• Journal of the Korean Association of Geographic Information Studies
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• v.22 no.1
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• pp.78-92
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• 2019

This study aims to evaluate the efficiency in the provision of employment services for the middle-aged by using spatial analysis in GIS. Based on location information of employment service institutions, we find service areas of the institutions and calculate regional rates of duplication and exclusion in terms of spatial coverage of the employment services. Taking into account potential demand for employment services, the regions with high priority in the provision of the services are identified. Among the regions, those with high exclusion rate of the services are designated as the regions of insufficient service level. Results indicate that Namyangju-si is a representative region of insufficient employment service level. To improve efficiency in the provision of employment services, we suggest to relocate employment service institutions that have been located in a region of high duplication rate such as Siheung-si, Danwon-gu, Gangnam-gu, Songpa-gu into the locality of Namyangju-si.

• Korean Journal of Microbiology
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• v.54 no.4
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• pp.420-427
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• 2018

The orthogonal pair of Saccharomyces cerevisiae tyrosyl-tRNA synthetase (Sc YRS) and a variant of E. coli initiator tRNA, fMam $tRNA_{CUA}$ which recognizes the amber stop codon is an effective tool for site-specific incorporation of unnatural amino acids into the protein in E. coli. To evolve the amber suppression activity of the orthogonal pair, we generated a mutant library of Sc YRS by randomizing two amino acids at 320 and 321 which involve recognition of the first base of anticodon in fMam $tRNA_{CUA}$. Two positive clones are selected from the library screening with chloramphenicol resistance mediated by amber suppression. They showed growth resistance against high concentration of chloramphenicol and their $IC_{50}$ values were approximately 1.7~2.3 fold higher than the wild type YRS. In vivo amber suppression assay reveals that mutant YRS-3 (mYRS-3) clone containing amino acid substitutions of P320A and D321A showed 6.5-fold higher activity of amber suppression compared with the wild type. In addition, in vitro aminoacylation kinetics of mYRS-3 also showed approximately 7-fold higher activity than the wild type, and the enhancement was mainly due to the increase of tRNA binding affinity. These results demonstrate that optimization of anticodon recognition by engineered aminoacyl tRNA synthetase improves the efficiency of unnatural amino acid incorporation in response to nonsense codon.

• Korean Journal of Microbiology
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• v.55 no.2
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• pp.143-148
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• 2019

The synthesis of captopril as an important chiral drug in commerce needs expensive resolution process of racemic mixture. Microorganisms, producing a thermostable esterase that catalyzes the stereo-selective hydrolysis of methyl DL-${\beta}$-acetylthioisobutyrate (DL-ester) to D-${\beta}$-acetylthioisobutyric acid (DAT) were screened from soils. Among the strains tested, strain No CJ-317 and strain No CJ-187 with highest activity were selected as the best DAT producer. The newly isolated microorganisms were identified respectively, as Klebsiella pneumoniae and Pseudomonas putida. The cell activity of esterase from K. pneumoniae CJ-317 and P. putida CJ-187 were showed an optimal reaction activity at $75^{\circ}C$ and $60^{\circ}C$, respectively. Also the cell activity of K. pneumoniae CJ-317 was stable up to $80^{\circ}C$ for 1 h, while that of P. putida CJ-187 was not over $60^{\circ}C$. By varying the concentration of DAT in the reaction mixture, the cell activity of P. putida CJ-187 showed about 55% and 80% of product inhibition in the presence of 2.5% (w/v) and 5.0% of DAT respectively. K. pneumoniae CJ-317 had less product inhibition than P. putida CJ-187 by about 35% and 44% at the same concentrations respectively. The esterase of newly isolated K. pneumoniae CJ-317 could be useful for the stereo-selective hydrolysis of DL-ester to DAT.

