Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Transformation of Rice (Oryza sativa L.) with Sucrose Transporter cDNA from Potato (Solanum tuberosum L.)

감자 Sucrose Transporter 유전자의 벼 Genome 내로의 도입

  • 백소현 (전북대학교 농업과학기술연구소) ;
  • 유남희 (전북대학교 농업과학기술연구소, (주)그린바이오텍 생명공학연구소) ;
  • 윤성중 (전북대학교 농업과학기술연구소, 전북대학교 농과대학 생물자원과학부)
  • Published : 2001.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

The transport and allocation of photosynthetic assimilate is an important regulatory factor in plant productivity, In order to modify assimilate partitioning in rice, transgenic plants containing a potato sucrose transporter (SuT) gene were developed. Calli derived from rice seeds (Oryza sativa L. cv Dongjin) were cocultured with A. tumefaciens LBA 4404 harboring the SuT gene. Calli were transferred to MS medium supplemented with 50 mg/L hygromycin, 500 mg/L carbenicillin, 2 mg/L kinetin, 0.1 mg/L NAA. After 2 weeks, hygromycin resistant shoots were obtained from the calli on the selection medium. Roots were induced from the putative transgenic shoots on rooting MS medium supplemented with 250 mg/L cabenicillin. Plant regeneration rate from the calli was about 150%. Stable incorporation of the potato SuT gene into rice genomic DNA was confirmed by PCR and Southern blot analysis.

동화산물의 분배 효율 개선을 통한 생산성 향상 가능성을 조사하고자 감자의 sucrose 수송자 유전자를 벼에 형질전환 하였다. 동진벼로부터 유도된 callus를 감자의 sucrose 수송자 유전자가 도입된 A. tumefaciens LBA 4404와 공동배양한 후, 선발배지에서 증식된 callus를 250 mg/L carbenicillin, 2 mg/L kinetin, 0.1 mg/L NAA가 첨가된 MS 배지에 옮겨 약2주 후부터 소식물체를 얻었다. Carbenicillin 250 mg/L 첨가된 MS 기본 배지에서 소식물체의 발근을 유도하여 재분화 식물체를 얻었다. 선발된 callus는 약 150%의 높은 식물체 재분화율을 보였다. 재분화 식물체에 대한 PCR 분석을 수행하여 감자의 sucrose수송자 유전자가 삽입된 형질전환 벼 식물체를 선발하였다. 선발된 형질전환 식물체에 대한 Southern blot 분석을 통하여 외래유전자인 감질 sucrose 수송자 유전자가 벼 genome 내에 안정적으로 도입되었음을 확인하였다.

Keywords

References

  1. Plant Molecular Biology Mannual v.A3 Binary vectors An G;Ebert PR;Mitra A;Ha SB
  2. J. Theor. Biol. v.21 no.1 The selection of sucrose as the translocate of higher plants Arnold WN
  3. Plant Cell Physiol. v.40 no.10 Molecular cloning and expression analysis of a gene for a sucrose transporter in maize (Zea mays L.) Aoki N;Hirose T;Takahashi S;Ono K;Ishimaru K;Ohsugi R
  4. Plant Physiol. v.78 Membrane transport in isolated vesicles from sugarbeet taproot Briskin DP;Thornley WR;Wyse RE
  5. Proc. Natl. Acad. Sci. USA v.95 Sucrose is a signal molecule in assimilate partitioning Chiou TJ;Bush DR
  6. Scienta Sinic. v.18 Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources Chu CC;Wang CC;Sun CS;Hsu C;Yin KC;Chu CY;Bi FY
  7. Plant J. v.9 no.1 Expression of the PmSUC1 sucrose carrier gene from Plantago major L. is induced during seed development Gahrtz M;Schmelzer E;Stolz J;Sauer N
  8. Protoplasma v.200 Spatial and temporal expression of sucrose transport-related genes in developing cotyledons of Vica faba L. Harrington GN;Nussbaumer Y;Wang XD;Tegeder M;Franceschi VR;Frommer WB;Patrick JW;Offler CE
  9. Plant Cell Physiol. v.38 no.12 cDNA cloning and tissue specific expression of a gene for sucrose transporter from rice (Oryza sativa L.) Hirose T;Imaizumi N;Scofield GN;Furbank RT;Ohsugi R
  10. Nucleic Acids Research v.21 no.17 A simple method of preparing plant samples for PCR Hong W;Meiqing Q;Adrian JC
  11. Biochimica et Biophysica Acta v.1465 Sucrose transporters in plants: update on function and structure Lemoine R
  12. FEBS Lett. v.454 no.3 Identification of a pollen-specific sucrose transporter-like protein NtSUT3 from tobacco Lemoine R;Burkle L;Barker L;Sakr S;Kuhn C; Regnacq M;Gaillard C;Delrot S;Frommer WB
  13. Plant Physiol. v.100 Plasma membrane vesicles from souce and sink leaves-changes in solute transport and polypeptide composition Lemoine R;Gallet O;Gaillard C;Frommer W;Delrot S
  14. Plant Physiol. v.124 no.1 Sugar uptake and transport in rice embryo. Expression Of companion cell-specific sucrose transporter (OsSUT1) induced by sugar and light Matsukura Ca;Saitoh T;Hirose T;Ohsugi R;Perata P;Yamaguchi J
  15. Plant J. v.24 no.6 AtSUC3, a gene encoding a new Arabidopsis sucrose transporter, is expressed in cells adjacent to the vascular tissue and in a carpel cell layer Meyer S;Melzer M;Truemit E;Hummer C;Besenbeck R;Stadler R;Sauer N
  16. Physiol. Plant v.15 A revised medium for rapid growth and bioassays with tobacco tissue cultures Murashige T;Skoog F
  17. Plant Physiol. v.122 no.4 The sucrose transporter of celery. Identification and expression during salt stress Noiraud N;Delrot S;Lemoine R
  18. EMBO J. v.11 no.13 Isolation and characterization of a sucrose carrier cDNA from spinach by functional expression in yeast Riesmeier JW;Willmitzer L;Frommer WB
  19. Plant Cell. v.5 Potato sucrose transporter expression in minor veins indicates a role in phloem loading Riesmeier JW;Hirner B;Frommer WB
  20. Iniplant Molecular Biology Manual Extraction of DNA from plant tissues Rogers SO;AJ Bendich;Geliben(et al.)(ed.)
  21. Plant Physiol. v.103 Effect of cutting on solute uptake by plasma membrane vesicles from sugar beet (Beta vulagris L) leaves Sakr S;Lemoine R;Gaillard C;Delrot S
  22. Plant J. v.6 no.1 SUC1 and SUC2: two sucrose transporters from Arabidopsis thaliana; expression and characterization in baker's yeast and identification of the histidine-tagged protein Sauer N;Stolz J
  23. J. Mol. Biol. v.98 Detection of specific sequences among DNA fragments separated by gel electrophoresis Southern EM
  24. Plant J. v.19 no.3 The AtSUC1 sucrose carrier may represent the osmotic driving force for anther dehiscence and pollen tube growth in Arabidopsis Stadler R;Truernit E;Gahrtz M;Sauer N
  25. Int Rev Cytol. v.178 Sucrose transport in higher plants Ward JM;Kuhn C;Tegeder M;Frommer WB
  26. Korean J. Plant Tissue Culture v.27 no.6 Transformation of rice (Oryza sativa L.) with phosphate transporter cDNA from tobacco(Nicotiana tabacum L.) Yoo NH;Yun SJ