• The Korean Journal of Ecology
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• v.25 no.3 s.107
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• pp.153-161
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• 2002

This study was carried out to classify forest vegetation of the Mt. Naeyeon with phytosociological analysis of ZM school and to explain ordination of communities with CCA. The research sites were located between the northern part of Pohang-si area and the southern part of Yeongdeok-gun area. The 70 plots consisted of 253 species were investigated. The forest vegetation was classified into Quercus mongolica community, Q. variabilis community, Pinus densiflora community, Carpinus laxiflora community and Zelkova serrata community. Q. mongolica community was divided into 2 groups such as Rhododendron schlippenbachii group and Euonymus oxyphyllus group and Q. variabilis community was divided into 3 groups such as Syneilesis palmata group, Lespedeza$\times$tomentilia group and Tilia mandshurica group, and Z. serrata community was divided into 2 groups such as Carpinus cordata group and Diospyros lotus group. According to the results of CCA ordination, Q. mongolica community showed high positive correlation to altitude and topography, whereas it showed negative correlation to bare rock. But Z. serrata community showed the opposite tendency to Q. mongolica community, Altitude was considered as the most important factor among 5 environmental variables in the correlation with axes.

• Korean Journal of Food Science and Technology
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• v.52 no.1
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• pp.40-45
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• 2020

Over 500 genetically modified organisms (GMOs) have been developed since 1996, of which nearly 44% have glufosinate herbicide-tolerant traits. Identification of specific markers that can be used to identify herbicide-tolerant traits is challenging as the DNA sequences of the gene(s) of a trait are highly variable depending on the origin of the gene(s), plant species, and developers. To develop specific PCR marker(s) for the detection of the glufosinate-tolerance trait, DNA sequences of several pat or bar genes were compared and a diverse combination of PCR primer sets were examined using certified reference materials or transgenic plants. Based on both the qualitative and quantitative PCR tests, a primer set specific for pat and non-specific for bar was developed. Additionally, a set of markers that can detect both pat and bar was developed, and the quantitative PCR data indicated that the primer pairs were sensitive enough to detect 0.1% of the mixed seed content rate.

• Journal of Environmental Science International
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• v.12 no.12
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• pp.1207-1213
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• 2003

Osancheon creek is located in the southwest of the middle area in Korea. Weed community around the Osancheon creek, where various disturbances exert a bad influence directly on its community by human work or nature, was surveyed phytosocialogically. Our surveys have been accomplished from June, 2003 to September, 2003. Weed communities formed around the Osancheon creek were divided into several patterns and analysed. They have been divided into 8 communities. Community A: Humulus japonica community, B: Phragmites communiscommunity, C: Persicaria thunbergii community, D: Zi=ania latifolia community, E: Miscanthus saccharilflorus community, F: Echinochica crusgalli community, G: Typha orientalis community, H: Sataria viridis community. The flora surveyed in these communities was constituted of 19 families, 36 genera, 4 varieties, and 40 species. Wild plants such as Artemisia princeps var. orientalis Erigeron annuus, Bidens frondosa, Aeschynomene indica and Oenothera odorata were mostly light loving plants and higher resistant plants against the disturbance. Our result from the ranking all surveyed areas by the Bray-Curtis ordination method was very similar to the results from phytosocialogical table work.

• The Korean Journal of Mycology
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• v.44 no.2
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• pp.103-107
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• 2016

To establish a rapid and accurate detection of Phytophthora pinifolia, which is a quarantine pathogenic fungus in Korea, a species-specific primer was developed based on the ras-related protein (Ypt1) gene. Species-specific primer based on the DNA sequences of Ypt1 gene amplified 193 bp polymerase chain reaction (PCR) product for P. pinifolia. The primer pair yielded the predicted PCR product size exactly in testing with target pathogen DNAs, but not from the other 10 species of Phytophthora and 14 species of other phytopathogenic fungi. The primer pair also showed only the species-specific amplification curve on realtime PCR on target pathogen DNA. The detection sensitivity of real time PCR using species-specific primer pair was 10 to 100 times higher than conventional PCR, with 1 to $10pg/{\mu}L$.

• Korean Journal of Ecology and Environment
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• v.53 no.3
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• pp.265-274
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• 2020

Silver croaker (Pennahia argentata) is a turbulent species that is widely distributed worldwide and is mainly found in the bottom of the ocean. In the study, we characterized the cytochrome c oxidase subunit I (COI) gene of the mitochondrial DNA (mtDNA) on P. argentata inhabiting Gwangyang Bay and analyzed the phylogenetic location of marine fish species. As a result of multiple arrangement of 605 bp COI sequences, high homology of mtDNA nucleotide sequences was confirmed in the silver croakers from Gwangyang Bay (98~100%). However, the nucleotide variation was different according to the catching points of the inland and the open seas of Gwangyang Bay. The nucleotide sequence variation in COI was high in P. argentata from the open seas of Gwangyang Bay (43.2~70.3%). Furthermore, the phylogenetic analysis of 13 fish showed that P. argentata from Gwangyang Bay were grouped into one clade with P. argentata reported in Taiwan, and the evolutionary distance was 0.036. In addition, it was identified that the evolutionary distance was close to that of fish belonging to the Mi-iuy croaker (Miichthys miiuy) and the Big-head pennah croaker (Pennahia Macrocephalus) (0.041~0.048). The result of these studies will be used as the key genetic information for fisheries resources monitoring and species diversity management according to the coastal environment.

