• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.69-74
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• 2008

This study aims to establish the efficient soybean transformation system and develop soybean [Glycine max (L.) Merill] transformants using cotyledonary node explants. The cotyledonary node of soybean were co-cultivated with Agrobacterium tumefaciens strains (KYRT1, EHA105). These strains contain the binary vector pCAMBIA3301 which carries a herbicide-resistant far gene. Korean cultivars (Danbaekkong, Eunhakong) and foreign cultivars (Jack, Peking) were the most efficient in regenerating cotyledonary node. Therefore, they were chosen for the transformation. Results showed that the T-DNA transfer reached up to 60% and transformation efficiency reached up to 3% in the cotyledonary node explants from Jack cultivar, co-cultivated with EHA105 strain. Histochemical GUS evaluation showed that 12 individual lines, transformed with the 현 gene, have positive response. The transformed soybeans have been confirmed in the $T_0$ generation through phenotypic assay using herbicide $Basta^{(R)}$ and Southern blot analysis.

• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.143-153
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• 2016

Plant regeneration via organogenesis and somatic embryogenesis was investigated in Korean soybean cultivars including Cheongja 3, Jinpumkong 2, Taekwangkong and Uram. Cotyledon, cotyledon+hypocotyl and hypocotyl segments of 7-day-old seedlings were cultured on MS medium containing various concentration (0, 1, 2 and 4 ㎎/L) of BA and TDZ. The results showed that MS medium supplemented with BA 2.0 ㎎/L yielded the highest shoot formation ratio of 83.3%. In 4 cultivars, Taekwangkong showed the highest ratio of shoot formation. When various sizes of immature cotyledons (S: 1∼ 2 ㎜, M: 3∼5 ㎜, L: 6∼8 ㎜) were tested on MS medium containing 2,4-D 40 ㎎/L for somatic embryogenesis, the optimum size for embryogenic callus induction was 3∼5 ㎜ in length of immature cotyledons. In 4 cultivars, Taekwangkong showed the highest percentage of embryogenic callus induction. The results indicate that Taekwangkong is the best soybean cultivar for plant regeneration via organogenesis and embryogenic callus induction among the 4 cultivars.

• Journal of Plant Biology
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• v.36 no.4
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• pp.399-406
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• 1993

유채(Brassica napus L. cv. Topas) 소포자 배양에 의한 기내 배발생 과정을 관찰하고 발생과정에 따른 총단백질의 변화 양상을 이차원 전기영동을 통하여 분석하였다. 배양에 적합한 소포자의 발달 단계는 4,6-diamidino-2-phenylindole (DAPI) 형광염색으로 핵상관찰을 통하여 결정하였다. 최초의 소포자 생존율은 63.9%였으며 이러한 생존율의 차이로 배양초기에 소포자의 이형성이 나타났다. 배 발달 경로에 따른 최초의 세포분열은 화분벽 안에서 균등분열로 시작되어 배병의 발달이 선행된 후 배 발생이 일어났으며, 구형, 심장형, 어뢰형으로의 배 발달은 여러 조직의 분화와 더불어 빠르게 진행되었다. 소포자배 발달과정을 치상단계의 소포자, 배양 3일째의 초기 분열세포, 구형 및 심장형배, 어뢰형배, 성숙한 자엽단계의 배 등의 5단계로 나누어 각각 2차원 전기영동을 수행한 결과, 최초 소포자 단계에서 나타났던 23개의 단백질들은 배 달달 경로로 진행됨에 따라 사라지고, 배양 3일째에는 8개의 단백질이 특이적 또는 지속적으로 발현되었다. 배의 발달과 더불어 20∼50 kD 사이에서 총 42개의 단백질이 급격히 나타나거나 또는 후기 배로 진행하면서 점차적으로 발현되었다.

• Applied Biological Chemistry
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• v.45 no.1
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• pp.47-52
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• 2002

In order to search for insulin-like substances from the constituted herbs of Sogal prescriptions, we selected 19 traditional herbs, based on a review of the Donguibogam. The effects of the hot-water extract from the selected herbs on the proliferation and the differentiation of 3T3-L1 fibroblasts were tested. The various water-extracts from Pinellia ternata, Magnolia obovata, Rheum palmatum, Acanthopanax sessiliflorun, Atractylodes japonica and Strychnos ignatii inhibited the proliferation of 3T3-L1 fibroblasts, did not influence entirely in differentiation of 3T3-L1 fibroblasts. Treatment of 3T3-L1 fibroblasts with the extract from Ephedra sinica, Trichosanthes kirilowii, Scrophularia buergeriana and Sophora flavescens significantly increased the differentiation of the cells. In conclusion, these may contain such compounds that play a role of insulin-like action.

