• Journal of fish pathology
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• v.9 no.2
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• pp.147-155
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• 1996

This study was carried out to examine the acute toxic effects of ammonia to the eel, Anguilla japonica in high temperature and pH levels by histopathological observations. The eels of 40 g average body weight were exposed to 4 different concentrations of total ammonia (0, 10, 20, 30 mg/$\ell$) for 24~120 hours. Each concentration was treated under 4 different levels of pH (7.5, 8.0, 8.5, 9.0) and each of these treatments was tested at 2 different temperatures ($27^{\circ}C$, $32^{\circ}C$). Histopathological changes in gill tissues were observed by hematoxylin-eosin stain. As increasing of pH (from 7.5 to 9.0), water temperature (from $27^{\circ}C$ to $32^{\circ}C$), total ammonia concentration (from 0 mg/$\ell$ to 30 mg/$\ell$) and exposure time (from 24 hours to 120 hours), gill discolorated to dark brown with the naked eye and gill tissues showed hypertrophy of gill lamellae, winding of the secondary gill lamellae, epitherial separation and necrosis histopathologically. When gill lamellae epithelium was separated from the blood vessels, gill discolorated to dark brown with the naked eye.

• Journal of Life Science
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• v.17 no.1 s.81
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• pp.97-104
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• 2007

Present study aims to investigate the effect of muddy water on gill and kidney tissues and blood components of Opsariichthys uncirostris amurensis in Imha reservoir. As a result of observing the gill tissues in muddy water with light and scanning electron microscopes, the edema, the exfoliation of epithelial cells and fusion of the secondary lamellae were observed. The space between gill lamellae was irregular, and a lots of muddy debris around the secondary lamellae were observed. In case of kidney tissues, the size of glomerulus was smaller and thickness of basal membrane was broader and the empty space in Bowman's capsule was wider than those of the control. The blood cells of O. uncirostris amurensis in muddy water were increased in number. Of the white blood cells, acidophilic granulocytes were highly distributed, and the shapes were irregular. Among the plasma components, the concentration of TP, ALB, A/G ratio, TG, CHOL, and AST were low, whereas that of the inorganic materials was high. Based on the above results, it is considered that muddy water possibly involves in the decrease of respiratory and excretory rates, giving rise to secondary lesion of tissues.

• The Korean Journal of Malacology
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• v.27 no.3
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• pp.181-190
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• 2011

This study was investigated to find out the change of reproductive and histological biomarkers of Ruditapes philippinarum exposed to nonylphenol (NP). Experimental period was 16 weeks. Experimental groups were composed of one control condition and three NP exposure conditions (0.05, 0.1, $0.2 mg\;NP\;L^{-1}$). Gonad index was higher in exposure group than control in female and higher in control than exposure group in male. Intersexuality in the NP exposure group was 14.56% with male exhibiting higher ratio than female. Histological analysis of organ system illustrated expansion of hemolymph sinus, loss of striated border of inner epidermis and degeneration of mucous cell in the mantle. In the gill partially disappearance of frontal and lateral cilia, degeneration of mucous cell were observed. Also, hyperplasia of epithelium and disappearance of striated border were observed in the foot. As the concentration of NP increased, the accumulation of lipofuscin increased in the digestive gland. The results of this study suggest that NP induces reduction of survival rate, histopathology and intersex of R. philippinarum. Furthermore, it is indentified that NP functions as an estrogenic effector on the reproduction of R. philippinarum.

• Korean Journal of Environmental Biology
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• v.33 no.1
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• pp.7-18
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• 2015

In this study, we studied the effect of dissolved cement powder on Carassius auratus by analysis of the morphophysiological changes. The gill exposed to dissolved cement powder showed the thickened primary lamellae and the activity of chloride cells and mucous cells was also significantly increased and the proliferation, separation and clubbing of gill filament was observed in the secondary lamellae. In the kidney tissue, the space in Bowman's capsule was widen and the arrangement of dermis was irregular due to the thinned epidermis in the integument tissue. The activities of antioxidant enzymes and LDH tended to increase with the duration of cement exposure. It was confirmed that the up-regulated proteins were identified as involved in glycolysis and energy metabolism and down-regulated proteins were myofibrillar proteins which were involved in muscle contraction by the cement exposure to the integument. With these results, dissolved cement powder was thought to be a big threat to the survival of the fish because it causes the morphological changes and weakens the physiological activity in C. auratus tissues.

• Clinical and Experimental Pediatrics
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• v.49 no.3
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• pp.332-336
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• 2006

Toxic epidermal necrolysis (TEN) is a rare, acute and life-threatening cutaneous drug reaction. TEN is characterized by the sudden onset of extensive necrosis in the epidermis and frequent mucous membrane involvement. The pathogenesis has not yet been elucidated. In addition, no particular treatment for TEN has been established. We report a case of TEN in a 14-year-old-boy, which might have been caused by steroids with enalapril treatment for membranous nephropathy. He recovered after intravenous immunoglobulin therapy.

