• Title/Summary/Keyword: 생물리학적 측정

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Anti-inflammatory Effects of Aroma Oil Complex on DNCB-Induced Allergic Contact Dermatitis in Dogs (개에서 DNCB에 의해 유발된 알레르기성 접촉피부염에 대한 아로마 오일 합제의 항염증 효과)

  • Oh, Dong-Kyu;Oh, Tae-Ho
    • Journal of Veterinary Clinics
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    • v.31 no.3
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    • pp.180-193
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    • 2014
  • Allergic contact dermatitis (ACD) is an inflammatory skin disease and regarded as a prototype of T-cell mediated delayed-type hypersensitivity reactions. Aroma Oil Complex (AOC) is composed of lavender true oil, chamomile roman oil and tea tree oil. This study was performed to assess the effects of AOC in a canine model of ACD. ACD was induced on the back of dogs induced by sensitization and repeated application by 2,4-dinitro-1-chlorobenzene (DNCB). Topical treatment of AOC was applied once a day for 8 days and skin biophysical parameters including transepidermal water loss (TEWL), skin hydration, skin thickness and erythema index, were measured every two days during experimental periods. Histopathology and immunohistochemistry were performed to evaluate the anti-inflammatory effect. In skin biophysical parameters, TEWL, skin hydration, skin thickness and erythema index were significantly increased, with a maximum increase appeared on day 2 (p<0.05). After the completion of AOC treatment, skin biophysical parameters were significantly reached those of baseline in a time-dependent manner (p<0.05). In histopathology, marked increases of epidermal thicknesses were induced after DNCB challenge with numerous inflammatory cell infiltrations and edematous changes, decreases of connective tissue occupied regions in dermis. In addition, marked increases of cytokine - tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interferon-${\gamma}$ (IFN-${\gamma}$)-immunoreactivities in the dermis and of apoptotic markers - caspase-3 and PARP-immunoreactivities in the epidermis were observed in DNCB control as compared with intact control, respectively (p<0.01). The decrease of infiltrated inflammatory cells and related decreases of pro-inflammatory cytokine immunoreactivities were observed in AOC treated skin (p<0.01). Based on these findings, AOC may have anti-inflammatory and alleviatory effects in the allergic contact dermatitis.

Biostability Characterization in a Full-scale Nanofiltration Water Treatment System (대규모 나노여과 정수처리 시스템에서의 생물학적 안정성에 관한 연구)

  • Hong, Seung-Kwan;Escobar, Isabel C.;Cho, Jae-Weon
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.2
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    • pp.158-162
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    • 2005
  • The objective of this study was to assess the assimilable organic carbon (AOC) in processing water, a measurement of biostability, at several stages of a full-scale nanofiltration (NF) water treatment plant. The NF membrane plant investigated was a $45,400\;m^3$/day (12 mgd) water softening facility at Plantation City in southern Florida, which utilized an organic rich groundwater (dissolved organic carbon (DOC) = 17.6 mg/L) originated from a surficial aquifer. The average AOC concentration of raw feed water was estimated at 158 g/L acetate-C. After pretreatment(acid and antiscalant addition), AOC levels increased by 12.7%, suggesting that pretreatment chemicals used to control scaling may deteriorate feed water biostability. The results also demonstrated that nanofiltration was capable of effectively removing 63.4% of AOC and 94.8% of DOC from the raw water. AOC rejection in stage 1 (${\approx}\;68%$) was slightly higher than that of stage 2 (${\approx}\;58%$) indicating that AOC was removed less at the solution environment (i.e. low pH, high ionic strength and high hardness), which was often created in the $2^{nd}$ stage of full-scale membrane plants due to pretreatment (acid addition) and high recovery operation.

Effects of Advanced Oxidation of Penicillin on Biotoxicity, Biodegradability and Subsequent Biological Treatment (고도산화공정 처리가 페니실린의 생독성, 생분해도 및 생물학적 분해에 미치는 영향)

  • Luu, Huyen Trang;Minh, Dang Nhat;Lee, Kisay
    • Applied Chemistry for Engineering
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    • v.29 no.6
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    • pp.690-695
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    • 2018
  • Advanced oxidation processes (AOPs) composed of O3 and UV were applied to degrade penicillin (PEN). The degradation efficiency was evaluated in terms of changes in the absorbance (ABS) and total organic carbon (TOC). The combination of $O_3/H_2O_2/UV$ and $O_3/UV$ showed the best performance for the reduction of ABS (100% for 9 min) and TOC (70% for 60 min) values, although the mineralization was uncompleted under the experimental condition in this study. The change in biotoxicy was monitored with Escherichia coli susceptibility and Vibrio fischeri biofluorescence. The E. coli susceptibility was eliminated completely for 9 min by $O_3/UV$, and the toxicity to V. fischeri biofluorescence was 57% reduced by $O_3/H_2O_2/UV$. For the ultimate treatment of PEN, it is suggested that an AOP using $O_3/UV$ is followed by biological treatment, utilizing the enhanced biodegradability by the AOP. During 30 min of $O_3/UV$ treatment, the $BOD_5/COD$ ratio as an indication of biodegradability showed about 4-fold increment, compared to that of using a non-treated sample. TOC removal rate for AOP-pretreated PEN wastewater increased 55% compared to that of using the non-pretreated one through an aerobic biological treatment by Pseudomonas putida for artificial wastewater containing 20 mg/L of PEN. In conclusion, $O_3/UV$ process is recommended as a pretreatment step prior to an aerobic biological process to improve the ultimate degradation of penicillin.

