Browse > Article
http://dx.doi.org/10.15204/jkobgy.2012.25.2.089

Evaluation of Anti-Inflammatory Effects of Yukmijihwangtang and Individual Drug Substances Based on the Extraction Methods  

Lee, Gui-Hee (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University)
Yoo, Dong-Youl (Dept. of Oriental Gynecology, College of Oriental Medicine, Dae-Jeon University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.25, no.2, 2012 , pp. 89-107 More about this Journal
Abstract
Objectives: This study was performed to develop therapeutic prescription that is more significant than existing ones through extraction method and formulation changes. Methods: Yukmijihwangtang(YMJHT) was extracted in 80% ethanol, and their relative anti-oxidant activities as well as anti-inflammatory effects through immune modulation were measured. Results: Both water and ethanol extracted YMJHT showed does-dependent DPPH elimination activities. ROS inhibition activity was greater in water extracted YMJHT except for Moutan Cortex. NO inhibition assay results indicated that all groups showed higher NO inhibition activities in RAW 264.7 cells in dose dependent manner. Water extracted group showed higher NO inhibition activity than that of ethanol extracted group. TNF-${\alpha}$ secretion inhibition assay using RAW 264.7 cells, water extracted YMJHT showed higher activity than ethanol extracts. Growth rate of spleen cells was greater in all tested groups, with higher rate in YMJHT-EtOH than YMJHT-DW. Suppression of gene expression of IFN-r in spleen cells stimulated by Con A was higher in YMJHT-EtOH than YMJHT-DW. Suppression of gene expression of IL-10 in spleen cells stimulated by Con A was highest in YMJHT-DW with 40%. Suppression of gene expression of IL-4 in spleen cells stimulated by Con A were significant with 90% or higher in all groups and that of IL-12p35 were also higher than 90% in all cases. Conclusions: From the results, it shows that YMJHT has anti-inflammatory effects through immune modulation. However, the difference between YMJHT-EtOH and YMJHT-DW was not that significant. Further studies are needed to find out effective extraction methods of herbal medicine.
Keywords
Yukmijihwangtang(YMJHT); anti-oxidant activities; anti-inflammatory effects; immune modulation; extraction methods;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Tabibzadeh S. Human endometrium an active site of cytocines production and action. 1991;12:272.   DOI
2 Brian T et al. The effect of fetal acidemia on fetal-placental vascular tone and production of inflammatory cytokines interleukin-6 and tumor necrosis $factor-{\alpha}$. American Journal of Obstetrics and Gynecology. 2002; 187(4):894-7.   DOI
3 Charles N et al. Expression of cyclooxygenase-2 in cervical, endometrial, and ovarian malignancies. American Journal of Obstetrics and Gynecology. 2003;188(5):1174-6.   DOI
4 지병철, 문신용. Nitric Oxide가 인간황체화 과립막세포의 아포프토시스에 미치는 영향. 대한산부회지. 2002;45(5):766-73.
5 Ryu, JH et al. Inhibitory activity of plant extracts on nitric oxide synthesis in LPS- activated macrophage. Phytother Res. 2003;17:485-9.   DOI
6 Mu MM et al. The inhibitory action of quercetin on lipopolysaccharideinduced nitric oxide production in RAW 264.7 macrophage cells, J. Endotoxin Res. 2001;7:431-8.   DOI
7 이준경 등. EBM 구축을 위한 육미지황탕 문헌 분석 연구. 동의생리병리학회지. 2009;23(1):15-26.
8 정은경 등. 육미지황탕 전탕액투여가 백서의 현장기능에 미치는 영향. 한의생리학회지. 1996;11:73-9.
9 정은경 등. 육미지황탕 전탕액투여가 백서의 혈장Renin활성도. 혈장Aldosterone 및 Atrial Natriuretic Peptide 농도에 미치는 영향. 대한한의학회지. 1997;18:449-55.
