• Journal of Mushroom
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• v.4 no.3
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• pp.122-127
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• 2006

This study was carried out to investigate the mycelial growth and density of A. auricula treated four kinds of substances L-citrulline, L-carnitine, Senna, Garcinia cambogia made from 10, 100, 500, 1000, 1500, 2000 times solutions respectively. Senna all treatments made from 10, 100, 500, 1000, 1500, 2000 times solutions are available for mycelial growth and density than control. Senna treatment made from 100 times solution of them is the best. L-citrulline treatment made from 500 times solution grew more much 7.86mm for 15 days than control, also it was optimal. L-carnitine treatment made from 100 times solution grew more much 3.4mm for 15 days than control, also it was optimal. Garcinia cambogia all treatments made from 10, 100, 500, 1000, 1500, 2000 times solutions were not available for mycelial growth and density than control. But Garcinia cambogia treatment made from 1000 times solution of them was similar to control. Accordingly, these solutions like that were effective to mycelial growth and density than control.

• Journal of Life Science
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• v.8 no.5
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• pp.520-525
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• 1998

Effects of plant growth regulators and anti-oxidants for rapid multiplication of Cymbidium kanran were investigated. The best gelling agent was 2.5 g/1 gelrite which needed less quantity (about 28%) and half price than 9 g/1 chemi-cal agar. Undefined edible agar was only a little bit worse than chemical agar in growth, but the price was half as much as the latter. The higher concentration of BA and NAA, the deeper browning of medium that prevented from performing its functions of plant growth regulators. Polyvinylpyrrolidone (M.W. 40,000) was the most effective anti-oxidant other than ascorbic acid, aspartic acid, and rutin in protecting the browning of medium, enhancing the effect of plant growth regulators, and thus prolonging the subculture cycle. Vigorous seedlings were obtained by 0.1∼1.0 mg/1 BA,0.1 mg/1 NAA and 1 g/1 polyvinylpyrrolidone treatments. Therefore, the best result for growth and econo-mic aspects in rhizome culture of Cymbidium kanran were obtained by using MS basal medium with 2.5 g/1 gelrite, 1 g/1 polyvinylpyrrolidone, 0.1∼1.0 mg/1 BA and 0.1 mg/1 NAA.

• Korean Journal Plant Pathology
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• v.1 no.1
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• pp.56-60
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• 1985

The present researches were carried out to examine the favorable nutritional conditions for mycelial growth of Cercospora kikuchii, and inhibition of bacterial growth by the fungus at various cultural conditions. As the nitrogen source, the fungus grew better on yeast-extract than the other sources of nitrogen tested. Sucrose, glucose, maltose and fructose were good sources of carbon for mycelial growth of the fungus. Two isolates of C. kikuchii, wild types A and D, inhibited the growth of bacteria on the medium containing yeast-extract or peptone as the nitrogen source, and sucrose, glucose, maltose or fructose as the carbon source, at pH 5.0. However, the albino mutant showed very little inhibitory effect under the same conditions.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.1
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• pp.7-13
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• 2004

Mung beans (Vigna radiata L.) were used to investigate the roles of strontium in hypocolyl elongation under IAA regime during the germination. After imbibition in a medium with or without IAA, $Sr^{2+}$ stimulated IAA oxidation. Three to five fold increasing in IAA oxidase activity seems to be direct evidence of growth inhibition through $Sr^{2+}$. Furthermore, the accumulation of spermidinc and spermine by $Sr^{2+}$ in the range of 1 to 10 mM was observed. Spermidine levels were 2 to 3 fold higher than in control seedling grown without strontium. The increase in polyamine levels was observed on a g fresh weight basis. In conclusion, it was demonstrated that the inhibitory action of $Sr^{2+}$ is closely related with the IAA oxidation and polyamine biosynthesis.

