• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.25-25
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• 2003

본 연구는 돼지 동결-융해 정자의 배양에 의한 체외수정능력과 glycosidase activity의 관계를 검토하였으며, 또한 돼지난자의 투명대내에서 발견된 당잔기에 대한 정자의 glycosidase 특이성을 확인하기 위하여 $\alpha$-L-fucosidase, $\alpha$-D-mannosidase, $\beta$-D-galactosidase 및 N-acetyl-$\beta$-D-glucosaminidase ($\beta$-GlcNAc'ase)의 activity를 분석하였다. 그 결과 glycosidase activity는 동결정자의 융해 후 배양하지 않았을 때보다 2시간 배양했을 때 더 높게 나타났다. $\beta$-GlcNAc'ase의 activity는 정자 배양 유무에 관계없이 다른 glycosidase 처리시보다 최소한 2배 이상 높게 나타났다. 또한 첨체반응이 유기된 정자의 비율은 glycosidase ($\alpha$-D-mannosidase; P<0.05)에 의해 영향을 받았으며 정자를 배양하지 않은 경우보다는 배양된 정자에서 높게 나타났다. 그러나 배양시간에 따른 정자의 생존성에 대해 glycosidase의 종류에 따른 유의차는 인정되지 않았다. 한편 투명대내 정자의 접착과 침입에 대한 또 다른 실험에서, 서로 다른 glycosidase가 첨가된 배양액내에서 수정된 정자가 배양시간이 길어짐에 따라 정자의 침입율은 낮아졌다($\beta$-GlcNAc'ase; P<0.05). 투명대내의 정자접착 정도는 glycosidase의 첨가시에 무첨가시보다 접착정도가 더 높았으며, 가장 높은 접착율은 $\beta$-GlcNAc'ase첨가시 나타났다. 또한 모든 glycosidase 처리시 2시간 배양한 정자보다는 배양하지 않은 정자에서 투명대에 대한 접착정도가 높게 나타났으며, $\alpha$-D-mannosidase의 처리시 유의적인 차이를 보였다(P<0.05). 본 연구의 결과, $\beta$-GlcNAc'ase가 주로 돼지정자의 원형질막내에 존재하는 것으로 추측되며, 배양된 정자에 의한 투명대 접착정도와 침입율이 낮았음에도 불구하고 glycosidase activity가 증가하는 것으로 나타났다

• Journal of Embryo Transfer
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• v.31 no.3
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• pp.273-279
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• 2016

The Korean native cattle, Hanwoo, is the most popular breed of beef cattle in Korea. However, the reproductive performance data are limited although reproduction is one of the most economically and biologically important in beef production. Therefore, this study was undertaken to investigate reproductive performance parameters including calving interval, parity for life time production. Data collected from 206,827 calvings were analyzed. There were no significant differences in calving interval and gestation days as parity increased from 2nd and 13rd parity cow, from spring to winter. However, we found a dramatic increase in calving interval after year 2000. About 1 month were increased per year ( y = 30.578x + 344.45 $R^2=0.9157$). Interestingly, we observed that parities for life time can be affected by birth weight. Calves with 23 kg at birth showed highest parities, $3.4{\pm}2.0$ times. In summary, this study provides valuable data on reproductive performance of Hanwoo and the data presented here can be used as a standard target for optimising and enhancing reproductive performance.

• Korean Journal of Plant Resources
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• v.14 no.1
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• pp.53-59
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• 2001

This study was carried out to investigate mass propagation technique by using seed and vegetative propagation of Kalopanax pictus Nakai. For developing seed propagation, seed stratification and 1$0^{\circ}C$ after-ripening treatment induced embryo growth within 1 weeks, resulted in increasing germination rate of seeds up to more than 65% when planted. The softwood cutting using one year old shoot increased rooting rate to 69% whereas more than 1 year old shoot looked like inappropriate for cutting propagation. In the cutting timing, the rooting rate on June, 13 cutting of the first growth shoot was the highest, followed by June 20 and July 4. The most efficient cutting timing seemed to be the middle of June. When cutted shoots were soaked for 30 minute with IBA and NAA 1000mg.$L^{-1}$, rooting rate was increased above 70%. As the concentrations of plant hormone were increased above 2000mg.$L^{-1}$, the rooting rate was slowly decreased.

