• Title/Summary/Keyword: 세포 변형

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Assessment of the Bioavailability and Nutritive Function of Genetically Modified β-Carotene-biofortified Rice by Using Wistar Rats (유전자변형 β-carotene 강화 쌀의 생체 이용과 영양기능성 탐색)

  • Lee, Young-Tack;Lee, Kyung-Won;Seong, Ki-Seung;Kim, Sung-Soo;Lee, Jong Seok;Lee, Ok-Hwan;Cho, Hyun-Seok;Suh, Seok-Chul;Han, Chan-Kyu
    • Korean Journal of Food Science and Technology
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    • v.46 no.2
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    • pp.213-218
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    • 2014
  • The purpose of this study was to investigate the bioavailability and nutritive functions of Nak-Dong rice or genetically modified ${\beta}$-carotene-biofortified rice (GM rice) in an experimental animal model. Wistar rats fed either GM rice or Nak-Dong rice did not show differences in bioavailability, growth, organ weights, or visceral fat, suggesting that the nutrient content of GM rice is compositionally equivalent to that of conventional Nak-Dong rice. In addition, GM rice showed improved nutritive function in terms of increased defecation, decreased lipids, and decreased blood glucose.

Isolation of Serratia marcescens CK-3 against phytopathogenic fungi and its enzymatic properties (식물(植物) 병원류(病源惟) 사상균(絲狀菌)에 길항력(拮抗力)을 갖는 Serratia marcescens CK-3의 분리(分離) 및 효소적(酵素的) 성질(性質))

  • Kim, Yeong-Yil;Rhee, Young-Hwan;Kim, Kwang-Sik;Park, Hwa-Sung;Chun, Woo-Bock;Lee, Jae-Wha;Kim, Jong-Hyun
    • Applied Biological Chemistry
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    • v.34 no.1
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    • pp.54-60
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    • 1991
  • Serratia marcescens CK-3, decomposing chitin which is a mar component of cell wall in phyitopathogenic fungi, was isolated from the continuous cropping rhizosphere of pepper and cucumber and its enzymatic property was examined. S. marcescens CK-3 was found tn have an tagonistic effects against, Fusarium axysporum and Rhizoctonia solani and to have complex enzyme system such as chitinase, laminarinase, and proteinase. The preferable composition of the medium for production of chitinase was fond and was as follows : colloidal chitin 1.5%, tryptone 0.5%, glucose 1.0%, peptone 0.2%, $MgSO_4{\cdot}7H_2O\;0.1%,\;K_2HPO_4\;0.1%,\;and\;NaCl\;0.1%$(w/v), pH 6.8. The maximum enzyme production was observed after culture of 72 hours at $30^{\circ}C$ using a medium containing the above chemical composition. The optimal pH and temperature for in vitro activity of chitinase from S. marcescens CK-3 were pH 7.5 and $50^{\circ}C$, respectively. The enzyme activity in-creased by metal ions such as$Ag^+$ and $Mn^{++}$.

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Study on Anti-oxidant and Anti-inflammatory Activity of Eggplant-cheongyeolsodokum (가지-청열소독음(淸熱消毒飮)의 항산화 및 항염 효능에 관한 연구)

