• The Journal of Korean Obstetrics and Gynecology
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• v.24 no.1
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• pp.74-86
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• 2011

Objectives: The purpose of this study was to examine correlation between Heart Rate Variability and urine analysis of women with urinary disturbance. Methods: We studied 34 patients visiting ${\bigcirc}{\bigcirc}$hospital from January 2010 to September 2010. The subjects were categorized in two groups, symptom group (n=11) and no symptom group(n=23). We studied the difference of Heart Rate Variability and urine analysis between two groups by Student T-test and correlation between Heart Rate Variability and urine analysis by Pearson's correlation coefficient test using SPSS for windows (version 17.0). Results: Occult blood of symptom group was significantly higher than no symptom group. SDNN, TP and HF of symptom group was significantly lower than no symptom group. Occult blood and SDNN, occult blood and RMS-SD, occult blood and HF significantly showed negative correlation coefficient. pH and TP, pH and LF significantly showed positive correlation coefficient. Conclusion: The results suggest that urinary disturbance can be related to decreased activity of autonomic nervous system. Also urine from women with urinary disturbance tend to show higher occult blood.

• The Journal of Korean Obstetrics and Gynecology
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• v.25 no.2
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• pp.120-130
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• 2012

Objectives: The purpose of this study is to investigate the symptoms accompanied with acne and HRV pattern of the acne patients. Methods: We reviewed 60 acne patients(age 15-38) who visited ${\bigcirc}{\bigcirc}$ hospital from January 2010 to October 2011. We investigated the symptoms accompanied with acne of 60 patients and evaluated HRV of 41 acne patients. The data were analyzed by Mann-Whitney test using SPSS/PC ver 13.0 program. Results: 1. Prevalence of female post-adolescent acne was 66.7% in this study. The symptoms accompanied with acne were dischezia(35%), gastric discomfort(28.3%) in all patients and dysmenorrhea(40%), leukorrhea(38%), irregular menstrual period (24%) in female patients. 2. SDNN, RMSSD, VLF and HF of female were significantly lower than male. 3. SDNN, TP and VLF of un-dysmenorrhea group were significantly lower than dysmenorrhea group. 4. PSI of under 6 months group was significantly higer than over 6 months group. 5. In postadolescent acne patients SDNN, RMSSD, TP, LF and HF were significantly lower and PSI was significantly higher than adolescent acne patients. Conclusions: There are many female post-adolescent acne pateints and the assessment of the symptoms accompanied with acne and stress degree is helpful in treating acne. The further research and development about the diagnostic and therapeutic methods of acne are needed in oriental obstetrics and gynecology field.

• The Journal of Korean Obstetrics and Gynecology
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• v.24 no.1
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• pp.42-62
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• 2011

Objective: This study was performed to investigate the effect of acupuncture at HT8 on brain activity in perimenopausal women using fMRI. Methods: 15 healthy perimenopausal women volunteered in the study. No stimulation, sham stimulation, duration of acupuncture treatment on HT8, and rotation of acupuncture treatment on HT8 were randomly given for 6 minutes, with 20 seconds' intervals. Results: 1. In comparison with sham stimulation(Sham-B) and duration of acupucture (S1-B, S1-Sham, S2-S1), the areas of fMRI signal activation areas were just like cases including no stimulation. But the areas of vision were activated in S1-Sham. 2. In comparison with duration of acupuncture(S1-B, S1-Sham, S2-S1) and rotation of acupuncture(S2-B, S2-Sham, S2-S1), the areas of vision were activated in duration of acupuncture, and Supplementary motor area(SMA) were especially activated in rotation of acupuncture. Conclusions: After using fMRI and analysing effect of acupuncture treatment at HT8, we could confirm that fMRI signal activation areas by acupuncture treatment at HT8 were different from areas by sham stimulation. And according to acupuncture stimulation methods with duration and rotation, etc, we could confirm the specific reactions of series, and could get useful basic data for research of acupuncture from now on.

• The Journal of Korean Obstetrics and Gynecology
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• v.24 no.4
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• pp.174-185
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• 2011