• Korean Journal of Ecology and Environment
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• v.44 no.1
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• pp.95-101
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• 2011

Present study aimed to investigate morphological change and gene expression in the gill of Cyprinus carpio after exposure against muddy water caused by riverbed disturbance with various rearing condition. The gill of C. carpio showed abnormal shapes in its secondary lamellae and a rough surface with impure debris after exposed to muddy water for 80 days. In addition, the gills showed the edema, the exfoliation of epithelial cell, and the fusion of the secondary lamellae. Using 20 ACPs, 24 differentially expressed genes (DEGs) exposed to muddy water for 80 days were identified. 17 genes among them were up-regulated, while 7 genes were down-regulated in preterm deliveries. A BLAST searches revealed that 3 genes were revealed known genes as calcium transporter 1 (TRPV6) mRNA, macha mRNA for putative puroindoline b protein, and Efnb3 protein-like. Therefore, it is considered that Efnb3 gene from gill would be a useful indicator for neurobehavioral changes in fish influenced by muddy water.

• Journal of Life Science
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• v.25 no.6
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• pp.615-623
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• 2015

This study investigated the effects of dissolved cement powder on Zacco koreanus by analyzing morphophysiological changes. The gills exposed to dissolved cement powder developed abnormal shapes in their secondary lamellae and increased numbers of mucous cells after long-term exposure. Additionally, clubbing, edema, and exfoliation of the epithelial cells were observed in the secondary lamellae. In the kidney tissue, the space in Bowman’s capsule was widened, and in the integument tissue the arrangement of the dermis was irregular due to the thinned epidermis. These results suggest that long-term exposure to cement powder can significantly affect morphological change, resulting death. The activities of antioxidant enzymes and LDH tended to increase commensurately with the duration of cement exposure. It was concluded that up-regulated proteins were the stress proteins involved in myofibrillar-protein production and down-regulated proteins were involved in glycolysis and energy metabolism after the integument’s exposure to cement. According to these results dissolved cement powder is a serious threat to the survival of fish because it causes morphological changes and weakens physiological activity in Z. koreanus tissues.

• Journal of Chest Surgery
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• v.30 no.8
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• pp.838-842
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• 1997

Atypical bronchopulmonary carcinoid is an uncommon pulmonary tumor, an intermediate form between low grade malignant typical carcinoid and high grade malignant small cell carcinoma which is considered neuroendocrine in origin and may produce various hormones. We describe an asymptomatic middle aged women with no elevated hormone level. Based on bronchoscopic biopsy initial diagnosis of atypical carcinoid with oncocytic change was established. The patient underwent right lower and middle lobe bilobectomy and medi stinal Iymph node dissection. Postoperative adjuvant radiation therapy was performed in 4 weeks.

• Journal of Life Science
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• v.21 no.8
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• pp.1156-1162
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• 2011

Equine coital exanthema caused by equine herpesvirus type 3 (EHV-3) is a venereal disease which seriously drops horse reproduction rates. Here, we isolated EHV-3 from infected horses and investigated their biological characteristics. Initial cytopathic effects such as rounding of cells were detected 48 hours post infection of the virus into RK-13 cells. The infected cells were going to detach from the surface of culture flasks 72 hours post infection. The type of isolated viruses from swabbed samples was EHV-3 by PCR analysis. Glycoprotein G (gG) of isolated EHV-3 has a 99.25 percent similarity rate to that of EHV-3 334/74 control strain. The isolated EHV-3 was named Georo strain. Georo strain consisted of four major proteins including 145 kD, 60 kD, 45 kD and 40 kD, as shown by SDS-PAGE analysis. We hope the newly isolated Georo strain of EHV-3 can be used for studying various aspects of Korean equine coital exanthema.

• Polymer(Korea)
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• v.30 no.3
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• pp.259-265
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• 2006

Small intestinal submucosa (SIS) consists of some growth factors which can stimulate cell activity, and PVA has been widely utilized in the area of wound dressing as hydrogel which is easy to be removed from wounds. In this study, native SIS sheets were coated with PVA by immersing them into 2, 4, and 10 wt% of PVA solution and then lyophilized on two type of molds to endow the prepared wound dressing with easy removal property from wounds. The mechanical properties were examined through tensile test. Moreover, enzymatic degradation, water uptake, and in vitro test were carried out to characterize the prepared SIS sheets. The tensile strength of the SIS sheets coated with PVA (PVA-SIS) were decreased, whereas the elongation were increased. Degradation ratio of the PVA-SIS sheets was decreased compare to native SIS. Water uptake ability was improved at 2 and 4 wt% of PVA. The degree of fibroblast attachment was lower than the native SIS sheets. In conclusion, this study suggests that the PVA coated SIS sheets have a potential for the applications of wound dressing and biodegradable injectable materials.