• Title/Summary/Keyword: 본엽

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Micropropagation by Apical Meristem Culture of Wasabia japonica Matsum (고추냉이의 頂端分裂組織培養에 의한 微細增殖)

  • 은종선;고정애;김영선;김명준
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.1
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    • pp.43-48
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    • 1997
  • Apical meristems of Wasabia japonica were cultured on Murashige and Skoog's medium supplemented with cytokinins alone or together with 1.0 mg/L IAA. Shoot initials could be induced from leaf primordia on apical meristems. Calli and roots were formed on the medium containing cytokinins and 1.0 mg/L IAA in combination after 30 days of culture, but there were no callus proliferation. Shoot organogenesis began after 60 days of culture and these small shoots elongated when transferred to a medium containing 1.0 mg/L BA or kinetin. Shoots were formed directly without callus induction from apical meristems all the explants on the medium containing cytokinins variously, and most of the shoots proliferated multiple shoots which could be divided to obtain plantlets. Shoot multiplication rate in response to cytokinins was best on the medium containing 1.0 mg/L BA or 2.0 mg/L zeatin. Divided plantlets rooted well on MS medium containing 0.01 mg/L IBA after 15~30 days of subculture and the rooted plantlets developed into whole plants with multiple shoots. After rooting, the regenerated plants were washed and transferred to the pots containing sterilized soil.

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Gamma Radiation Sensitivity and Quantitative Characters in M1 Generation of Heteropappus hispidus (Thunb.) (갯쑥부쟁이(Heteropappus hispidus (Thunb.))의 감마방사선 감수성과 M1 세대 형질변동 특징)

  • Oh, Byung-Kwon;Hong, Kyung-Ae;Song, Sung-Jun;Lee, Sun-Joo;Lee, Young-Il;U., Zang-Kual
    • Korean Journal of Environmental Agriculture
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    • v.22 no.4
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    • pp.294-299
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    • 2003
  • The seeds of naturally growing Heteropappus hispidus (Thunb.) were treated by nine different doses (0, 10, 20, 40, 80, 120, 160, 200, 300, 400 Gy) of gamma rays to investigate their germination rate and to quantity the characteristics of their germinated plants as like as leaf appearance and length, the formation rate of anthocyanin color in stem 30 days after germination, the formation rate of rosette leaf and multi-shoot, the flowering and seed-bearing, and shoot length. The germination rate at least up to 120Gy was not greatly affected but was rapidly decreased at over 160Gy. It seemed that lethal dose ($LD_{50}$) of germination was 160Gy. The leaf appearance and growth was also inhibited, but the formation rate of anthocyanin color in the flower stem was enhanced up to 30% with dose. The rosette plants were observed in plants irradiated with higher than 40Gy. Multi-shoots were developed over 80Gy. For a short shoot length and bundle of thin stem, it was considered that they can be selected as the potential pot flower plants, through genetic fixation. In particular, it was suggested that the formation of anthocyanin color in flower stem, rosette and multi-shoot plants induced by the high dose of gamma rays could be utilized as the morphological markers for the mutant selection of Heteropappus hispidus (Thunb.).

Studies on the Varietal Differences of Tolerance to Cold Damage in Seedling Stage of the Rice Plant (수도 유묘기 내냉성의 품종간 차이에 관한 연구)

  • Chung-Ik Cho;Seong Kuk Bae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.21 no.1
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    • pp.35-42
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    • 1976
  • In Korea, two types of rice varieties are being cultivated widely, Japonica and Japonica-Indica cross. Generally speaking, the latter shows weak cold tolerance than the former and so brought about many problems in cultural practices. This study was conducted to investigate the effect . of various temperature conditions on rice seedlings growth, especially the contents of chlorophyll and the percentage of nitrogen. And the result obtained indicated the varieties of Japonica-Indica intend to weak cold tolerance than Japonica varieties.

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Herbicidal Activity of Naturally Developed d-Limonene against Sicyos angulatus L. under the Greenhouse and Open Field Condition (온실 및 노지조건에서 천연물질 d-Limonene의 가시박에 대한 살초활성)

