Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Production of New Regenerated Plant by Anther Culture of Italian Ryegrass

약(葯)배양 기법 이용한 이탈리안 라이그라스 식물체 생산

  • Published : 2002.09.01
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Abstract

We obtained regenerated Italian ryegrass (Lolium multiflorum Lam.) plants by anther culture. When Italian ryegrass anther was incubated for 20 days on callus induction medium, MS medium containing 30 g/$\ell$ of sucrose, 2 mg/$\ell$ of NAA and 1 mg/$\ell$ of kinetin, its callus was induced. The ratio of callus induction was 9.2 %, the mean of callus weight was 8.6 mg/callus/anther. When Italian ryegrass callus was incubated for 50 days on plant regeneration medium, MS medium containing 30 g/$\ell$ of sucrose, 1 mg/$\ell$of NAA and 2 mg/$\ell$of kinetin, Italian ryegrass plant was regenerated. The ratio of plant regeneration was 26%.

이탈리안 라이그라스(Lolium multiflorum Lam.) 계통 A의 약(葯) 배양을 실시하여 약(葯) 유래의 식물체를 생산하였다. MS 기본배지에 sucrose 30 g/$\ell$, NAA 2 30 mg/$\ell$, kinetin 1 30 mg/$\ell$을 첨가한 캘러스 유도배지에서 배양 20일만에 약(葯) 유래의 캘러스를 유도하였다. 캘러스 유도율은 9.2%였으며, 캘러스의 평균 크기는 8.6mg/callus/anther 였다. MS 기본배지에 sucrose 30 g/$\ell$, NAA 1 mg/$\ell$, kinetin 2 mg/$\ell$을 첨가한 재분화 배지에 캘러스를 치상하여 배양 50일에 완전히 재분화된 식물체를 얻었으며, 캘러스로부터 식물체로 재분화되는 비율은 26% 였다.

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References

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