• Journal of the Korean Chemical Society
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• v.64 no.6
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• pp.379-388
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• 2020

The purpose of this study are to develop the TEP activity for learning experimental apparatus at elementary school and to test the effects of the TEP activity. This study consists of two steps. First through literature research on the difficulties and needs of experimental apparatus education developed the form that how to educate the experimental apparatus at elementary school. Second, applied the TEP activity and figured out the effects as two aspect(knowledge about experimental apparatus and actual using skill during lesson). This worksheet was applied to 3rd grade students in elementary school about 4 experimental apparatuses(Beaker, Electronic scale, Glass rod, Spatula). The results of this study are as follows: There is no specific time to teach what is and how to use experimental apparatus by regular curriculum. So many students and teachers need method and time to learn them. Also they want to lots of opportunities to use them. With that needs given previously, TEP activity developed by 3 steps. 1. Trigger interest 2. Explore experimental apparatus: learned knowledges about experimental apparatus focused on appearance(name, purpose, directions for use, precautions) 3. Practice experimental apparatus: actual using time to acquire skills. After that did the survey of knowledge and observation of students' behavior during usual class to confirm the effects. According to the results, TEP activity helped the students to improve there awareness of the experimental apparatus and actual using skills.

• Journal of the Korea Convergence Society
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• v.13 no.4
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• pp.339-348
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• 2022

As the Chinese franchise industry grows rapidly, efforts to find a source of competitive advantage continue to be made. Establishing a knowledge transfer system is important in the franchise industry as a source of competitive advantage. However, research in China was insufficient. This study is to explore factors and consequential factors for establishing a knowledge transfer system in the franchise industry. As a result of this study, first, it was found that not only human assets but also system assets are important for the management of knowledge transfer systems in China. Therefore, investment in system assets must proceed first. In addition, it could be seen that the management of the knowledge transfer system, human assets, and system assets affect the satisfaction of the franchise's transaction relationship and consequently improve franchise performance.

• The Journal of the Korea Contents Association
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• v.22 no.7
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• pp.486-497
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• 2022

The aim of this study was to subdivide and present the units and the standards of knowledge integration in creating the students' integrated knowledge from content knowledge in college writing classes. For these, it analyzed three typical writing textbooks being used in colleges and examined the ways of presentation on forming integrated knowledge by text qualitative analysis methods. The analysis procedure and the presentation followed Creswell's spiral analysis model It is a method model which repeats the procedure from material collection and analysis to presentation circularly. This examination illustrates three dimensions of the units in forming content knowledge. Also, it suggested those should be all treated for the more systematic education: the units of the whole text, the paragraphs, and the sentences. In the next chapter, the standards and contents of knowledge integration were suggested in each process. For the process of knowledge selection, the suitability and the contradictoriness between the text materials and author's thesis were proposed as the standards and contents. For the process of organization and integration, the corresponsive integration, contradictive integration, background integration, synthetic integration were suggested. Finally the procedure knowledge such as correct expression and spelling, source indication were shown for the process of expression and citation. Furthermore, it showed, in terms of expression, the process of paraphrasing frequently practiced in writing textbooks needs to be exercised in the three dimensions including summarization, connection, and interpretation(or transformation). This result, however, calls for the further study about the subdividing processes to enhance the adequateness to writing textbooks in the level of universities and for a more refined syllabus on the systematic knowledge integration. Accordingly, it suggested the tasks mentioned above for further study.

• Journal of Digital Convergence
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• v.20 no.2
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• pp.241-250
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• 2022

Due to the COVID-19 pandemic, with increased 'untact' services and with unstable household economy, the bike insurance fraud is expected to surge. Moreover, the fraud methodology gets complicated. However, the fraud detection model for bike insurance is absent. we deal with the issue of skewed class distribution and reflect the criterion of fraud detection expert. We utilize a balanced random-forest algorithm to develop an efficient bike insurance fraud detection model. As a result, while the predictive performance of balanced random-forest model is superior than it of non-balanced model. There is no significant difference between the variables used by the experts and the confirmatory models. The important variables to detect frauds are turned out to be age and gender of driver, correspondence between insured and driver, the amount of self-repairing claim, and the amount of bodily injury liability.

