• Title/Summary/Keyword: 변이분석

Search Result 2,743, Processing Time 0.043 seconds

A Study on the Strategy to promote Geospatial Information Industry based on Users Requirements Analysis (수요자 요구 분석을 통한 공간정보산업 진흥 전략에 관한 연구 -전문가 설문조사를 중심으로-)

  • Seok, Sangmuk;Song, Ki Sung;Hwang, Jung Rae
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.17 no.7
    • /
    • pp.400-409
    • /
    • 2016
  • The geospatial information industry has the advantage of being able to converge with other industries to create new value. Therefore, efforts are underway to promote the growth of this dwindling national industry by endorsing policies and initiatives to promote its convergence with other industries. In this study, feedback was collected from experts in various fields through three rounds of polls using the Delphi method. The poll results were used to investigate the causes of hindrance to the growth of the geospatial information industry and to build a comprehensive solution to this problem. As a result, 27 strategies were constructed based on the users' requirements related to policy, data and service, including the demands for establishing public polling methods, enhancing data quality and advertisement. Since the coefficient of variance fell between 0.11 and 0.20 in the importance evaluation, an amicable consensus was reached between the experts. Considering that the convergence of industries is achieved through the integration of various types of technology and information, it is important to consider the requirements of users in different industries. In this respect, the results of this study will serve as a useful base for constructing mid-to-long term road maps to promote the development of the geospatial information industry.

Effects of Residual Solvents in the Phase Transition, Transition Enthalpy, and Transition Temperature of Phospholipid Membranes (잔류 유기 용매가 모델 세포 지질막의 상전이, 상전이 엔탈피 및 상전이 온도에 미치는 영향)

  • An, Eun Seol;Choi, Jae Sun;Lee, Dong Kuk
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.40 no.2
    • /
    • pp.163-170
    • /
    • 2014
  • Lipid membranes composed of phosphatidylcholine (PC) are used in biophysical study to mimic cellular membranes and interactions between the membrane and chemicals, where organics solvents are used in dissolving lipids or chemicals. Later, solvents are removed from the solution under nitrogen gas at room temperature, followed by the further removal of the solvent at vacuum condition for several hours. In this process, some solvents are easily removed under described conditions above and others are required more severe conditions. In this study, $^{31}P$ solid-state nuclear magnetic resonance (SSNMR) techniques and differential scanning calorimetry (DSC) were used to see any changes in the line shapes of $^{31}P$ NMR spectra of multilamellar vesicles (MLVs) samples of POPC and in the phase change temperature of multilamellar vesicles (MLVs) of DPPC in DSC thermogram with or without any residual solvents. The thermodynamic parameters associated with the solvents did exhibit noticeable changes depending on solvent types. Thus, it is concluded that solvents should be carefully chosen and removed completely and experimental results should also be interpreted with caution particularly for the experiments investigating lipid phase changes and related topics.

Changes in the Polypeptide Patterns of Oat Root Tips Exposed to Alachlor (Alachlor에 의한 귀리 근단(根端) 분열조직(分裂組織)의 단백질(蛋白質) Pattern의 변이(變異))

  • Kwon, S.W.;Park, K.I.;Kim, J.C.
    • Korean Journal of Weed Science
    • /
    • v.12 no.4
    • /
    • pp.368-373
    • /
    • 1992
  • The effect of alachlor treatment on protein synthesis was studied. Protein synthesis was inhibited by $1{\times}10^{-4}$ M and $1{\times}10^{-3}$M of alachlor 5.8% and 86.5%, respectively, while did not occur blow $1{\times}10^{-5}$M alachlor. Soluble protein of alachlor treated oat root tips was examined by polyacrylamide gel electrophoresis. The proteins extracted from oat root tips showed that they were made up of subunits blow 100 kd polypeptides by SDS-PAGE. As compared to control, high molecular proteins(above 47 kd) were inhibited of oat root treated with alachlor, while low molecular proteins(below 23 kd) were increased. Two-D gels showed that alachlor caused decrease(1-6 spots) or increase(7-10 spots) in number of polypeptides on silver staining. The intensity of some polypeptides of soluble proteins (molecular mass of 83 kd : 1, 2 spots, 70 kd : 3, 4 spots, and 47.5 kd : 5, 6 spots) decreased in alachlor treatment, whereas the intensity of other peptide bands (20 kd : 7 spot and 16 kd : 8, 9, 10 spots) increased. Oat root tip proteins present in the neutral zone are masked by diffusing of major proteins, but proteins in acid zone are resolved minor proteins.

