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Effect of a combination of resistance and aerobic exercise training on angiogenesis-related protein expression in different type of skeletal muscle of aged rats (저항성 운동과 유산소 운동 훈련의 병행이 노화쥐 골격근 유형별 혈관신생 관련 단백질 발현에 미치는 영향)

  • Yeo, Hyo-Seong
    • Journal of the Korean Applied Science and Technology
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    • v.38 no.3
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    • pp.750-761
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    • 2021
  • This study was performed to observe the responses of angiogenesis-related protein expression in skeletal muscle of aged rats by regular resistance exercise training with aerobic exercise. For the purpose of the study, naturally aged SD rats (20-24 months, N=18) were used and divided into control (CON, n=6), resistance exercise (RE, n=6), and resistance + aerobic exercise (RE + AE, n=6) groups. RE group performed 3 sets × 4 exercises each session using a ladder for laboratory animals, and RE +AE group performed 2 sets × 3 times of ladder climbing and additional treadmill running (30 min) each session. After 8 weeks of exercise training, soleus muscle and extensor digitorum longus muscle (EDL) were extracted and used for analysis. Western blot was performed to analyze the expression levels of angiogenesis-related proteins (HIF-1α, VEGF, FLK-1, Ang-1, Ang-2) in skeletal muscle. As a result of the study, the expression of HIF-1α, VEGF, FLK-1, Ang-1, and Ang-2 proteins in soleus muscle (type I muscle) was higher in RE +AE than in CON group, and HIF-1α, VEGF, Ang-1, Ang-2 protein expression of RE group was higher than that of CON group. Furthermore, Ang-2 to Ang-1 ratio of RE + AE group was higher than that of RE group, showing differences by exercise type. In EDL muscle (type II muscle), HIF-1α was increased only by RE group, whereas VEGF and FLK-1 protein expressions were increased in both training types, and no difference was observed between the types of exercise training. In addition, there was no difference in angiopoieitin protein expressions in EDL muscle by exercise training. Therefore, in aging, regular exercise training induces skeletal muscle angiogenic response regardless of exercise type, and in particular, the combination of aerobic exercise with resistance exercise may have an additional positive effect on angiogenesis in type I muscle.

Optimizing In Vitro Propagation of Sophora koreensis Nakai using Statistical Analysis (다양한 통계분석 기법을 이용한 개느삼(Sophora koreensis Nakai)의 기내 증식 최적 조건 구명)

  • Jeong, Ukhan;Lee, Hwa;Park, Sanghee;Cheong, Eun Ju
    • Journal of Korean Society of Forest Science
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    • v.110 no.1
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    • pp.53-63
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    • 2021
  • Sophora koreensis Nakai is an indigenous plant in Koreawith a restricted natural range, part of which is in Gangwon province. The species is known to contain phytochemicals that have beneficial effects on human health, and it is economically important in bioindustry. Because of the limited number of plants in a small range of habitats, the mass-propagation method should be developed for use and conservation. In vitro tissue culture is a reliable method in terms of mass propagation from selected clones of the species. We investigated the optimal conditions of the medium in this process, especially focusing on the concentrations of plant growth regulators(PGRs) in the culture of stem-containing axillary buds. Three statistical methods, i.e., ANOVA, response surface method(RSM), and fuzzy clustering were used to analyze the plant growth, number of shoots induced, and shoot length with various combinations of PGRs. Results from the RSM differed from those of the other two methods; thus, the method was not suitable. ANOVA and fuzzy clustering showed similar results. However, more accurate results were obtained using fuzzy clustering because it provided a probability for each treatment. On the basis of the fuzzy clustering analysis, stem tissue produced the greatest number of shoots(11.03 per explant; 63.33%) on a medium supplemented with 5-��M 6-benzylaminopurine and 2.5-��M thidiazuron(TDZ). Proliferation of shoots(2.18 ± 0.21 cm, 63.33%) was attained on a medium supplemented with 2.5-��M BA, 2.5-��M TDZ, and 2.5-��M gibberellic acid.

