• Journal of Plant Biotechnology
• /
• v.45 no.4
• /
• pp.382-391
• /
• 2018

The objective of this study was to determine the most effective medium condition of shoot proliferation and root formation for the efficient in vitro micropropagation of M.26 (Malus pumila Mill). Simple sequence repeat (SSR) markers were used to analyze the genetic diversity of micro-propagated and greenhouse grown M.26. Shoot proliferation was carried out in MS (Murashige and Skoog) containing benzyladenin (BA, $0.5{\sim}5.0mg{\cdot}L^{-1}$) and thidiazuron (TDZ, $0.01{\sim}0.1mg{\cdot}L^{-1}$). The highest number of shoots (10.67 shoots per explant) was induced by adding BA at a concentration $1.0mg{\cdot}L^{-1}$. TDZ treatments caused higher hyperhydricity rate in cultured explants than in BA treatments. There was no significant effect of both BA and auxin on shoot proliferation, and the optimum proliferation medium for M.26 was MS medium containing $1.0mg{\cdot}L^{-1}$ BA. To find a suitable medium composition for shoot rooting, we tested different concentrations indole-3-butyric acid (IBA) and ${\alpha}$-naphthaleneacetic acid ($0.5{\sim}5.0mg{\cdot}L^{-1}$), MS medium (1/4-1), sucrose ($0{\sim}30g{\cdot}L^{-1}$). The shoots showed good rooting on half-strength MS medium containing $1.0mg{\cdot}L^{-1}$ IBA and $15-20g{\cdot}L^{-1}$ sucrose. The rooting rate (100%), number of roots (10.45 ~ 13.60 roots per explant), root length (7.41 ~ 8.33 cm), and shoot length (4.93 ~ 5.38 cm) were good on this medium. Fifteen SSR primers were detected in a total of 30 alleles in 20 micro-propagated plantlets, all SSR profiles from micro-propagated plantlets were monomorphic and similar to greenhouse grown control plantlet M.26 plant. The results indicated that M.26 micro-propagated plantlets were genetically stable.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.29 no.1
• /
• pp.28-41
• /
• 2024

The carbon balance serves as a valuable indicator of a plant's physiological status under diverse environmental conditions. We investigated the photosynthetic and respiratory responses of the Asian surfgrass Phyllospadix iwatensis along the northeast coast of the Korean peninsula in response to changing water temperature (ranging from 5℃ to 30℃) to estimate the seasonal whole-plant carbon balance through a series of incubation experiments. The maximum gross photosynthetic rate (P_max) showed a significant difference among the temperature treatments, while there was no significant difference in photosynthetic efficiency (α). The maximum gross photosynthetic rate of P. iwatensis reached its peaks at 20℃ treatment (101.65 μmol O₂ g^-1 DW h^-1) but decreased rapidly at 30℃. The saturation irradiance (I_k), compensation irradiance (I_c), and respiration rate (R) of P. iwatensis exhibited significant differences among the temperature treatments. The saturation irradiance increased up to 20-25℃ (121.59-124.50 μmol photons m^-2 s^-1) and sharply decreased at 30℃. The compensation irradiance and respiration rate increased steadily with rising water temperature. The ratio of P_max to R (P_max:R ratio) was the highest at 5℃ but dramatically decreased at 30℃. The whole-plant carbon balance, calculated based on photosynthetic parameters, respiration rates, and biomass, exhibited distinct seasonal variation, increasing during winter and spring and decreasing during summer and fall, which is consistent with the highest in situ growth in spring and severely limited growth at the highest water temperature conditions. Phyllospadix iwatensis displayed a negative carbon balance during late summer, fall, and winter, but demonstrated a positive carbon balance during spring and early summer. Our findings suggest that the rising seawater temperatures associated with climate change may lead to significant alterations in the seagrass ecosystem functioning along the rocky shores of the Korean east coast.