• The Korean Journal of Ecology
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• v.28 no.3
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• pp.135-139
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• 2005

This study was carried out to find the allelopathic effect of volatile materials released from Eupatorium rugosum. The GC method was employed for analysis of volatile materials from E. rugosum and 49 chemical substances were identified such as $\beta$-caryophyllene, $\alpha$-terpinenol, chamazulene, bornyl acetate, $\alpha$-pinene, etc. including unidentified three chemicals. Germination test in Phaseolus radiatus was done to find the inhibition effect of volatile materials using some chemicals which were proved to be important component or much amounts ones in E. rugosum. It was strongly inhibited by linalool and terpinen-4-ol. Seedling elongation and radicle growth of that were proportionally inhibited by the concentration of the essential oil, especially $\alpha$-pinene and bornyl acetate. Biomass of receptor plant was slightly decreased more than 58 ${\mu}l$ of the extract in case of $\alpha$-pinene, while it was decreased more than 19 ${\mu}l$ of that in bornyl acetate but it was shown non-significant. From the above results, it was found that volatile materials from E. rugosum showed a allelopathic effect and also $\alpha$-pinene, bornyl acetate, linalool and terpinen-4-ol used in bioassay were some of major allelochemicals in germination inhibition and especially linalool and terpinen-4-ol are prominent effect on growth inhibition of other plant.

• Journal of Korean Society of Forest Science
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• v.28 no.1
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• pp.21-30
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• 1975

The purpose of this paper is to elucidate physiologically the cause of the hastening germination of dormant Taxus cuspidata seeds by stratification. During the stratification the exchange of chemical substances such as sugar, protein, starch and fat were observed, and growth promoting and inhibiting substances were extracted and seperated from seeds by the conventional chromatographic method with coleoptile straight-growth test. An intensive investigation was made on the balance between the promoters and inhibitors. consequently, it was confirmed that germination of seeds was accelerated with exchange of chemical substances by stratification. The results obtained may be summarized as follows: 1. During the stratification growth promoters were increased and growth inhibitors were decreased rapidly in the endosperm of seeds. Thus, it was presumed that hastening germination was controlled by balace between the promoters and inhibitors from November to next March after a year's stratification. On the other hand growth promoters were almost constant and growth inhibitors were decreased rapidly in the seed coats, and it was presumed that hastening germination was influenced by exchange of inhibitors more than by that of promoters. 2. As a results of germination test of lettuce seeds, it was generalized that hastening germination was controlled by a decreased amount of growth inhibitors more than by an increased amount of promoters. 3. During the stratification sugar and crude protein contents were increased gradully with moisture content, while starch and crude fat were decreased in endosperm of seeds. So it was assumed that the exchange of these chemical substances was closely related to the germination of seeds.

• Journal of Environmental Science International
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• v.5 no.6
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• pp.813-826
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• 1996

The toxic effects of aluminium (Al) on growth, chlorophyll content, $\delta-aminolevulinic$ acid dehydratase (ALAD) activity and anatomy of root and shoot were investigated in 7-day-old azuki bean (Vigna angularis) seedlings. Significant depressions in root elongation was observed in the low concentrations of Al (50, 100 $\muM)$ and increasing Al concentrations caused a sharp decline of root and shoot growth. The degree of inhibition was dependent upon Al supply. Exposure to 50 $\muM$ Al or more inhibited root elongation within 1 day. In the 50 $\muM$ Al treatments, a recovery of root growth was seen after 7 days exposure. In contrast, lateral root initials was little affected by Al exposure. Al toxicity symptoms and growth responses were more well developed in the roots than in the shoots. Analysis of Al localization in root cells by hematoxylin stAlning showed that Al entered root apices and accumulated in the epidermal and cortical cells immeadiately below the epidermis. There was a good positive correlation between the level of chlorophyll and ALAD activity. Increasing Al concentrations caused a decrease in total chlorophyll contents, accompanied by proportional changes in ALAD activity, suggesting a cootr-dinated reduction of a photosynthetic machinery. Al exerted specific influence on the morphology of root ann shoot. At higher concentrations of Al the roots induced drastic anatomical changes. The epidermal cells were disorganized or destructed while the cortical cells exhibited distortion of cell shape and/or disintegration. The diameter of root and transectional area of cortical cells decreased considerably with Al treatment. In the shoot Al also enhanced reduction of diameter of shoot and cell size. Gross anatomy of leaves treated with Al did not differ significantly from the controls, except for fewer and smaller chloroplast. Our results indicate that toxic effect of Al appear to be manifested primarily in roots and secondarily on shoots, and changes in root morphology are related to changes in the root growth patterns. Results are further discussed in re181ion to the findings in other plant species, and it is concluded that Al causes morphological, structural and, presumably, functional damage to the roots of the species investigated.

