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Analysis of Reentry Test for the Donors Showing Reactivity or Grey Zone in a HBV Surface Antigen Assay by a Chemiluminescent Immunoassay (화학발광면역법에 의한 HBV 항원선별검사에서 양성 및 Grey Zone 결과를 보인 헌혈자의 헌혈 보류 해제 검사 결과 분석)

  • Shin, Sunmi;Kang, Jungwon;Lee, Kyeong Rak;Shin, Geon Sik;Kang, Jae-won;Seo, Young Ik;Min, Hyukki
    • The Korean Journal of Blood Transfusion
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    • v.29 no.3
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    • pp.301-309
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    • 2018
  • Background: If donors who were deferred due to the reactivity or grey zone in HBV surface antigen (HBsAg) assay want to donate blood again, they need to pass reentry tests. On the other hand, approximately half of the donors who are subject to the reentry tests cannot be reentered. This study examined the association between the sample to cutoff (S/Co) value of the HBsAg assay and the final results of the reentry test. Methods: This study analyzed the S/Co values of the HBsAg assay and the final results of the reentry tests for the 3,947 donors from January 2008 to December 2017 using the database of Blood Information Management System of the Korean Red Cross. Results: 1,767 donors (44.8%) were not reentered among 3,947 deferred donors. Among 1,585 donors showing ${\geq}10$ of the S/Co value in the HBsAg screening test, 1,542 donors (97.3%) were not reentered. The additional reentry tests were performed on 120 donors who were not reentered in the first reentry test; 98 donors (81.7%) were still not reentered. Overall, 4.6% of the donors showing a grey zone in the HBsAg assay were not reentered. Conclusion: The reentry test needs to be restricted for the deferred donors showing a more than 10 S/Co value. The application of the grey zone of current HBsAg assay will need to be continued to enhance the HBV-related blood safety.

Marker Assisted Selection of Brown Planthopper Resistance and Development of Multi-Resistance to Insect and Diseases in Rice (Oryza sativa L.) (DNA 마커를 이용한 벼멸구 저항성 선발 및 복합내병충성 벼 계통 육성)

  • Lee, Jong-Hee;Yeo, Un-Sang;Cho, Jun-Hyun;Lee, Ji-Yoon;Song, You-Chun;Shin, Mun-Sik;Kang, Hang-Won;Sohn, Jae-Keun
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.413-421
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    • 2011
  • The main objective of this study was to develop the multi-resistance lines to insects(brown planthopper; BPH, rice green leafhopper; GRH) and disease(blast; BL, bacterial blight; BB and rice stripe virus disease;RSV) with good grain quality and plant type by combining conventional breeding and marker assisted selection(MAS) and to eliminate the linkage drag effects between Bph1 gene and culm length, we conducted MAS of Bph1 gene in advanced backcross and double cross progenies. 'Nampyeong', 'Junam' and 'Milyang220' were used as the parent in this study. 'Milyang220' was used as the donor of brown planthopper resistance gene Bph1 with tall culm length. Two backcross progenies were developed using two recipients 'Nampyeong' carrying GRH resistance gene Grh3(t) with good grain appearance and 'Junam' harboring bacterial blight resistance gene Xa3 with short culm length. Two $BC_1$ generations were resulted from the backcrossing of the $F_1$ plants with recurrent parents 'Nampyeong' and 'Junam'. The second rounds of backcrossing($BC_2$) were derived from the cross of selected resistant $BC_1F_1$ plants based on heterozygous genotype of RM28493 linked to Bph1 gene. The double crossed population was constructed from the cross of between each heterozygous $BC_2F_1$ plants at RM28493 locus of '$Nampyeong^*3$ / Milyang220' and '$Junam^*3$ / Milyang220'., The homozygous alleles in Bph1 gene were selected using co-dominant DNA marker RM28493 in double crossed population. Eighty-five lines with multi-resistance to BL, BB, RSV, GRH and BPH were selected by bio-assay and MAS in generation of double crossing. The culm length, head rice ratio and yield of the selected multi resistance lines was ranged from 71 to 88 cm, from 51 to 93%, from 449 to 629 kg/10a. respectively. We can select a promising multi resistance line similar with 'Nampyeong' of major agronomic traits such as culm legnth, head rice ratio and yield. It was designated as Milyang265. Finally this study was developed the multi resistant varieties against to insects and diseases with the good grain quality 'Milyang265' by the advanced backcross and double cross combining MAS and it can be used as genetic resources of multi-resistance to insect and diseases in rice breeding programs.