• Korean Journal of Ecology and Environment
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• v.54 no.3
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• pp.240-246
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• 2021

The Chironomidae is a benthic macroinvertebrate commonly found in freshwater ecosystems, along with Ephemeroptera and Trichoptera, which can be used for environmental health assessments. There are approximately 15,000 species of Chironomidae worldwide, but there are limited studies on species identification of domestic Chironomidae larvae. In the present study, we carried out species classification of the Chironomidae larvae that found in Jeju's tap water purification plants using morphological characteristics and genetic identification based on cytochrome c oxidase subunit I (COI) gene of the mitochondrial DNA. Body shape, mentum, antenna, mandible in the head capsule, and claws were observed in the larvae for morphological classification. Analysis of 17 larvae collected from faucets and fire hydrants of domestic tap water purification plants revealed the presence of two species, including 14 Orthocladius tamarutilus and 3 Paratrichocladius tammaater. These results will aid the use of the criteria information about species classification of the Chironomidae for water quality management in water purification plants and diversity monitoring of freshwater environments.

• Proceedings of the Korean Information Science Society Conference
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• 2003.04c
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• pp.461-463
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• 2003

휴먼지놈프로젝트이후 컴퓨터를 이용한 연구는 점차로 활발하게 진행되고 있는데 그 중 단백질의 기능예측과 관련하여 보다 많은 연구가 이루어지고 있다. 단백질의 기능예측을 위해서는 3차원 구조정보가 많이 이용된다. 3차원 구조를 형성하는 것은 주로 아미노산 서열이나 1차, 2차 구조 정보가 보다 구체적인 단백질 구조예측을 위해 이용되고 있다. 전세계적으로 다량의 단백질 구조정보 및 예측을 위한 방법들이 소개되고 있지만 각 자원들마다 저장, 관리 형식이 다를 뿐만 아니라, 정보를 이용하는 방법도 어렵다. 본 논문에서는 다양하게 존재하는 단백질 구조 데이터베이스 자원들을 에이전트화여 통합성과 재사용성을 지향하였고, 에이전트시티 네트워크에 연결함으로써 개방성과 확장성, 분산성을 높이도록 하였다.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2004.10a
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• pp.505-520
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• 2004

정부는 중소기업의 업무성과(업무처리의 신속성과 능력 향상, 정확한 자료관리, 고객 대응 능력 향상, 의사결정의 신속성)를 높이고자 하는 목표를 가지고 다양한 정보화사업을 시행하고 있다. 본 연구는 이러한 목적으로 2001년부터 산업자원부가 지원한 3만개 중소기업의 ERP(Enterprise Resource Planning) 시스템의 도입에 따른 업무성과에 영향을 미치는 요인들을 파악하고자 한다. 이를 위하여 설문조사를 실시하였으며, 총 1022개의 설문응답을 회수하였다. 설문의 업무성과 응답이 5점 서열척도임을 고려하여 이에 적합한 ordered probit 회귀분석을 실시하였다. 분석 결과는 모든 종속변수들과 유의한 관련성을 가지는 독립 변수는 사용용이성, 시스템 안정성, 정보의 정확성, 그리고 정보의 다양성으로 나타났다.

• Proceedings of the Korean Information Science Society Conference
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• 2001.10a
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• pp.67-69
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• 2001

Human Genome Project의 완성을 전후로 이루어진 생물학적 분석도구의 발달과 서열 데이터베이스의 축적으로 단백질 분석은 괄목할 만한 성장을 하였다. 단백질 분석에 사용되는 가장 중요한 단백질 식별(Identification)을 위한 도구들은 많이 개발되어 왔으나 기존의 도구들은 파일 기반으로 폭발적으로 증가하는 단백질 데이터를 효율적으로 관리하고 심험자들에게 빠르고 정확한 검색결과를 제공하는데 한계를 보여주고 있다. 우리는 SWISS-PROT flatfile을 분석하여 관계형 데이터베이스고 구축하고, 단백질 식별에서 가장 많이 사용하고 있는 ‘질량분석 후 데이터베이스 검색’방법을 사용하는 시스템을 DBMS 기반으로 설계하였으며, 다양한 실험조건과 절차에 의해 얻은 단백질 조각의 질량 값과 이론적인 계산에 의해 얻은 값을 비교하여 실험에 쓰인 단백질 조각과 일치하는 것을 단백질 데이터베이터베이스로부터 검색할 수 있는 도구를 설계하고 구현하였다.

• Tuberculosis and Respiratory Diseases
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• v.49 no.5
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• pp.546-557
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• 2000

Background : Oligonucleotide chip technology has proven to be a very useful tool in the rapid diagnosis of infectious disease. Rifampin resistance is considered as a useful marker of multidrug-resistance in tuberculosis. Mutations in the rpoB gene coding $\beta$ subunit of RNA polymerase represent the main mechanism of rifampin resistance. The purpose of this study was to develop a diagnosis kit using oligonucleotide chip for the rapid and accurate detection of rifampin-resistance in Mycobacterium tuberculosis. Method : The sequence specific probes for mutations in the rpoB gene were designed and spotted onto the glass slide, oligonucleotide chip. 38 clinical isolates of Mycobacterium were tested. A part of rpoB was amplified, labelled, and hybridized on the oligonucleotide chip with probes. Results were analyzed with a laser scanner. Direct sequencing was done to verify the results. Result : The low-density oligonucleotide chip design어 to determine the specific mutations in the rpoB gene of M. tuberculosis accurately detected rifampin resistance associated with mutations in 28 clinical isolates. Mutations at codons 531, 526, and 513 were confirmed by direct sequencing analysis. Conclusion : Mutant detection using oligonucleotide chip technology is a reliable and useful diagnostic tool for the detection of multidrug-resistance in M. tuberculosis.