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http://dx.doi.org/10.7732/kjpr.2016.29.1.143

Plant Regeneration by in vitro Tissue Culture in Korean Soybean (Glycine max L.)  

Kim, Dong-Gun (Graduate School, Sunchon National University)
Kantayos, Vipada (Graduate School, Sunchon National University)
Kim, Dong-Kwan (Institute of Agricultural Science and Technology, Jellaname-do Agricultural Research and Extension Services)
Park, Heung-Gyu (Institute of Agricultural Science and Technology, Jellaname-do Agricultural Research and Extension Services)
Kim, Haeng-Hoon (Department of Well-being Bioresources, Sunchon National University)
Rha, Eui-Shik (Department of Well-being Bioresources, Sunchon National University)
Lee, Sheong Chun (Department of Well-being Bioresources, Sunchon National University)
Bae, Chang-Hyu (Department of Well-being Bioresources, Sunchon National University)
Publication Information
Korean Journal of Plant Resources / v.29, no.1, 2016 , pp. 143-153 More about this Journal
Abstract
Plant regeneration via organogenesis and somatic embryogenesis was investigated in Korean soybean cultivars including Cheongja 3, Jinpumkong 2, Taekwangkong and Uram. Cotyledon, cotyledon+hypocotyl and hypocotyl segments of 7-day-old seedlings were cultured on MS medium containing various concentration (0, 1, 2 and 4 ㎎/L) of BA and TDZ. The results showed that MS medium supplemented with BA 2.0 ㎎/L yielded the highest shoot formation ratio of 83.3%. In 4 cultivars, Taekwangkong showed the highest ratio of shoot formation. When various sizes of immature cotyledons (S: 1∼ 2 ㎜, M: 3∼5 ㎜, L: 6∼8 ㎜) were tested on MS medium containing 2,4-D 40 ㎎/L for somatic embryogenesis, the optimum size for embryogenic callus induction was 3∼5 ㎜ in length of immature cotyledons. In 4 cultivars, Taekwangkong showed the highest percentage of embryogenic callus induction. The results indicate that Taekwangkong is the best soybean cultivar for plant regeneration via organogenesis and embryogenic callus induction among the 4 cultivars.
Keywords
Callus; Organogenesis; Somatic embryogenesis; Soybean; TDZ;
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