  • Kang, Chung-Kil;Oh, Young-Joo;Lee, Sang-Beom;Lee, Byung-Mo;Nam, Hong-Shik;Lee, Yong-Ki;Jee, Hyeong-Jin;Hong, Moo-Ki;Koo, Suk-Jin
    • Korean Journal of Weed Science
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    • v.31 no.4
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    • pp.368-374
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    • 2011
  • This experiment was conducted to Investigate the herbicidal activity of naturally developed d-Limonene on the control of Sicyos angulatus L. which is destructive to biodiversity and ecosystem under greenhouse and open field condition. Among the tested concentrations, most efficacy of early foliar application(leaf stage with 1.5 and 3) of d-Limonene was found in $70mg\;mL^{-1}$, followed by $35mg\;mL^{-1}$, $17.5mg\;mL^{-1}$ in that order under greenhouse condition. The higher the d-Limonene concentration, the better the effect in greenhouse condition. In leaf stage with 2, 3 and 5 under open field condition, the higher the d-Limonene concentrations, the higher the weeding effect. The remarkable effect was, particularly, found in d-Limonene with $70mg\;mL^{-1}$ while little was found in the plants with d-Limonene with $35mg\;mL^{-1}$. In the case of 15 leaf stage of S. angulatus L. treated with d-Limonene under outside treatment effect was low. The treatment effect of d-Limonene under open field compared with greenhouse condition was more significant. It can be recommended that d-Limonene with $70mg\;mL^{-1}$ could control S. angulatus L. by 5 leaf stage.

Effect of Shading Using Different Colored Fabrics on Burcucumber(Sicyos angulatus L.) Seedling Growth and Cotyledon Chlorophyll Content (차광막의 색상이 안동대목 유묘의 생장과 자엽의 엽록소 함량에 미치는 영향)

  • Cho, Gun-Hyun;Jeon, Seung-Ho;Lee, Chang-Woo;Kim, Hong-Young;Kang, Jin-Ho
    • Korean Journal of Plant Resources
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    • v.20 no.5
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    • pp.466-470
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    • 2007
  • Nowadays burcucumber(Sicyos angulatus L.) has been widely used as a rootstock of Cucurbitaceae. The study was done to examine the effects of shading with different colored fabrics(white, red and aluminum-coated) on growth and cotyledon chlorophyll content of its seedling. Shading with the white colored fabric lengthened seedling height and hypocotyl length, but shading with the red colored fabric enlarged true leaf area. Total dry weight of each seedling was decreased in the order of no shading, white, red and aluminum, in which resulted from reductions of cotyledon dry weight in white colored fabric, cotyledon and true leaf dry weight in red colored fabric, and dry weight of cotyledon, true leaf and root in aluminum colored fabric. Cotyledon chlorophyll content was reduced by shading treatments although the 3 shading treatments showed nearly same content.

Effects of Growth Retardants B995 and CCC on the Growth arid Yield of Soybean (생장 조절제(B995.CCC) 산포가 대두의 생육 및 수량에 미치는 영향)

  • 위성옥;최원열
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.3
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    • pp.285-290
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    • 1984
  • The effects of retardants B995 and CCC on the growth and yield of soybean cultivar, kwanggyo, were investigated. The 3 application times(4th, 5th and 6th trifoliate leaf stage) combined with 4 concentrations (500, 1000, 2000 and 3000 ppm) were treated, and the retardant solutions were sprayed on the foliage. Only stem length in retardants, rate of podding in application times, and, in concentrations, stem length, number of branch, number of pod per plant and grain yield, were significant. In the inhibition of stem elongation, B995 was more effective than CCC, and rate of podding was the highest in the 4th leaf-spraying. The highest concentration, 3000 ppm, was the most effective in the inhibition of stem elongation and in the increase of number of branch and pod, rate of podding, and in yield. The number of node of mainstem, dry stem weight, chlorophyll and 100 grain weight were not significant with any treat. Yield had positively correlated with number of branch and pod, and rate of podding but negatively with stem length. The retardants was thought to be considerably conducive to the increase of yield.

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Effect of Transplanting Methods on Growth and Yield of Paprika in Coir Culture (코이어 배지 수경재배에서 정식방법이 파프리카 생육과 수량에 미치는 영향)