Age-Specific Brain Activation in Secondary School Students' Self-Regulating Activities on Biological Tasks -fNIRS Study (생물 과제의 자기조절 활동에서 나타나는 중등학생의 연령별 두뇌 활성 -fNIRS 연구)

  • Lee, Seo-Ri;Kwon, Yong-Ju
    • Journal of Science Education
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    • v.46 no.1
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    • pp.30-39
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    • 2022
  • The purpose of this study is to compare and analyze secondary school student's brain activity on assimilation, conflict, and accommodation processes of self-regulation. The self-regulation task was presented a biological phylogenetic task, and the brain activity was measured and analyzed with fNIRS. As a result, a significant activation was found in the left DLPFC, OFC, and FP regions in the conflict process compared to the assimilation process, and a significant activation was found in DLPFC and VLPFC in the accommodation process. As the age increase, the DLPFC also increases in the conflict process and VLPFC increases in the assimilation process. In addition, comparing conflict and accommodation process, the 7th grade students show a significant brain activity in the right VLPFC, the 9th grade students show significant brain activity in the left FP and DLPFC areas in the accommodation process. However, the 11th grade students did not show any significant brain activity at this process. These results presumably show that the neurological research method could be applied to educational research in cognitive activity and classroom instructional situation.

Synthesis, Potentiometric, Spectral Characterization and Microbial Studies of Transition Metal Complexes with Tridentate Ligand (세자리 리간드의 전이금속 착물에 대한 합성과 전위차 및 분광학적 확인 그리고 미생물학적 연구)

  • Jadhav, S.M.;Munde, A.S.;Shankarwar, S.G.;Patharkar, V.R.;Shelke, V.A.;Chondhekar, T.K.
    • Journal of the Korean Chemical Society
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    • v.54 no.5
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    • pp.515-522
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    • 2010
  • A relation between antimicrobial activities and the formation constants of solid complexes of Cu(II), Ni(II), Co(II), Mn(II) and Fe(III) with tridentate Schiff base ligand, 4-hydroxy-3(1-{2-(benzylideneamino)-phenylimino}-ethyl)-6-methyl-2Hpyran-2-one (HL) derived from o-phenylene diamines, dehydroacetic acid (DHA) and p-chloro benzaldehyde have been studied. The ligand and metal complexes were characterized by elemental analysis, conductivity, magnetic susceptibility, thermal analysis, X-ray diffraction, IR, $^1H$-NMR, UV-vis and mass spectra. From the analytical data, the stiochiometry of the complexes was found to be 1:2 (metal:ligand) with octahedral geometry. The molar conductance values suggest the nonelectrolytic nature of metal complexes. The X-ray diffraction data suggests monoclinic crystal system for Ni(II) and orthorhombic crystal system for Cu(II) and Co(II) complexes. The IR spectral data suggest that the ligand behaves as tridentate ligand with ONN donor atoms sequence towards central metal ion. Thermal behavior (TG/DTA) and kinetic parameters calculated by Coats-Redfern method suggests more ordered activated state in complex formation. The protonation constants of the complexes were determined potentiometrically in THF:water (60:40) medium at $25^{\circ}C$ and ionic strength ${\mu}=0.1\;M$ ($NaClO_4$). Antibacterial activities in vitro were performed against Staphylococcus aureu and Escherichia coli. Antifungal activities were studied against Aspergillus Niger and Trichoderma. The effect of the metal ions and stabilities of complexes on antimicrobial activities are discussed.

Standardization of Hydration in the Stratum Corneum Using by Polyols (폴리올을 이용한 각질층 수분량 측정의 표준화 연구)