• The Korean Journal of Ecology
• /
• v.23 no.3
• /
• pp.241-253
• /
• 2000

The degree to which microenvironmental factors are linked to spatial patterns of vegetational factors within ecosystems has important consequences for our understanding of how ecosytems are structured and for conservation of rare species in ecosystems. We studied this relationships between the spatial patterns of microenvironmental factors and vegetational factors in temperate hardwood forest in Mt. Jumbong Biological Reserve Area, Korea. To do this, environmental and vegetational factors from 196 micropoints in a 0.49 ha plot were investigated. Most of all environmental factors and vegetational factors showed the variations among micropoints. Microtopographic factors, litter depth, soil moisture content and relative light intensity at this site were spatially dependent at a scale of 14∼62 m. Coverage of tree and shrub layer and species diversity of herb layer in autumn were spatially dependent at a scale of < 15 m. Species richness and species diversity of herb layer in spring and species richness of herb layer in autumn were spatially dependent at a scale of 28∼48 m. Multiple regression analysis showed that spatial patterns of species richness and species diversity of herb layer in spring and autumn were affected by litter depth, slope, subtree layer, shrub, Sasa borealis etc. The best predictor for the spatial patterns of species richness and species diversity of herb layer at this site was the spatial pattern of litter depth. Species richness and species diversity of herb layer showed strongly negative correlation with litter depth. We estimate that the spatial pattern of litter depth at this site were affected by direction of wind, microtopography and spatial pattern of shrub layer.

• Korean Journal of Remote Sensing
• /
• v.33 no.6_1
• /
• pp.947-960
• /
• 2017

Soil moisture plays an important role to affect the Earth's radiative energy balance and water cycle. In general, satellite observations are useful for estimating the soil moisture content. Passive microwave satellites have an advantage of direct sensitivity on surface soil moisture. However, their coarse spatial resolutions (10-36 km) are not suitable for regional-scale hydrological applications. Meanwhile, in-situ ground observations of point-based soil moisture content have the disadvantage of spatially discontinuous information. This paper presents an experimental soil moisture retrieval using Sentinel-1 SAR (Synthetic Aperture Radar) with 10m spatial resolution for cropland in South Korea. We developed a soil moisture retrieval algorithm based on the technique of linear regression and SVR (support vector regression) using the ground observations at five in-situ sites and Sentinel-1 SAR data from April to October in 2015-2017 period. Our results showed the polarization dependency on the different soil sensitivities at backscattered signals, but no polarization dependence on the accuracies. No particular seasonal characteristics of the soil moisture retrieval imply that soil moisture is generally more affected by hydro-meteorology and land surface characteristics than by phenological factors. At the narrower range of incidence angles, the relationship between the backscattered signal and soil moisture content was more distinct because the decreasing surface interference increased the retrieval accuracies under the condition of evenly distributed soil moisture (during the raining period or on the paddy field). We had an overall error estimate of RMSE (root mean square error) of approximately 6.5%. Our soil moisture retrieval algorithm will be improved if the effects of surface roughness, geomorphology, and soil properties would be considered in the future works.

• Korean Journal of Microbiology
• /
• v.41 no.3
• /
• pp.208-215
• /
• 2005

This study was accomplished in order to collect fundamental data on microbial roles in recycling process of reed rhizosphere. Sunchon bay, which is considered as one of the marsh and mud environments severely affected by human activities such agriculture and fisheries, was selected as a model place. In our initial efforts, two bacterial consortia were obtained by enrichment culture using PAH mixtures containing anthracene, naphthalene, phenanthrene and pyrene as the sources of carbon and energy, and four pure bacteria capable of rapid degradation of PAH were isolated from them. Four strains designated as SCB1, SCB2, SCB6, and SCB7 revealed by morphological, physiological and molecular analyses were identified as Burkholderia anthina, Alcaligenes sp., Achromobacter xylosoxidans., and Pseudomonas putida, respectively with over $99{\%}$ confidence. Notably, Burkholderia anthina SCB1 and Alcaligenes sp. SCB2 were found to utilize anthracene and pyrene more quickly than naphthalene and phenanthrene, whereas Achromobacter xylosoxidans SCB6 and Pseudomonas putida SCB7 exhibited similar growth and degradation patterns except for pyrene. These facts suggest that the rhizosphere microorganisms capable of PAH degradation might be used to clean up the contamination sites with polycyclic aromatic hydrocarbons.