  • Yoon, Jong-Moon;Kim, Dong-In;Lee, Ji-Hae;Han, So-Jung;Kim, Ha-Eun;Kim, Hyeon-Jeong;Nam, Kyu-Woo;Park, Ji-Yeon;Chi, Gyeong-Yup;An, Bong-Jeun
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.43 no.2
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    • pp.125-135
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    • 2017
  • The purpose of this study is verification of the anti-oxidant effect and anti-inflammatory effect of Eggplant-cheongyeolsodokum composed of 8 herbs (Solanum melongena L., Lonicera japonica Thunb., Glycyrrhiza uralensis Fisch., Ligusticum chuanxiong Hort., Angelica gigas Nakai., Coptis deltoidea C. Y. Cheng et Hsiao., Gardenia jasminoides J. Ellis., Forsythia suspensa Vahl) to confirm the possibilities as useful cosmetic material. We used the modified prescription of 'cheongyeolsodokum' contained in Korean traditional medical book 'Donguibogam' as composition of Eggplant-cheongyeolsodokum and their proportions. Eggplant-cheongyeolsodokum were extracted with hot water, 70% ethanol and then powdered. To confirm anti-oxidant effect, we investigated radical scavenging ability (DPPH, $ABTS^+$, superoxide), superoxide dismutase (SOD)-like activity, total polyphenolic contents. Also to confirm anti-inflammatory effect, we investigated inhibition effect of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, and Inhibition effect of the expression of inflammatory-related proteins (iNOS, COX-2) by western blot analysis. As a result, Eggplant-cheongyeolsodokum showed good anti-oxidant and anti-inflammation effects, we suggest that it can be used as an active ingredient for cosmetics.

Construction of Pseudoalteromonas - Escherichia coli shuttle vector based on a small plasmid from the marine organism Pseudoalteromonas (극지해양 Pseudoalteromonas 유래의 소형 플라스미드에 기반한 Pseudoalteromonas - Escherichia coli 셔틀벡터 제작)

  • Kim, Dockyu;Park, Ha Ju;Park, Hyun
    • Korean Journal of Microbiology
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    • v.52 no.1
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    • pp.110-115
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    • 2016
  • A small plasmid (pDK4) from the Antarctic marine organism Pseudoalteromonas sp. PAMC 21150, was purified, sequenced and analyzed. pDK4 was determined to be 3,480 bp in length with a G+C content of 41.64% and contains three open reading frames encoding a replication initiation protein (RepA), a conjugative mobilization protein (Mob) and a hypothetical protein. PCR-amplified pDK4 was cloned in high-copy pUC19 to yield the fusion vector pDOC153. The chloramphenicol resistance gene was inserted into pDOC153 to give an ampicillin and chloramphenicol-resistant, Pseudoalteromonas - Escherichia coli shuttle vector (7,216 bp; pDOC155). The TonB-dependent receptor (chi22718_IV ) and exochitinase (chi22718_III ) genes from Arctic marine P. issachenkonii PAMC 22718 were cloned into pDOC155 to produce pDOC158 and pDOC165, respectively. Both vector derivatives were transferred into plasmid-free Pseudoalteromonas sp. PAMC 22137 by the triparental mating method. PCR experiments showed that the genes were stably maintained both in Pseudoalteromonas sp. PAMC 22137 and E. coli $DH5{\alpha}$ cells, indicating the potential use of pDOC155 as a new gene transfer system into marine Pseudoalteromonas spp.

Standardization of Preparation of Chinese Cabbage Kimchi I. Effects of Dry Salting of Method Salt Content on the Texture of Kimchi (통배추김치 제조의 표준화 연구 I. 농도별 건염법이 배추 질감에 미치는 영향)

  • 정순애;오현희;전소현;최은정;김병미;조신호
    • The Korean Journal of Food And Nutrition
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    • v.16 no.4
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    • pp.423-429
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    • 2003
  • Kimchi has been worldwide recognized as a traditional Korean fermented food, In the Chinese cabbage's pickling, salt content of evenly penetrate into the tissue affects on the texture of matured kimchi, But the previous method which usually using brine salting has a disadvantage of uneven penetration of salt into the leaves and stems. The purpose of the study is to develope the method which produce superior kimchi by evenly penetrate the salt into the tissue rapidly, The dry salting method is directly spread the salt on the surface of chinese cabbage as percentage in weight with 10, 12, 14, 16%(s) and pickled in this state for 5 hours. Brine salting method is soaking chinese cabbage in the 16% salt solution(16%B) for 20 hours. Sensory evaluation, contents of salt, properties of mechanical hardness and micro structure were studied among fresh cabbage, 16%B and 10, 12, 14, 16%(s) cabbage, The result of salt contents in all groups of pickled chinese cabbage were significantly increased compared with fresh cabbage. The compressive force of cabbage's steams by mechanical measurement was significantly decreased but cutting force was increased. In pickled cabbage, the cutting force was the highest correlated with salt content, In the sensory evaluation, the salty taste of leaves and chewy degree were significantly increased, but the hardness and crips of the stems were significantly decreased by increasing salt contents. Therefore the dry salting is the most excellent pickling method in small amount of kimchi production by saving salt amount and time, furthermore due to even penetration of salt into leaves and stems.