Changes in Catch Rate of Monochamus saltuarius (Coleoptera: Cerambycidae) Relation to Sexual Maturation (북방수염하늘소(딱정벌레목: 하늘소과)의 성적 성숙에 따른 포획 효율의 변화)

  • Jung, Jong-Kook;Kwon, Hyeokjun;Kim, Hwang;Kim, Junheon;Nam, Youngwoo;Kim, Dongsoo;Jung, Chansik
    • Korean journal of applied entomology
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    • v.59 no.4
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    • pp.295-301
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    • 2020
  • In this study, we aimed to investigate the changes in the catch rate of Monochamus saltuarius, based on sexual maturation by using aggregation-sex pheromone traps. Ovariole development of caught M. saltuarius females was compared to that of the ones not caught using traps. In a mesh cage set up at the Hongneung experimental forest, we placed a multi-funnel trap with or without an aggregation-sex pheromone lure. M. saltuarius adults, which emerged from pine logs, were grouped in four according to the emergence dates (0, 1, 7, and 10 days after emergence [DAE]). We released beetles into the mesh cage to investigate the catch rate using the traps. In each group, a total of 80 beetles (20 beetles × 4 replications) were tested, making it a total of 320 beetles. Among the four groups, M. saltuarius adults in the 7 DAE group were caught more frequently using the traps, especially with a pheromone lure; the other groups showed a low catch rate. A similar number of female and male beetles were caught using the traps. Regarding ovariole development, all the female adults in the 0 and 1 DAE groups were immature, while those in the other two groups were completely developed. Therefore, aggregation-sex pheromone traps might have a limitation in the prevention of pine wilt disease because of the transmission of pine wood nematode during maturation feeding of newly emerged M. saltuarius adults. However, aggregation-sex pheromone traps can be effective for collecting sexually mature M. saltuarius adults, for the investigation of seasonal occurrence of beetles in forests.

Effect of Different Substances on Composting of Poultry Manure (부재료가 돈분뇨 퇴비화에 미치는 영향)

  • Yoon, Yong-Cheol;Lee, Min-Ho;qasim, Waqas;Lee, Yong-Jin;Kim, Won-Joong;Lee, Jong-Goo;Kim, Hyeon-Tae
    • Journal of agriculture & life science
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    • v.53 no.1
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    • pp.117-126
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    • 2019
  • This paper presents an experiment to examine the possibility of treating carcass while making compost with a horizontal cylinder composting equipment. The findings were as follows: as for heavy metal content, zinc content was above the allowed level in some measuring sections of the general operation and the entire measuring sections of the carcass operation, whose copper content was higher than the general operation. The wood chip operation was lower than the allowed level in all the heavy metals and similar or the same as the carcass operation in copper, cadmium and arsenic. Its nickel content was 29.5~63.8%, which was relatively higher than 9.3~18.0% and 15.8~18.0% of the general and carcass operation, respectively. Its chrome content was 14.2~31.9%, which was relatively higher than that of the general and carcass operation. The integrated operation was lower than the allowed level in all the heavy metals. Its copper and zinc content was 34.9~54.5% and 53.1~75.9%, which was similar to 48.9~52.6% and 64.6~85.9%, respectively, in the wood chip operation. Its chrome and nickel content was stabilized while their content was rather high in the wood chip operation. The average and final moisture content of the carcass operation was 60.7% and 49.6%, respectively. Its average moisture content was a little bit higher than the recommended level of 55.0%, but its final moisture content was lower than the recommended level. The average and final moisture content of the integrated operation was 29.2% and 18.6%, respectively, which was the lowest level among the four operation modes. The overall moisture content of the operation modes was under the recommended level of 55.0% with some variance among the modes. The C/N ratio was the lowest in the carcass operation and the highest in the general operation. The average C/N ratio of the operation modes was in the range of 13.7~20.3 with the total average of 18.3, and the modes kept it under the proper level of 30.0.

