Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

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Growth Characteristics of Microalgae Scenedesmus obliquus by LED Light Source

LED 광원에 따른 미세조류 Scenedesmus obliquus의 성장 특성

  • Yoo, Yong Jin (Department of Physics and Nano-science, Sun Moon University) ;
  • Kim, Song Yi (Department of Food Science, Sun Moon University) ;
  • Lee, Geon Woo (Department of Physics and Nano-science, Sun Moon University) ;
  • Lee, Young Bok (Department of Physics and Nano-science, Sun Moon University) ;
  • Kim, Jin Woo (Department of Food Science, Sun Moon University) ;
  • Kim, Ho Seob (Department of Physics and Nano-science, Sun Moon University)
  • Received : 2020.07.17
  • Accepted : 2020.11.06
  • Published : 2020.11.30
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Abstract

Microalgae are independent organisms that perform photosynthesis and can alter the culture environment to increase accumulation of useful substances derived from microalgae. In this study, cell growth was measured by incubation for 39 days using MBBM, Neo medium, and seven light sources, which is the main factor affecting cell growth of microalgae S. obliquus. In the case of S. oliquus, which grew in MBBM and Neo medium, cell growth was highest under fluorescent light sources and Red2 LED (R660) light sources, and cell growth was lowest under Infra Red LED (R741) light sources. The average cell growth rate was 17.7% for MBBM and 15.4% for Neo. Comparing the effects of dry cell weight of Neo medium containing nutrients on the production of aquatic plants, MBBM and dry cell weight of Neo resulted in higher cell growth than Neo medium under all LED light sources except for Blue LED (B450). This proves that MBBM is more suitable for increasing the cell growth of microalgae than Neo medium and confirms that light source selection is important in the production of useful materials through mass cultivation of microalgae in the future.

미세조류는 광합성을 수행하는 독립영양생물로 배양 환경을 변화시켜 미세조류 유래 유용물질의 축적을 증대시킬 수 있다. 본 연구에서는 미세조류 S. obliquus의 세포 성장에 주요 인자인 배지와 광원의 영향을 평가하기 위해 MBBM, Neo 배지와 7가지의 광원을 사용하여 39일 동안 배양하여 세포 성장을 측정하였다. 그 결과 MBBM과 Neo 배지에서 성장한 S. obliquus의 경우, Fluorescent light 광원과 Red2 LED (R660) 광원에서 세포 성장이 가장 높게 관찰되었고, Infra Red LED (R741) 광원에서는 세포 성장이 가장 낮게 관찰되었다. 평균 세포 성장률은 MBBM 배지에서 17.7 %, Neo 배지에서 15.4 %의 성장률을 확인하였다. MBBM과 수생식물 생산용 영양소를 포함한 Neo 배지의 건조세포무게(dry cell weight)를 비교하였을 때, Blue LED (B450)를 제외한 LED 광원에서 Neo보다 많은 세포 성장이 높은 것이 확인되었다. 이는, MBBM 배지가 Neo 배지보다 미세조류 세포량 증대에 보다 적합함이 증명되어 향후 미세조류 대량배양을 통한 유용물질 생산에 있어 광원 선정이 중요하다는 것이 확인되었다.

Keywords

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