• Journal of Digital Convergence
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• v.10 no.7
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• pp.267-272
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• 2012

Overcoming harsh light environment, as well as increased growth of crops even in high-quality production can play an important role when using the LED light system of photosynthetic products will be able to effectively reduce consumption. In this study, low efficiency of farm greenhouses, growing annual reduction in income due to rising operating costs and increase crop growth by inducing the proper planting environment Factory-type raise farmers' income and at the same time will contribute to the increase of Light device using LED Supplemental through photosynthesis, promote and improve product quality, plant growth regulators are considered possible for them to develop more efficient LED devices and LED Optical processing devices of Light leaf lettuce grown using normal fluorescent or incandescent bulbs grown in the results than the growth can see that the speed improvements. Usually shipped from seedling to harvest leaf lettuce from 25 to 30, whereas optical processing device be required red light (wavelength: 645nm) using a leaf of lettuce grown enough to be harvested after seven days increased the rate of growth. In addition, red light (wavelength: 645nm) and blue light (wavelength: 470nm) emitting at the same time, room, and grown for 5 days to harvest the growth rate was fast enough.

• Horticultural Science & Technology
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• v.34 no.1
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• pp.84-93
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• 2016

Plant factories with artificial lights require a large amount of electrical energy for lighting; therefore, enhancement of light use efficiency will decrease the cost of plant production. The objective of this study was to enhance the light use efficiency by using filters to diffuse the light from LED sources in plant factory conditions. The two treatments used diffuse glasses with haze factors of 40% and 80%, and a control without the filter. For each treatment, canopy light distribution was evaluated by a 3-D ray tracing method and canopy photosynthesis was measured with a sealed acrylic chamber. Sixteen lettuces for each treatment were cultivated hydroponically in a plant factory for 28 days after transplanting and their growth was compared. Simulation results showed that the light absorption was concentrated on the upper part of the lettuce canopy in treatments and control. The control showed particularly poor canopy light distribution with hotspots of light intensity; thus the light use efficiency decreased compared to the treatments. Total light absorption was the highest in the control; however, the amount of effective light absorption was higher in treatments than the control, and was highest in treatment using filters with a haze factor of 80%. Canopy photosynthesis and plant growth were significantly higher in all the treatments. In conclusion, application of the diffuse glass filters enhanced the canopy light distribution, photosynthesis, and growth of the plants under LED lighting, resulting in enhanced the light use efficiency in plant factory conditions.

• Journal of Applied Biological Chemistry
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• v.64 no.3
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• pp.197-203
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• 2021

Saponarin is a crucial component of barley sprout, and the production and quantitative analysis are issued to date. In this study, the optimal saponarin extraction conditions were presented on the subject of acetonitrile, ethanol, methanol, and water for the quantitative analysis in barley sprout through the extraction efficiency compared with the solvent concentration and extraction time using the reaction surface methodology. The optimal extraction time and solvent condition for saponarin were 3.9 h and 53.7% of aqueous methanol, respectively. In addition, the effect of LED artificial light on the saponarin production in barley sprouts was evaluated by the light cycle, light quantity, and light quality. The optimal cultivation conditions under artificial light for the growth of barley sprout and saponarin production were most effectively achieved on 220-320 μmol m^-2 s^-1 of the light quantity with 8 h day^-1 of a daylight cycle under 6500K LED combined with red light. Furthermore, blue light was evaluated as the main factor in the biosynthesis of saponarin.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.139-145
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• 2006

