• Korean Chemical Engineering Research
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• v.50 no.5
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• pp.879-884
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• 2012

In this present study, Aspergillus niger NRRL 567 was cultivated on an inert support material and the effects of various fermentation parameters including temperature, nutrient solution pH, inoculation level, and moisture content were observed and optimized by one-factor-at-a-time (OFAT) and response surface methodology (RSM), sequentially. It was found that the incubation temperature of $30^{\circ}C$ with 75% moisture content, nutrient solution pH of 7.1 and inoculation level of $4.0{\times}10^6$ spores/ml were the most favorable. Again, fermentation parameters were optimized using RSM. The determined optimum condition is $26.5^{\circ}C$, pH 9.9, 75.1%, and $6.0{\times}10^6$ spores/ml. Under this optimized condition, A. niger NRRL 567 produced 118.8 g citric acid/kg dry peat moss at 72 hr. Maximum citric acid production of optimized condition by RSM represented a 1.6-fold increase compared to that obtained from control experiment.

• Journal of Bio-Environment Control
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• v.17 no.2
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• pp.138-142
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• 2008

To develop suitable alternative substrates in hydroponics of sweet pepper, changes of water contents of substrates and electric conductivity (EC) of drainage nutrient solution, growth and fruit characteristics according to the kind of coir substrates were investigated compared with rockwool. In all coir substrates compared with rockwool during growing period, water contents were higher, EC of drainage nutrient solution except for fiber 50% were lower, and the coefficient of variation were a little, especially were so in fiber 30% of coir substrates. Plant growth in fiber 30% substrate was better than others but there was no significant difference. Photosynthesis rate and chlorophyll content were best in fiber 50% substrate. Fruit weight was no significant difference, but LID ratio and shape of the fruits were good in fiber 50% substrate and rockwool as which were close to regular square. Therefore, it was estimated that mixed coir substrates with fiber $30{\sim}50%$ are enough in possibility as alternative substrate.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.305-310
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• 2010

This study was carried out to investigate the effect of air humidity and water content of medium on the growth and physiological disorder of paprika in summer hydroponics. Treatments were composed of air humidity of control (over 90%) and dehumidification (low 90%) and water content of 80% and 50%. Time domain reflectometry (TDR) sensors and dehumidifier were used in a drip irrigation system and control system of air humidity, respectively. The early growth of paprika was not affected by air humidity but increased by high water content (80%) of medium. Mean fruit weight was reduced at high air humidity and low water content (50%) of medium, but the fruit number per plant and yield were increased at low air humidity. The incidence of brown fruit stem increased with increasing air humidity and water content of medium. Rate of blossom end rot increased in the low water content medium compared with the high water content medium. The nitrogen (N) was higher content in brown fruit stem than normal stem, but calcium (Ca) was lower.

• Journal of Bio-Environment Control
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• v.22 no.3
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• pp.248-255
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• 2013

This study examined an automated irrigation technique by a frequency domain reflectometry (FDR) sensor for scheduling irrigation for tomato (Solanum lycopersicum L. 'Starbuck F1') cultivation aimed at avoiding effluent from an open hydroponic system with coir substrate containing different ratios of chip-to-dust (v/v) content. Specifically, the objectives were to undertake preliminary measurements of irrigation volumes, leachate volume, volumetric water content and electrical conductivity (EC) in the substrate, plant growth, fruit yield, and water use efficiency resulting from variation in chip content as an initial experiment. Commercial coir substrates containing different percentages of chips and dust (0 and 100%, 30 and 70%, 50 and 50%, or 70 and 30%), two-story coir substrates with different percentages of chips in the lower layer and dust in the upper layer (15 and 85%, 25 and 75%, or 35 and 65%), or rockwool slabs were used. The results showed that a negligible or no leachate was found for all treatments when plants were grown under a technique for scheduling non-drainage irrigation using a frequency domain reflectometry (FDR) sensor. Daily irrigation volume was affected by chip content in both commercial and two-story slabs. The highest plant growth, marketable fruit weight, and water-use efficiency were observed in the plants grown in the commercial coir slab containing 0% chips and 100% dust, indicating that the FDR sensor-auto-mated irrigation may be more useful for tomato cultivation in coir substrate containing 0% chips and 100% dust using water efficiently and minimizing or avoiding leachate and thus increasing yield and reducing pollution. Detailed experiment is necessary to closely focus on determining appropriate irrigation volume at each of irrigation as well as duration of each individual irrigation cycle depending on different physical properties of substrates using an automated irrigation system operated by the FDR sensor.