Evaluation of Six Species Ciliates as a Live Food and Culture Environment for Euplotes sp. (먹이생물로써의 섬모충 6종의 평가와 Euplotes sp.의 배양 환경)

  • Yoo Jin Hyung;Hur Sung Bum
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.4
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    • pp.342-347
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    • 2002
  • Ciliates have the possibility of a new live food in marine finfish culture because of their wide range of body size, thin tell wall, show motility, and fast reproduction rate. In this research, six species of ciliates were isolated from south coast and salt pond in Korea. The fitness of these species as a live food was evaluated in terms of size, motility, suspensibility and cell density. As the result, Euplotes sp. (K-1) was found suitable to be a new live food which might substitute rotifers, Brachionus plintilis and B. rotundiformis in fish larvae culture. The modified $F{\emptyset}yn's$ Erdschreiberd media, MErds-2 with the addition of glycine, glucose and yeast extract increased six times higher growth rate of Euplotes sp. (K-1) than the basic F$\emptyset$yn's Erdschreiberd media. The optimum water temperature, pH and light intensity for this ciliates were $22.5^{\circ}C$, 8 and 2,000 lux, respectively, and its culture environmental range was relatively wide, On the other hand, this ciliate fed baker's yeast, Saccharomyces cererisiae grew up to 1,240 inds./mL with the inocula of 100 inds./mL within 7 days. The results of the study showed that Euplotes sp. (K-1) has a potential to be utilized as a new live food in fish larvae culture.

Quality Comparison between Gamma Irradiation and Freeze Drying Methods in Preparing Kimchi for the Long-term Storage (장기저장이 가능한 김치제조를 위한 감마선 조사와 동결건조법의 품질비교)

  • Park, Jin-Gyu;Park, Jae-Nam;Han, In-Jun;Song, Beom-Seok;Kim, Jae-Hun;Lee, Ju-Woon;Hwang, Han-Joon;Kim, Young-Duk;Byun, Myung-Woo
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.9-14
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    • 2008
  • This study compared microbial load, texturre, histological properties, and sensory characteristics of gamma-irradiated (20 kGy) Kimchi and freeze-dried Kimchi under condition; of long-tenn storage. immediately after irradiation, no aerobic bacteria were detected, but microbial load of freeze-dried Kimchi decreased by only 1 log cycle in comparision with the nonirradiated control sample. The firmness of irradiated Kimchi was significantly higher than that of rehydrated freeze-dried Kimchi (p<0.05). Scanning electron microscopy showed collapse of the microstructure in epidermis tissues of rehydrated Kimchi. 'The sensory scores for color, texture, and taste of irradiated Kimchi were higher than those for rehydrated freeze-dried Kimchi.

Anatomical Comparison of Compression, Opposite, and Lateral Woods in a Branch of Pinus parviflora S. et Z. (섬잣나무(Pinus parviflora S. et Z.) 지재의 압축이상재, 측면재, 대응재에 관한 해부학적 특성 비교)

