• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.11
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• pp.70-77
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• 2020

Microalgae are independent organisms that perform photosynthesis and can alter the culture environment to increase accumulation of useful substances derived from microalgae. In this study, cell growth was measured by incubation for 39 days using MBBM, Neo medium, and seven light sources, which is the main factor affecting cell growth of microalgae S. obliquus. In the case of S. oliquus, which grew in MBBM and Neo medium, cell growth was highest under fluorescent light sources and Red2 LED (R660) light sources, and cell growth was lowest under Infra Red LED (R741) light sources. The average cell growth rate was 17.7% for MBBM and 15.4% for Neo. Comparing the effects of dry cell weight of Neo medium containing nutrients on the production of aquatic plants, MBBM and dry cell weight of Neo resulted in higher cell growth than Neo medium under all LED light sources except for Blue LED (B450). This proves that MBBM is more suitable for increasing the cell growth of microalgae than Neo medium and confirms that light source selection is important in the production of useful materials through mass cultivation of microalgae in the future.

• Proceedings of the Korean Nutrition Society Conference
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• 2002.05a
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• pp.60-67
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• 2002

락토페린은 주로 유즙에 많이 포함되어 있으며 인간 분비물 등에서도 발견되는 당단백질로써, 미생물 감염에 대한 방어작용이 있는 것으로 알려져 있다. 락토페린의 미생물에 대한 방어작용은 미생물 성장에 필요한 철이온이 락토페린에 결합하여 성장을 저해하기 때문인 것으로 알려져 있다. 락토페린은 이외에도 염증반응의 조절, 임파세포의 성장촉진 등 면역반응에도 관여하는데 이러한 활성은 철에 결합하는 성질과는 무관하게 일어나며 락토페린이 DNA에 결합하는 성질과 관련이 있는 것으로 추측되어진다. 락토페린은 DNA에 결합하여 유전자의 전사에 관여할 것으로 여겨지는데 그 동안 어떤 유전자의 발현에 관여하는지에 대해서 알려진 바가 없었다. 최근 본 연구팀은 락토페린이 포유세포의 세포유전자의 전사에 관여하는지를 분석한 결과 락토페린 결합부위를 가지고 있는 유전자중의 하나인 인간 인터루킨-1$\beta$ 유전자의 전사를 활성화시킨다는 연구 결과를 보여 주었다. 인간 myelogenous leukaemia 세포주인 K562 세포를 락토페린과 phorbolmyristate acetate(PMA)로 함께 처리하면 K562 세포의 인터루킨-1$\beta$ mRNA의 양은 PMA 단독으로 처리하였을 때 보다 상승적으로 더 많이 유도됨을 보여주었다. 또한 IL-1$\beta$/Luciferase 융합 유전자를 K562 배양세포에 넣어 전사 활성을 비교함으로써 락토페린에 의한 인터루킨-l$\beta$의 전사활성을 확인하였다. 락토페린을 전체, N-말단, 혹은 C- 말단 부위를 COS-1 세포에 발현시켜 전사 활성을 측정한 결과 C-말단 쪽은 전사활성이 없었으나 N-말단 90개 아미노산 부위(NIa라 명명)가 전사활성을 가지고 있음을 규명하였다. 본 연구결과는 락토페린이 인터루킨-l$\beta$의 유전자의 전사에 역할을 하고 있음을 보여 주고 있으며 또한 인터루킨-1$\beta$의 유전자 외에도 락토페린 결합 부위를 유전자의 조절부위에 포함하고 있는 세포 유전자의 전사도 관여할 수 있음을 제시하고 있다.

• Proceedings of the Korean Nutrition Society Conference
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• 2002.06a
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• pp.613-616
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• 2002

락토페린은 주로 유즙에 많이 포함되어 있으며 인간분비물 등에서도 발견되는 당단백질로써, 미생물 감염에 대한 방어작용이 있는 것으로 알려져 있다. 락토페린의 미생물에 대한 방어작용은 미생물 성장에 필요한 철이온이 락토페린에 결합하여 성장을 저해하기 때문인 것으로 알려져 있다. 락토페린은 이외에도 염증반응의 조절, 임파세포의 성장촉진 등 면역반응에도 관여하는데 이러한 활성은 철에 결합하는 성질과는 무관하게 일어나며 락토페린이 DNA에 결합하는 성질과 관련이 있는 것으로 추측되어진다. 락토페린은 DNA에 결합하여 유전자의 전사에 관여할 것으로 여겨지는데 그 동안 어떤 유전자의 발현에 관여하는지에 대해서 알려진 바가 없었다. 최근 본 연구팀은 락토페린이 포유세포의 세포유전자의 전사에 관여하는지를 분석한 결과 락토페린 결합부위를 가지고 있는 유전자중의 하나인 인간 인터루킨-1$eta$ 유전자의 전사를 활성화시킨다는 연구 결과를 보여 주었다. 인간 myelogenous leukaemia 세포주인 K562 세포를 락토페린과 phorbol myristate acetate(PMA)로 함께 처리하면 K562 세포의 인터루킨-1$\beta$ mRNA의 양은 PMA 단독으로 처리하였을 때 보다 상승적으로 더 많이 유도됨을 보여주었다. 또한 IL-1$\beta$/Luciferase 융합 유전자를 K562 배양세포에 넣어 전사 활성을 비교함으로써 락토페린에 의한 인터루킨-1$\beta$의 전사활성을 확인하였다. 락토페린을 전체, N-말단, 혹은 C- 말단 부위를 COS-1 세포에 발현시켜 전사 활성을 측정한 결과 C-말단 쪽은 전사활성이 없었으나 N-말단 90개 아미노산 부위(NIa라 명명)가 전사활성을 가지고 있음을 규명하였다. 본 연구결과는 락토페린이 인터루킨-I$\beta$의 유전자의 전사에 역할을 하고 있음을 보여 주고 있으며 또한 인터루킨-1$\beta$의 유전자 외에도 락토페린 결합 부위를 유전자의 조절부위에 포함하고 있는 세포 유전자의 전사도 관여할 수 있음을 제시하고 있다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.10
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• pp.1690-1700
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• 2013

