Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Identification of a Major QTL, qSTV11SG, Associated with Resistance to Rice Stripe Virus Disease Originated from Shingwangbyeo in Rice (Oryza Sativa L.)

신광벼 유래의 벼 줄무늬잎마름병 저항성 주동 QTL qSTV11SG탐색

  • Kwak, Do-Yeon (Department of Functional Crop Science, NICS, RDA) ;
  • Lee, Bong-Chun (National Institute of Crop Science, RDA) ;
  • Choi, Ilyoung (Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute) ;
  • Yeo, Un-Sang (Department of Functional Crop Science, NICS, RDA) ;
  • Cho, Jun-Hyun (Department of Functional Crop Science, NICS, RDA) ;
  • Lee, Ji-Yoon (Department of Functional Crop Science, NICS, RDA) ;
  • Song, You-Chun (Department of Functional Crop Science, NICS, RDA) ;
  • Yun, Yeong-Nam (Department of Functional Crop Science, NICS, RDA) ;
  • Park, Dong-Soo (Department of Functional Crop Science, NICS, RDA) ;
  • Kang, Hang-Won (Department of Functional Crop Science, NICS, RDA) ;
  • Nam, Min-Hee (Department of Functional Crop Science, NICS, RDA) ;
  • Lee, Jong-Hee (Department of Functional Crop Science, NICS, RDA)
  • 곽도연 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 이봉춘 (농촌진흥청 국립식량과학원) ;
  • 최일룡 (국제미작연구소 유전육종과) ;
  • 여운상 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 조준현 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 이지윤 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 송유천 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 윤영남 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 박동수 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 강항원 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 남민희 (농촌진흥청 국립식량과학원 기능성작물부) ;
  • 이종희 (농촌진흥청 국립식량과학원 기능성작물부)
  • Received : 2011.12.14
  • Accepted : 2011.12.19
  • Published : 2011.12.30
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Abstract

Virus diseases often cause serious damage to rice production in Asia. The lack of information on virus resistance genes has been a major obstacle for the breeding of resistant varieties. In order to identify DNA marker associated with resistance against rice stripe virus (RSV), the quantitative trait locus (QTL) was carried out using advanced backcross population developed from a cross between RSV-resistant tongil type cultivar Shinkwang and susceptible japonica cultivar Ilpum. A RSV resistance QTL $qSTV11^{SG}$ explaining 44.2% of the phenotypic variation was identified on chromosome 11 of Tongil type rice cultivar 'Shingwang'. $qSTV11^{SG}$ was tightly linked to DNA marker RM6897. The RM6897 divided as resistance type allele and susceptible type alleles. Twenty seven resistant varieties showed the resistant-type allele and 23 susceptible varieties were susceptible-type allele to the marker of RM6897. This results and the molecular markers presented here may be useful in rice breeding for improving RSV resistance in japonica rice.

벼 줄무늬잎마름병 저항성 유전자 및 연관 DNA 마커 탐색을 위하여 줄무늬잎마름병에 저항성인 통일형 품종인 신광 벼 이용 여교잡 집단을 육성하였다. 줄무늬잎마름병 저항성 유전자에 대한 QTL을 분석한 결과 11번 염색체에 위치하는 SSR 마커 RM6897이 탐색되었으며 전체 표현형 변이의 44.2%를 설명하였다. DNA 마커 RM6897은 여교잡 집단에서 생물검정과 유전자형이 일치하였다. 또한 자포니카 품종들에서 저항성 27품종과 감수성 23품종에 대해 구분이 가능하였다. 따라서 신광벼 유래의 줄무늬잎마름병 저항성원 및 분자마커는 자포니카 품종의 바이러스 저항성 향상에 효율적으로 활용될 것으로 기대된다.

Keywords

References

  1. Albar L, Ndjiondjop MN, Esshak Z, Berger A, Pinel A, Jones A, Fargette D. Ghesquière A. 2003. Fine genetic mapping of a gene required for Rice yellow mottle virus cell-to-cell movement. Theor. Appl. Genet. 107:371-37. https://doi.org/10.1007/s00122-003-1258-4
  2. Hayano-Saito Y, Saito K, Nakamura S, Kawasaki S. Iwasaki M. 2000. Fine physical mapping of the rice stripe resistance gene locus, Stvb-i. Theor. Appl. Genet. 101:59-63. https://doi.org/10.1007/s001220051449
  3. Hayashi K, Yoshida H, Ashikawa I. 2006 Development of PCR-based allele specific and InDel marker sets for nine rice blast resistance genes. Theor. Appl. Genet. 113: 251-60. https://doi.org/10.1007/s00122-006-0290-6
  4. Hibino H. 1996. Biology and epidemiology of rice viruses. Annu. Rev. Phytopathol. 34:249-274. https://doi.org/10.1146/annurev.phyto.34.1.249
  5. Kwak DY, Yeo US, Lee JH, Park DS, Song YC, Kim CS, Oh BG, Shin MS, Ku YC. 2007. Gene introgression effects of green rice leafhopper resistance on grain quality in rice Korean J. Breed. Sci. 39:485-490.
  6. Lee BC, Yoon YN, Hong SJ, Hong YK, Kwak DY, Lee JH, Yao US, Kang HW, Hwang HG. 2008. Analysis on the occurrence of rice stripe virus. Res. Plant Dis. 14:210- 213. https://doi.org/10.5423/RPD.2008.14.3.210
  7. Lee JH, Kwak DY, Ye o US, Kim CS, Joen MG, Kang JR, Park DS, Shin MS, Oh BG, Hwang HG. 2008. Modified CTAB DNA methods for efficient DNA extraction from rice (Oryza sativa L.). Korean J. Breed. Sci. 40: 286-290.
  8. Ling K.C. 1975. Rice virus diseases. IRRI 142p.
  9. Maeda H, Sugisawa T, Nemoto H, Sunohar Y. 2004. QTL analysis for rice stripe resistance in the japanese upland rice Kanto 72. Breeding Sci. 54:19-26. https://doi.org/10.1270/jsbbs.54.19
  10. McCouch, S.R., Teytelman, T., Xu, Y., Lobos, K.B., Clare, K., Walton, M. 2002. Development and mapping of 2240 new SSR markers for rice. DNA research 9:199- 207. https://doi.org/10.1093/dnares/9.6.199
  11. Suh JP, Roh JH, Cho YC, Han SS, Kim YG, Jena KK. 2009. The pi40 gene for durable resistance to rice blast and molecular analysis of pi40-advanced backcross breeding lines. Phytopathology. 99:243-250. https://doi.org/10.1094/PHYTO-99-3-0243
  12. Sun X, Cao Y, Yang Z, Xu C, Li X, Wang S, Zhang Q (2004) Xa26, a gene conferring resistance to Xanthomonas oryzae pv. oryzae in rice, encoding a LRR receptor kinase-like protein. Plant J 37:517-527. https://doi.org/10.1046/j.1365-313X.2003.01976.x
  13. Washio O, Ezuka A, Sakurai Y, Toriyama K (1968) Studies on the breeding of rice varieties resistant to rice stripe disease. III. Genetic studies on resistance to stripe in foreign varieties. Jpn J Breed 18:167-172.