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http://dx.doi.org/10.3839/jabc.2021.028

Optimization of the extraction procedure for quantitative analysis of saponarin and the artificial light condition for saponarin production from barley sprout  

Oh, Kyeong-Yeol (Department of Agricultural Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University)
Song, Yeong Hun (Department of Agricultural Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University)
Lee, Duek-Yeong (Department of Agricultural Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University)
Lee, Tae-Geun (Department of Agricultural Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University)
Kim, Jin-Hyo (Department of Agricultural Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University)
Publication Information
Journal of Applied Biological Chemistry / v.64, no.3, 2021 , pp. 197-203 More about this Journal
Abstract
Saponarin is a crucial component of barley sprout, and the production and quantitative analysis are issued to date. In this study, the optimal saponarin extraction conditions were presented on the subject of acetonitrile, ethanol, methanol, and water for the quantitative analysis in barley sprout through the extraction efficiency compared with the solvent concentration and extraction time using the reaction surface methodology. The optimal extraction time and solvent condition for saponarin were 3.9 h and 53.7% of aqueous methanol, respectively. In addition, the effect of LED artificial light on the saponarin production in barley sprouts was evaluated by the light cycle, light quantity, and light quality. The optimal cultivation conditions under artificial light for the growth of barley sprout and saponarin production were most effectively achieved on 220-320 μmol m-2 s-1 of the light quantity with 8 h day-1 of a daylight cycle under 6500K LED combined with red light. Furthermore, blue light was evaluated as the main factor in the biosynthesis of saponarin.
Keywords
Artificial light; Barley sprout; Extraction method; Optimization; Saponarin;
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1 Basile A, Giordano S, Lopez-Saez JA, Cobianchi RC (1999) Antibacterial activity of pure flavonoids isolated from mosses. Phytochemistry 52: 1479-1482. doi: 10.1016/S0031-9422(99)00286-1   DOI
2 Park S, Cho E, An J, Yoon B, Choi K, Choi E (2019) Plant growth and ascorbic acid content of spinacia oleracea grown under different light-emitting diodes and ultraviolet radiation light of plant factory system. J Bio-Env Con 28: 1-8. doi: 10.12791/KSBEC.2019.28.1.1   DOI
3 Tetko IV, Gasteiger J, Todeschini R, Mauri A, Livingstone D, Ertl P, Palyulin VA, Radchenko EV, Zefirov NS, Makarenko AS, Tanchuk VY, Prokopenko VV (2005) Virtual computational chemistry laboratory - Design and description, ALOGPS (Version 2.1) [Computer Software]. J Comput Aided Mol Des 19: 453-463   DOI
4 Gomes PGC, Veloso AF, Maynard IFN, Marques MN, de Souza RL, Pereira MM, Soares CMF, Lima AS (2020) Integrative process to extract chlorophyll and purify rosmarinic acid from rosemary leaves (Rosmarinus officialis). J Chem Technol Biotechnol 95: 1503-1510. doi: 10.1002/jctb.6343   DOI
5 Butler WL, Hendricks SB, Siegelman HW (1964) Action spectra of phytochrome in vitro. Photochem Photobiol 3: 521-528. doi: 10.1111/j.1751-1097.1964.tb08171.x   DOI
6 Kozai T, Kubota C, Chun C, Afreen F, Ohyama K (2000) Necessity and concept of the closed transplant production system. In: Kubota C, Chun C (eds) Transplant production in the 21st century. Springer, Dordrecht, pp 3-19
7 Neff MM, Van Volkenburgh E (1994) Light-stimulated cotyledon expansion in Arabidopsis seedlings (the role of phytochrome B). Plant Physiol 104: 1027-1032. doi: 10.1104/pp.104.3.1027   DOI
8 Quail PH (1991) Phytochrome: A light-activated molecular switch that regulates plant gene expression. Annu Rev Genet 25: 389-409. doi: 10.1146/annurev.ge.25.120191.002133   DOI
9 Meng XC, Xing T, Wang XJ (2004) The role of light in the regulation of anthocyanin accumulation in Gerbera hybrida. J Plant Growth Regul 44: 243-250. doi: 10.1007/s10725-004-4454-6   DOI