An Experimental and Numerical Study on the Survivability of a Long Pipe-Type Buoy Structure in Waves (긴 파이프로 이뤄진 세장형 부이 구조물의 파랑 중 생존성에 관한 모형시험 및 수치해석 연구)

  • Kwon, Yong-Ju;Nam, Bo-Woo;Kim, Nam-Woo;Park, In-Bo;Kim, Sea-Moon
    • Journal of Navigation and Port Research
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    • v.42 no.6
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    • pp.427-436
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    • 2018
  • In this study, experimental and numerical analysis were performed on the survivability of a long pipe-type buoy structure in waves. The buoy structure is an articulated tower consisting of an upper structure, buoyancy module, and gravity anchor with long pipes forming the base frame. A series of experiment were performed in the ocean engineering basin of KRISO with the scaled model of 1/ 22 to evaluate the survivability of the buoy structure at West Sea in South Korea. Survival condition was considered as the wave of 50 year return period. Additional experiments were performed to investigate the effects of current and wave period. The factors considered for the evaluation of the buoy's survival were the pitch angle of the structure, anchor reaction force, and the number of submergence of the upper structure. Numerical simulations were carried out with the OrcaFlex, the commercial program for the mooring analysis, with the aim of performing mutual validation with the experimental results. Based on the evaluation, the behavior characteristics of the buoy structure were first examined according to the tidal conditions. The changes were investigated for the pitch angle and anchor reaction force at HAT and LAT conditions, and the results directly compared with those obtained from numerical simulation. Secondly, the response characteristics of the buoy structure were studied depending on the wave period and the presence of current velocity. Third, the number of submergence through video analysis was compared with the simulation results in relation to the submergence of the upper structure. Finally, the simulation results for structural responses which were not directly measured in the experiment were presented, and the structural safety discussed in the survival waves. Through a series of survivability evaluation studies, the behavior characteristics of the buoy structure were examined in survival waves. The vulnerability and utility of the buoy structure were investigated through the sensitivity studies of waves, current, and tides.

A Study on Smart Accuracy Control System based on Augmented Reality and Portable Measurement Device for Shipbuilding (조선소 블록 정도관리를 위한 경량화 측정 장비 및 증강현실 기반의 스마트 정도관리 시스템 개발)

  • Nam, Byeong-Wook;Lee, Kyung-Ho;Lee, Won-Hyuk;Lee, Jae-Duck;Hwang, Ho-Jin
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.32 no.1
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    • pp.65-73
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    • 2019
  • In order to increase the production efficiency of the ship and shorten the production cycle, it is important to evaluate the accuracy of the ship components efficiently during the drying cycle. The accuracy control of the block is important for shortening the ship process, reducing the cost, and improving the accuracy of the ship. Some systems have been developed and used mainly in large shipyards, but in some cases, they are measured and managed using conventional measuring instruments such as tape measure and beam, optical instruments as optical equipment, In order to perform accuracy control, these tools and equipment as well as equipment for recording measurement data and paper drawings for measuring the measurement position are inevitably combined. The measured results are managed by the accuracy control system through manual input or recording device. In this case, the measurement result is influenced by the work environment and the skill level of the worker. Also, in the measurement result management side, there are a human error about the lack of the measurement result creation, the lack of the management sheet management, And costs are lost in terms of efficiency due to consumption. The purpose of this study is to improve the working environment in the existing accuracy management process by using the augmented reality technology to visualize the measurement information on the actual block and to obtain the measurement information And a smart management system based on augmented reality that can effectively manage the accuracy management data through interworking with measurement equipment. We confirmed the applicability of the proposed system to the accuracy control through the prototype implementation.

