 layers (55.2%), using commercial OR enzyme-linked immunosorbent assay(ELISA) kits. It suggested that OR infection was widespreaded in poultry farms in Korea.

• KOREAN POULTRY JOURNAL
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• v.5 no.10 s.48
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• pp.57-72
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• 1973

1. 육추율, 육성율, 성계생존율 육추율은 99.9$\%$로서 1회 98.5$\%$, 2회 99.2$\%$, 3회 99.8$\%$, 4회 98.3$\%$, 5회 98.7$\%$, 6회 99.7$\%$와 비슷하였다. 육성율은 평균 98.8$\%$로서 1회 97.5$\%$, 2회 97.2$\%$, 3회 96.6$\%$, 4회 97.9$\%$와는 큰차가 없었고 5회 89.7$\%$ 6회 87.1$\%$ 보다는 약 12$\%$가 높게 나타났다. 이는 MD 백신접종 때문인 것으로 생각되며 성계생존율은 평균 86.3$\%$로서 87.3$\%$ 보다는 약 1$\%$낮았고 1회 80.3$\%$ 2회 84.8$\%$보다는 약 4.6$\%$ 높고 3회 71.8$\%$, 5회 71.4$\%$, 6회 75.3$\%$에 비하면 19$\%$나 높았다. 2. 성성숙일령 전체평균은 157.3일로 가장 빠른 구는 4구의 146일이고 가장 늦은 구는 10구와 17구의 161일이였다. 3. 산란율, 산란지수 산란율은 평균 62.0$\%$이고 가장 높은 구는 15구의 68.9$\%$ 이였다. 산란지수는 평균 205.1개로 1회 190.8, 2회 198.3, 3회 184.2, 5회 186.5, 6회 189.7개에 비하여 높고 4회때와 근사하였다. 4. 사료요구율 전체평균 3.23으로 1회 3.54, 2회 3.1과는 근사하나 3회 2.98, 4회 2.87 5회 2.83에 비하여 낮은 것은 사양표준은 작년도와 같으나 원료사료 특히 단백질 사료의 품질저하에 원인이 있었다. 5. 난평균 중량 검정계군 전체의 평균난중은 61.2g으로 최상위구는 64.70g, 최하위구는 58.3g이였다. 6. 체중 검정계의 전체의 평균제중은 300일령 1925.1g, 500일 1938.4g이며 유색품종은 300일령 1986.7g, 500일령 1948.4g이였다. 7. 사료섭취량 사료섭취량 평균은 1일수당 육추기 32.1g 육성기 73.0g 산란기 115.6g이었다. 8. 경제성 총 수입은 7,269,072.34으로 전체수입의 85$\%$가 계란수입이고 15$\%$는 폐계수입이었다. 지출은 5049,690으로 전체 지출의 99.95$\%$가 사료비 0.05$\%$가 초생추대이었다. 9. 후기 검정수수미달은 참고구로 처리하였고 축산시험장과 대천종축장에서는 당초부터 참고구로 출품하였다. 대신의 개리슨, 신기의 하바드 코메트는 수정율이 낮아서 검정수수가 미달되었으며 영국의 Sykes International Co. 에서 출품한 Sykes Tinted(WL$\times$RIR BX)는 출품회사에서 직접 종란을 항공편에 보내 인수 부화하여 검정하였다. 7회 부터는 초생추에 MD(뎁타백) 백신을 하여 초산전후에 폐사율이 적었으며 72년 11월부터 국산어분에 의한 염도의 과다에 의하여 약 2주일 설사를 하여 상당히 스트레스를 받은 바 있었다.

• Korean Journal of Clinical Laboratory Science
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• v.47 no.2
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• pp.71-77
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• 2015

Antimicrobial resistant bacteria has recently emerged and been disseminated in livestock environments because of excessive use of antimicrobial agents for the therapeutic and growth promotion purposes to food animals. In particular, there is potential for multidrug-resistant bacteria that can be transmitted from animals to mankind. In this study, we investigated the genotypes of E. coli strains isolated from humans and chickens using multi-locus sequence typing (MLST) and antimicrobial resistance patterns by disk diffusion method along with integron study involving antimicrobial resistance mechanisms. From July 2013 to July 2014, E. coli strains isolated from clinical specimens (n=44) and poultry chickens (n=34). ST131 (n=20) was most common in human-derived E. coli. ST752 (n=7) was most common in chicken-derived E. coli, with four isolates each for ST117, ST189, and ST69. Of the 44 E. coli strains isolated from humans, 25 of had a class 1 integron, as opposed to only 11 of 34 strains in the E. coli isolated from chickens. There were differences in genotypes and antimicrobial resistance patterns between the chicken-derived and the human-derived E. coli.