• 한국체육학회지인문사회과학편
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• v.58 no.3
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• pp.241-260
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• 2019

The purpose of this study is to explore effective and practical career plans reflecting demand of the field, in regard to education for university students who major in physical education, as the importance and necessity of career education at a university level are increasing. Systematic literature review (PRISMA) methodology was used for this study. From 38 pieces of literature that were finally selected for this study, research results were put together from three perspectives as follows: 1. 'Factors Related to Students' Decision-Making for Career' which should be considered in career education for physical education majors; 2. Problems of Career Education in the Physical Education Curriculum, and; 3. Improvement Plans. First, it was found that students' decision-making for career is affected by individuals' personal internal factors (i.e., satisfaction with major, self-efficacy, and career barrier) and external factors (i.e., counselling history on career paths, career conditions, and the status of related jobs). Second, the state of career education for physical education majors was found to be insufficient in terms of a lack of career education in the curriculum, a lack of support from major professors, and a lack of support for field experience. Third, improvement plans were suggested from the following perspectives: development of systematic career education curriculum (i.e., fostering leadership, reorganization of curriculum focused on career specialty, and operation of systematic curriculum); provision of an adequate environment for career education (i.e., establishment of infrastructure that links the university to society and collection of information on career paths for physical education majors). Moreover, the necessity to develop career education curriculum in connection with demand of students regarding career, the lack of systematic information on career paths of physical education majors, and the lack of researches on career education for physical education majors were discussed in this study.

• Journal of the Korean Society for Library and Information Science
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• v.56 no.2
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• pp.5-33
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• 2022

In this study, the direction and contents of public education to develop competencies that are in line with the 2022 revised curriculum and the paradigm of future education were sought, and a plan for reading education was prepared. A creative and cooperative problem-solving method and process as not only information literacy ability to read, select, and reconstruct information but also transformative competency to respond to uncertain future and diversified situations are important amid the complex, pluralistic and rapid development of information and communication technology It was intended to give an experience of exploring and communicating through reading in order to explore and derive it. Analyze the general outline and syllabus of the curriculum, international education project definitions and indicators, and organize academic theories and research to set the direction and goal of high school reading education, and organize creative and convergence class strategies and reading activities A library reading class model was developed. Accordingly, the class model was revised by applying the development research method, and the final model was developed by supplementing it through field application evaluation. In order to achieve the research purpose end, a two-round Delphi survey was conducted on 10 reading education and curriculum experts. The model modified through the Delphi survey was developed in the final school library convergence reading class model by demonstrating the class in the educational field and supplementing it through application evaluation.

• Economic and Environmental Geology
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• v.56 no.2
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• pp.201-215
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• 2023

In the stone-lined tombs from Cheongyang Rokpyeong-ri site, several stone-lined tombs corresponding to the Three Kingdoms Period, Unified Silla Period and Goryeo Dynasty were excavated. In this study, the stone properties of petrological, mineralogical and geochemical characteristics from 15 stone-lined tombs were analyzed, the rock species and occupation rate were reviewed, also the provenance area was interpreted. There are five types of rocks used as members of the tombs, including black sandstone, greywacke, slate, conglomerate and gneiss. As a result of magnetic susceptibilities, the rocks of the tombs and presumed provenance are all shown in the same range, and the similarity between the tombs and the source area is high in geochemical behavior characteristics. The stone properties of the tombs are the same as the presumed provenance rocks and is distributed within a 500m around the bedrock of the tombs in the excavation site, so it seems that self-sufficiency was possible on the site without a special procurement system. These stones are interpreted to have been constructed through some trimming without any special technique, but some stone tombs have been confirmed to used homogeneous black sandstone at the bottom of the tombs, so it is believed that there were artificial selection of stones.