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http://dx.doi.org/10.5352/JLS.2014.24.12.1364

Effect of Ascorbic Acid on the Gravitropic Response of Primary Roots in Maize  

Kim, Chung Su (Department of Biological Sciences, Andong National University)
Mulkey, Timothy J. (Department of Biology, Indiana State University)
Kim, Soon Young (Department of Biological Sciences, Andong National University)
Publication Information
Journal of Life Science / v.24, no.12, 2014 , pp. 1364-1370 More about this Journal
Abstract
Ascorbic acid (AA) is a multifunctional metabolite in plants that is essential for plant development and growth. We examined the effect of AA, an antioxidant, on the gravitropic response of primary roots in maize. The application of $10^{-3}$ M AA to the elongation zone did not affect the gravitropic response and slightly inhibited the root growth. However, treatment with both $10^{-5}$ M and $10^{-3}$ M AA at the root tip increased the gravitropic response and inhibited root growth. Differences in indole-3- acetic acid (IAA) activity between the upper and lower hemispheres of the root resulted in differential elongation along the horizontal root. Roots are extremely sensitive to IAA, and increasing the amount of IAA in the lower hemisphere of the root inhibited elongation. Therefore, we examined the effect of IAA in the presence of AA. The inhibitory effect of AA on the gravitropic response was greater in combination with IAA. To understand the role of AA in the regulation of root growth and the gravitropic response, we measured ethylene production in the presence of AA in the primary roots of maize. AA stimulated ethylene production via the activation of the 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene, which regulates the conversion of ACC to ethylene. These results suggest that AA alters the gravitropic response of maize roots through modification of the action of ethylene.
Keywords
Ascorbic acid (AA); ACC oxidase; ethylene; gravitropic response;
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