• Title/Summary/Keyword: 변이분석

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Development of Salt-Tolerant Transgenic Rice Using Soybean PR10 Gene (콩의 Pathogenesis-Related 10 유전자를 이용한 내염성 벼 형질전환 계통 개발)

  • Kim, Hyo Jin;Baek, So Hyeon;Shin, Woon Chul;Seo, Chun Sun;Park, Myoung Ryoul;Ko, Jae Kwon;Yun, Song Joong
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.540-546
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    • 2010
  • This study was conducted to understand the role of soybean pathogenesis-related 10 (GmPR10) gene in salt tolerance and to develop salt-tolerant rice using GmPR10 cDNA. GmPR10 transgene was expressed constitutively in the shoot and root of the $T_1$ transgenic rice plants. Interestingly, however, the levels of the transgene expression were increased temporally up to over four- to five-fold in the shoot and root by 125 mM NaCl treatment, peaking at six hours after the treatment and decreasing thereafter. Electrolyte leakage of leaf cells under 125 mM NaCl treatment was lower in all the transgenic lines than in the control variety, Dongjin-byeo. Ability of seedlings to recover from 125 mM NaCl treatment for two weeks was higher in the transgenic plants than in the control plants. These results demonstrated that GmPR10 had function to increase cell integrity and promote growth under the saline stress imposed by NaCl. The transgenic line GmPR10-3 which showed highest ability to recover from the saline stress could be used as a potential source for salt tolerance in rice breeding programs.

Identification of a Major QTL, qSTV11SG, Associated with Resistance to Rice Stripe Virus Disease Originated from Shingwangbyeo in Rice (Oryza Sativa L.) (신광벼 유래의 벼 줄무늬잎마름병 저항성 주동 QTL qSTV11SG탐색)

  • Kwak, Do-Yeon;Lee, Bong-Chun;Choi, Ilyoung;Yeo, Un-Sang;Cho, Jun-Hyun;Lee, Ji-Yoon;Song, You-Chun;Yun, Yeong-Nam;Park, Dong-Soo;Kang, Hang-Won;Nam, Min-Hee;Lee, Jong-Hee
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.464-469
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    • 2011
  • Virus diseases often cause serious damage to rice production in Asia. The lack of information on virus resistance genes has been a major obstacle for the breeding of resistant varieties. In order to identify DNA marker associated with resistance against rice stripe virus (RSV), the quantitative trait locus (QTL) was carried out using advanced backcross population developed from a cross between RSV-resistant tongil type cultivar Shinkwang and susceptible japonica cultivar Ilpum. A RSV resistance QTL $qSTV11^{SG}$ explaining 44.2% of the phenotypic variation was identified on chromosome 11 of Tongil type rice cultivar 'Shingwang'. $qSTV11^{SG}$ was tightly linked to DNA marker RM6897. The RM6897 divided as resistance type allele and susceptible type alleles. Twenty seven resistant varieties showed the resistant-type allele and 23 susceptible varieties were susceptible-type allele to the marker of RM6897. This results and the molecular markers presented here may be useful in rice breeding for improving RSV resistance in japonica rice.

Shelter location-allocation for Tsunami Using Floating Population and Genetic Algorithm (유동인구 데이터와 유전자 알고리즘을 이용한 지진해일 대피소 선정)

  • Bae, Junsu;Kim, Mi-Kyeong;Yoo, Suhong;Heo, Joon;Sohn, Hong-Gyoo
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.37 no.3
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    • pp.157-165
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    • 2019
  • Recently, large and small earthquakes have occurred in the Korean peninsula. In this sense, Korea is no longer considered as an earthquake free zone. Especially, it is necessary to respond quickly to earthquake tsunami which may be caused by the influence of neighboring countries with large earthquakes. Since the occurrence of tsunamis can cause great casualties, it is very important to allocate the location of the shelter in case of an earthquake. Although many researches on shelter allocation have been conducted in various ways, but most of them have been analyzed based on administrative district resident data, resulting in a lack of reality. In this study, floating population data were used to reflect reality in case of emergency situations, and genetic algorithm, which produce good results among the heuristic algorithms, was used to select shelter locations. The number of evacuees was used as a objective function of genetic algorithm and the optimal solution was found through selection, crossover and mutation. As a result of the research on Busan Haeundae-Gu, selected as a research area, allocating eight shelters was the most efficient. The location of the new shelters was selected not only in residential areas but also in major tourist areas whose results can not be derived from administrative district resident data alone, and the importance of utilizing the floating population data was confirmed through this study.