  • PDF

Distribution and Management of Naturalized Plants in the Eastern Area of Jeollanamdo, Korea (전라남도 동부지역의 귀화식물 분포 및 관리방안)

  • Park, Moon-Su;Lim, Dong-Ok;Kim, Ha-Song
    • Korean Journal of Plant Resources
    • /
    • v.24 no.5
    • /
    • pp.489-498
    • /
    • 2011
  • This study was carried out to investigate the distribution status and urbanization index of the naturalized plants. For this purpose, 7 cities and counties in the eastern area of Jeollanamdo were selected and investigations were conducted on a total of 14 sites, during the period of March 2006 to November 2008. The naturalized plants were identified as 123; 22 families, 75 genera, 118 species, and 5 varieties. As for the urbanization index, Suncheon city had the highest level of urbanization (33.45%) and Gurye county the lowest level of urbanization (20.21%). Classification families were as follows: Compositae 40 kinds (30.1%) Gramineae 17 kinds (12.8%) and Leguminosae 14 kinds (10.5%). Major communities of naturalized plants consisted of 20 communities including Erigeron annuus community, Erigeron canadensis community, Alnus firma community, Amorpha fruticosa community, Oenothera odorata community, Bidens frondosa community, Coreopsis tinctoria community, Ambrosia artemisiifolia var. elatior community etc. Invasive naturalized plants consisted of 5 taxa: Ambrosia artemisiifolia var. elatior, Ambrosia trifida, Eupatorium rugosum, Paspalum distichum var. indutum and Paspalum distichum. The distributional characteristics of naturalized plants were divided on the basis of six areas associated with ecological characteristics of a habitat.

Dna2 Helicase/endonuclease Interacts with a Novel Protein YHR122W Protein in Saccharomyces cerevisiae (Saccharomyces cerevisiae에서 Dna2 helicase/endonuclease와 YHR122W 단백질의 상호작용)

  • Lee, Hyun-Sun;Choi, Do-Hee;Kwon, Sung-Hoon;Kim, Na-Yeon;Lee, In-Hwan;Kim, Hyun-Jung;Bae, Sung-Ho
    • Korean Journal of Microbiology
    • /
    • v.42 no.1
    • /
    • pp.1-6
    • /
    • 2006
  • Saccharomyces cerevisiae Dna2 helicase/endonuclease plays an essential role in removing DNA primers during Okazaki fragment processing in eukaryotic DNA replication. Genome-wide scale co-immunoprecipitation experiments predicted that Dna2 interacts with a novel protein YHR122W (1). In this study, we observed that overexpression of YHR122W gene suppressed the temperature-sensitive phenotype of $dna2\Delta405N$ mutation. To investigate direct interaction between these two proteins, a histidine-tagged recombinant YHR122W protein was expressed and purified from E. coli. Physical interaction between the purified YHR122W and Dna2 proteins was detected by enzyme-linked immunosorbent assays. Further more, the complex formation was most efficient at physiological salt concentration, 150 mM NaCl. The genetic and physical interactions between YHR122W and Dna2 shown in this study suggest that the biological functions of these two proteins may be closely related each other.

