Browse > Article

Influence of Calcium Supply on the Growth, Calcium and Oxalate Contents, Mineral Nutrients and Ca-oxalate Crystal Formation of Cucumber  

Sung, Jwa-Kyung (Division of Soil and Fertilizer Management, NAAS, RDA)
Lee, Su-Yeon (Division of Soil and Fertilizer Management, NAAS, RDA)
Lee, Ye-Jin (Division of Soil and Fertilizer Management, NAAS, RDA)
Kim, Rog-Young (Division of Soil and Fertilizer Management, NAAS, RDA)
Lee, Ju-Young (Division of Soil and Fertilizer Management, NAAS, RDA)
Lee, Jong-Sik (Division of Soil and Fertilizer Management, NAAS, RDA)
Jang, Byoung-Choon (Division of Soil and Fertilizer Management, NAAS, RDA)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.43, no.4, 2010 , pp. 471-477 More about this Journal
Abstract
Although the roles of calcium in plant are widely known, little is known about on an antagonistic effect of macro elements, oxalate biosynthesis and main shape of crystal in cucumber plant organs. Seeds of cucumber (Cucumis sativus cv. Ijoeunbackdadagi) were germinated in perlite tray supplied with distilled-deionized water. Seedlings were transplanted into aerated containers with a half strength of Ross nutrient solution. Ca levels treated in media were as follows; No-Ca, $Ca(NO_3)_2$ 0.25, 1.25 and 2.5 mmol $L^{-1}$, and $Ca(NO_3)_2$ 2.5 mmol $L^{-1}$ + $CaCl_210$, 25 and 50 mmol $L^{-1}$. Ca-deficient and -excessive conditions severely reduced cucumber growth, as compared to the control, and adversely affected an accumulation of macro elements (N, P, K, and Mg). Calcium favorably induced oxalate (acid-soluble) synthesis in leaves and roots of cucumber plant, but not in stem. Acid-soluble oxalate contents in leaves proportionally increased with Ca supply levels (0.91, P<0.001), however, this pattern was not observed in stem and roots. Ca-oxalate crystal formation and compositional analysis were examined using SEM-EDS technique in cucumber leaves. The main type of crystal revealed a prismatic crystal and main components were Ca, Na and Cl.
Keywords
Cucumber; Calcium; Oxalate; Ca-oxalate; Mineral nutrient;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kostman, T.A., N.M. Tarlyn. F.A. Loewus. and V.R. Franceschi. 2001. Biosynthesis of $_{L}-ascorbic$ acid and conversion of carbons 1 and 2 of $_{L}-ascorbic$ acid to oxalic acid occurs within individual calcium oxalate crystal idioblasts. Plant Physiol. 125:634-640.   DOI   ScienceOn
2 DeSilva D.L.R., A.M. Hetherington. and T. A. Mansfield. 1996. Where does all the calcium go?. Evidence of an important regulatory role for trichomes in two calcicoles. Plant Cell and Environment 19:880-886.   DOI   ScienceOn
3 Franceschi, V.R. 2001. Function of calcium oxalalccrystals in plants. Trends Plant Sci. 6:331   DOI   ScienceOn
4 Zindler-Frank, E., R. Honow, and A. Hesse. 2001. Calcium and oxalate content of the leaves of Phaseolus vulgaris at different calcium supply in relation to calcium oxalate crystal formation. J. of Physiol. 158:139-144.
5 Zinder-Frank, E., E. Wichmann, and M. Korneli . 1988, Cells with crystals of calcium oxalate in the leaves of Phaseolus ulgaris - a comparison with those in Canavalia ensiformis. Bot. Acta 101:246-253.   DOI
6 Zindler-Frank. E. 1975. On the formation of the pattern of crystal idioblasts in Canavalia ensiformis D.C.: Vii. Calcium and oxalate content of the leaves in dependence of calcium nutrition. Z. Pflanzenphysiol. 77:80-85.   DOI
7 Walinga, I., W. Van Vark, V.J.G. Houba. and J.J. Vander Lee. 1989. Soil and plant analysis : Part 7. Plant analysis procedures. Wageningen Agricultural Univ., ageningen, The Netherlands pp.264
8 Volk . G.M., V.M. Lynch- Holm, T.A. Kostman,and V.R. Franceschi. 2002. The role of druse and raphide calcium oxalate crystals in tissue calcium regulation in Pistia stratiotes Ieaves. Plant Biol. 4:34-45.   DOI   ScienceOn
9 White. P.J. and M.R. Broadley. 2003. Calcium in plants. Annals of Botany 92:487-511.   DOI   ScienceOn
10 Yu L, X.X. Peng, C. Yang. Y.H. Liu, and Y.P. Fan. 2002. Determination of oxalaic acid in plant tissue and root exudates by reversed phase high performance1iquid chromatography. Chinese Journal of Analytical Chemistry 30:1119-1122.