The objective of this study is to develop and validate predictive growth models for Bacillus cereus (diarrhea type) vegetative cells, spores and Staphylococcus aureus in preprocessed Namul (bracken and Chwinamul) and root vegetables (bellflower and burdock). For validation of model performance, growth data for S. aureus in preprocessed vegetables were collected at independent temperatures (18 and $30^{\circ}C$) not used in the model development. In addition, model performance of B. cereus (diarrhea type) in preprocessed vegetables was validated with an emetic type of B. cereus strain. In primary models, the specific growth rate (SGR) of the B. cereus spores was faster than that of the B. cereus vegetative cells, regardless of the kinds of vegetables at 24 and $35^{\circ}C$, while lag time (LT) of the B. cereus spores was longer than that of the B. cereus vegetative cells, except for burdock. The growth of B. cereus and S. aureus was not observed in bracken at temperatures lower than 13 and $8^{\circ}C$, respectively. The LT models for B. cereus (diarrhea type) in this study were suitable in predicting the growth of B. cereus (emetic type) on burdock and Chwinamul. On the other hand, SGR models for B. cereus (diarrhea type) were suitable for predicting the growth of B. cereus (emetic type) on all preprocessed vegetables. The developed models can be used to predict the risk of B. cereus and S. aureus in preprocessed Namul and root vegetables at the retail markets.

• Journal of the korean academy of Pediatric Dentistry
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• v.32 no.3
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• pp.395-402
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• 2005

In considering the healing process of injured periodontal tissue, healing rate would be influenced by the cellular activity of periodontal fibroblasts(PDLFs). In addition, the reattachment among PDLFs should be induced for healing process. The purpose of this study was to evaluate the effects of epidermal growth factor(EGF) on the proliferation and attachment of PDLFs and to verify the efficacy of EGF as a storage media or a pre-replantation conditioner of traumatically avulsed tooth. Human recombinant epidermal growth factor(hrEGF) and human periodontal fibroblasts from first premolar were prepared. At first, MTT assay was done to evaluate the toxic effect on human periodontal fibroblast and the maximum cellular growth of EGF. Cellular proliferation rate was then compared between control group and 10ng/ml EGF added group. Also, western blot was done to evaluate the expression of fibronectin in both groups. The results were as follows: 1. From MTT assay, EGF showed no toxic effect on PDL fibroblasts. The highest proliferation was shown at 10ng/ml EGF. 2. In 10ng/ml EGF added group, the degree of proliferation of PDLFs was significantly higher than that in control group. 3. Fibronectin expression of EGF added group was also significantly higher than that of control group. From this study we could conclude that EGF enhanced the regeneration rate of periodontal fibroblast, which could be used as a pretreatment agent or a storage media for traumatically avulsed teeth.

• Radiation Oncology Journal
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• v.17 no.3
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• pp.223-229
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• 1999

Purpose : The expression pattern of c-jun by ionizing radiation according to cell growth state (exponential growth vs. stationary phase) and its relationship with cell cycle redistribution were investigated. Materials and Methods : The exponential growth phase (day 4) and stationary phase (day 9) cells were determined from cell growth curve according to the elapse of days in CaSki. The cells were irradiated using 6 MV X-ray with a dose of 2 Gy at a fixed dose rate of 3 Gy/min. Northern blot analysis was peformed with total cellular RNA and cell cycle distribution was analyzed using flow cytometry according to time-course after irradiation. Results : The maximum expression of c-jun occurred 1 hour after irradiation in both exponential growth and stationary phase cells. After then c-jun expression was elevated upto 6 hours in exponential growth phase cells, but the level decreased in stationary phase cells. Movements of cells from G0-G1 to S, G2-M phase after irradiation were higher in exponential growth phase than stationary phase. Conclusion : c-jun may be involved in the regulation of cellular proliferation according to the growth states after irradiation.