• Korean Journal of Animal Reproduction
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• v.25 no.3
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• pp.251-257
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• 2001

This study was carried out to investigate the effect of breed, parity, litter size, lactating period, and estrus interval on sow productivity traits in swine. Data from 492 heads of Landrace, Yorkshire or crossbred sow which were raised at Yonam College of Agriculture from March, 1998 to July, 2000 were analyzed for this study. The total number of pigs born (TN), the number of pigs born alive (NB), and the number of pigs suckled (NS) were greater in the crossbred sows than in the purebred. In TN and NB, the 3rd to 7th parities were greater than other parities, and the 8th parity was the lowest. The number of pigs stillbirthed (NSB) and the number of runt per litter tended to increase with the increase of TN. In addition, statistical analysis showed that parity had significant effect on most of traits. The current TN had highly significant effect on TN, NB and NS of the next parity As current TN increased, TN, NB, and NS of the next parity increased. The current lactating period also affected significantly for TN, NB, and NS of the next parity The sows which had the lactating period of 20∼21 days produced the greatest TN and NB in the next parity. Weaning to estrus interval(WEI) had significant effect on TN, NB, and NSB. Among WEI groups, the WEI group of 7∼13 days was the lowest in TN, NB and NSB.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2011.10a
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• pp.327-330
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• 2011

살아있는 생명체는 세포로 구성되며 성장 분열을 통해 스스로 복제할 수 있는 능력을 지녔다. DNA 상의 변이, 즉 돌연변이는 자손의 생존과 번식에 불리하게 작용할 수 있고 이점을 줄 수 있는 양면성을 지녔다. 본 연구에서는 DNA 이중나선은 복제 주형으로 사용되기 위해서는 먼저 이중나선이 열리고 단일 가닥으로 분리되어야 한다. 이중 나선구조결합에서의 결합의 오류부분의 위치를 찾아내고 복구하는 방법을 제시한다.

• Journal of Embryo Transfer
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• v.9 no.1
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• pp.49-52
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• 1994

This study was carried out to compare farrowing rate and litter traits for European and American lines with boar sperm frozen in straws. Farrowing rate, litter size and mean pig weght at birth and 21 days were investigated. A total of 36 gilts Landrace, Large white and Duroc were investigated at the Chungnam Provincial Animal Breeding Station. We obtained higher farrowing rate and litter traits for European line boars compared to American line boars.

• Journal of the korean veterinary medical association
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• v.36 no.11
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• pp.1021-1028
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• 2000

본 란에서는 지난 호의 군 건강 및 번식 관리 프로그램의 원리에 이어서, 이 프로그램을 효율적인 기록 관리에 대해서 기술하고자 한다. 기록이라 하면 단순한 사실의 나열을 넘어서 자료들을 통합하고 가공하며 유용한 형태로 보관하는 것을 의미한다. 또한 기록은 수의사나 농부의 능력을 가늠하는 척도로 여겨질 만큼 중요하다. 여기서는 무엇을 기록해야 하는가 하는 점에서부터 자료의 저장 방법 및 다양한 시스템들, 특히 컴퓨터 이용의 중요성에 대해서 설명하기로 한다.

• Korean Journal of Animal Reproduction
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• v.24 no.1
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• pp.19-33
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• 2000