10 Kim S, Bok G, Park J (2018) Analysis of antioxidant content and growth of Agastache rugosa as Affected by LED light qualities. J Bio-Env Con 27: 260-268. doi: 10.12791/KSBEC.2018.27.3.260   DOI
11 Kim KT, Seog HM, Lee SH, Kim DM (2003) The functionality of barley leaves and its application on functional foods. Food Sci Ind 36: 45-49
12 Benedet JA, Umeda H, Shibamoto T (2007) Antioxidant activity of flavonoids isolated from young green barley leaves toward biological lipid samples. J Agric Food Chem 55: 5499-5504. doi: 10.1021/jf070543t4.   DOI
13 Ha KY (2018) Current status on research and industrial value of balrey sprout. Dissertation, Kongju National University
14 Chung SO, Kim YM, Ryu DG, Kim SJ, Park JT (2014) Variation of functional compounds in leafy chinese cabbage grown under different light conditions in a plant factory. Korean J Food Sci Technol 46: 526-529. doi: 10.9721/KJFST.2014.46.4.526   DOI
15 Hughes KW (1981) In vitro ecology: Exogenous factors affecting growth and morphogenesis in plant culture systems. Environ Exp Bot 21: 281-288. doi: 10.1016/0098-8472(81)90038-1   DOI
16 Kim JS (2021) The effect of artificaial lights on the growth and quality of hydroponic cultivated barley (Hordeum vulgare L.) sprouts. J Plant Biotechnol 48: 62-70. doi: 10.5010/JPB.2021.48.1.062   DOI
17 Gupta SD, Jatothu B (2013) Fundamentals and applications of lightemitting diodes LEDs in in vitro plant growth and morphogenesis. Plant Biotechnol Rep 7: 211-220. doi: 10.1007/s11816-013-0277-0   DOI
18 Lee JJ, Park DH, Lee WY (2017) Optimization of microwave assisted extraction process of Hordeum vulgare L. by response surface methodology. Korean J Food Preserv 24: 949-956. doi: 10.11002/kjfp.2017.24.7.949   DOI
19 Um YC, Oh SS, Lee JG, Kim SY, Jang YA (2010) The development of container-type plant factory and growth of leafy vegetables as affected by different light sources. J Bio-Env Con 19: 333-342
20 Lee GI, Kim HJ, Kim SJ, Lee JW, Park JS (2016) Increased growth by LED and accumulation of functional materials by fluorescence lamps in a hydroponics culture system for Angelica gigas. J Bio-Env Con 25: 42-48. doi: 10.12791/KSBEC.2016.25.1.42   DOI
21 Lee S, Park S (2014) LED array design for optimal combination of plant grown. J Plant Biotechnol 41: 123-126. doi: 10.5010/JPB.2014.41.3.123   DOI
22 Lee HR, Kim HM, Kim HM, Park SH, Hwang SJ (2019) Applicability of artificial light source and newly developed growing medium for lettuce cultivation in a closed-type plant production system. J Bio-Env Con 28: 134-142. doi: 10.12791/KSBEC.2019.28.2.134   DOI
23 Park SY, Oh MM (2021) Enhancement of crepidiastrum denticulatum production using supplemental far-red radiation under various white LED lights. J Bio-Env Con, 30: 149-156. doi: 10.12791/KSBEC.2021.30.2.149   DOI
24 Bula RJ, Morrow RC, Tibbitts TW, Barta DJ, Ignatius RW, Martin TS (1991) Light-emitting diodes as a radiation source for plants. Hortscience 26: 203-205. doi: 10.21273/HORTSCI.26.2.203   DOI
25 Kim S, Bok G, Lee G, Park J (2017) Growth characteristics of lettuce under different frequency of pulse lighting and RGB ratio of LEDs. J Bio-Env Con 26: 123-132. doi: 10.12791/KSBEC.2017.26.2.123   DOI
26 Kang S, Yang HJ, Ko BS, Kim MJ, Kim BS, Park S (2015) Effect of LED with mixed wavelengths on bio-active compounds in cherry tomato and red cabbage. Korean J Food Cook Sci 31: 505-509. doi: 10.9724/kfcs.2015.31.4.505   DOI
27 Lee YH, Kim JH, Kim SH, Oh JY, Seo WD, Kim KM, Jung JC, Jung YS (2016) Barley sprouts extract attenuates alcoholic fatty liver injury in mice by reducing inflammatory response. Nutrients 8: 440. doi: 10.3390/ nu8070440   DOI
28 Yeh H, Chung JP. (2009) High-brightness LEDs-energy efficient lighting sources and their potential in indoor plant cultivation. Renew Sust Energ Rev 13:2175-2180. doi: 10.1016/j.rser.2009.01.027   DOI
29 Zheng L, Van Labeke MC (2017) Chrysanthemum morphology, photosynthetic efficiency and antioxidant capacity are differentially modified by light quality. Plant Physiol 213: 66-74. doi: 10.1016/j.jplph.2017.03.005   DOI