• Korean Journal of Organic Agriculture
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• v.22 no.1
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• pp.129-145
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• 2014

To evaluate the potential of Hypochoeris radicata L. for the development of environment-friendly organic materials, its growth characteristics, allelopathic effects and antifungal activity was investigated. The growth characteristics of H. radicata L. was investigated by measuring comparative number of shoot and flower, and the diameters of clones in mowing areas and non-mowing areas, respectively. As a result, the number of shoot and flower, and diameters of clones of H. radicata L. grown in mowing areas were much higher than those grown in non-mowing areas. Water extracts of H. radicata L. inhibited seed germination, and shoot, root and root hair growth of 14 test plants including Trifolium pratense, Festuca myuros, Bidens bipinnata and finally reduced heir biomass remarkably. The inhibitory effect of the extract was different depending on the kind and the part of tested plants. The extracts showed high antifungal activity against Pythium spp. and Phytophthora However, it showed comparably less antifungal activity against Rhizoctonia solani than Pythium spp. and Phytophthora. In conclusion, cutting H. radicata L. resulted increase of its vegetative and seed propagation and helped it to form large colony. Also it had an effect on growth of microbes and germination and growth of other plants. Therefore H. radicata L. holds the competitive dominant position in plant ecosystem in Jeju Island and it can be used as candidate of environment-friendly organic materials.

• Journal of Life Science
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• v.24 no.12
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• pp.1364-1370
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• 2014

Ascorbic acid (AA) is a multifunctional metabolite in plants that is essential for plant development and growth. We examined the effect of AA, an antioxidant, on the gravitropic response of primary roots in maize. The application of $10^{-3}$ M AA to the elongation zone did not affect the gravitropic response and slightly inhibited the root growth. However, treatment with both $10^{-5}$ M and $10^{-3}$ M AA at the root tip increased the gravitropic response and inhibited root growth. Differences in indole-3- acetic acid (IAA) activity between the upper and lower hemispheres of the root resulted in differential elongation along the horizontal root. Roots are extremely sensitive to IAA, and increasing the amount of IAA in the lower hemisphere of the root inhibited elongation. Therefore, we examined the effect of IAA in the presence of AA. The inhibitory effect of AA on the gravitropic response was greater in combination with IAA. To understand the role of AA in the regulation of root growth and the gravitropic response, we measured ethylene production in the presence of AA in the primary roots of maize. AA stimulated ethylene production via the activation of the 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene, which regulates the conversion of ACC to ethylene. These results suggest that AA alters the gravitropic response of maize roots through modification of the action of ethylene.

• Korean Journal of Environment and Ecology
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• v.6 no.1
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• pp.9-11
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• 1992

To examine aluminum toxicity on woody plants, Forsythia koreana cuttings were grown in the aluminum solution and ground water(pH 6.75). Aluminum solution were prepared 1.0, 2.5 and 5.0mM aluminum potassium sulfate, dilulted with ground water. Shoot growth, leaf number, leaf injury and leaf chlorophyll content were measured and compared among the treatments. In all growth-related characters(shoot growth. leaf number, leaf injury and leaf chlorophyll content), differences among the treatments were highly significant. Forsythia koreana cuttings were severely stressed in aluminum solution more than 1.0mM concentration.