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http://dx.doi.org/10.9721/KJFST.2020.52.1.40

Development of glufosinate-tolerant GMO detection markers for food safety management  

Song, Minji (National Institute of Agricultural Sciences, Rural Development Administration)
Qin, Yang (National Institute of Agricultural Sciences, Rural Development Administration)
Cho, Younsung (National Institute of Agricultural Sciences, Rural Development Administration)
Park, TaeSung (National Institute of Agricultural Sciences, Rural Development Administration)
Lim, Myung-Ho (National Institute of Agricultural Sciences, Rural Development Administration)
Publication Information
Korean Journal of Food Science and Technology / v.52, no.1, 2020 , pp. 40-45 More about this Journal
Abstract
Over 500 genetically modified organisms (GMOs) have been developed since 1996, of which nearly 44% have glufosinate herbicide-tolerant traits. Identification of specific markers that can be used to identify herbicide-tolerant traits is challenging as the DNA sequences of the gene(s) of a trait are highly variable depending on the origin of the gene(s), plant species, and developers. To develop specific PCR marker(s) for the detection of the glufosinate-tolerance trait, DNA sequences of several pat or bar genes were compared and a diverse combination of PCR primer sets were examined using certified reference materials or transgenic plants. Based on both the qualitative and quantitative PCR tests, a primer set specific for pat and non-specific for bar was developed. Additionally, a set of markers that can detect both pat and bar was developed, and the quantitative PCR data indicated that the primer pairs were sensitive enough to detect 0.1% of the mixed seed content rate.
Keywords
herbicide-tolerant GM crops; GMOs detection markers; pat; bar; food safety;
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