• Title/Summary/Keyword: 성 판별

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Development of specific SNP molecular marker from Thistle using DNA sequences of ITS region (엉겅퀴의 ITS 영역 염기서열 분석을 통한 특이적 SNP 분자마커의 개발)

  • Lee, Shin-Woo;Lee, Soo Jin;Kim, Yun-Hee
    • Journal of Plant Biotechnology
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    • v.45 no.2
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    • pp.102-109
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    • 2018
  • Thistle is a perennial plant that is widely used for medicinal purposes. Information on the genetic diversity of thistle populations are great important for their conservation and germ plasmic utilization. Although thistle is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish them from other similar species from different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from the nuclear ribosomal DNA internal transcribed spacer (ITS) regions of genomic sequences to identify distinct Korean-specific thistle species via an amplification refractory mutation system (ARMS)-PCR and high resolution melting (HRM) curve analyses. We performed molecular authentication of four different kinds of thistle species from different regions using DNA sequences in the ITS intergenic region. We also developed a quantitative PCR assay using species-specific ITS primers, which allowed us to estimate the ratio of Korean-specific thistle species using varying ratios of mixed genomic DNA templates from the two species. The SNP markers developed in this study are useful for rapidly identifying specific thistle species from different countries.

Development of molecular markers for the differentiation of Angelica gigas Jiri line by using ARMS-PCR analysis (세발당귀(Angelica gigas Jiri)의 판별을 위한 ARMS-PCR용 분자표지 개발)

  • Lee, Shin-Woo;Lee, Soo Jin;Han, Eun-Hee;Shin, Yong-Wook;Kim, Yun-Hee
    • Journal of Plant Biotechnology
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    • v.48 no.1
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    • pp.26-33
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    • 2021
  • Angelica is a widely used medicinal and perennial plant. Information on the genetic diversity of Angelica populations is essential for their conservation and germ plasmic utilization. Although Angelica is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish it from other similar species from different countries. This developed single nucleotide polymorphism (SNP) markers derived from nuclear ribosomal DNA internal transcribed spacer regions genomic sequences to identify distinct Korean-specific Angelica species via amplification refractory mutation system (ARMS)-PCR curve analyses. We performed molecular authentication of different kinds of Korean-specific Angelica species such as A. gigas Nakai and A. gigas Jiri using DNA sequences in the ITS intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific Angelica species from different countr.

Radar identification by scan period validation (스캔주기 유효성 판별에 의한 레이더 식별)

  • Kim, Gwan-Tae
    • Journal of Convergence for Information Technology
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    • v.11 no.11
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    • pp.17-22
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    • 2021
  • Radar signal analysis of electronic warfare is a technique for identifying a radar type by signal parameters(direction, radion frequency, pulse repetition interval, pulse width, scan period..) extracted from a received radar pulse. However as the modern radar and new threat environments is advanced, radar identification ambiguity arises in the process of identifying the types of radars. In this paper, we analyze the problems of the existing method and propose a new method. This technique determines the validity of the scan period by the difference in the arrival time of the radar pulse and the minimum number of scan period discrimination. Experiments proved that the scan cycle results are derived regardless of the RMS((Root Mean Square) of the input amplitude.

Development of an EEG Based Discriminant-Scale for Scientifically Gifted Students in Elementary School (초등학교 과학 영재아의 뇌파 기반 변별 척도 개발)

  • Kwon, Suk-Won;Kang, Min-Jung;Shin, Dong-Hoon;Kwon, Yong-Ju
    • Journal of Korean Elementary Science Education
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    • v.25 no.spc5
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    • pp.556-566
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    • 2007
  • The purpose of this study was to develop an electroencephalogram (EEG) based differential-scale for scientifically gifted students in elementary school. For this study, signals of EEG with 19 channels were recorded during the generation of our scientific hypothesis using 22 scientifically gifted students, and with 49 average students being used as the control group. IQ, TCT and knowledge generation (KG) as constructs of the scientifically gifted were administered for both the scientifically gifted and the normal, control group elementary students. A 'gifted' value was added to paper test scores of the IQ, TCT, and KG constructs in order to make a personal standardization score for the gifted students. As a dependent variable, the groups were divided by means of the standardization scores thus produced and as an autonomous variable, various EEG parameters were presented through linear analysis, nonlinear analysis, and interdependency measures of the EEG. Multiple linear regression analysis was applied successfully to explain the EEG parameters and to show the characteristics of the scientifically-gifted. The discrimination analysis was administered through the results of multiple linear regression of the EEG parameters thus produced. This study represents the foundation of the development of an EEG based discriminant-scale for scientifically gifted students in elementary school, because it will be able to faithfully discriminate between scientifically-gifted and average students. The results of this study indicates that most of the EEG parameters produced can contribute to predicting the characteristics of the scientifically-gifted in that they express the degree of mutual information and the coherence of mutuality. Accordingly, mutual connectivity which appears to originate in the brain seems to the core of discrimination.