• Korean Journal of Animal Reproduction
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• v.20 no.1
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• pp.43-52
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• 1996

This study was carried out to establish the in vitro short-term culture system of developing male germ cells by purifing germ cells of various stages. The decapulated testicular cells were incubated with collagenase (lmg/ml) and try psin (2.5mg/ml) in HBSS. After separating male germ cell, the separated germ cells were stained with heamatoxylin/eosin and determined developing stages under light microscopy. The purity of pachtene spermatocytes a and round spermatid were 85%, respectively. Yield of total male germ cells was highly variable between individuals, with a mean value of 3.5 to 4.5 ${\times}$ 10$^7$ cells/testis. Viability of the cell was over 97% after separation. In DMEM medium, the optimal cell number for culture is approximately 1 x 10$^5$ cells/dish, but low cell den-sities than 1 ${\times}$ 10$^5$ cell/dish showed a decreased cell viability. Furthermore, about :36.8% of pac-hytene cells was successfully cultured for 6 days and some of cells were developed to secondary spermatids and round spermatids. Therefore, our data suggested that this culture conditions will be utilize as a feasible tools to produce tran-sgenic livestock using techniques such as intrac-ytoplasmic injection and cell fusion.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.3
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• pp.209-212
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• 2002

We obtained regenerated Italian ryegrass (Lolium multiflorum Lam.) plants by anther culture. When Italian ryegrass anther was incubated for 20 days on callus induction medium, MS medium containing 30 g/$\ell$ of sucrose, 2 mg/$\ell$ of NAA and 1 mg/$\ell$ of kinetin, its callus was induced. The ratio of callus induction was 9.2 %, the mean of callus weight was 8.6 mg/callus/anther. When Italian ryegrass callus was incubated for 50 days on plant regeneration medium, MS medium containing 30 g/$\ell$ of sucrose, 1 mg/$\ell$of NAA and 2 mg/$\ell$of kinetin, Italian ryegrass plant was regenerated. The ratio of plant regeneration was 26%.

• Korean Journal of Food Science and Technology
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• v.42 no.5
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• pp.637-642
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• 2010

Bone is continuously remodeled by osteoblasts and osteoclasts. We investigated the effects of medicinal herbs, which act on bone metabolism. Fifteen kinds of medicinal herb extracts were screened for bone formation activity with osteoblastic cells, and MC3T3-E1 and bone resorption were screened with osteoclasts derived from mouse bone marrow macrophages. Among these samples, Actinidia polygama, Eucommia ulmoides Oliv., Schizonepeta tenuifolia, Sorbus commixta, and Zingiber officinale Rosc. extracts showed strong bone-forming activity accompanied with osteoblast proliferation and alkaline phosphatase activity. In addition, these extracts decreased tartrate-resistant acid phosphatase activity against osteoclast differentiation. The results indicate that these medicinal herb extracts can potentially prevent bone-related diseases such as osteoporosis by increasing osteoblast differentiation and reducing osteoclast activity.

• Journal of Dental Rehabilitation and Applied Science
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• v.25 no.2
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• pp.191-200
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• 2009

Dental Pulp Stem Cells are superior to other types of adult stem cell. Because of teeth are easy to access and are extracted throughout life. A supernumerary tooth is an important clinical problem found in various populations of the world. The incidence of supernumerary teeth varies depending on the literature source. Pediatric dentists are routinely extracted them. However, no studies have been reported regarding Dental Pulp stem cells in a supernumerary tooth, and we failed to note that a valuable source of human stem cell. Herein, we tried to show that a supernumerary tooth contains cells that display the characteristic features of stem cells.

• Korean Journal of Plant Tissue Culture
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• v.28 no.2
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• pp.97-101
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• 2001

The transport and allocation of photosynthetic assimilate is an important regulatory factor in plant productivity, In order to modify assimilate partitioning in rice, transgenic plants containing a potato sucrose transporter (SuT) gene were developed. Calli derived from rice seeds (Oryza sativa L. cv Dongjin) were cocultured with A. tumefaciens LBA 4404 harboring the SuT gene. Calli were transferred to MS medium supplemented with 50 mg/L hygromycin, 500 mg/L carbenicillin, 2 mg/L kinetin, 0.1 mg/L NAA. After 2 weeks, hygromycin resistant shoots were obtained from the calli on the selection medium. Roots were induced from the putative transgenic shoots on rooting MS medium supplemented with 250 mg/L cabenicillin. Plant regeneration rate from the calli was about 150%. Stable incorporation of the potato SuT gene into rice genomic DNA was confirmed by PCR and Southern blot analysis.

• Journal of Plant Biotechnology
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• v.29 no.1
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• pp.25-29
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• 2002

The study was carried out to establish an efficient protocol for shoot organogenesis and plant regeneration from stem explant cultures of Lycopersicon esculentum cv. Micro-Tom. The regenerated shoots obtained from stem explant cultures on solid MS medium containing the different concentrations of BAP. The highest number of shoots (5.3) per explant and shoot growth (0.7 cm) was obtained on MS medium containing 4.0 mg/L BA. The additions of AgNO$_3$ and putrescine substantially improved the shoot regeneration frequency, at the optimal concentration of 7 mg/L and 50 mg/L respectively. The regenerated shoots (about 1 cm) were normal and could be easily rooted with 0.1 mg/L IBA treatment. The rooted plants were hardened and transferred to vermiculite with a 92% survival rate where they grew normally.