10 최우정 등. 左歸飮과 육미지황탕 전탕액투여가 家免신기능 및 혈장 Aldosterone 농도에 미치는 영향. 동의생리병리학회지. 2002;16:1263-9.
11 Rho SW et al. Effects of Yukmijihwnang -tang derivatives(YMJD), a memory enhancing herbal extract, on the gene-expression profile in the rat hippocampus. Biol Pharm Bull. 2005; 28:87-93.   DOI
12 Wei XL. Studies on learning and memory function-related genesin the hipocampus and the relationship between the cognitive enhancing effect of liuweidihuang decoction(LW) and gene expression. Sheng Li Ke Xue Jin Zhan. 2002;31 :227-30.
13 陳紀藩. 金匱要略. 北京:人民衛生出版社. 2000:203.
14 錢乙. 小兒藥證直結. 서울:의성당. 2002:32.
15 Yang S et al. Effects of LiuweiDihuang decoction on ion channels and synaptic transmission in cultured hippocampal neuron of rat. Journal of Ethnophamacology. 2006;106:166-72.   DOI
16 김민정, 홍권의. 加味歸茸湯 및 육미지황탕 약침이 생쥐의 성장에 미치는 영향. 대한약침학회지. 2007;9(3):105-16.
17 이상곤, 김완희. 四物湯과 육미지황탕이 난소적출로 유도된 백서의 골다공증에 미치는 영향. 동의생리학회지. 1997;12(1):1-18.
18 김우현 등. 육미지황탕 投與가 Rat의 成長 및 血淸總Choleterol含量에 미치는 影響, 경산한의대논문집. 1978;1:111-5.
19 서은경 등. 비만 실험동물 쥐에서의 육미지황탕의 항 당뇨효과. Korea Journal of Life Science. 2000;10:388-96.
20 Wu YC et al. Increase of insulin sensitivity in diabetic rats received Die-Huang-Wan, a herbalmixture used in Chinese traditional medicine. Acta Pharmacol Sin. 2022;23:1181-7.
21 한일수, 김철중. 八味地黃湯 및 加味地黃湯이 생쥐의 복강 대식세포활성에 미치는 영향. 대전대한의과대학원논문집. 1997;6:331-47.
22 전진오, 정현우. 육미지황탕이 면역세포에 미치는 실험적 효과. 대한한방내과학회지. 2005;21:243-50.
23 박성민 등. 補中益氣湯과 육미지황탕이 노화촉진생쥐(SAM)의 간장 내 항산화 작용에 미치는 영향. 대한본초학회지. 2003;18:175-91.
24 서영은 등. 육미지황탕구성약물이 PC12세포의 산화억제에 미치는 영향. 동의생리병리학회지. 2003;17:203-8.
25 Zhao L et al. Inhibitory effect of liuweidihuang decoction on induced mutation and spontaneous tumor. Zhong Xi Yi Jie He Za Zhi. 1990; 10:433-5.
26 김길훤, 김완희. 운동부하후의 피로회복에 미치는 補中益氣湯 및 六味地黃湯의 효과. 동의생리학회지. 1987;1-13.
27 한용주 등. 다용 한약처방 투여가 흰쥐의 간 기능에 미치는 영향. 대한한의학회지. 2006;27:78-90.
28 Oh MS et al. Yukmijihwangtang protects against cyclophosphamide-induced reproductive toxicity. Reproductive Toxicology. 2007;24:365-70.   DOI
29 Jiang TL et al. Preventing effect of "Liuweidihuangdecoction" on esophageal carcinoma. Gan To Kagaku Ryoho. 1989;16:1511-8.
30 김우식, 김동희. 육미지황탕煎湯掖이 家免血壓 및 白鼠肝TBA에 미치는 영향에 대한 연구. 대전대한의과대학논문집. 1979;2:142-52.
31 이정원. 간질성방광염에 대한 육미지황탕가미방의 실험적 연구. 동국대학교 대학원 박사학위논문. 2010.
32 대한병리학회. 병리학II. 서울:고문사. 1995:71-104, 112-20, 540-2.