The objectives of this study was to determine the oil contents and fatty acid compositions of soybean germplasms including major Korean cultivars, and to provide the basic information of fatty acid composition for development high quality soybean varieties. Among 46 Korean major cultivated soybean samples, the oil contents were varied from 15.8% to 20.3%, and the average oil content was 18.2%. Crude oil content of cv. Saebyolkong (20.3%) was the highest among that of other cultivated soybeans. Likely many other oils of plant origin, most fatty acids in soybean are unsaturated. The highest percentage of fatty acid in soybean was linoleic acid (18:2), followed in a decreasing order by oleic (C18:1), palmitic (C16:0), linolenic (C18:3), and stearic acid (C18:0). The composition of C18:1 and C18:2 fatty acids among 563 soybean germplasms and cultivars which were positively correlated with nutritional quality of edible vegetable oil, were ranged $13.29{\sim}43.30%\;and\;38.21{\sim}61.51%$, respectively, and the C18:3 which were negatively correlated with flavor quality were varied from 5.03% to 11.48%. Also, the composition of C16:0 and C18:0 which were known to raise total cholesterol levels in human serum were ranged $8.50{\sim}14.78%\;and\;1.86{\sim}4.78%$ respectively. On the other hand, the range of fatty acid composition among 46 Korean major soybean cultivars has been investigated to be: C16:0, $8.96{\sim}13.23%$; C18:0, $2.55{\sim}4.20%$; C18:1, $16.33{\sim}36.41%$; C18:2, $42.32{\sim}58.84%$; C18:3, $6.98{\sim}10.72%$. Among the 563 germplasms tested, there are significant correlations among unsaturated fatty acids: negative between C18:1 and C18:2 (r=-0.961**) or C18:3 (r=-0.756**), and positive between C18:2 and C18:3 (r=0.608**). Also, the C18:1 was negatively correlated with saturated fatty acids, C16:0 (r=-0.231**) and C18:0 (r=-0.479**).

• Journal of Bio-Environment Control
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• v.22 no.1
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• pp.49-54
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• 2013

In this study, I was focusing on LED (Light Emitting Diode) light effect in growth of chrysanthemum. For this reason, I formed six monochromatic lights (red 650 nm, 647 nm, 622 nm, blue 463 nm, 450 nm, white), six mixed lights sources red : blue (9 : 1, 8 : 2, 7 : 3, 6 : 4, 5 : 5) and 3 control beds in light sources ratio between rad : blue (8 : 2) including sun light. It was totally 15 control beds. Depending on light investigation time in growth, 6/6 (on/off) was highest in the length of plant, the number of leaves, the fresh dry and leaf area. But statistical significance wasn't accepted in general. In case of monochromatic lights, length of plant and leaf area is biggest in the Blue 450 mm and the length of root is highest in RED 650 mm. Except for this 3 measuring points (length of plant, the number of leaves and fresh weight), sun light and white was highest. Besides there are monochromatic light effect but various wavelength range in light sources are needed to crop growth. In terms of mixed light resources, except for sun light, It turned out the length of plant is highest in the highest red light rate red : blue (9 : 1), and Red : white (7 : 3) is highest in fresh weight and dry weight. The sun light is the highest one in the leaf area. The results from LED light effect in growth of chrysanthemum are obviously effect on growth and building up the shape. We need to choose suitable light sources in the monochromatic lights and mixed lights for growing high quality of chrysanthemum or Supplemental Lighting.

• Horticultural Science & Technology
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• v.32 no.4
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• pp.448-453
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• 2014

Influences of shading and irrigation in summer hot pepper cultivation on the plant growth and mesophyll tissue were investigated. Hot pepper plants were exposed to three shade levels (0, $30{\pm}5$ and $80{\pm}5%$) and irrigated or non-irrigated in greenhouse condition. Plant height and leaf area were highest in 30% shading and stem diameter and fresh and dry weights were highest in no shading. Plant growth was better in rain shelters with irrigation than in those without irrigation. The numbers of hot pepper fruits in the beginning of harvest were 49 in rain shelters without irrigation and shading, 22 in those with irrigation and without shading, 5 in those without irrigation with 30% shading, and 1 in those with irrigation and 30% shading. However, 80% shading showed lowest flower number and flower abscission, resulting in no fruit set, regardless of irritation. This is because carbohydrate translocation from leaves to reproductive organs may be not enough for developing fruits due to the lack of sunlight. The yield of hot pepper tended to be higher in rain shelter with irrigation than in those without irrigation. In optical microscopy observation, the thickness and development of mesophyll tissues decreased as increasing the degree of shading but no effect of irrigation on mesophyll tissues was observed. When stomata were observed with scanning electron microscope (SEM), the shape of stomata was normal but tissues surrounding stomata were slightly wrinkled in plants grown under 30% shading. The large number of abnormal stomata and wrinkled leaves was observed among plants grown in rain shelters with 80% shading. In plants grown in rain shelters without irrigation, tissues surrounding stomata were wrinkled and 10-20% decrease in the number of stomata was observed. Therefore, in hot pepper cultivation in summer with high temperature, shading was not effective for fruit yield and mesophyll tissue development; if shading is unavoidable, high degree of shading is not advisable. Further studies are needed for appropriate cultivar selection and environment-control techniques in hot pepper cultivation in summer with high temperature.