• Journal of Plant Biotechnology
• /
• v.31 no.1
• /
• pp.1-6
• /
• 2004

Bang-Poong and related species are an important herbal medicine. However, it is difficult to determine the commercial dry material through anatomical and chemotaxonomical characteristics. Here, we used a PCR-based technique for an accurate discrimination of Bang-Poong and related species. With the RAPD primers, 215 RAPDSs(random amplified polymorphic DNAs) were obtained, and 98% of them showed polymorphic patterns. RAPDs from the four primers were appropriate for the discrimination of S. divaricata $(T_{URCZ{\cdot}})\;S_{CHISKIN}$, those from the six primers for P. japonicum $T_{HUNBERG}$, those from the four primers for P. terebinthaceum $F_{ISHER}$, and those from the six primers for G. littoralis Fr. $S_{CHMIDT}$. The specific bands from the primer 425 were obtained and used to develop SCAR (sequence characterized amplified region) markers, based on the sequence information of the RAPD markers. The SCAR primers generated a 215 bp fragment specific to Peucedanum terebinthaceum $F_{ISHER}$, and a 177 bp and a 300 bp fragment specific to G. littoralis Fr. $S_{CHMIDT}$. As a result, the three SCAR markers were able to discriminate from two Bang-Poong related species.

• Proceedings of the Korean Society of Dyers and Finishers Conference
• /
• 2012.03a
• /
• pp.62-62
• /
• 2012

지구온난화 및 환경오염의 영향으로 선진국을 중심으로한 환경규제가 심해지면서, 홈 텍스타일 분야에서는 세계 패션 트렌드 및 소비자 선호에 부응한 친환경 섬유소재 개발, 웰빙 시대에 적합한 기능성 및 고감성 제품개발을 통한 차별화가 요구되고 있다. 최근의 섬유산업의 동향도 인체에 무해한 천연적인 섬유소재에 많은 관심이 증대됨에 따라 개인의 건강 뿐만 아니라 환경을 생각하는 생활패턴인 친환경섬유의 개발이 새로운 트렌드로 떠오르고 있는 실정이다. 헴프는 일년생 식물로서 학명은 Cannabis sativa L.이다. 헴프섬유의 장점으로 내구성 및 내수성, 항균성 등이 우수한 것으로 보고되고 있으나 양질의 원료 확보, 세섬도 추출 기술 및 combing 기술 등의 부족으로 100% 헴프 세 번수 방적사의 제조가 어려워 주로 면섬유와의 혼합소재로 제조되어 왔다. 최근 들어, 친환경 소재로서 박테리아 성장 억제 기능을 가진 재생섬유인 Tencel 소재를 이용하여 stiff한 Hemp의 성질에 유연성을 추가하여 촉감을 개선함과 동시에, Tencel과 Hemp를 혼용함으로써 soft touch부터 harsh touch까지 혼용율에 의한 다양한 감성을 느끼게 함으로써 용도의 다양화 추구가 시도되어 왔다. Hemp의 거친 느낌을 완화시키고 Tencel의 박테리아 억제 기능과 Hemp의 항균기능, 방충, 탈취기능이 상호 보완되어 친환경적이고 위생적인 다용도 홈 인테리어 및 가구용 직물 등의 제품으로 Hemp/Tencel 복합사가 많이 사용되고 있다. 본 연구는 Hemp와 Tencel의 혼용율의 변화에 따른 복합사의 물리적 특성을 확인하기 위하여 천연복합 태번수 방적사 최적 사설계 이론을 적용하여 Hemp 섬유 혼용율에 따른 사의 물성분석을 함으로써 Hemp/Tencel 방적사 최적 공정 조건을 결정하기 위한 사설계 이론 결과와 실험결과를 비교 분석하고자 한다. 최적 천연 Hemp복합방적사 사설계의 이론화 및 사 물성 DB화 그리고 태번수 Hemp사의 물성분석 및 이들을 DB화 함으로써 가구용 직물로 많이 사용되는 친환경 Hemp 소재사의 방적성 향상을 꾀하고자 한다. 이를 위해서 제조한 방적사의 Dry heat shrinkage와 Wet heat shrinkage를 측정하여 확인하였고 인장시험기를 이용하여 Tenacity, Initial Modulus, breaking strain을 측정 분석하였다. 방적사의 표면 특성은 영상 현미경 시스템을 사용하여 ${\times}40$ 배율로 측정하여 확인하였다.

