Browse > Article
http://dx.doi.org/10.5352/JLS.2011.21.8.1156

Characteristics on Equine Herpesvirus Type 3 from Korea  

Yang, Jae-Hyuk (Jeju Race Park, Korea Racing Authority)
Lim, Yoon-Kyu (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
Publication Information
Journal of Life Science / v.21, no.8, 2011 , pp. 1156-1162 More about this Journal
Abstract
Equine coital exanthema caused by equine herpesvirus type 3 (EHV-3) is a venereal disease which seriously drops horse reproduction rates. Here, we isolated EHV-3 from infected horses and investigated their biological characteristics. Initial cytopathic effects such as rounding of cells were detected 48 hours post infection of the virus into RK-13 cells. The infected cells were going to detach from the surface of culture flasks 72 hours post infection. The type of isolated viruses from swabbed samples was EHV-3 by PCR analysis. Glycoprotein G (gG) of isolated EHV-3 has a 99.25 percent similarity rate to that of EHV-3 334/74 control strain. The isolated EHV-3 was named Georo strain. Georo strain consisted of four major proteins including 145 kD, 60 kD, 45 kD and 40 kD, as shown by SDS-PAGE analysis. We hope the newly isolated Georo strain of EHV-3 can be used for studying various aspects of Korean equine coital exanthema.
Keywords
Characteristics; equine coital exanthema; equine herpesvirus type 3; Korea;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Roizman, B., R. C. Desrosiers, B. Fleckenstein, C. Lopez, A. C. Minson, and M. J. Studdert. 1992. The family herpesviridae: an update. Arch. Virol. 123, 425-449.   DOI
2 Samper, J. C. 2009. Equine breeding management and artificial insemination. pp. 103-161, 2nd eds., Saunders, Missouri.
3 Seki, Y., T. M. Seimiya, G. Yaegashi, S. Kumagai, H. Sentsui, T. Nishimori, and R. Ishihara. 2004. Occurrence of equine coital exanthema in pastured Draft horses and isolation of equine herpesvirus 3 from progenital lesions. J. Vet. Med. Sci. 66, 1503-1508.   DOI
4 Spear, P. G. 1993. Entry of alphaherpesviruses into cells. Seminars Virol. 4, 167-180.   DOI
5 Studdert, M. J., T. Simpson, and B. Roizman. 1981. Differentiation of respiratory and abortigenic isolates of equine herpesvirus 1 by restriction endonucleases. Science 214, 562-564.   DOI
6 Studdert, M. J. 1996. Virus infections of equines. pp. 39-46, 1st ed., Elsevier, Amsterdam.
7 Sullivan, D. C., S. S. Atherton, J. Staczek, and D. J. O'Callaghan. 1984. Structure of the genome of equine herpesvirus type 3. Virology 132, 352-367.   DOI
8 Girard, A., A. S. Greig, and D. Mitchell. 1968. Virus associated with vulvitis and balanitis in the horse-a preliminary report. Can. J. Comp. Med. 32, 603-604.
9 Hartley, C. A., H. E. Drummer, and M. J. Studdert. 1999. The nucleotide sequence of the glycoprotein G homologue of equine herpesvirus 3 (EHV3) indicates EHV3 is a distinct equid alphaherpesvirus. Arch. Virol. 144, 2023-2033.   DOI
10 Jacob, R. J. 1986. Molecular pathogenesis of equine coital exanthema: Temperature-sensitive function(s) in cells infected with equine herpesviruses. Vet. Microbiol. 11, 221-237.   DOI
11 Jacob, R. J. and M. R. Steiner. 1986. Molecular pathogenesis of equine coital exanthema (ECE): Identification and expression of infected cell polypeptides at the restricted temperature during equine herpesvirus 3 infection. Vet. Microbiol. 18, 363-371.
12 Kamada, M. and M. J. Studdert. 1983. Analysis of small and large plaque variants of equine herpesvirus type 3 by restriction endonucleases. Arch. Virol. 77, 259-264.   DOI
13 Kobluk, C. N., T. R. Ames, and R. J. Geor. 1995. The horse diseases & clinical management. pp. 557-985, 1st ed., Saunders, Philadelphia.
14 Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 15, 227, 680-685.
15 Ostlund, E. N. 1993. The equine herpesvirus. Vet. Clin. North Am. Equine Pract. 9, 283-294.
16 Pascoe, R. R., P. B. Spradbrow, and T. J. Bagust. 1969. An equine genital infection resembling coital exanthema associated with a virus. Aust. Vet. J. 45, 166-170.   DOI
17 Reed, S. M. and W. M. Bayly. 1998. Equine internal medicine. pp. 545-546, 1st ed. Saunders, Philadelphia.
18 Cho, G. J., B. H. Kim, D. S. Lee, M. Y. Oh, and M. H. Ko. 1995. Studies on characters and immunogenicity of equine herpesvirus isolated from Korean horses. Korean J. Vet. Res. 35, 743-752.
19 Borchers, K. and J. Slater. 1993. A nested PCR for the detection and differentiation of EHV-1 and EHV-4. J. Virol. Methods 45, 331-336.   DOI
20 Bryans, J. T. and G. P. Allen. 1973. In vitro and in vivo studies of equine coital exanthema, pp. 322-336, In Bryans, J. T. and H. Gerber (eds.), Equine Infectious Diseases III. S. Karger, New York.
21 Colahan, P. T., A. M. Merritt, J. N. Moore, and I. G. Mayhew. 1999. Equine medicine and surgery. pp. 1071-1921, 5th eds., Mosby, Missouri.
22 Crandell, R. A. and E. R. Davis. 1985. Isolation of equine coital exanthema virus (equine herpesvirus 3) from the nostril of a foal. J. Am. Vet. Med. Assoc. 187, 503-504.
23 Fields, B. N., D. M. Knipe, R. M. Chanock, P. M. Howley, J. L. Melnick, T. P. Monath, B. Roizman, and S. E. Straus. 1995. Fundamental virology. pp. 1043-1107, 3rd eds., Lippincott-Raven, New York.
24 Fukushi, H., A. Tanaiguchi, K. Yasuda, T. Yanai, T. Masegi, T. Yamaguchi, and K. Hirai. 2000. A hamster model of equine herpesvirus 9 induced encephalitis. J. Neurovirol. 6, 314-319.   DOI