11 Libert, B. 1981. Rapid determination of oxalate acid by reverse-phase high performance liquid chromatography. Journal of Chromatography 210:540-543.   DOI   ScienceOn
12 Libert. B. and V.R. Franceschi. 1987. Oxalate in crop plants. J. Agric. Food Chem. 35:926-928.   DOI
13 Marschner. H. 1995. Mineral nutrition of higher plants. Acad. Press. London
14 Keates. S.A., N. Tarlyn, F.A. Loewus. and V.R. Franceschi. 2000. $_{L}-Ascorbic$ acid and $_{L}- galactose$ arc sources of oxalic acid and calcium oxalate in Pistia stratiotes., Phytochemistry 53:433-440.   DOI   ScienceOn
15 Sander, D., J. Pelloux, C. Brownlee. and J.F. Harper. 2002. Calcium at the crossroads of signaling. Plant Cell 14:401-417.   DOI
16 Shouwu. G., M.D. Ward. and J.A. Wesson. 2002. Direct visualization of calcium oxalate monohydrate crystallization and dissolution with atomic force microscopy and the role of polymeric additives. Langmuir 18:4284-4291.   DOI   ScienceOn
17 Jauregui-Zuniga, D., J.P. Reyes-Grajeda. J.D. Sepulveda-Sanchez, J.R. Whitaker, and A. Moreno. 2003. Crystallochemical characterization of calcium oxalate crystals isolated from seed coats of Phaseolus vulgaris and leaves of Vitis vinifera. J. of Plant Physiol. 160:239-245.   DOI   ScienceOn
18 Kinzel. H. 1989. Calcium in the vacuoles and cell walls of plant tissue. Flora 182:99-125.   DOI
19 Li. X.X. and V.R. Franceschi 1990. Distribution of peroxisomes and glycolate metabolism in relation to calcium oxalate formation in Lemma minorL. Eur. J. Cell Biol. 51:9-16.
20 Kirkby. E.A. and D.J. Pilbeam. 1984. Calcium as a plant nutrient. Plant Cell and Environment 7:397-405.   DOI
21 Gallaher, R.N. 1975. The occurrence of calcium in plant tissue as crystals of calcium oxalate. Commun Soil Sci. Plant Anal. 6:315-330.   DOI
22 Gallaher, R.N. and J.B. Jones. 1976. Total extractable, and oxalate calcium and other elements in normal and mouse ear pecan tree tissues. J. Am. Soc. Hortic. Sci. 101:6922- 6926.
23 Franceschi. V.R. 1989. Calcium Oxalate formation is a rapid and reversible process in Lemna minor L. Protoplasma 148:130-137.   DOI
24 Hodgkinson, A. 1977. Oxalic acid metabolism in higher plants, in: Hodgkinson, A. (Ed.), Oxalic acid biology and medicine. Academic Press. New York. pp.131-158.
25 Homer, H.T. and E. Zinder-Frank, 1982a. Calcium oxalate crystals and crystal cells in the leaves of Rhynchosia caribaes (Leguminosae: Papilionoideae). Protoplasma 111: 10-18.   DOI
26 Horner, H.T. and E. Zindler-Frank. 1982b. Histochemical. Spectroscopic, and x-ray diffraction identifications of the two hydration forms of calcium oxalate crystals in three legumes and Begonia. Can. J. Bot.60:1021-1027.   DOI
27 Foster, A.S. 1956. Plant idioblasts: remarkable examples of cell specialization. Protoplasma 46: 184-193.   DOI
28 Franceschi, V.R. and H.T. Horner. 1980. Calcium oxalate crystals in plants. Botanical Review. 46:361-427.   DOI
29 Borchert, R. 1985.Calcium-induced patterns of calcium-oxalate crystals in isolated leaflets of gleditsia triacanthos L. and Albizia julibrissin Durazz. Planta 165:301-310.   DOI
30 Borchert. R. 1986. Calcium acetate induces calcium uptake and formation of calcium-oxalate crystals in isolated leaflets of Gleditsia tracanthos L. Planta 168:571-578.   DOI