• Journal of Bio-Environment Control
• /
• v.6 no.4
• /
• pp.299-309
• /
• 1997

This experiment was conducted to determine the mineral absorption character and develop the optimum composition of nutrient solution for dill(Anethum graveolens L.) in hydroponics. Dill(Anethum graveolens L.) plants were grown in nutrient film technique(NFT) supplied with 1/4, 1/2, and 1 strength of the nutrient solution developed by National Research Station in Japan(HRS). Plants grown in 1/2 strength showed the best growth in plant height, fresh weight, and dry weight compared with those grown in 1/4 or 1 strength. In 1/2 strength solution, pH and EC changed little and proper nutrient contents were observed in the leaves as compared to plant nutrient diagnosis standard. Based on these results, optimum macronutrients were composed by nutrient- water absorption rate(n/w) with 1/2 strength: NO$_2$―N 8.85, NH$_4$―N 0.55, P 2.1, K 6.2, Ca 2.8, and Mg 1.7 me L$^{-1}$ To examine the suitability of the nutrient solution developed(SCU) , dill plants were grown in NFT supplied with two different kinds of solution and concentration. 1/2, 1, 3/2 and 25 of SCU and 1/2S of HRS. Changes of pH and EC were not distinct in 1S, but a significant change of pH was shown in low concentrations-HRS 1/2S and SCU 1/2S. Shoot fresh and dry weight were much higher in the plants grown in SCU IS as compared with HRS 1/2S. There were no significant differences in growth of plants grown in SCU IS, 3/2S, and 25. In addition, nutrient contents in the leaves grown by SCU 1S were in proper levels as compared with plant nutrient diagnosis standard. SCU 1S developed in this experiment was found to be optimum for dill in hydroponics.

• Journal of Bio-Environment Control
• /
• v.17 no.4
• /
• pp.247-251
• /
• 2008

During a 35-day growth cycle, N, P, and K uptake was determined by measuring changes in their contents in culture solutions. At harvest, plants were separated into the roots, base organs and shoot, and dried for tissue analysis for N, P, and K. The uptake rates of N, P, and K followed cyclical patterns that was related to shoot development and harvest, but were independent of the transpiration rate. Uptake of N declined from 5.6 mmol $plant^{-1}$ $day^{-1}$ just prior to the cycle initiation to 4.0 mmol $plant^{-1}$ $day^{-1}$ at day 15. Uptake rate steadily increased as flower stems reached maturity up to 10.3 mmol $plant^{-1}$ $day^{-1}$ day 35. Uptakes rates of P and K followed similar patterns of N uptake. Tissue concentrations of N and P steadily decreased since day 15. Content of K was the lowest at day 20 and steadily increased thereafter. In the root tissue, N and K contents were the lowest at day 15, increased to day 30, and then decreased at day 35. Tissue P content was just a reverse of those of N and K.

• Korean Journal of Soil Science and Fertilizer
• /
• v.43 no.4
• /
• pp.471-477
• /
• 2010

Although the roles of calcium in plant are widely known, little is known about on an antagonistic effect of macro elements, oxalate biosynthesis and main shape of crystal in cucumber plant organs. Seeds of cucumber (Cucumis sativus cv. Ijoeunbackdadagi) were germinated in perlite tray supplied with distilled-deionized water. Seedlings were transplanted into aerated containers with a half strength of Ross nutrient solution. Ca levels treated in media were as follows; No-Ca, $Ca(NO_3)_2$ 0.25, 1.25 and 2.5 mmol $L^{-1}$, and $Ca(NO_3)_2$ 2.5 mmol $L^{-1}$ + $CaCl_210$, 25 and 50 mmol $L^{-1}$. Ca-deficient and -excessive conditions severely reduced cucumber growth, as compared to the control, and adversely affected an accumulation of macro elements (N, P, K, and Mg). Calcium favorably induced oxalate (acid-soluble) synthesis in leaves and roots of cucumber plant, but not in stem. Acid-soluble oxalate contents in leaves proportionally increased with Ca supply levels (0.91, P<0.001), however, this pattern was not observed in stem and roots. Ca-oxalate crystal formation and compositional analysis were examined using SEM-EDS technique in cucumber leaves. The main type of crystal revealed a prismatic crystal and main components were Ca, Na and Cl.