• Polymer(Korea)
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• v.39 no.3
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• pp.412-417
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• 2015

A critical element in tissue engineering approaches is a control of the mechanical properties of polymer scaffolds to regulate cell phenotype, which may lead to clinically successful tissue regeneration. In this study, we hypothesized that gel stiffness could be a key factor to manipulate adhesion and proliferation of different types of cells. RGD-modified alginate gels with various mechanical properties were prepared and used as a substrate for MC3T3-E1 and H9C2 cells. Adhesion and growth rate of MC3T3-E1 cells in vitro were increased in parallel with an increase of gel stiffness. In contrast, those of H9C2 cells were decreased. This approach to control the mechanical properties of polymer scaffolds depending on the cell types may find useful applications in the tissue engineering.

• KSBB Journal
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• v.10 no.3
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• pp.323-334
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• 1995

The effects of glucose on cell growth kinetics, monoclonal antibody productivity, and cell metabolism or hybridoma cells were investigated. The mouse-mouse hybridoma cell line VIII H-8 producing mouse IgG2a was used as a modal system. Glucose showed substrate inhibition type dependence on specific growth raie. The maximum cell density increased as initial glucose concentration increased up to 4 g/$\ell$. Glucose showed a strong influence on cell death kinetics, and an inverse relationship between specific death rate and glucose concentration was found. Cell viability and monoclonal antibody production increased as initial glucose concentration increased. The specific glucose consumption rate increased with glucose concentration, and cumulative specific lactate production rate increased with increasing initial glucose concentration. The overall kinetics of ammonium ion production was almost invariant with respect to initial glucose concentration, while the cumulative specific ammonium ion production rate was dependent on initial glucose concentration.

• KSBB Journal
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• v.4 no.3
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• pp.259-265
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• 1989

In a batch fermentation process using carrot cell suspension culture, the effect of initial concentration of limiting nutrients(glucose and phosphate) on the specific growth rate and cell yield was investigated. The period of exponential growth is about 2 days and the consumption of glucose and phosphate in culture medium was very small when the initial concentrations of glucose and phosphate are 1.49g/1 ~ 3.01g/l and 0.08 ~ 0.32mM respectively. The specific growth rate of cells ranged from TEX>$0.15\;day^{-1}$ to $0.3\;day^{-1}$ irregularly. And the ratio of the initial concentration of glucose to phosphate did not affect the specific growth rate and the cell yield. The increase on cells had linear relationship with the consumption of limiting nutrients. Therefore, the increase of cells was found to be more influenced by the concentration of glucose than that of phosphate.

• Journal of Periodontal and Implant Science
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• v.25 no.3
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• pp.720-732
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• 1995

The use of transforming growth $factor-{\beta}1$ which functions as a potent biologic mediator regulating numerous activities of wound healing has been suggested for the promotion of periodontal regeneration. The mitogenic effects of transforming growth $factor-{\beta}1$ on human periodontal ligament cells and human gingival fibroblasts were evaluated by determining the incorporation of $[^3H]-thymidine$ into DNA of the cells dose-dependently. Cells were prepared with primary cultured fibroblasts and periodontal ligament cells from humans, and used in experiments were the fourth or sixth subpassage. Cells were seeded with serum free Dulbecco's modified Eagle medium containing 0.1% bovine serum albumine. The added concentrations of transforming growth $factor-{\beta}1$ were 0.25, 0.5, 1, 2.5, 5ng/ml and transforming growth $factor-{\beta}1$ were added to the quiescent cells for 24hours, 48hours, 72hours. They were labeled with lnCi/ml $[^3H]$ thymidine for the last 24hour of the each culture. The results were presented as the mean counts per minute (CPM) per well and S.D. of four determinations. The results were as follows. : The DNA synthetic activity of human gingival fibroblasts was increased dose-dependently by transforming growth $factor-{\beta}1$ at 24 hours, 48 hours and 72 hours. The maximum mitogenic effects were at the 48 hour application of transforming growth $factor-{\beta}1$. The DNA synthetic activity was generally more decreased at the 72 hour application than at the 48 hour the application of transforming growth $factor-{\beta}1$. The DNA synthetic activity of human periodontal ligament cells was increased dose-dependently by transforming growth $factor-{\beta}1$ at 24 hours and 48 hours. But the DNA synthetic activity was decreased at 5ng/ml of the 72 hour application. The maximum mitogenic effects were also at the 48 hour application of transforming growth $factor-{\beta}1$. The DNA synthetic activity of human periodontal ligament cells was generally more decreased at the 72 hour application than at the 48 hour application of transforming growth $factor-{\beta}1$. In the comparision of DNA synthetic activity between the human gingival fibroblasts and human periodontal ligament cells, the human gingival fibroblasts had more activity than the human periodontal ligament cells at all time application with the concentration of transforming growth $factor-{\beta}1$. In conclusion, transforming growth $factor-{\beta}1$ has an important roles in the stimulation of DNA synthesis in human periodontal ligament cells and human gingival fibroblasts, which means an increase in collagen synthesizing cells and thus, may be useful for clinical application in periodontal regenerative procedures.