Growth of Tomato and Pepper Grafted Plug Seedlings under Different Shading Condition During Acclimatization after Graft-taking (접목활착 후 순화시 차광조건에 따른 토마토와 고추 접목묘의 생육)

  • Jo, Hyeon Gyu;Jeong, Hyeon Woo;Lee, Hye Ri;Kwon, Su Min;Hwang, Hee Sung;Hwang, Seung Jae
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.10-18
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    • 2021
  • Acclimatization after grafting and graft-take that in order to produce plug seedlings of high-quality are important plug seedling stage which not reduce the plug seedlings quality before shipment. Appropriate acclimatization environment can not only increase seedling quality before secondary growth period, but also effective in promoting the growth of plug seedlings. This study was conducted to determine the optimal the shading period and shading level for acclimatization of tomato and pepper grafted plug seedlings after graft-take. The tomato and pepper seedlings were used in this experiment, and after graft-take, a tunnel was installed on a bed in glasshouse of venlo type to started shading treatment. The shading levels were 35, 55, 75 and 95%, and the shading periods were 1 and 2 weeks, and non-treatment was set as the control. In the case of tomato grafted plug seedling, plant height, stem diameter, dry weight of root, leaf area were significantly higher at the shading period of 1 week and the shading level was 55%. In the case of pepper grafted plug seedling, plant height, stem diameter, and leaf area were the highest in the shading period of 2 weeks and the shading level was 35%. However, dry weight of root, compactness, and T/R ratio, which seedling quality indicators, were lower than in the shading period of 1 week and the shading level 55%. Therefore, considering the quality of seedlings, it is suggested that shading period of 1 week with shading level of 55% treatment can be suitable to produce high quality grafted plug seedlings of tomato and pepper.

Study of Minimum Passage Size of Subterranean Termites (Reticulitermes speratus kyushuensis) (국내 흰개미(Reticulitermes speratus kyushuensis)의 최소 통과 직경 연구)

  • Kim, Sihyun;Lee, Sangbin;Lim, Ikgyun
    • Korean Journal of Heritage: History & Science
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    • v.53 no.4
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    • pp.188-197
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    • 2020
  • Termites play an important role as decomposers of the forest ecosystem, while simultaneously causing enormous damage to wooden structures. Currently, two species of subterranean termites have been reported in Korea, and termite damage to historical wooden buildings is occurring nationwide due to climate change, forest fertility, and the locational characteristics of historical wooden buildings. Subterranean termites make their nests underground or inside timber. Termites move underground and access wooden structures through the lower parts of the buildings, adjacent to the ground. Once termites attack the wooden structures, it not only spoils the authenticity of cultural heritage structure, but also hampers structural stability due to the decrease in the strength of the material. Therefore, it is important to prevent termite damage before it occurs. Chemical treatments are mainly used in Korea to control and prevent the damage. In foreign countries, physical barriers are also used to prevent entry to wooden buildings, along with chemical treatments. Physical barriers involve installing nets or particles that termites cannot pass through in the lower part of the building, around the pipes, and between the edges of the building or exterior walls and interior materials. Advantages of a physical barrier are that it is an eco-friendly method, maintains long-term effect after installation, and does not require the use of chemical treatments. Prior to applying physical barriers, studies into the characteristics of termite species must be undertaken. In this study, we evaluated the minimum passage size that each caste of Reticulitermes speratus kyushuensis can move through. We found that workers, soldiers, and secondary reproductive termites were able to pass through diameters of 0.7mm, 0.9mm, and 1.1mm respectively. Head height of termites was an important factor in determining the minimum passing size. Results from the current study will be used as a basis to design the mesh size for physical barriers to prevent damage by termites in historical wooden buildings in Korea.

Effects of Light Intensity and Electrical Conductivity Level on Photosynthesis, Growth and Functional Material Contents of Lactuca indica L. 'Sunhyang' in Hydroponics (수경재배에서 광도와 양액 농도가 베이비 산채 왕고들빼기 '선향' 광합성과 생육 및 기능성 물질 함량에 미치는 영향)