• Journal of the Korean Wood Science and Technology
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• v.28 no.1
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• pp.55-64
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• 2000

Culture maturity assessment can be used to control fruiting body flush timing. Culture maturity of sawdust-based substrate was evaluated by using oak mushroom, (Lentinula edodes (Berk.) Pegler). The influence of substrate water potential (${\psi}$) on the growth and fruiting of three genotypes of L. edodes was also investigated. Glucosamine content revealed a peak at the fruiting body senescent stage. Glucosamine increased steadily to the sporophore senescent stage, and sharply declined at crop treatment. Lipid phosphate and ergosterol contents peaked at pinning and button break stages, respectively. Therefore lipid phosphate and ergosterol contents would be considered as the convenient measurement for judging culture maturity and fruiting potentials. The substrate pH values before inoculation and on the fruiting stage were varied from 6.3 to 4.0. This pH changes were detected as changes in color from bluish purple to yellow by direct bromphenol blue(BPB) spraying, and shown a good correlation with fruit body yield of the 1 st flush. Concerning water potential of the cultures, a slight reduction of water potential, -0.5MPa, stimulated mycelial and colony growths on liquid, agar and sawdust-based substrates. The water potential of well-colonized matured substrate was -0.7MPa and -4.0MPa, before and after the fruiting, respectively. Excellent water providing capacity (higher ${\psi}$) is expected to well-matured cultures with a high density of mycelial colonization. Also, the substrate water potential significantly affected by the interaction between genotypes and spawn run time.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1999.11a
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• pp.71-74
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• 1999

농업ㆍ원예분야에서 다공질 재료를 인공배지로 이용하기 위하여는 수분관리의 용이성, 우수한 양분의 유지, 통기성 및 보수성이 요구된다. 이들 성질을 만족시키는 재료로는 점토, 벤토나이트, 질석(vermicutite), 펄라이트(perlite) 및 락-울(rock-wool) 등이 이용된다. 인공배지 재료로 널리 사용되는 펄라이트와 락-울의 원료는 중국 및 호주 등지로부터 수입에 의존하고 있어 양액재배 농가에 커다란 부담이 되며 건습조절이 곤란하여 배지 내 수분함량의 조절이 용이하고 양액사용이 간편한 배지 및 폐기처리시 환경문제를 야기하지 않는 반영구적이며 자연친화형의 배지의 개발이 절실히 요구되고 있다. (중략)

• Journal of Bio-Environment Control
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• v.9 no.1
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• pp.20-26
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• 2000

This study aims at analyzing physical and chemical properties of perlite substrate. Particles of substrate were divided into five categories In size: gravel, very-coarse, coarse, medium and fine grade. Particles of very-coarse and greater grades in perlite substrate occupied 98.5％ of total particles. The air phase of total particles was distributed between 76.8％ and 87.7％ with especially showing that of very-coarse grade was lower than that of coarse or smaller one. However, the liquid and solid phases were vice versa. The cation exchange capacity (CEC) measured was highest in the fine grade. In drainage experiments, the water being drained from the substrate increased with the ratio of drain area, and the 65~70％ of total water reduced within five minutes after irrigation. The drained volume was proportional to the depth of the substrate, which contained about 2mL.cm$^2$：by depth (cm). Due to quick reduction of the water in the substrate, the pF value increased in four minutes after irrigation and showed the highest negative correlation ($R^2$＝0.997) with the moisture content of the substrate. The physical and chemical properties including drainage characteristics analyzed in this study can be, therefore, utilized to control the moisture content of perlite substrate, efficiently.