A Performance Evaluation of the e-Gov Standard Framework on PaaS Cloud Computing Environment: A Geo-based Image Processing Case (PaaS 클라우드 컴퓨팅 환경에서 전자정부 표준프레임워크 성능평가: 공간영상 정보처리 사례)

  • KIM, Kwang-Seob;LEE, Ki-Won
    • Journal of the Korean Association of Geographic Information Studies
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    • v.21 no.4
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    • pp.1-13
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    • 2018
  • Both Platform as a Service (PaaS) as one of the cloud computing service models and the e-government (e-Gov) standard framework from the Ministry of the Interior and Safety (MOIS) provide developers with practical computing environments to build their applications in every web-based services. Web application developers in the geo-spatial information field can utilize and deploy many middleware software or common functions provided by either the cloud-based service or the e-Gov standard framework. However, there are few studies for their applicability and performance in the field of actual geo-spatial information application yet. Therefore, the motivation of this study was to investigate the relevance of these technologies or platform. The applicability of these computing environments and the performance evaluation were performed after a test application deployment of the spatial image processing case service using Web Processing Service (WPS) 2.0 on the e-Gov standard framework. This system was a test service supported by a cloud environment of Cloud Foundry, one of open source PaaS cloud platforms. Using these components, the performance of the test system in two cases of 300 and 500 threads was assessed through a comparison test with two kinds of service: a service case for only the PaaS and that on the e-Gov on the PaaS. The performance measurements were based on the recording of response time with respect to users' requests during 3,600 seconds. According to the experimental results, all the test cases of the e-Gov on PaaS considered showed a greater performance. It is expected that the e-Gov standard framework on the PaaS cloud would be important factors to build the web-based spatial information service, especially in public sectors.

Characteristic study and optimization of culture conditions for Bacillus amyloliquefaciens SRCM 100731 as probiotic resource for companion animal (Bacillus amyloliquefaciens SRCM 100731의 반려 동물용 프로바이오틱스 소재로서의 특성 규명 및 배양 조건 최적화)

  • Ryu, Myeong Seon;Yang, Hee-Jong;Jeong, Su-Ji;Seo, Ji Won;Ha, Gwangsu;Jeong, Seong-Yeop;Jeong, Do-Youn
    • Korean Journal of Microbiology
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    • v.54 no.4
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    • pp.384-397
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    • 2018
  • The aim of this study is to screen the strains of Bacillus spp. possessing safety, probiotic activity, and so on, which can be utilized as probiotic resource for using the feed and supplement food of companion animal. About 300 isolates were isolated from traditional Korean sauces, four isolates that did not have or produce the six kinds of B. cereus type vomiting and diarrhea toxin genes, ${\beta}$-hemolytic, and three kinds of carcinogenic enzymes were selected. Antibiotic gene retention, cell surface hydrophobicity, antibiotic sensitivity, and glucose utilization were analyzed for four isolates, and finally SRCM 100731 was selected. SRCM 100731 was named as Bacillus amyloliquefaciens SRCM 100731 16S rRNA sequencing analysis, and carried out optimization of cell growth for industrial applications such as pet food and feed. The effects of 14 different components on cell growth were investigated and three significant positive factors, molasses, sodium chloride, and potassium chloride were selected as the main factors based on a Plackett-Burman design. In order to find out optimal concentration on each constituent, we carried out central composite design. The predicted optimized concentrations were 7% molasses, 1.1% sodium chloride, 0.5% potassium chloride. Finally, an overall about 7-fold increase in dry cell weight yield ($12.6625{\pm}0.0658g/L$) was achieved using the optimized medium compared with the non-optimized medium ($1.8273{\pm}0.0214g/L$). This research is expected to be highly utilized in the growing pet industry by establishing optimal cultivation conditions for industrial application as well as screening Bacillus amyloliquefaciens SRCM 100731 as probiotic resource for companion animal.