  • Nam, Gaewon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.2
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    • pp.113-119
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    • 2015
  • The measurement of hydration level in the surface layer of the skin, stratum corneum (SC), gives important information on the biophysical properties and function of the skin barrier such as softness, flexibility, and healthiness of the skin. But it is difficult to measure a consistent hydration level from a sample to another sample due to individual variations and environmental changes. The aim of this study was to evaluate objective hydration after using various products in the SC. The SC Hydration was measured by capacitance (Corneometer$^{(R)}$, C+K, Germany) on the ventral site of forearm from 40 healthy volunteers. The skin surface was chronologically measured immediately after application of the test products and 3 and 6 hours later. We analyzed the averages of five measurements of each site and used the hydration increase rate for correction on untreated site variation. We found that most polyols including glycerol and butylenes glycol influenced directly the hydration increase rate in the SC previously. In this study, glycerol was used to prepare the standard products from 0 to 20 percents and applied to the same volunteers. The individual standard curve showed linear relation to glycerol concentrations. Based on the the standard curve, hydration of SC was converted into hydration increase rate to glycerol concentrations. The converted glycerol concentrations of products were repetitive and reproducible. In addition, the individual standard curve was used to relate the skin type of each individual. These results suggest that the hydration of the SC standardized regardless of external variation and individual skin condition can explain detailed skin state variation. Further studies will be conducted with other ingredients such as surfactants, lipids and aqueous materials, and with other methods for noninvasive measurement.

Photochemical/Biophysical Properties of Proteorhodopsin and Anabaena Sensory Rhodopsin in Various Physical Environments (막 단백질인 Proteorhodopsin과 Anabaena Sensory Rhodopsin의 다양한 측정 환경에 따른 광화학/생물리학적 특성)

  • Choi, Ah-Reum;Han, Song-I;Chung, Young-Ho;Jung, Kwang-Hwan
    • Korean Journal of Microbiology
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    • v.47 no.1
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    • pp.22-29
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    • 2011
  • Rhodopsin is a membrane protein with seven transmembrane region which contains a retinal as its chromophore. Although there have been recently reports on various photo-biochemical features of rhodopsins by a wide range of purifying and measurement methods, there was no actual comparison related to the difference of biochemical characteristics according to their physical environment of rhodopsins. First, proteorhodopsin (PR) was found in marine proteobacteria whose function is known for pumping proton using light energy. Second one is Anabaena sensory rhodopsin (Nostoc sp.) PCC7120 (ASR) which belongs to eubacteria acts as sensory regulator since it is co-expressed with transducer 14 kDa in an operon. In this study, we applied two types of rhodopsins (PR and ASR) to various environmental conditions such as in Escherichia coli membranes, membrane in acrylamide gel, in DDM (n-dodecyl-${\beta}$-D-maltopyranoside), OG (octyl-${\beta}$-D-glucopyranoside), and reconstituted with DOPC (1,2-didecanoyl-sn-glycero-3-phosphocholine). According to the light-induced difference spectroscopy, rhodopsins in 0.02% DDM clearly showed photointermediates like M, and O states which respond to the different wavelengths, respectively and showed the best signal/noise ratio. The laser-induced difference spectra showed the fast formation and decay rate of photointermediates in the DDM solubilized samples than gel encapsulated rhodopsin. Each of rhodopsins seemed to be adapted to its surrounding environment.

Biological Applications of White Light Scanning Interferometry (백색광 주사간섭계의 생물학적 응용)

  • Kim, Ki-Woo
    • Applied Microscopy
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    • v.41 no.4
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    • pp.223-228
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    • 2011
  • White light scanning interferometry has been employed to analyze surface features of diverse specimens. Long established in the field of materials engineering, the technique provides quantitative three-dimensional data as well as qualitative morphological images. It uses white light that is split and reflected from a reference mirror and an object. Merged together, the light generates interference patterns representing topographical contours of the object surface. The amplitude of the z-axis data is differentiated by gray scale. The technique allows the rapid, noncontact, and wide-field measurements for morphometry of biological specimens including chondrocytes, tooth enamel, and plant leaves. Quantification of the dimension of surface structures such as width, length, and elevation angle could be achievable by white light scanning interferometry. The light reflection from plant leaves has been assumed to be sufficient for the technique. Without special specimen preparations like conductive metal coating, the technique can be increasingly used for quantitative three-dimensional surface measurements of biological specimens.

Evaluation of the preservation state of human skeletal remains using real-time PCR (출토 인골 DNA의 real-time PCR 정량에 의한 보존상태 평가 연구 - 부여 오수리 출토 인골을 중심으로 -)

  • Kwon, Eun-Sil;Cho, Eun-Min;Kim, Sue-Hoon;Kang, Soyeong
    • 보존과학연구
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    • s.32
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    • pp.171-183
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    • 2011
  • In this study molecular genetic analysis was carried out on 4 human skeletal remains from Osuri, Buyeo. We showed that real-time PCR is the method of the choice to assess the initial number of genuine ancient DNA molecules. Human mitochondrial DNA quantification was accomplished by the real-time PCR for the cytochrome b gene of the mitochondria. Histological results proved to be a good potentiality for biochemical analysis using biomolecule. The level of specimen's preservation state was proved that level of quantitative result was BO-04, BO-01, BO-03, BO-02. Continually, we showed that biochemical and biomolecule results for the level of preservation state were similar. This study will be useful to important material for predicting biochemistry and biology analysis of the ancient bone.

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