  • Kim, Cho Hee;Lee, Change Hee;Kweon, Oh Yeol;An, Chul Geon
    • Journal of Bio-Environment Control
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    • v.23 no.4
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    • pp.281-287
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    • 2014
  • This study was carried out to investigate the effect of transplanting methods on the growth and yield of paprika (Capsicumannuum L. 'Veyron' and 'Coletti') in coir culture during two seasons. The summer type sowed in late winter and harvested from summer and the winter type sowed in summer and harvested from early winter. Control plants grown on the 10cm rockwool block were transplanted on coir slab when the 8 leaves of seedlings were emerged, while plants of the young seedling transplanting(YST) grown on the 7cm rockwool block was put on the slab at time of 2-3 leaves developed. Plants of the temporary transplanting(TT) on the 10cm rockwool block were moved on the slab after 2-3 weeks underpinning cultivation, while plants of the blockless transplanting(BT) were directly transplanted in the slab when the seedlings have 2-3 leaves emerged. The plant height of the control and BT treatment were longer while that of TT showed the shortest among treatments. The bigger leaf size was observed in the YST and BT treatment. Leaf number of the BT treatment was increased, while that of TT was the lowest. There were no differences in fruit size, locules and thickness among treatments. The lower fruit weight was observed in the TT of the winter culture and fruits in the control and YST of the summer culture showed higher fruit weight. The percentage of marketable fruit appeared to be slightly higher in the winter culture than in the summer culture. There were no differences in marketable fruit rate among the treatments of the winter culture but, among the summer culture, the highest marketable fruit rate was observed in the BT with 93%. The yield of the YST and BT was higher and that of the TT was the lowest.

Growth and Critical Light Intensity at Cotyledon Stage of Cornus controversa Hemsl. Seedling (층층나무 자엽단계(子葉段階) 유묘(幼苗)의 생장(生長)과 한계광도(限界光度)에 관(關)한 연구(硏究))

  • Cho, Jae Hyoung;Hong, Sung Gak;Kim, Jong Jin
    • Journal of Korean Society of Forest Science
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    • v.87 no.3
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    • pp.493-500
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    • 1998
  • To investigate the effects of light intensity on the growth, and the critical minimum light intensity for growing of Cornus controversa seedlings at the stage of cotyledon, hypocotyl elongation, cotyledon expansion, the times of leaves appearance, dry weights of each organ, and specific leaf area(SLA) were measured on a growth chamber with several light intensity gradients(385, 32, 17, 8, and $5{\mu}mol\;m^{-2}s^{-1}$). There was a positive correlationship between the size of cotyledon and the biomass of cotyledon and total seedling. Hypocotyl was more elongated under relatively low light intensities, such as 32, 17, 8, and $5{\mu}mol\;m^{-2}s^{-1}$ than under $385{\mu}mol\;m^{-2}s^{-1}$ light intensity, however, dry weight of the hypocotyl was adverse. As the light intensities decreased, the leaf appearance was delayed and the number of leaves decresed. In addition, leaves did not appear under $8{\mu}mol\;m^{-2}s^{-1}$ and $5{\mu}mol\;m^{-2}s^{-1}$ light intensity. Although cotyledons were more fully expanded under 32 and $17{\mu}mol\;m^{-2}s^{-1}$ light intensities than $385{\mu}mol\;m^{-2}s^{-1}$ light intensity, the dry weights of cotyledons were greater under the high light intensity. The dry weight of cotyledon, hypocotyl, root and leaves showed a decreased pattern with decreasing light intensities, but root to shoot(hypocotyl+leaves) ratio rapidly increased. Roots did not develop below $8{\mu}mol\;m^{-2}s^{-1}$ light intensity. In conclusion, the results showed that the critical minimum light intensity for growing of Cornus controversa seedlings was above $17{\mu}mol\;m^{-2}s^{-1}$ light intensity.

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Fundamental Access to Sap Analysis for the Establishment of Nutrient Diagnosis Method (영양진단법 확립을 위한 즙액분석법의 기초적 검토)

  • 장홍기;정순주
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 1997.05a
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    • pp.11-16
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    • 1997
  • 본 연구는 양액재배에서 영양진단에 기초한 배양액관리를 하기 위해 유효한 수단으로 이용되고 있는 신속하고 간편하며 정확한 즙액분석 방법(질산태질소의 정량방법, 인산정량방법, 즙액추출법, 추출비율, 마쇄시간 및 샘플채취 부위 등)들에 관한 비교검토를 하였다. 'Earl's Favorite 춘계 F$_1$호'를 1993년 1월 11일에 파종하여 본엽 3~4매의 묘를 스티로폼베드내 암면슬라브에 정식하여 비순환방식으로 재배했다. 즙액분석법은 결핍이나 과잉증상이 외관적으로 나타나기 전에 신속하고 간이한 즙액분석법에 의해 작물체의 영양상태를 진단하여 대응이 가능하다고 판단되었다. 즙액분석시 질산태질소의 분석에는 자외부흡광도법, 인산분석에는 바나드몰리브덴산법이 추천되었고, 추출비율은 1:4로, 엽병의 마쇄시간은 60초, 그리고 채취 부위는 일정부위로 하는 것이 바람직하다고 판단되었다. 그러나 샘플채취시간에 대해서는 채취시간에 따른 차이가 크기 때문에 앞으로 보다 상세한 검토가 요청되었다.

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