Objectives: The purpose of this study is to investigate all postpartum care center in Seoul about its state of doctors' linkage and oriental medical treatment. Methods: From May 1st 2011 to July 10th 2011, survey was done using a selfmade questionnaire by phone or visit. There were 12 categories including the state of doctors' linkage in postpartum care centers, treatment form of oriental medical doctors, utilization of herbal medicine and so on. The data collected was analyzed using Excel 2007 FOR WINDOWS. Results: 1. The doctors' linkage with postpartum centers included pediatricians(96.1%), oriental medicine doctors(61.2%), and gynecologists(34.0%). Of those postpartum centers, 66% had both pediatrician and oriental medical doctor which were the vast majority. 2. Out of 70 postpartum centers providing herbal medicine, 9 of them were supplied of herbal medicine by herb sellers(not doctor). Where as, at 63 oriental medical doctors-affiliated centers, 85.7% of them had oriental medical doctors' examinations. 3. Out of 70 postpartum centers providing herbal medicine, personalized prescription amounted to 35(50.0%), same prescriptions amounted to 34(48.6%), and 1(1.4%) refused to answer. 4. Out of all the patients that received explanation about the effects of herbal medicine, 62.9% had them explained by oriental medical doctors. Conclusions: These findings suggests that oriental medical doctors are actively involved with postpartum care centers but problems surfaced regarding individualized prescription insufficiency and the lack of administration. In the near future, if the expertise expand in the areas of pediatrics as well as obstetrics and gynecology, oriental medicine will have a more solid role in postpartum care part.

• The Journal of Korean Obstetrics and Gynecology
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• v.25 no.2
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• pp.89-107
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• 2012

Objectives: This study was performed to develop therapeutic prescription that is more significant than existing ones through extraction method and formulation changes. Methods: Yukmijihwangtang(YMJHT) was extracted in 80% ethanol, and their relative anti-oxidant activities as well as anti-inflammatory effects through immune modulation were measured. Results: Both water and ethanol extracted YMJHT showed does-dependent DPPH elimination activities. ROS inhibition activity was greater in water extracted YMJHT except for Moutan Cortex. NO inhibition assay results indicated that all groups showed higher NO inhibition activities in RAW 264.7 cells in dose dependent manner. Water extracted group showed higher NO inhibition activity than that of ethanol extracted group. TNF-${\alpha}$ secretion inhibition assay using RAW 264.7 cells, water extracted YMJHT showed higher activity than ethanol extracts. Growth rate of spleen cells was greater in all tested groups, with higher rate in YMJHT-EtOH than YMJHT-DW. Suppression of gene expression of IFN-r in spleen cells stimulated by Con A was higher in YMJHT-EtOH than YMJHT-DW. Suppression of gene expression of IL-10 in spleen cells stimulated by Con A was highest in YMJHT-DW with 40%. Suppression of gene expression of IL-4 in spleen cells stimulated by Con A were significant with 90% or higher in all groups and that of IL-12p35 were also higher than 90% in all cases. Conclusions: From the results, it shows that YMJHT has anti-inflammatory effects through immune modulation. However, the difference between YMJHT-EtOH and YMJHT-DW was not that significant. Further studies are needed to find out effective extraction methods of herbal medicine.

• The Journal of Korean Obstetrics and Gynecology
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• v.24 no.1
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• pp.27-41
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• 2011

Objectives: This study was designed to investigate the effect of Ojeok-san(OJS) on the estradiol valerate(EV)-induced polycystic ovaries(PCO) in rats. Methods: PCO was induced by single intramuscular injection with estradiol valerate(EV)(4mg) in female rats. Normal group(n=8) were injected with sesame oil and orally administrated distilled water for sixty days. PCO control group (n=8) were injected with EV and orally administrated distilled water for sixty days. OJS treated group(n=8) were injected with EV and orally administrated OJS for sixty days. Then, we measured weights of body, ovaries, adrenal glands and uterus of rats. The histomorphometrical changes of ovaries were also evaluated. And we observed the NGF and CRF expression by immunohistochemistry. Results: The results were as follows - The weights(mg) of ovaries in OJS treated group($57.4{\pm}9.4$) were significantly increased(p < 0.05) compared with PCO control group($42.3{\pm}8.5$). - The numbers of mature follicles in OJS treated group($9.3{\pm}2.5$) were significantly increased(p < 0.05) compared with PCO control group($6.1{\pm}2.1$). - The expressions of NGF-immunoreactive cells in the ovarian granulosa cells in OJS treated group were weaker than PCO control group. Conclusion: From the these results, we concluded that Ojeok-san(OJS) has inhibitory effect on the development of EV-induced polycystic ovary. And it's effect maybe related with decreased NGF activities in the ovary.

• The Journal of Korean Obstetrics and Gynecology
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• v.24 no.1
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• pp.15-26
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• 2011

Objectives: The purpose of this study was to investigate the anti-inflammatory effects of aqueous extract from Cicadidae Periostracum(CP) on the RAW 264.7 cells. Method: We examined the cytokine productions including nitric oxide(NO), interleukin(IL)-1b, IL-6 and tumor necrosis factor-a(TNF-a) in lipopolysaccharide (LPS)-induced RAW 264.7 cells and also inhibitory mechanisms such as mitogen -activated protein kinases(MAPKs) and nuclear factor kappa BNF-kB) using Western blot. Results: CP inhibited LPS-induced production of NO, IL-1b and TNF-a but not of IL-6 in RAW 264.7 cells. CP respectively inhibited the activation of MAPKs such as extracelluar signal-regulated kinase(ERK 1/2), c-Jun $NH_2$-terminal kinase(JNK), p38 and NF-kB in the LPS-stimulated RAW 264.7 cells. Also oral administration of CP inhibited CLP - induced endotoxin shock. Conclusion: our results showed that CP down-regulated LPS-induced NO, IL-1b and TNF-a productions mainly through ERK, JNK, p38 MAPK and NF-kB pathway, which suggest the anti-inflammatory effects of CP.