A Study on the Documentation Method of Intangible Cultural Heritage and Training Center (무형문화유산 기록화와 전수교육관에 대한 연구)

  • Kim, Ji-hee;Lee, Young-hak
    • The Korean Journal of Archival Studies
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    • no.56
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    • pp.147-182
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    • 2018
  • Intangible cultural heritage is formless. It's of importance to preserve heritage in line with society drastically being transformed. It's passed down by human being. It is, on the other hand, impossible to preserve intangible cultural heritage solely based on memory, for it's changed in succession. Thus, it be archived to pass down. This study investigated on the state of affair related with Intangible cultural heritage documentations, examined the shortages thereupon. Furthermore, Training center should be in charge of documentation for a solution. This will provide an opportunity to overcome the limitations attributed to the top-down documentation system by central administrative organ, to open up the possibility of viewpoint of different successors, organizations. In addition to that, Intangible cultural heritage documents should be of different aspects of community history. Features of Intangible cultural heritages, which are convergent to the Regionality, tendency of variation, transmission, can be magnified when documentating. Finally, Training center is going to play a major role being vitalised. The record management of intangible cultural heritage, institutions and policies about Intangible cultural heritage have been highly complicated since National Intangible Heritage Center opened and the law on Intangible cultural heritage enacted with its recognition attracted. Therewith this study wishes to see a cornerstone of records on intangible cultural heritage by laying stress on training center in which educations and pass-down of intangible cultural heritage are lively achieved.

The use of MODIS atmospheric products to estimate cooling degree days at weather stations in South and North Korea (MODIS 대기자료를 활용한 남북한 기상관측소에서의 냉방도일 추정)

  • Yoo, Byoung Hyun;Kim, Kwang Soo;Lee, Jihye
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.2
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    • pp.97-109
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    • 2019
  • Degree days have been determined using temperature data measured at nearby weather stations to a site of interest to produce information for supporting decision-making on agricultural production. Alternatively, the data products of Moderate Resolution Imaging Spectroradiometer (MODIS) can be used for estimation of degree days in a given region, e.g., Korean Peninsula. The objective of this study was to develop a simple tool for processing the MODIS product for estimating cooling degree days (CDD), which would help assessment of heat stress conditions for a crop as well as energy requirement for greenhouses. A set of scripts written in R was implemented to obtain temperature profile data for the region of interest. These scripts had functionalities for processing spatial data, which include reprojection, mosaicking, and cropping. A module to extract air temperature at the surface pressure level was also developed using R extension packages such as rgdal and RcppArmadillo. Random forest (RF) models, which estimate mean temperature and CDD with a different set of MODIS data, were trained at 34 sites in South Korea during 2009 - 2018. Then, the values of CDD were calculated over Korean peninsula during the same period using those RF models. It was found that the CDD estimates using the MODIS data explained >74% of the variation in the CDD measurements at the weather stations in North Korea as well as South Korea. These results indicate that temperature data derived from the MODIS atmospheric products would be useful for reliable estimation of CDD. Our results also suggest that the MODIS data can be used for preparation of weather input data for other temperature-based agro-ecological models such as growing degree days or chill units.

The Study of Correlation Between the Balance, Cognition and Activity of Daily Living in Stroke Patients (뇌졸중 환자의 균형, 인지, 일상생활 평가의 상관성 연구)

  • Kang, Bo-Ra;Jeong, Eun-Song;Kim, Jae-Hee;Ha, Yoo-Na
    • Journal of Korean Society of Neurocognitive Rehabilitation
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    • v.10 no.2
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    • pp.45-52
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    • 2018
  • The purpose of the present study was to determine correlations between the Berg Balance Scale (BBS), Montreal Cognitive Assessment-Korean (MoCA-K) and Modified Barthel Index (MBI) targeting stroke patients, and it seeks to analyze the influence among each factor to establish the fundamental research in evaluating the functional performance capability of stroke patients. The study was conducted between December 2017 and March 2018 and the target of the study was 34 stroke patients who are hospitalized and treated in Y rehabilitation hospital located in Goyang city. Following in criteria of how participants were selected. First, a person without the onset of 6months or more. Second, a person who can communicate and score over 20 points on MMSE-K. Third, a person without unilateral neglect. Fourth, a person without lower motor neuron lesion and orthopedic disease on the bilateral lower extremity. Fifth, a person without audiovisual problem and history of using drug or surgery that influence athletic function. sixth, patients who agreed on participating in the study. The evaluation was processed by measuring BBS, MoCA-K, and MBI with the occupational therapist and physical therapist. Also, one assistant was participated in measuring balanced ability for the safety reason. It was found that significantly correlates (p<.01) with BBS and MoCA-K (r=.459), BBS and MBI (r=.550), MoCA-K and MBI (r=.565). This study is meaningful that it provided the basis for the active use of BBS, MoCA-K and MBI as a clinical evaluation tool and its usefulness.