• The Korean Journal of Nuclear Medicine
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• v.36 no.3
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• pp.195-202
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• 2002

Purpose: For clinical application of beta-emitter labeled antibody, high specific activity is imporiant. Carrier-free $^{188}Re$ from $^{188}W/^{188}Re$ generator is an ideal radionuclide for this purpose. However, low stability of $^{188}Re$ labeled antibody, especially in high specific activity, due to radiolytic decomposition by high energy (2.1 MeV) beta ray was problem. We studied the stability of $^{188}Re$ labeled antibody, and stabilizing effect of several stabilizers. Materials and Methods: Pre-reduced monoclonal antibody (CEA79.4) was labeled with $^{188}Re$ by incubating with generator-eluted $^{188}Re-perrhenate$ in the presence of stannous tartrate for 2 hr at room temperature. Radiochemical purity of each preparation was determined by chromatography. Human serum albumin was added to the labeled antibodies (2%). Stability of $^{188}Re-CEA79.4$ was investigated in the presence of ascorbic acid, ethanol, of Tween 80 as stabilizing agents. Results: Labeling efficiencies were $88{\pm}4%\;(n=12)$. Specific activities of $1.25{\sim}4.77MBq/{\mu}g$ were obtained. If stored after purging with $N_2$, all the preparations were stable for 10 hr. However, stability decreased in the presence of air. Perrhenate and $^{188}Re-tartrate$ was major impurity in declined preparation. colloid-formation was not a significant problem in all cases. Addition of ascorbic acid stabilized the labeled antibodies either under $N_2$ or under air by reducing the formation of perrhenate. Conclusion: High specific activity $^{188}Re$ labeled antibody is unstable, especially, in the presence of oxygen. Addition of ascorbic acid increased the stability.

• Journal of Bio-Environment Control
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• v.29 no.2
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• pp.171-180
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• 2020

The effect of three different light qualities on growth, photosynthesis, quality and safe parameters of hydroponic cultivated spinach (Spinacia oleracea L.) were investigated indoor. Three different light qualities were created of red (660 nm), blue (450 nm) and green (550 nm) LEDs corresponding at ratio R660/B450 = 4/1 (RBL); R660/B450/G550= 5/2/3 (WWL); R660/B450/G550 = 1/1/1 (WL), which were tested at the same intensity (PPFD =190 μmol m^-2 s^-1). The results showed that the plant height and leaf number were the lowest in WL treatment. The SPAD, Net photosynthesis rate Pn, Fv/Fm, Leaf area index LAI values and all parameters of root characteristics were the highest in RBL treatment and were significantly different from two others. Fresh weight of stem, leaf and root, dry weight of root in the three light qualities were significantly different. In contrast, the highest K⁺ content in WL was different from WWL and RBL treatments, while Ca²⁺ and Fe²⁺ content were the highest in the RBL treatment. Vitamin C content was significantly different between the three treatments. nitrate and oxalic acid contents were the highest in WL treatment, whereas soluble-solids contents and vitamin C contents were the highest in RBL treatment. Oxalic acid, nitrate contents were observed tending reduced under WWL although oxalic acid content in RBL treatment was not different from WL and WWL treatments. In all three different light treatments were not detected Salmonella, E.coli. Our results suggest that RBL may be appropriate light for growth of spinach, but supplementary green light to a combination of red and blue LEDs at the reasonable rate can change the quality of spinach in a positive direction. Hydroponic cultivated spinach was safe for users.