• The Journal of Korean Obstetrics and Gynecology
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• v.25 no.2
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• pp.66-77
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• 2012

Objectives: This study was designed to investigate the effects of PR on ovarian tissue in PCOS rats through measurement of morphological and histo-pathological observations, ovarian size. In addition, effects on expression levels of Insulin like Growth Factor Receptor(IGFR) were also investigated to elucidate related mechanisms. Methods: PCOS was induced by single intermuscular injection with ${\beta}$-Estradiol 17-Valerate(EV) in female rats. Normal group(NOR, n=8) were injected with sesame oil and administrated hard food for five weeks. Control group(CTL, n=8) were injected with EV and administrated hard food for five weeks. CR group(n=8) were injected with EV and administrated hard food mixed CR for five weeks. Then, we measured weights of body and ovary, uptakes of food and water. And we observed morphological and histo-pathological changes of ovary, levels of IGFR. Results: In this experiments, single injection of Estradiol Valerate(EV) induced suppression of weight gain, formation of cysts, increase of IGFR expression. Oral administration of PR prevent decrease of ovarian size significantly. Further more, formation of cystic follicles induced by EV injection is suppressed by PR treatment. Conclusions: These results suggest PR can be used for patients with PCOS to prevent formation of cystic follicles and malfunction of ovary.

• The Journal of Korean Obstetrics and Gynecology
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• v.24 no.4
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• pp.150-162
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• 2011

Purpose: To investigate the change of the climacteric syndrome with women with induced menopause by hysterectomy or ovariectomy compared with women with natural menopause. Methods: Menopausal women(aged 45-55) were selected at two oriental medicine hospitals, Sam-se Oriental Medicine Hospital and Daegu Hannny Oriental Medicine Hospital. They were asked about Kupperman's Index, MENQOL and a few additional questions. They were divided into two groups: women with induced menopause by hysterectomy or ovariectomy and women with natural menopause. To analyse the Kupperman's index and MENQOL, SPSS for Windows 12.0 and Wilcoxon Ranked Sum Test were used. Results: The total sums of Kupperman's index and MENQOL were significantly correlated with women with induced menopause and women with natural menopause and women with induced menopause had higher total sums. Both the renal and urinary tract disorder and the sexual disorder were significantly correlated with women with induced menopause and women with natural menopause. The gastrointestinal and psychosocial disorder of Kupperman's index and MENQOL were not significantly correlated with women with induced menopause and women with natural menopause. Conclusions: Women with induced menopause by hysterectomy or ovariectomy experienced climacteric syndrome more severely compared with women with natural menopause, specifically the renal and urinary tract disorder and sexual disorder.

• The Journal of Korean Obstetrics and Gynecology
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• v.25 no.2
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• pp.12-22
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• 2012

Objectives: The aim of this study is to investigate immune enhancing effect of Houttuyniae Herba water extract(HW) on RAW 264.7 cell of mouse macrophages. Methods: Effects of HW on productions of nitric oxide(NO) and hydrogen peroxide($H_2O_2$) in RAW 264.7 mouse macrophages were measured. Effect of HW on production of cytokines such as interleukin(IL)-$1{\beta}$, IL-6, and tumor necrosis factor(TNF)-${\alpha}$ in RAW 264.7 cells was accessed by a multiplex bead array assay based on xMAP technology. All of results were represented P<0.05 compared to the normal. Results: 1. After 24 hr incubation, HW increased significantly NO production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ${\mu}g$/mL. 2. After 24 hr incubation, HW increased significantly hydrogen peroxide production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ${\mu}g$/mL. 3. After 24 hr incubation, HW increased significantly IL-$1{\beta}$ production in RAW 264.7 cells at the concentrations of 100 and 200 ${\mu}g$/mL. 4. After 24 hr incubation, HW increased significantly IL-6 production in RAW 264.7 cells at the concentrations of 100 and 200 ${\mu}g$/mL. 5. After 24 hr incubation, HW increased significantly TNF-${\alpha}$ production in RAW 264.7 cells at the concentrations of 50, 100, and 200 ${\mu}g$/mL. Conclusions: These results suggest that HW has immune enhancing activity related with its increasement of NO, hydrogen peroxide, IL-$1{\beta}$, IL-6, and TNF-${\alpha}$ in macrophages.