MICROSATELLITE INSTABILITY AND p53 GENE MUTATION IN ORAL SQUAMOUS CELL CARCINOMA (구강편평상피세포암 조직에서의 Microsatellite 불안정성 및 p53 유전자 돌연변이 분석)

  • Choi, Tae-Ho;Chung, Woon-Bok;Hong, Su-Hyung;Kim, Jin-A;Na, Sun-Oung;Jang, Hyun-Jung;Sohn, Yoon-Kyung;Kim, Chin-Soo;Kim, Jung-Wan
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
    • /
    • v.26 no.4
    • /
    • pp.337-344
    • /
    • 2000
  • Germ-line mutations at DNA repair loci confer susceptibility to colon cancer in hereditary non-polypopsis colorectal cancer. Somatic loss of DNA mismatch repair gene has been reported in a large variety of other tumor types. Replication errors(RERs) judged by microsatellite instability(MSI) and its associated mutations have been recognized as an important mechanism in various tumor types. To investigate associations between MSI and oral squamous cell carcinoma, the frequency of MSI using 12 microsatellite markers were analyzed for the series of oral tumors. Of 17 tumors, 8 cases(47%) did not show instability at any of the 12 loci; 5(29%) showed instability at $2{\sim}3$ loci; and 4(24%) showed instability above 4 loci. The 4 cases showing widespread MSI did not differ from those without evidence of instability in terms of age at diagnosis, degree of differentiation, metastasis to lymph node, tumor location or the presence of mutations in the p53 tumor suppressor gene. DCC and D17S 796 were the most frequently detected in MSI analysis. There were no correlation between smoking and MSI frequency, instead, smoking was suggested to increase the mutation rate of p53 and development of oral carcinomas.

  • PDF

Discriminant Analysis of Natural Landscape Features in National Parks between Korea and Scotland - Using Low-Level Functions of Content-Based Image Retrieval - (한국과 영국 사이의 국립공원 자연 경관 특색의 판별 분석 - 내용기반 영상검색의 저단계 기능 측면에서 -)

  • Lee, Duk-Jae
    • Korean Journal of Environment and Ecology
    • /
    • v.22 no.3
    • /
    • pp.289-300
    • /
    • 2008
  • This study aims to discriminate differences in natural landscapes between the Cairngorms National Park in Scotland and the Jirisan National Park in Korea, using functions of content-based image retrieval such as texture, shape, and color. Digital photographs of each National Park were taken and selected. The low-level functions of photographic images were reduced to orthogonally rotated five factors. Based on the reduced factors, a linear decision boundary was obtained between Cairngorms landscapes and Jirisan landscapes. As a result, the discriminant function significantly delineated two groups, resulting in $x^2=63.40$ with df=5(p<0.001). Both the eigenvalue 2.417 and the value of wilks' lambda 0.29 supported that the most proportion of total variability came from the differences between the means of discriminant function of groups. It was estimated that four independent variables explained about 70.7% of total variance of dependent variable. The variable with the largest effect on landscapes was far region-related factor(r=1.07), followed by near region-related factor (r=0.90). A total of 90.7% of cross-validated grouped cases were correctly classified. It was interpreted that far distant regions, as well as near distant regions, had sufficient discrimination power for landscape classification between the Cairngorms National Park and the Jirisan National Park, so that landscape identity of the National Park over cultures was revealed by skylines in a most effective way. Relatively fewer factors making visual landscapes were effectively used to classify natural landscapes of the National Parks which had different semantics.

Head Capsule Width and Population Densities of Overwintering Nymphal Stages of the Green Rice Leafhopper, Nephotettix cincticeps Uhler (Hemiptera: Cicadellidae) (월동 끝동매미충(Nephotettix cincticeps)의 약충태별 두폭크기 및 밀도변화)