33 표명윤, 정애희. Mancozeb이 마우스비장세포의 IFN-r 생성능에 미치는 영향. 환경독성보건학회, 2005;20(3):243-8.
34 Rollins BJ and JS Pober. Interleukin-4 induces the synthesis and secretion of MCP-1/JE by human endothelial cells. Am. J. Pathol, 1991;138:1315-9.
35 Rollins BJ et al. Cytokine-activated human endothelial cells synthesize and secrete a monocyte chemoattractant, MCP-1/JE. Am. J. Pathol. 1990;136 :1229-33.
36 임준희, 권택규. Luteolin의 $IL-1{\beta}$에 의한 MCP1 단백질 발현 증가에 미치는 영향. 생명과학회지. 2009;19(4):514-9.
37 김상찬, 변성희. 백화사설초가 Ovalbumin으로 유도된 천식동물 모델에서 Eosinophil의 수, IgE 및 IL-4에 미치는 영향. 대한본초학회지. 2005;20(2):35-42.
38 강재성 등. 세포분자면역학 제5판. 서울:범문사. 2004:255, 261, 337, 494.
39 瀋鴻鵠. 중의학치료염증학. 군사의학과학기술출판사. 2000:3-15, 329, 650,683.
40 한의부인과학 편찬위원회. 한의부인과학(상). 서울:정담출판사. 2002:67,164, 271-80.
41 한방약리학 교재편찬위원회. 제 3판 한방 약리학. 서울:신일북스. 2010:9.
42 김경임 등. 천마 분말, 에탄올 및 열수추출물이 고지방식이를 급여한 흰쥐의 혈청지질과 체지방에 미치는 영향. 한국식품과학회지. 2003;35(4):720-5.
43 채우석. 면역질환의 한방개념과 치료에 관한 문헌적 고찰. 대한한의학회지. 1990;11(2):54-8.
44 이상민 등 : 추출방식에 따른 防風通聖散이 지방세포 대사에 미치는 영향. 대한약침학회지. 2008;11(1):163-75.
45 홍원식. 精校黃帝內經素問. 서울:동양의학연구원출판사. 1985:38, 55, 78, 86-8, 104, 107, 110, 116-24, 213, 233, 247, 256, 273.
46 홍원식. 精校黃帝內經靈樞, 서울:동양의학연구원출판사. 1981:317, 347.
47 황의옥 등. 면역학에 관한 문헌적 고찰. 대한한의학회지. 1989;10(1):193-226.
48 조종관. 면역에 관한 동양의학적 고찰. 동양의학. 1986;12(1):19-23.
49 한의과대학 방제학교실. 방제학. 서울:영림사. 2003:298.
50 김시영, 이인선. 한방부인과영역에서 六味地黃湯과 附益地黃丸의 효능에 관한 문헌적 고찰. 대한한방부인과학회지. 1997;10(1):133-50.
51 조문경. 골반염치료의 최신지견. 대한산부회지. 2010;53(11):961-6.
52 McCord JM. Free radicals and inflammation, protection of synorial fluid by superoxide dismutase. Science. 1974;185:529-31.   DOI
53 Harman D. Freeradicaltheory of aging:Role of freeradicals in the organization and evolution of life, aging and disease process. New York:Free Radicals, Aging and Degenerative Disease. Inc. 1986 :3-49.
54 Cheng ZJ et al.. Antioxidantat properties of butein isolated from Dalbergia odorifera. Biochim Biophys Acta. 1987;1392:291-9.
55 Aeberi D et al. Inhibition of the TNFpathway use of infliximab and etanercept as remission-inducing agents in case of therapy-resistant c hrsonic inflammatory disorders. Swiss Med.Wkly. 2002;132 :414-22.
56 Feldman M et al. $Anti-TNF-{\alpha}$ therapy is useful in rheumatoid art hrsitis and Crohn's disease: analysis of the mechanism of action predicts utility in other disease, Transplant Proc. 1998;30:4126-7.   DOI