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Molecular Sex Determination Using Sexual Dimorphisms between ZFX and ZFY Genes in Korean Hares(Lepus coreanus Thomas) (한국멧토끼 ZFX와 ZFY 유전자의 성별 이형성과 분자 성판별)

  • Han, Sang-Hyun;Cho, In-Cheol;Lee, Sung-Soo;Oh, Moon-You;Oh, Hong-Shik
    • Journal of Life Science
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    • v.17 no.3 s.83
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    • pp.402-406
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    • 2007
  • This study was performed to develop the molecular marker for sex determination of hare (Lepus coreanus) distributed in Korea which focused on sexual dimorphism between X and Y chromosomal homologous genes, zinc finger-X (ZFX) and -Y (ZFY). The intron 7 regions of ZFX and ZFY genes exhibited differential amplification patterns between male and female hares. The lengths of intron 7 region of ZFX and ZFY genes were 538 and 233-bp, respectively. Especially, the ZFX intron 7 contained a repetitive sequence identified as member of RNA-mediated transposable elements which was similar to CSINE2 commonly found in the rabbit genome. However, it was not present in intron 7 of ZFY gene. The molecular sex typing by polymerase chain reaction (PCR) was also carried out to determine the sex of hare based on difference in lengths between the intron 7 regions of ZFX and ZFY genes. All DNA samples tested had common band amplified from ZFX. However, the male hare DNAs had two distinct bands which amplified from ZFX and ZFY genes, respectively. The results from ZFX-ZFY PCR sex typing were identical to those from phenotypic investigation and from amplification patterns using male-specific sex determining region Y (SRY) gene as well. Finally, this study suggested that the sexual dimorphism between intron 7 regions of ZFX and ZFY could be useful genetic marker to determine sex of hare.

Brand Positioning Strategy of Franchise Family Restaurant (프랜차이즈 패밀리레스토랑의 브랜드 포지셔닝에 관한 연구)

  • An, Hye-Lim;Sohn, Jeong-Min;Choi, Ji-Mi;Kim, Hak-Seon
    • Culinary science and hospitality research
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    • v.21 no.4
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    • pp.339-348
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    • 2015
  • The purpose of this study is to suggest positioning strategy according to franchising family restaurant brands. Discriminant Analysis was utilized to suggest methodological framework that seeks to find their relative positions in the family restaurant brands. As a result, the results show that five factors (employee service, physical environment, marketing, accessibility, and food quality) were extracted from Exploratory Factor Analysis that contains twenty three questions related to service quality of family restaurant. Second, Discriminant Analysis revealed that three factors out of five including employee service, physical environment, and accessibility showed the highest discriminant power. Based on the three factors, positioning maps were made and the location of four brands were identified. Third, service quality, satisfaction, and willingness had a significant influence on revisit intention.

THE RETROSPECTIVE STUDY ON THE PROGNOSIS OF CLASS III MALOCCLUSION TREATMENTS (III급 부정교합의 치료후 예후에 관한 후향적 고찰)

  • Sung, Jae-Hyun;Kwon, Oh-Won;Kim, Sang-Doo
    • The korean journal of orthodontics
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    • v.28 no.2 s.67
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    • pp.175-187
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    • 1998
  • The purpose of this study was to predict the prognosis of class III malocclusion treatments. 25 patients selected for this study were devided into two groups by the stability of dentitional, skeletal and soft tissue profile improvement. One was stable group which consisted of 12 children and the other was relapse group with 13 children. Various measurements in initial lateral cephalogram were calculated and analyzed by t-test, correlation coefficient and discriminant analysis. The results of this study were summarized as follows 1. In both stable and relapse groups, there was not distinct difference in the antero-posterior skeletal relationship (P<0.05). 2. As the result of wits, hn to occlusal plane angle and occlusal plane to mandubular plane angle, the occlusal plane of relapse group was stepper than that of the stable group. 3. In correlation coefficient analysis, the overjet and the occlusal plane to mandibular plane angle showed significant correlations (p<0.001, p<0.01). 4. The discriminant function was obtained from three major influential measurements; overjet, AB to occlusal plane angle and articular angle, and this function could discriminate correctly in 88% of these samples.

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Digital Imaging Source Identification Using Sensor Pattern Noises (센서 패턴 잡음을 이용한 디지털 영상 획득 장치 판별)

  • Oh, Tae-Woo;Hyun, Dai-Kyung;Kim, Ki-Bom;Lee, Hae-Yeoun
    • KIPS Transactions on Software and Data Engineering
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    • v.4 no.12
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    • pp.561-570
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    • 2015
  • With the advance of IT technology, contents from digital multimedia devices and softwares are widely used and distributed. However, novice uses them for illegal purpose and hence there are needs for protecting contents and blocking illegal usage through multimedia forensics. In this paper, we present a forensic technique for identifying digital imaging source using sensor pattern noise. First, the way to acquire the sensor pattern noise which comes from the imperfection of photon detector against light is presented. Then, the way to identify the similarity of digital imaging sources is explained after estimating the sensor pattern noises from the reference images and the unknown image. For the performance analysis of the proposed technique, 10 devices including DSLR camera, compact camera, smartphone and camcorder are tested and quantitatively analyzed. Based on the results, the proposed technique can achieve the 99.6% identification accuracy.

Analysis on Effectiveness of Transit Mall by Visitor Perception (시민 만족도 분석을 통한 대중교통전용지구 도입 평가 연구)

  • JUNG, Hun Young;LEE, Sang Yong;LIM, Seong Beom
    • Journal of Korean Society of Transportation
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    • v.34 no.4
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    • pp.330-340
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    • 2016
  • The main objective of study was to analyze the effectiveness of transit mall. To this end, this study compared operational status of transit malls in operation (Daegu, Seoul and Busan). For the city of Busan where the latest transit mall became operational in 2015, a discriminant model was proposed to determine user satisfaction/dissatisfaction. The outcomes of comparative study showed that each city has different operational focus - Seoul has focus on traffic demand management, Busan has focus on walking environment, and Daegu has focus on accessibility to public transportation. Also, the discriminant model indicated that market sentiment, convenience of bus service, traffic accident risk, number of pedestrian, market user number have effects on user satisfaction. These results implied that, to improve user satisfaction, measures for market revitalization such as opening events and forming consultative groups need to be prepared and traffic conditions should also be improved through expansion of curfew time, linkage with subway station.