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Changes of Gill Structure and Identification of Genes by Muddy Water Exposure in Cyprinus carpio  

Shin, Myung-Ja (Department of Biological Science, Andong National University)
Lee, Jong-Eun (Department of Biological Science, Andong National University)
Seo, Eul-Won (Department of Biological Science, Andong National University)
Publication Information
Korean Journal of Ecology and Environment / v.44, no.1, 2011 , pp. 95-101 More about this Journal
Abstract
Present study aimed to investigate morphological change and gene expression in the gill of Cyprinus carpio after exposure against muddy water caused by riverbed disturbance with various rearing condition. The gill of C. carpio showed abnormal shapes in its secondary lamellae and a rough surface with impure debris after exposed to muddy water for 80 days. In addition, the gills showed the edema, the exfoliation of epithelial cell, and the fusion of the secondary lamellae. Using 20 ACPs, 24 differentially expressed genes (DEGs) exposed to muddy water for 80 days were identified. 17 genes among them were up-regulated, while 7 genes were down-regulated in preterm deliveries. A BLAST searches revealed that 3 genes were revealed known genes as calcium transporter 1 (TRPV6) mRNA, macha mRNA for putative puroindoline b protein, and Efnb3 protein-like. Therefore, it is considered that Efnb3 gene from gill would be a useful indicator for neurobehavioral changes in fish influenced by muddy water.
Keywords
Cyprinus carpio; muddy water; gene expression; gill; DEGs;
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Times Cited By KSCI : 8  (Citation Analysis)
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