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http://dx.doi.org/10.5657/kfas.2002.35.4.342

Evaluation of Six Species Ciliates as a Live Food and Culture Environment for Euplotes sp.  

Yoo Jin Hyung (Department of Aquaculture, Pukyong University)
Hur Sung Bum (Department of Aquaculture, Pukyong University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.35, no.4, 2002 , pp. 342-347 More about this Journal
Abstract
Ciliates have the possibility of a new live food in marine finfish culture because of their wide range of body size, thin tell wall, show motility, and fast reproduction rate. In this research, six species of ciliates were isolated from south coast and salt pond in Korea. The fitness of these species as a live food was evaluated in terms of size, motility, suspensibility and cell density. As the result, Euplotes sp. (K-1) was found suitable to be a new live food which might substitute rotifers, Brachionus plintilis and B. rotundiformis in fish larvae culture. The modified $F{\emptyset}yn Erdschreiberd media, MErds-2 with the addition of glycine, glucose and yeast extract increased six times higher growth rate of Euplotes sp. (K-1) than the basic F$\emptyset$yn's Erdschreiberd media. The optimum water temperature, pH and light intensity for this ciliates were $22.5^{\circ}C$, 8 and 2,000 lux, respectively, and its culture environmental range was relatively wide, On the other hand, this ciliate fed baker's yeast, Saccharomyces cererisiae grew up to 1,240 inds./mL with the inocula of 100 inds./mL within 7 days. The results of the study showed that Euplotes sp. (K-1) has a potential to be utilized as a new live food in fish larvae culture.
Keywords
Fish larvae culture; Live food; Ciliates; Euplotes;
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1 笠原正五郎, 平野札次郎, 大島泰雄. 1960. クロダイ人工孵化仔稚の飼育とその成長について. 日本水産學會誌, 26, 184-l93
2 代田昭彦. 1970. 魚類椎存魚期の口徑に關する硏究. 日本水産學會誌, 36, 353-368
3 杉田治男, 岡本尙人, 奧島章兒, 出口吉昭. 1987. 沿岸水域から分離した原生動物の培養條件. 水産增殖, 35, 125-131
4 Poroter, K.G., M.C. Pace and J.F. Battey. 1979. Ciliate protozoans as lines in freshwater planktonic food chains. J. Protozool., 27, l14-125
5 Lee, J.J., A.C. Douglas, M.E. Mcenery and D. Kahan. 1987. A baited-bloom approach to the isolation of protozoa and meiofauna for potential use in mariculture. 40th annual meeting of the society of protozoologists. 22pp
6 Maeda, M. and P.G. Garey. 1985. An illlustrated guide to the species of the Families Halteriidae and Strobilidiidae (Oligotrichida, ciliophora), free swimming protozoa common in the aquatic environment. Bull. Ocean Res. Inst., Univ. Tokyo, 19, 68pp
7 Park, C.H. and S.B. Hur. 2001. Mass culture and dietary value of Fabrea salina. J. Korean Fish. Soc., 34, 32-37 (in Korean)
8 Porter, K.G., M.B. Sherr, B.F. Sheer, M. Pace and R.W. Samolus. 1985. Protozoa in planktonic food webs. J. Protozool., 32, 409-415   DOI
9 Seki, H. 1966. Studies on microbial participation to food cycle in the sea. III. Trial cultivation of brine shrimp to adult in a chemostat. J. Oceanogr. Soc. Japan, 22, 27-32
10 Sheldon, R.W., P. Nival and F. Rassouleadegan. 1986. An experi-mental investigation of a flagellate-ciliate-copepod food chain with some observation relevant to the linear biomass hypothesis. Limol. Oceacogr., 31, 184-188   DOI   ScienceOn
11 Thompson, A.S., J.C. Phosdes and I. Pettman. 1988. Culture collection of algae and protozoa. Natural Environment Research Council. Cumbria. U.K., 164pp
12 Winter, F., G. Persoone and C. Benijts-Claus. 1975. Fabrea salina, A promising live food for mariculture purpose. In Progressing 6th annual workshop of the world mariculture, pp. 429-438
13 萱野泰久. 1988. キジハタ仔稚漁の口器の發達と攝餌. 岡山縣水試報, 3, 55-60
14 Fenchel, T. and P. Harrison. 1976. The significance of bacterial grazing and mineral cycling for the decomposition of particulate detritus, In The role of terrestrial and aquatic organisms in decomposition processes, J.M. Anderson, ed. Blackwell Scientific, Oxford, pp. 295-299
15 木谷甲子男. 1989. 纖毛原生動物の飼料生物化とその培養法. 水産增殖, 89, 314-315
16 Bamdad, M., C.A. Groliere, J. Dupy-Blanc and L. David. 1993. Change in intracellular pH in Tetrahymena pyriformis GL in relation to the toxicity level of the ionophorous antibiotic nigericin. European journal of protistology, 29, 230-234   DOI   ScienceOn
17 Berk, S.G., D.C. Brownlee, D.R. Heinle, H.J. Kling and R.R. Colwell. 1977. Ciliates as a food source for marine planktonic copepods. Microb. Ecol., 4, 27-40   DOI   ScienceOn
18 Guillard, R.R.L. 1973. Division rate. In Handbook of phycological methods-culture method and growth measurements, J.R. Stein, ed. Cambrige Univ. Press, London, pp. 289-311
19 Hoshaw, R.J. and J.R. Rosowski. 1973. Methods for microscopic algae. In Handbook of phycological methods-culture method and growth measurements, J.R. Stein, ed. Cambnge Univ. Press, London, pp. 53-68
20 Jahn, T.L., E.C. Bovee and F.F. Jahn. 1979. How to know the protozoa. Wm. C. Brown Company Publishers. Dubuque, Iowa, 279pp
21 Jost, J.L., J.F. Prake, A.G. Fredrickson and H.M. Tsuchiya. 1973. Interactions of Tetrahymena pyriformis, Escherichia coli, Azotobacter vinelandii and glucose in a minimal medium. J. Protozool., 20, 834-840
22 Kanazawa, A., J. Teshima, S. Inamori, S. Sumida and T. Iwashita. 1982. Rearing of larval red sea bream and ayu with artifical diets. Mem. Fac. Fish., Kagoshima Univ., 31, 185-192
23 Kim, H.S., C.H. Park and S.B. Hur. 2000. Distribution of Fabrea salina at salt pond. J. Fish. Sci. Tech., 3, 222-227