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http://dx.doi.org/10.4489/KJM.2016.44.2.103

Species-specific Marker of Phytophthora pinifolia for Plant Quarantine in Korea  

Kim, Narae (Department of Applied Biology, Chungnam National University)
Choi, You Ri (Department of Applied Biology, Chungnam National University)
Seo, Mun Won (Department of Applied Biology, Chungnam National University)
Song, Jeong Young (Department of Applied Biology, Chungnam National University)
Kim, Hong Gi (Department of Applied Biology, Chungnam National University)
Publication Information
The Korean Journal of Mycology / v.44, no.2, 2016 , pp. 103-107 More about this Journal
Abstract
To establish a rapid and accurate detection of Phytophthora pinifolia, which is a quarantine pathogenic fungus in Korea, a species-specific primer was developed based on the ras-related protein (Ypt1) gene. Species-specific primer based on the DNA sequences of Ypt1 gene amplified 193 bp polymerase chain reaction (PCR) product for P. pinifolia. The primer pair yielded the predicted PCR product size exactly in testing with target pathogen DNAs, but not from the other 10 species of Phytophthora and 14 species of other phytopathogenic fungi. The primer pair also showed only the species-specific amplification curve on realtime PCR on target pathogen DNA. The detection sensitivity of real time PCR using species-specific primer pair was 10 to 100 times higher than conventional PCR, with 1 to $10pg/{\mu}L$.
Keywords
Detection; Phytophthora pinifolia; Quarantine measure; Species-specific primers;
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