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http://dx.doi.org/10.11614/KSL.2021.54.3.240

Morphological and Genetic Species Identification in the Chironomidae Larvae Found in Tap Water Purification Plants in Jeju  

Kwak, Ihn-Sil (Fisheries Science Institute, Chonnam National University)
Park, Jae-Won (Department of Ocean Integrated Science, Chonnam National University)
Kim, Won-Seok (Department of Ocean Integrated Science, Chonnam National University)
Park, Kiyun (Fisheries Science Institute, Chonnam National University)
Publication Information
Korean Journal of Ecology and Environment / v.54, no.3, 2021 , pp. 240-246 More about this Journal
Abstract
The Chironomidae is a benthic macroinvertebrate commonly found in freshwater ecosystems, along with Ephemeroptera and Trichoptera, which can be used for environmental health assessments. There are approximately 15,000 species of Chironomidae worldwide, but there are limited studies on species identification of domestic Chironomidae larvae. In the present study, we carried out species classification of the Chironomidae larvae that found in Jeju's tap water purification plants using morphological characteristics and genetic identification based on cytochrome c oxidase subunit I (COI) gene of the mitochondrial DNA. Body shape, mentum, antenna, mandible in the head capsule, and claws were observed in the larvae for morphological classification. Analysis of 17 larvae collected from faucets and fire hydrants of domestic tap water purification plants revealed the presence of two species, including 14 Orthocladius tamarutilus and 3 Paratrichocladius tammaater. These results will aid the use of the criteria information about species classification of the Chironomidae for water quality management in water purification plants and diversity monitoring of freshwater environments.
Keywords
Chironomidae; tap water purification plant; morphological classification; mitochondrial DNA; genetic species identification;
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