  • Kim, Jae Kyung;Jang, Dong Cheol;Kang, Ho Min;Nam, Ki Jung;Lee, Mun Haeng;Na, Jong Kuk;Choi, Ki Young
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.1-9
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    • 2021
  • This study was conducted to examine the changes of photosynthesis, growth, chlorophyll contents and functional material contents in light intensity and EC concentration of wild baby leaf vegetable, Indian lettuce (Lactuca indica L. cv. 'Sunhyang') in DFT hydroponics. The cultivation environment was 25±1℃ of temperature and 60±5% of relative humidity in growth system. At 14 days after sowing, combination effect of light intensity (Photosynthetic Photon Flux Density (PPFD 100, 250, 500 µmol·m-2·s-1) and EC level (EC 0.8, 1.4, 2.0 dS·m-1) of nutrient solution was determined at the baby leaf stage. The photosynthesis rate, stomatal conductance, transpiration rate and water use efficiency of Indian lettuce increased as the light intensity increased. The photosynthesis rate and water use efficiency were highest in PPFD 500-EC 1.4 and PPFD 500-EC 2.0 treatment. The chlorophyll content decreased as the light intensity increased, but chlorophyll a/b ratio increased. Leaf water content and specific leaf area decreased as light intensity increased and a negative correlation (p < 0.001) was recognized. Plant height was the longest in PPFD 100-EC 0.8 and leaf number, fresh weight and dry weight were the highest in PPFD 500-EC 2.0. Anthocyanin and total phenolic compounds were the highest in PPFD 500-EC 1.4 and 2.0 treatment, and antioxidant scavenging ability (DPPH) was high in PPFD 250 and 500 treatments. Considering the growth and functional material contents, the proper light intensity and EC level for hydroponic cultivation of Indian lettuce is PPFD 500-EC 2.0, and PPFD 100 and 250, which are low light conditions, EC 0.8 is suitable for growth.

Effect of Irrigation Starting Point of Soil on Chlorophyll Fluorescence, Stem Sap Flux Relative Rate and Leaf Temperature of Cucumber in Greenhouse (시설 토양 오이재배에서 관수개시점 처리가 광합성 형광반응, 줄기수액흐름 및 엽온에 미치는 영향)

  • An, Jin Hee;Jeon, Sang Ho;Choi, Eun Yong;Kang, Ho Min;Na, Jong Kuk;Choi, Ki Young
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.46-55
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    • 2021
  • This experiment was conducted to investigate the effect on chlorophyll fluorescence, stem sap flux relative rate (SFRR) and leaf temperature of cucumber when irrigation is controlled using a soil moisture tensiometer. Cucumber (Cucumis sativus L.) 'Chungchun' was irrigated of 10-10-20 kPa and 20-10-10 kPa by soil starting point of irrigation at each growth stage. At the 66 days after treatment (DAT) of 736 to 854 W·m-2 and above 32℃, chlorophyll fluorescence variables (Fo, Fm, Fv/Fm) values showed significantly different between treatments. The Fo and Fv/Fm value in the daytime (10:30 am to 6:00 pm) at 66 DAT was higher in 20-10-10 kPa treatment than in 10-10-20 kPa treatment. The Fv/Fm value decreased when the leaf temperature was increased. There was no difference in leaf growth (length, width and area) at 28 and 66 DAT, but the chlorophyll content (SPAD value) was significantly higher in 20-10-10kPa treatment. SFRR and leaf temperature increased with light intensity and temperature increased. In both treatments, the SFRR started to increase sharply between 8 am and 9 am when the solar radiation is 170 W·m-2 or higher. The soil temperature of the treatments decreased after irrigation, that showed 31.0℃ at 10-10-20kPa and 28.5℃ at 20-10-10kPa on July 5 (820W·m-2 at 1 pm). However, there was no difference in SFRR, leaf temperature, temperature difference (leaf temperature - air temperature) and VPD between treatments. SFRR was significantly positive correlate with the leaf temperature (p < 0.01, r = 0.770). The SFRR and leaf temperature showed positive significant correlation with solar radiation, temperature, soil temperature, soil moisture content and VPD. There was a negative significant correlation with relative humidity and temperature difference.

Are you a Machine or Human?: The Effects of Human-likeness on Consumer Anthropomorphism Depending on Construal Level (Are you a Machine or Human?: 소셜 로봇의 인간 유사성과 소비자 해석수준이 의인화에 미치는 영향)