• The Korean Journal of Mycology
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• v.9 no.3
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• pp.123-128
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• 1981

Three edible species, e.g. Lyophyllum sp. and Armillaria mellea introduced from Japan in 1979 and Auriaularia auricula-judae from Taiwan in 1978 were selected for artifcial cultivation. The media, MSDS for Lyophyllum sp, MHS and PDS for A. auricula-judae, and MSDS and MHS for A. mellea were selected. The range of optimum pH of Lyophyllum sp. was 6.0 and pH 8.0 for A. auricula-judae and pH 6.0 to 7.0 for A. mellea. The range of optimum temperature for the mycelial growth of Lyophyllum sp. was 25 to $30^{\circ}C,\;30^{\circ}C$ for A. auricula-judae and $25^{\circ}C$ for A. mellea. When the moisture content 75 to 80% (w/w) in the substrates, the mycelial growth of Lyophyllum sp. and A. auricula-judae, was rapid. 65% (w/w) for A. mellea. As the spawning substrates, wheat for Lyophyllum sp. oak sawdust for A. auricula-judae and poplar sawdust for A. mellea were selected, respectively. In this experiment, yields of Lyophyllum sp. and A. auricula-judae were high on the poplar sawdust added 20% (w/w) of rice bran. Lyophyllum sp. and A. auricula-judae for sources of new edible mushrooms seems to be possible.

• Journal of Bio-Environment Control
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• v.18 no.3
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• pp.238-243
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• 2009

To investigate the characteristics of moisture content (MC), moisture distribution and starting point of drainage in a rockwool slab culture, time domain reflectometry (TDR) sensors were used in a drip irrigation system. MC values ($0{\sim}100%$) measured by TDR sensors in a slab were compared to those by loadcells. Seventy two seedlings of paprika (Capsicum annuum L.) were cultured for $5{\sim}6$ months in a green-house and the starting point of irrigation was determined by the average value of three TDR sensors which were inserted diagonally across the slabs under the plants. MCs as a standard for starting point of irrigation by TDR were determined with 40%, 50%, and 60%. Distribution of MCs in a slab measured with five TDR sensors equally spaced from two irrigation points were not much different when the MC in the slab increased from zero to saturation point. The saturated MCs in the slab were presented at $58{\sim}65%$ and the drain was started when the MC became around $50{\sim}55%$. At the saturated MC in the slab, TDR sensors presented 100% but the values from the loadcell showed 90% at the same time. However, measurement errors between two methods for MC remarkably decreased with a decrease in the MC in a slab. Especially when the MC was maintaining below 60%, the errors between TDR and loadcell methods for measuring MC in the rock-wool slab were less than 5%. There were no significant differences in number of fruits and fresh and dry weights of fruits when they were cultured under the different MC conditions with three irrigation regimes (40%, 50%, and 60%). These results indicated that the MC control by TDR sensors in a rock-wool based paprika culture can be suggested as a method to determine the starting point of irrigation for a soilless culture system.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1999.11a
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• pp.217-220
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• 1999

수경재배중 고형배지 재배에서 주로 이용되어 왔던 배지로는 펄라이트, 암면, 질석, 폴리우레탄(PUR) 및 코코피트 등이 있다. 이중 펄라이트와 암면의 재배면적은 1998년에 전체 수경재배면적 553.4ha에서 각각 275.2ha와 163.1ha의 높은 점유율을 나타내고 있다. 이와 최(1998)은 수경재배 배지로 펄라이트와 암면이 가장 가능성이 높다라고 하였으며, Wilson(1986)도 토마토와 오이 수경재배에 있어서 가장 이상적인 배지는 펄라이트라고 하였 다. (중략)