• Korean Journal of Agricultural and Forest Meteorology
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• v.22 no.4
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• pp.299-311
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• 2020

This paper analyzes the distribution of water vapor pressure and relative humidity in complex terrains by collecting weather observation data at 6 locations in the valley in Jungdae-ri, Ganjeon-myeon, Gurye-gun, Jeolla South Province and 14 locations in Akyang-myeon, Hadong-gun, Gyeongsang South Province, which form a single drainage basin in rural and mountainous regions. Previously estimated water vapor pressure used in the early warning system for agrometeorological hazard and actual water vapor pressure arrived at using the temperature and humidity that were measured at the highest density (1.5 m above ground) at every hour in the valley of Jungdae-ri between 19 December 2014 and 23 November 2015 and in the valley of Akyang between 15 August 2012 and 18 August 2013 were compared. The altitude-specific gradient of the observed water vapor pressure varied with different hours of the day and the difference in water vapor pressure between high and low altitudes increased in the night. The hourly variations in the water vapor pressure in the weather stations of the valley of Akyang with various topographic and ground conditions were caused by factors other than altitude. From the observed data of the study area, a coefficient that adj usts the variation in the water vapor pressure according to the specific difference in altitude and estimates it closer to the actual measured level was derived. Relative humidity was simulated as water vapor pressure estimated against the saturated water vapor pressure, thus, confirming that errors were further reduced using the derived coefficient than with the previous method that was used in the early warning system.

• Korean Journal of Poultry Science
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• v.47 no.4
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• pp.199-210
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• 2020

Footpad dermatitis (plantar surface footpad lesions) is an increasing problem in the global poultry industry, affecting foot quality, overall welfare, and production performance. The growing consumer demand for chicken feet in Asian markets has given poultry companies interest in reducing footpad dermatitis. The lesions are multiply associated with various factors affecting the moisture content of litter such as nutrition, drinker type and management, environmental conditions (e.g., temperature, relative humidity, ammonia levels, ventilation rate), and flock health. This review addresses footpad dermatitis characteristics, causes, and the assessment system to provide a guide for future research.

• Journal of Ginseng Research
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• v.24 no.2
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• pp.68-73
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• 2000

To elucidate the effect of chitin and chitosan on the production of ginsenosides and growth, ginseng hairy root was cultured on the 1/2 MS medium supplemented with chitin and chitosan of various concentrations and culture period. The highest growth was obtained with 1 mg/L of chitin. However, the growth was inhibited by 20 mg/L or above. The contents and productivity of ginsenosides were the highest when ginseng hairy roots were cultured on 40 mg/L chitin and applied of the third-weeks of culture period. Ginseng hairy root culture with 1 mg/L of chitosan resulted in the best growth, but the highest ginsenosides level was appeared in 30 mg/L chitosan. Ginsenosides content was increased when it was treated at the forth-week after culture as 30 mg/L of chitosan.

• Journal of Acupuncture Research
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• v.25 no.6
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• pp.125-133
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• 2008