  • Lee, Junsik;Park, Do-Hyung
    • Journal of Intelligence and Information Systems
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    • v.27 no.1
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    • pp.129-149
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    • 2021
  • Recently, interest in social robots that can socially interact with humans is increasing. Thanks to the development of ICT technology, social robots have become easier to provide personalized services and emotional connection to individuals, and the role of social robots is drawing attention as a means to solve modern social problems and the resulting decline in the quality of individual lives. Along with the interest in social robots, the spread of social robots is also increasing significantly. Many companies are introducing robot products to the market to target various target markets, but so far there is no clear trend leading the market. Accordingly, there are more and more attempts to differentiate robots through the design of social robots. In particular, anthropomorphism has been studied importantly in social robot design, and many approaches have been attempted to anthropomorphize social robots to produce positive effects. However, there is a lack of research that systematically describes the mechanism by which anthropomorphism for social robots is formed. Most of the existing studies have focused on verifying the positive effects of the anthropomorphism of social robots on consumers. In addition, the formation of anthropomorphism of social robots may vary depending on the individual's motivation or temperament, but there are not many studies examining this. A vague understanding of anthropomorphism makes it difficult to derive design optimal points for shaping the anthropomorphism of social robots. The purpose of this study is to verify the mechanism by which the anthropomorphism of social robots is formed. This study confirmed the effect of the human-likeness of social robots(Within-subjects) and the construal level of consumers(Between-subjects) on the formation of anthropomorphism through an experimental study of 3×2 mixed design. Research hypotheses on the mechanism by which anthropomorphism is formed were presented, and the hypotheses were verified by analyzing data from a sample of 206 people. The first hypothesis in this study is that the higher the human-likeness of the robot, the higher the level of anthropomorphism for the robot. Hypothesis 1 was supported by a one-way repeated measures ANOVA and a post hoc test. The second hypothesis in this study is that depending on the construal level of consumers, the effect of human-likeness on the level of anthropomorphism will be different. First, this study predicts that the difference in the level of anthropomorphism as human-likeness increases will be greater under high construal condition than under low construal condition.Second, If the robot has no human-likeness, there will be no difference in the level of anthropomorphism according to the construal level. Thirdly,If the robot has low human-likeness, the low construal level condition will make the robot more anthropomorphic than the high construal level condition. Finally, If the robot has high human-likeness, the high construal levelcondition will make the robot more anthropomorphic than the low construal level condition. We performed two-way repeated measures ANOVA to test these hypotheses, and confirmed that the interaction effect of human-likeness and construal level was significant. Further analysis to specifically confirm interaction effect has also provided results in support of our hypotheses. The analysis shows that the human-likeness of the robot increases the level of anthropomorphism of social robots, and the effect of human-likeness on anthropomorphism varies depending on the construal level of consumers. This study has implications in that it explains the mechanism by which anthropomorphism is formed by considering the human-likeness, which is the design attribute of social robots, and the construal level of consumers, which is the way of thinking of individuals. We expect to use the findings of this study as the basis for design optimization for the formation of anthropomorphism in social robots.

Effects of Stocking Density on the Growth Performance, Immune Status and Breast Meat Quality of Broiler (사육 밀도가 육계 생산성, 면역 수준 및 계육 품질에 미치는 영향)

  • Kim, Hee-Jin;Jeon, Jin-Joo;Kim, Hyun-soo;Son, Jiseon;Kim, Kwang-Yeol;You, Are-Sun;Hong, Eui-Chul;Kang, Bo-seok;Kang, Hwan-Ku
    • Korean Journal of Poultry Science
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    • v.48 no.1
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    • pp.13-22
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    • 2021
  • The present experiment was conducted to evaluate the effect of stocking density on the growth performance, immune status, and meat quality of broilers. In total, 385 one-day-old Ross 308 broilers were randomly assigned to one of four distinct stocking densities: 26 birds/㎡, 22 birds/㎡, 19 birds/㎡, and 16 birds/㎡. They were fed the diet ad libitum for 5 weeks. Immunoglobulin (Ig) and corticosterone levels were evaluated, and growth performance, blood parameters, and breast meat quality were determined. It was observed that the weight gain and feed intake of growers (21~35 d) and broilers (0~35 d) were significantly reduced as the stocking density increased (P<0.05). However, the feed intake of starters (0~21 d) significantly increased as the stocking density increased (P<0.05). There were no significant differences in the biochemical profiles among the four different stock densities. Furthermore, no significant differences were observed in the stress parameters: (heterophils / lymphocytes ratio and corticosterone), IgA, and IgM; however, IgG significantly increased with stocking density (P<0.05). The pH, water holding capacity, and cooking loss of the muscle were all unaffected by the stocking density, but the shear force (tenderness) increased slightly as the density increased. The findings of this study suggest that a lower stocking density (16 birds/㎡) significantly improved the shear force of breast meat and IgG in broilers.