• The Korean Journal of Nuclear Medicine
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• v.34 no.4
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• pp.294-302
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• 2000

Purpose: To investigate the mechanisms related to F-18-FDG uptake by tumors, F-18-FDG accumulation was compared with glucose transporter-1 (Glut-1) expression and hexokinase activity in various human cancer cell lines. Materials and Methods: Human colon cancer (SNU-C2A, SNU-C4, SNU-C5), hepatocellular carcinoma (SNU-387, SNU-423, SNU-449), lung cancer (NCI-H522, NCI-H358, NCI-H1299), uterine cervical cancer (HeLa, HeLa 229, HeLa S3) and brain tumor (A172, Hs 683) cell lines were used. After 24 hr incubation of $5{\times}10^5$ cells, 37 kBq F-18-FDG was added and the uptake by cells at 10 min was measured using a gamma counter. Hexokinase activity was measured by continuous spectrophotometric rate determination. To measure mitochondrial hexokinase activity, mitochondrial fraction was separated by a high speed centrifuge. Immunohistochemical staining of Glut-1 was performed, and graded as 0, 1, 2, or 3 according to expression. Results: There was difference among F-18-FDG uptake, total and mitochondrial hexokinase activity, and Glut-1 expression with different cancer cell lines. The correlations of F-18-FDG with total hexokinase and mitochondrial hexokinase activity were low (r=0.27 and 0.26, respectively). Glut-1 expression showed a good correlation with F-18-FDG uptake (p=0.81, p=0.0015). Previously, we reported no correlation of F-18-FDG uptake with hexokinase activity in colon cancer cell lines. Thus, when colon cancer cells were excluded, F-18-FDG uptake showed higher correlation with total hexokinase and mitochondrial hexokinase activity (r=0.81, p=0.0027 and r=0.81, p=0.0049, respectively). Conclusion: Both Glut-1 expression and hexokinase activity were contributing factors related to F-18-FDG accumulation in human cancer cell lines. The relative contribution of Glut-1 expression and hexokinase activity, however, was different among different cancer cell types.

• The Korean Journal of Nuclear Medicine
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• v.34 no.4
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• pp.344-352
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• 2000

Purpose: Re-188-Hydroxyethylidene diphosphonate (HEDP) is a new cost-effective agent for systemic radioisotope therapy of metastatic bone pain. We investigated the influence of carrier for labeling and biodistribution of Re-188-HEDP using HEDP kit with or without carrier ($KReO_4$). Materials and Methods: The kits (HEDP 15 mg, gentisic acid 4 mg and $SnCl_2.2H_2O_2$ 4.5 mg) with or without carrier ($KReO_4$ 0.1 mg) were labeled with Re-188 solution, made available from an in-house generator by boiling for 15 min. We compared the labeling efficiency and stability of carrier-added and carrier-free preparations of Re-188-HEDP Biodistribution and imaging studies of each preparation were performed in ICR mice ($1.85{\sim}3.7MBq/0.1ml$) and SD rats ($74.1{\sim}85.2MBq/0.5ml$). Results: The carrier-added preparation showed high labeling efficiency (95% at pH 5) and high stability in serum (88%, 3 hr). However, the carrier-free preparation showed low labeling efficiency (59% at pH 5) and low stability (43%, 3 hr). The carrier-added preparation showed high uptake in bone and low uptake in stomach and kidneys. However, the carrier-free preparation showed lower uptake in bone and higher uptake in both stomach and kidneys, which is supposed to be due to released perrhenate. The carrier-added preparation also showed better images with higher skeletal accumulation, lower uptake in other organs and lower soft tissue uptake than the carrier-free preparation. Conclusion: The results of these studies clearly demonstrate that addition of carrier perrhenate is required for high labeling efficiency, stability, bone uptake and good image quality of Re-188-HEDP.