Critical Low Temperature and Response of Behavioral Tolerance in Red Seabream Pagrus major fingerlings Exposed to Cold Shock (저온 충격에 노출된 참돔 Pagrus major 치어의 임계 저 수온 및 행동 내성 반응)

  • Yoon, Sung Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.1
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    • pp.575-584
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    • 2021
  • The critical low temperature and response of the behavioral tolerance of red sea bream Pagrus major fingerlings were determined using the continuous behavior monitoring system (CBMS). The behavior of the experimental organisms was observed by decreasing the water temperature by 2.0℃ and 4.0℃ every 12 hours and 24 hours in the range of 8.0-20.0℃. An unstable behavior pattern was observed in red seabream fingerlings exposed to water temperatures below 12.0℃, in which the swimming activity decreased and repeatedly stopped, regardless of the exposure time and water temperature fluctuation. The swimming ability of the organisms exposed to 8.0-10.0℃ decreased sharply, and the behavior of staying at the bottom of the test tank was observed. Only 50 % of the organisms survived due to the low-temperature stress, and all individuals died within six hours after the cold shock. In addition, the behavior index (BI) decreased rapidly, and the amplitude change of the coefficient of variation (CV) was found to have a greater variation than the other water temperatures (p<0.05). Low-temperature stress of red sea bream is promoted at 12.0℃, and it is interpreted as the tolerance limit, which can induce a sublethal response of the organisms exposed to cold shock of 8.0-10.0℃.

Characterization and Phenolic Compound Analysis of Cheorwon Onion by Lime-sulphur Mixture Treatment (석회유황합제 처리에 따른 철원 양파의 생육특성 및 페놀화합물 분석)

  • Kim, Y.B.;Lee, H.J.;Kim, D.H.;Koo, H.J.;Chang, K.J.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.22 no.1
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    • pp.15-25
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    • 2020
  • The aim of this study was to evaluate the change of phenolic compounds after lime-sulphur mixture treatment on Cheorwon onion. Onion is a perennial plant belonging to the lily family. It is native to Persia of Southwest Asia. It is cultivated much in the temperate regions of the world. Onion is a good name for the 'Okchong' to drop blood cholesterol and cardiovascular blood flow to increase the prevention of adult diseases. Cheorwon area is inland, but it has high continental climate due to its high altitude. After the treatment with lime-sulphur mixture, the weight, width and plant height of the onion were not affected. In the phenolic compounds, caffeic acid, ferulic acid, rutin, p-coumaric acid and kaempferol except benzoic acid and quercetin were similar to or less than the control. Therefore, it was concluded that the treatment with lime-sulphur mixture had some effect on phenolic compound contents, benzoic acid and qurercetin.

Synthesis of Zn-intermediate from alkali agents and its transformation to ZnO crystallinity (알칼리 침전제에 의해 제조된 아연 중간생성물 및 산화아연 결정화)

  • Jang, Dae-Hwan;Kim, Bo-Ram;Kim, Dae-Weon
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.31 no.6
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    • pp.270-275
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    • 2021
  • ZnO was synthesized according to the transformation behavior and crystallization conditions of Zn-intermediate obtained by zinc sulfate as a precursor and NaOH, Na2CO3 as a alkali agents. For ZnO crystallization, Zn4(OH)6SO4·H2O and Zn5(OH)6(CO3)2·H2O as a Zn-intermediate were calcined at 400℃ and 800℃ for 1 h, respectively, based on decomposition temperature from TGA. Zn4(OH)6SO4·H2O was confirmed to have mixed Zn4(OH)6SO4·H2O and ZnO at 400℃, and was completely thermally decomposed at 800℃ to form ZnO phase. The prepared Zn5(OH)6(CO3)2·H2O as a Zn-intermediate by the reaction with Na2CO3 was transformed to a complete ZnO crystallization over 400℃. Nano-sized ZnO can be synthesized at a relatively lower calcination temperature through the reaction with Na2CO3.

A Study on Stability evaluation in the freezing/thawing process of urine specimen analytes (소변 검체 분석물질의 냉/해동 과정 안정성 평가 연구)

  • Kim, Min Kyung;Kim, Sung Wook;Hwang, You Seong;Oh, Eunha
    • Journal of the Korean Applied Science and Technology
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    • v.39 no.1
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    • pp.52-62
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    • 2022
  • The purpose of this study was to find a way to improve the stability and quality of urinalysis by checking the changes in the measurement values of representative clinical chemistry test items according to the repeated freezing and thawing before the urine test and the thawing process. All subjects were 10 healthy males, and the freeze and thaw stability test was performed using their urine samples. In the case of micro-albumin and amylase, there was no statistical significance at 37℃ with time, but at 42℃ and 60℃, there was a statistically significant change in the results with time. There were statistically significant changes in BUN, creatinine, uric acid, and glucose. As a result of long-term stability, after 7 days, glucose mutation increased and amylase decreased at 60℃. In the case of glucose and amylase, there was a statistically significant change in the results over time. To obtain accurate test results, accurate standardization of urinalysis including appropriate collection, storage, and storage methods of urine samples is required and systematic study of conditions for securing stability for each biomaterial is required.