The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Immune Enhancing Effect of Houttuyniae Herba on Mouse Macrophage

어성초(魚腥草)의 면역활성에 미치는 영향

  • Kim, Jeong-Hyun (Dept. of Gynecology, College of Oriental Medicine, Ga-Chon University) ;
  • Kim, Yoon-Sang (Dept. of Gynecology, College of Oriental Medicine, Ga-Chon University) ;
  • Lim, Eun-Mee (Dept. of Gynecology, College of Oriental Medicine, Ga-Chon University)
  • 김정현 (가천대학교 한의과대학 한방부인과교실) ;
  • 김윤상 (가천대학교 한의과대학 한방부인과교실) ;
  • 임은미 (가천대학교 한의과대학 한방부인과교실)
  • Received : 2012.04.25
  • Accepted : 2012.05.15
  • Published : 2012.05.25
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Abstract

Objectives: The aim of this study is to investigate immune enhancing effect of Houttuyniae Herba water extract(HW) on RAW 264.7 cell of mouse macrophages. Methods: Effects of HW on productions of nitric oxide(NO) and hydrogen peroxide($H_2O_2$) in RAW 264.7 mouse macrophages were measured. Effect of HW on production of cytokines such as interleukin(IL)-$1{\beta}$, IL-6, and tumor necrosis factor(TNF)-${\alpha}$ in RAW 264.7 cells was accessed by a multiplex bead array assay based on xMAP technology. All of results were represented P<0.05 compared to the normal. Results: 1. After 24 hr incubation, HW increased significantly NO production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ${\mu}g$/mL. 2. After 24 hr incubation, HW increased significantly hydrogen peroxide production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ${\mu}g$/mL. 3. After 24 hr incubation, HW increased significantly IL-$1{\beta}$ production in RAW 264.7 cells at the concentrations of 100 and 200 ${\mu}g$/mL. 4. After 24 hr incubation, HW increased significantly IL-6 production in RAW 264.7 cells at the concentrations of 100 and 200 ${\mu}g$/mL. 5. After 24 hr incubation, HW increased significantly TNF-${\alpha}$ production in RAW 264.7 cells at the concentrations of 50, 100, and 200 ${\mu}g$/mL. Conclusions: These results suggest that HW has immune enhancing activity related with its increasement of NO, hydrogen peroxide, IL-$1{\beta}$, IL-6, and TNF-${\alpha}$ in macrophages.

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References

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