A comparative study of mosquito population density according to the Sejong City areas and old city and new city (세종특별자치시 전역과 구도심 및 신도심에 따른 모기 밀도 비교 연구)

  • Na, Sumi;Doh, Jiseon;Yang, Young Cheol;Ryu, Sungmin;Yi, Hoonbok
    • Korean Journal of Environmental Biology
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    • v.39 no.3
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    • pp.362-373
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    • 2021
  • This study was conducted to establish mosquito distribution density and habitat in Sejong city for the prevention of mosquito-borne infectious diseases. The overall distribution of mosquitoes in the Sejong City was investigated, and the population density of mosquitoes in the old and new city was analyzed. Mosquito populations were determined using MOSHOLE and Blacklight traps once a week overnight. We also compared the mosquito population density of the old city and the new city, and the daily mosquito population was calculated using the data from the smart mosquito trap(DMS). Of all the study sites, Geumnam-myeon had the highest number of mosquitoes captured, and the dominant species were Armigeres subalbatus and Culex pipienspallens. Mosquito species with the potential for transmitting diseases were mainly found in Yeonseo-myeon (106 individual), and Geumnam-myeon (101). Mosquito collection rates by MOSHOLE trap and Blacklight trap were 58.49% and 41.51%, respectively. We concluded that using CO2 would be the most suitable approach for collecting mosquitoes. The mosquito population density in the old city (92.05±7.04) was approximately twice that of the new city(51.50±4.05). Since Sejong City is divided into old city and new city, it is difficult to spot remarkable effects in a standardized way. For effective quarantine, differentiation of quarantine must be established. This study results provide a basis for Sejong City's integrated mosquito control guidelines, and therefore effective control of which we believe will help control the spread of mosquito-borne diseases and reduce damage from mosquitoes.

A Study on the Directions of Sewol Ferry Tragedy Memorial Park Based on the Analysis on Social Discourse and Recognition Evaluation (도심형 메모리얼파크의 사회적 담론 및 인식분석을 통한 4·16 세월호 참사 추모공원 방향성 제안 연구)

  • Kim, Do-Hun
    • Journal of the Korean Institute of Landscape Architecture
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    • v.48 no.6
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    • pp.25-38
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    • 2020
  • The objective of this study is to propose a direction for creating a memorial park for the 250 students victims of the Sewol ferry disaster. To this end, this study first attempted to understand the matters discussed at various levels to create a memorial park and find a way that the park can be built by gathering opinions from the bereaved families and the victims themselves, as well as local residents, and experts. Workshops, competitions, special lectures, and websites, etc, were analyzed. A social discourse analysis methodology was used for systematic analysis, and the analyzed discourse was categorized into 4 types for assessment, and the functions and roles were subdivided into 15 types. To assess the priorities and the adequacy of the discourse, an analytic hierarchy process (AHP) was used among 30 activists, public servants, and experts. Then, a survey was conducted to analyze the perception of the residents (467 participants including the bereaved families) about the memorial park. Based on the results of the analysis, two directions were set for the memorial park. First, is a memorial park to remember the victims in everyday life. It must be a park with various cultural contents instead of a conventional memorial park that is solemn and grave sharing anguish and sorrow. The memorial park for the Sewol ferry disaster must become a space where visitors can naturally encounter and remember the victims. Second, is a park that serves as a catalyst that brings change and innovation to the community. It must be able to bring change to the community with direct and indirect influence. It must serve as an impetus to bring change and innovation to the community in the mid-to-long-term. Having many visitors may also lead to an economic effect. These visitors may not just stay in the park, but even contribute to revitalizing the local businesses. The purpose of this study is to apply the research findings to guide the International Design Competition scheduled for 2020 and serve to establish guidelines for a continuous park management system.