  • Kang, Seok-Min;Paik, Chae-Hoon;Lee, Geon-Hwi;Choi, Man-Young;Sengottayan, Senthil-Nathan;Hwang, Chang-Yeon
    • Korean journal of applied entomology
    • /
    • v.48 no.2
    • /
    • pp.165-170
    • /
    • 2009
  • This study was conducted to investigate specific stages and distribution of overwintering nymphs of green rice leafhopper, Nephotettix cincticeps by measuring head capsule width. The nymphal head capsule width of the 1st instar to the 5th instar was 0.381, 0.502, 0.673, 0.979 and 1.128 mm, respectively. lts coefficient variation was 5.3, 4.0, 3.0, 4.5 and 5.3%, respectively. Growth ratio of each instar was not significantly different among 2nd to 4th instars as 1.31 to 1.34, but for the 5th instar it decreased as 1.28. The logarithm of the nymphal head capsule width was regressed as a function of the stage number of insects, resulting in LogY = 1.4627 + 0.1192X ($r^2= 0.9993$). Also fitness to the Dyar's law for the nymphal head capsule width of each instar was 98% or over and the Dyar's constant (K) was 1.316. The occurrence of N. cincticeps was maximum at the end of January with 195 individuals/0.25 $m^2$. The most abundant instar in the overwintering N. cincticeps population was 4th instar accounting for over 90%. Population of the 5th instar began to increase from mid-March, and adults began to occur in early April.

Relationship between Moxifloxacin Resistance Pheno- and Genotype of Moxifloxacin-Resistant Mycoplasma hominis Obtained in vitro (인위적으로 유도된 목시플로사신 내성 Mycoplasma hominis의 표현형과 유전자형의 연관성)

  • Park, In-Dal;Choi, Myung-Won
    • Journal of Life Science
    • /
    • v.20 no.10
    • /
    • pp.1544-1548
    • /
    • 2010
  • Moxifloxacin (MF) - resistant mutants of Mycoplasma hominis (M. hominis) were generated by stepwise selection in increasing concentrations of MF, and six strains of MF resistant M. hominis mutants - M1, M4, M8, M16, M32, and M64 - in which MICs of MF were 0.5, 4, 8, 16, 32, 64 ${\mu}g$/ml, respectively, were generated. Compared to the sequence of M. hominis PG21, all mutants harbored amino acid substitutions of Arg-163 Thr in GyrA, and Pro-445 Gln in ParE. While the concentrations were getting higher, an additional amino acid substitution was found at Ser-153 Lys in GyrA (${\geq}4{\mu}g/ml$), Ser-91 Ile in ParC (${\geq}16{\mu}g/ml$), and Val-450 Phe (${\geq}64{\mu}g/ml$) in GyrB. These substitutions seem to have an impact on resistance to MF, and GyrB change was found only in the highest concentration and seems to be associated with high-level resistance to MF. This, as far as we know, is the first description of a relationship between MF resistance phenotype and genotype.

Limitations of Applying Land-Change Models for REDD Reference Level Setting: A Case Study of Xishuangbanna, Yunnan, China (REDD 기준선 설정 시 토지이용변화 예측모형 적용의 한계: 중국 운남성 시솽반나 열대림 사례를 중심으로)

  • Kim, Oh Seok
    • Journal of the Korean Geographical Society
    • /
    • v.50 no.3
    • /
    • pp.277-287
    • /
    • 2015
  • This paper addresses limitations of land-change modeling application in the context of REDD (Reducing Emissions from Deforestation and forest Degradation). REDD is an international conservation policy that aims to protect forests via carbon credit generation and trading. In REDD, carbon credits are generated only if there is measurable quantied carbon sequestration activities that are additional to business-as-usual (BAU). A "reference level" is defined as simulated baseline carbon emissions for the future under a BAU scenario, and predictive land-change modeling plays an important role in constructing reference levels. It is tested in this research how predictive accuracies of two land-change models, namely Geographic Emission Benchmark (GEB) and GEOMOD, vary with respect to different spatial scales: Xishuangbanna prefecture and Yunnan province. The accuracies are measured by Figure of Merit. In this Chinese case study, it turns out that GEB's better performance is mainly due to quantity (e.g., how many hectares of forest will be converted to agricultural land?) rather than spatial allocation (e.g., where will the conversion happen?). As both quantity and allocation are crucial in REDD reference level setting it appears to be fundamental to systematically analyze accuracies of quantity and allocation independently in pursuit of accurate reference levels.

  • PDF