Eighty dairy farms (38 farms responded) in Palmerston North area of New Zealand were questioned by mail questionnaire on: 1) general characteristics, 2) milk yield and feed supplementary, 3) reproductive efficiencies (12 questions) and 4) reproductive disorders (12 questions) from February to July, 1998. Among those 4 items, the reproductive efficiencies (3) and disorders (4), various diseases and culling rates were surveyed and analyzed for Korean dairy farmers (especially in Cheju island) and compared with New Zealand. The results are as follows: 1. Fifteen farms in 38 dairy farms relied entirely on artificial insemination, the rest of 23 dairy farms (60.5%) raised 5∼6 bulls to increase conception rate. The dairy farmers in Palmerston North used artificial insemination from Oct 4th to Dec 10th for 42.8 days, and then used bulls from that point to coming Jan 10th for 41.4 days. The submission rate within 3, 6 and 10 weeks following the initiation of AI season was 84.7, 93.9 and 97.9% respectively. 2. The average age of heifers at the first estrus, pregnancy and calving was 11.0, 18.0 and 24.7 months respectively, and an average 1.4 estrus cycles were required for conception. The intervals of estrus recurrence and the following conception after calving were 38 and 68 days respectively. 3. Among inseminated cows, calving, abortion and empty cow was 90.9, 1.6 and 7.4% respectively. Calving rate decreased according to increasing farm size, while the number of empty cows decreased. 4. Stillbirth, retained placenta and delivery abnormalities were 5.3, 3.7 and 5.5% respectively, not different depend on herd size. 5. The incidence of milk fever, grass tetany, and ketosis was 3.6, 3.0 and 1.0%, respectively. The delivery abnormality and mastitis treated with medicine were 3.1 and 6.7%, but decreased according to farm size. Lameness was 8.6% on average, but over 10% in farms which has more than 400 milking cows. 6. Among the culled cows (15.5% of the total), those culled due to an old age, lameness and other diseases were 2.9, 1.8 and 4.3% respectively and those culled due to low milk production, reproductive abnormality reduced with farm size. 7. Compared with the data collected in Korea, the reproductive efficiency was better, and lameness, metabolic problem and culling rate were higher in New Zealand

• Korean Journal of Animal Reproduction
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• v.26 no.2
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• pp.193-199
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• 2002

This study was conducted to investigate the optimal physiologic mating time in Hanwoo for protection to decrease of reproductivity and improvement of production of offspring. We observed 32 cows that were devide into 4 parts of treatment : T1(12 months of age and 0.5kg daily gain), T2(12 months of age and 0.8kg daily gain), T3(15 months of age and 0.5kg daily gain) and T4(18 months and 0.5kg daily gain). The first heat of treated cows was 263.3$\pm$6.4 days and average weight was 181.1$\pm$11.3kg. It was revealed the conception rates of first insemination were 25%(T1), 75%(T4) and number of insemination of T3 and T4(both 1.5) was lower than T1 and T2(2.3 and 2.4). In return of estrus after heifer's first parturition, they(T1, T2, T3 and T4) showed 66.2 days, 76.7 days, 62.4 days and 68.5 days respectively and the average was 65.7 days. Plasma progesterone(P4) concentration was nearly the same during the observation periods of treated cows and P4 was released just after 12 months. Only 5 cows (15.6%) in 32 were showed normal estrus cycle and ovulation before 12 months. Before and after parturition, P4 concentration was decreased fastly and then there was no detection of P4 from after parturition to 40 days after milking. P4 would be released again on 45 day after parturition. The results were summarized as that the optimal mating time of Hanwoo heifers was decided by the 14 months of age, 110 cm height and 265kg weight.

• 월간낙농육우
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• v.36 no.3
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• pp.141-145
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• 2016

봄이 시작되는 3월이다. 봄이 되면 마음이 분주해지고, 해야 할 일도 많아진다. 특히 새봄의 시작 3월에는 봄 농사철을 준비해야 하는 경칩이 있어 농사준비도 해야 하고 겨우내 하지 못했던 젖소들의 사양관리 점검 및 주변 환경을 돌아보고 새봄 맞이할 준비 시기이다. 이렇듯 새로운 마음가짐으로 경쟁력 있는 낙농가가 되기 위한 기본적인 사항부터 다시 점검하는 것이 필요하다. 가축의 생산성 향상은 가축개량을 통한 고능력우 확보로 그 기반을 닦고, 세균 및 체세포수 감소에 의한 우유 위생등급 향상, 조사료 다급 등을 통한 사양관리 기술 개발로 유지율 및 유단백을 향상한다. 또한 번식률 향상에 의한 산유량 증대와 부산물 수입 증대, 고능력 젖소의 이용연한 연장에 의한 가축감가상각비 절감에 대해 중점적으로 관심과 노력을 기울인다. 이를 위한 새봄맞이 젖소관리 방안에 대하여 기술해 보고자 한다.