Design and Management Direction of Smart Park for Smart Green City (스마트 그린시티 구현을 위한 스마트 공원 설계·관리 방향)

  • Kim, Yong-Gook;Song, Yu-Mi;Cho, Sang-kyu
    • Journal of the Korean Institute of Landscape Architecture
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    • v.48 no.6
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    • pp.1-15
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    • 2020
  • The purpose of this study is to propose a direction for designing and managing a smart park for realizing a smart green city and to present measures in the landscape field to foster related industries. The research process is as follows. First, the concept of a smart park was operationally defined through a literature review, and three principles to be considered in the process of creation and management were established. Second, in terms of the three principles, problems and implications for improvement were derived through an analysis of established cases of smart parks in new and pre-existing cities. Third, a pool of designs and management standards for each spatial component of a smart park was prepared through literature and case studies, and then further refined through brainstorming with experts in related fields. Fourth, measures were suggested to the government, local governments, and the landscape field to promote smart park creation and management. The main findings are as follows. First, the concept of a smart park is defined as "a park that contributes to securing the social, economic, and environmental sustainability of cities and local communities by supporting citizens' safe and pleasant use of parks and improving the management and operational efficiency by utilizing the digital, environment, and material technologies." Second, the three principles of smart parks are to improve the intrinsic value of parks, to improve the innovative functions of parks to solve urban problems, and to make the design, construction, and management process smart. Third, improvement implications were derived through the analysis of cases of smart parks creation in new and pre-existing cities. Fourth, the directions for smart park design and management were suggested in five aspects: green area, hydroponic facility area, road and plaza area, landscape facilities area, and park design method. Fifth, as for policy implications for revitalizing the construction and management of smart parks, the development of smart park policy business models by city growth stage, and park type, the promotion of pilot projects, the promotion of smart park projects in connection with the Korean New Deal policy, and smart park policies led by landscape experts were presented.

Mechanism of steel pipe reinforcement grouting based on tunnel field measurement results (터널 현장 계측결과를 통한 강관보강 그라우팅의 거동 메커니즘)

  • Shin, Hyunkang;Jung, Hyuksang;Lee, Yong-joo;Kim, Nag-young;Ko, Sungil
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.23 no.3
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    • pp.133-149
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    • 2021
  • This study aims to report the behavioral mechanism of steel pipe reinforcement grouting, which is being actively used to ensure the stability of the excavation surface during tunnel excavation, based on measurements taken at the actual site. After using a 12 m steel pipe attached with a shape displacement meter and a strain gauge to reinforce the actual tunnel surface, behavioral characteristics were identified by analyzing the measured deformation and stress of the steel pipe. Taking into account that the steel pipes were overlapped every 6 m, the measured data up to 7 m of excavation were used. In addition, the behavioral characteristics of the steel pipe reinforcement according to the difference in strength were also examined by applying steel pipes with different allowable stresses (SGT275 and SGT550). As a result of analyzing the behavior of steel pipes for 7 hours after the first excavation for 1 m and before proceeding with the next excavation, the stress redistribution due to the arching effect caused by the excavation relaxation load was observed. As excavation proceeded by 1 m, the excavated section exhibited the greatest deformation during excavation of 4 to 6 m due to the stress distribution of the three-dimensional relaxation load, and deformation and stress were generated in the steel pipe installed in the ground ahead of the tunnel face. As a result of comparing the behavior of SGT275 steel pipe (yield strength 275 MPa) and SGT550 steel pipe (yield strength 550 MPa), the difference in the amount of deformation was up to 18 times and the stress was up to 12 times; the stronger the steel pipe, the better it was at responding to the relaxation load. In this study, the behavior mechanism of steel pipe reinforcement grouting in response to the arching effect due to the relaxation load was identified based on the measured data during the actual tunnel excavation, and the results were reported.