Objectives : The aim of this study is to review $\ll$Chimgudaesung$\gg$ on documentary characteristics such as truth or falsehood of authors, editions, the relationship of $\ll$Hyeongibiyo(玄機秘要)$\gg$. Materials and Methods : Based on Hwangyongsang(黃龍祥)'s study, the author of $\ll$Chimgudaesung$\gg$, the number of volumes and edition, basic contents and constitution, characteristic of reference, and the documentary research results will be arranged. Results and Conclusions : 1. $\ll$Chimgudaesung$\gg$ was made by Geunhyeon based on Yanggyeju(楊繼洲)'s $\ll$Hyeongibiyo$\gg$, and in the 29th year of the Manryeok(萬曆) era Jomunbyeong(趙文炳) saw to the inscription. Therefore, the author must be recorded as 'Originally by Yanggyeju of the Myeong(明) dynasty, revised by Geunhyeon'. 2. The existing Myeong dynasty editions are mostly Leewolgyu(李月桂)'s 'Jungsu edition(重修本)' from the 14th year of the Sunchi(順治) era of the Qing(淸) dynasty and Wangbo(王輔)'s 'Chesu edition (遞修本)' Leewolgyu and Wangbo's preface was deleted and the original text was supplemented from the 37th year of the Ganghui(康熙) era. There are many traces of revision, supplementation and copying by people of later generations in these editions. 3. The 'Chukin edition(縮印本)' of $\ll$Chimgudaesung$\gg$ is mostly a merge of the Myeong dynasty editions and used much of the new revised 'Chesu edition'. This editions should not ever be used again as an original in putting the $\ll$Chimgudaesung$\gg$ in order. 4. After $\ll$Chimgudaesung$\gg$ was published Jomunbyeong had a craftsman publish 4 drawings of $\ll$Donginmyeongdangdo(銅人明堂圖)$\gg$, of which the originally published ones were w drawings of the front and rear, and when Jomunbyeong republished he added 2 Cheukindo(側人圖) of the frontal side(正側) and rear side(背側). 5. When Geunhyeon edited $\ll$Chimgudaesung$\gg$ only 14 medical books were used and quotes copied from other texts were always reedited. Most of the origins of the collected text are shown under the index, but many do not match with the original text. Also many documents were copied from medical books from later times and not the primal text. 6. The annotated sections of $\ll$Chimgudaesung$\gg$ such as 'Yangsi(楊氏)', 'Yangsijip(楊氏集)', 'Yangsijuhae(楊氏注解)', 'Hyeongibiyo' are all from Yanggyeju's $\ll$Hyeongibiyo$\gg$. Of these the origins for the sections marked 'Yangsijip' can be found, but some of the origins for sections annotated 'Yangsi' cannot be found.

• Journal of Plant Biotechnology
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• v.31 no.3
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• pp.239-248
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• 2004

This experiment was carried out to confirm that phytochrome regulates anthocyanin bio-synthesis during cell suspension culture system of grape or not. In suspension culture of grape, maximum accumulation of anthocyanin was observed at the stationary phase under continuous white light condition. From mono-chromatic light interruption for 24h at the 4th or 7th day on the suspension cultured cells, the anthocyanin accumulation was highly enhanced at the light interruption at 7th day than 4th day under all monochromatic light treatment. However, the cell growth patterns were not affected by any light treatment. In the darkness, the anthocyanin synthesis was very low but remarkably increased by blue light or red light irradiation. However, the increase of anthocyanin accumulation by blue or red light was suppressed by far-red light in the suspension cells of grape. This suppression by far-red light on the anthocyanin synthesis also observed on the cells treated red or far-red light alternatively. These results implied that phytochrome regulation system may be involved in the anthocyanin biosynthesis of the suspension grape cells. By RNA expression analysis, chalcone synthase (CHS) gene was expressed highly by blue and red light but low by far-red light. The synergistic increase of CHS gene expression was also observed at the treatment of blue light followed by red for 24h. This result may explain the increase of anthocyanin accumulation in B/R treatment. Although the expression of phytochrome gene (PHYA or PHYB) was not highly increased by all light treatment (blue, red, and far-red light) the expression of both PHYA gene and PHYB gene was increased a little in cells treated red or far-red light. In grape suspension cells, the red light enhanced the anthocyanin synthesis, whereas the far-red light was suppressed. Although it was not confirmed whether or not phytochrome gene is activated in anthocyanin accumulating grape cells, we believed that anthocyanin biosynthesis in grape cells may be regulated under phytochrome signal transduction system.

• Korean Journal of Plant Tissue Culture
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• v.28 no.3
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• pp.123-128
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• 2001

In order to develop the biotechnological methods for the mass production of anticancer compounds from tissue culture of Panax ginseng C.A. Mayer, these studies were carried out for the selection of ginseng cell lines containing higher concentration of polyacetylene compounds and optimal condition for their biosynthesis. Panaxynol, one of ginseng polyacetylene, was not detected in any callus induced from ginseng superior cell lines cultured on MS medium supplemented with $\beta$-chlorophenoxy acetic acid (CPA). Panaxydol, another one of polyacetylene and anticancer compounds, were detected in calli of 5 cell lines by thin layer chromatogram and gas chromatogram. Among the 18 ginseng superior lines, the cell line 30201 has higher content of panaxydol. Especially, panaxydol was not detected in the callus induced from cell line 10301 which cultured on the medium containing CPA only, however, it was detected on the same callus cultured on mixed medium containing CAP 2 mg/L and BA 0.05 mg/L. SH medium was better than MS medium for ginseng callus growth and biosynthesis of polyacetylene, and also found that it was not effected by NAA and sucrose concentration in the culture medium.