  • Xu, Guang Zhu;Eom, Young Geun
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.1 s.129
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    • pp.38-47
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    • 2005
  • Compression, lateral, and opposite woods in a branch of Pinus parviflora S. et Z. were described and compared in the qualitative anatomical aspects through light and scanning electron microscopy. Tracheid transition from earlywood to latewood in the compression wood appeared to be relatively more gradual than in the opposite or lateral wood. Growth ring width and proportion of latewood were thought to be greater in the compression wood than in the lateral or opposite wood. The latewood tracheids of compression wood in transverse surface were mostly round, differently from those of lateral and opposite woods with square to angular shapes. Also, intercellular spaces, helical cavities and checks, and slit-like pit apertures were observed only in the compression wood tracheids. Cross-field pitting in the compression wood appeared not to be used as diagnostic guide because of their severe alteration from normal fenestriform or window-like type to cupressoid to taxodioid types. In tangential surface, fusiform rays in the compression wood were wider but lower than those in the lateral wood or opposite wood. In conclusion, compression wood was different from lateral and opposite woods but lateral and opposite woods were almost identical in qualitative anatomical features.

Effect of Turbidity Changes on Tissues of Zacco koreanus (탁도 변화가 참갈겨니 (Zacco koreanus) 조직에 미치는 영향)

  • Shin, Myung-Ja;Kim, Jeong-Sook;Hwang, Yun-Hee;Lee, Jong-Eun;Seo, Eul-Won
    • Korean Journal of Ecology and Environment
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    • v.41 no.1
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    • pp.73-80
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    • 2008
  • Present study aims to investigate the effect of muddy water on the gill and the kidney tissues of Zacco koreanus under high turbidity rearing condition. The gill of Z. koreanus showed abnormal shapes in its secondary lamellae and a rough surface with impure debris in the high level of turbidity and the longer raising period condition. In addition, the gills showed the edema, the exfoliation of epithelial cell, and the fusion of the secondary lamellae. In case of kidney tissue, the atrophied glomerulus was observed, and the empty space in Bowman's capsule was wider. The SOD activities in both gill and kidney tissues were increased in proportion to the high level of turbidity. On the while, CAT and GPX activities were shown constant level in the gill, but were increased in the kidney in the high turbid muddy water. These results indicate that the harmful radicals which generate by high level of turbidity could be removed partly by antioxidant enzymes in the kidney. The concentrations of micro heavy metal ions accumulated in the gill increased drastically at the 1,000 NTU. Based on the above results, it is considered that the exposure to the high level of turbidity for long period may affect on the structures of tissues, and change the enzymatic balance in Z. koreanus, causing the fatal disease.

Effects of Ethyl Acetate Fraction from Melothria Heterophylla on Antioxidant Activity and Matrix Metalloproteinase-1 Expression in Ultraviolet A-irradiated Human Dermal Fibroblasts (백렴 에틸 아세테이트 층의 항산화 활성과 Matrix Metalloproteinase-1 발현 저해효과)

  • Cho, Young-Ho;;Sim, Gwan-Sub;Lee, Dong-Hwan;Lee, Bum-Chun;Pyo, Heong-Bae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.31 no.1 s.49
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    • pp.103-109
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    • 2005
  • UV irradiation leads to distinct changes in skin connective tissue, which is degradation of collagen. Many of these alterations in the extracellular matrix are mediated by matrix metalloproteinases. In this study, to develop a new anti-aging agent, we screened the antioxidant activity of solvent fractions from ethanolic extract of Melothria Heterophylla. Among the four solvent fractions tested, the EtOAc fraction exhibited the highest antioxidant activity. It was investigated the inhibitory effect of the EtOAc fraction on the expression and activity of MMP-1 in UVA-irradiated human dermal fibroblasts. The EtOAc fraction inhibited the activity of MMP-1 in a dose dependent manner with the $IC_{50}$ values of $9{\mu}g/mL$. Also, UVA-induced MMP-1 expression was reduced about $90\%$ by $100{\mu}g/mL$ of the EtOAc fraction but MMP-1 mRNA expression was not inhibited. Therefore, we conclude that the EtOAc fraction significantly inhibits MMP-1 expression at the protein level. From these results, we suggest that the EtOAc fraction from M. heterophylla could be used as a new anti-aging agent for the photo-damaged skin.