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http://dx.doi.org/10.9713/kcer.2012.50.5.879

Response Surface Optimization of Fermentation Parameters for Citric Acid Production in Solid Substrate Fermentation  

Kim, Jin-Woo (Department of Biosystems Engineering, McGill University)
Publication Information
Korean Chemical Engineering Research / v.50, no.5, 2012 , pp. 879-884 More about this Journal
Abstract
In this present study, Aspergillus niger NRRL 567 was cultivated on an inert support material and the effects of various fermentation parameters including temperature, nutrient solution pH, inoculation level, and moisture content were observed and optimized by one-factor-at-a-time (OFAT) and response surface methodology (RSM), sequentially. It was found that the incubation temperature of $30^{\circ}C$ with 75% moisture content, nutrient solution pH of 7.1 and inoculation level of $4.0{\times}10^6$ spores/ml were the most favorable. Again, fermentation parameters were optimized using RSM. The determined optimum condition is $26.5^{\circ}C$, pH 9.9, 75.1%, and $6.0{\times}10^6$ spores/ml. Under this optimized condition, A. niger NRRL 567 produced 118.8 g citric acid/kg dry peat moss at 72 hr. Maximum citric acid production of optimized condition by RSM represented a 1.6-fold increase compared to that obtained from control experiment.
Keywords
Response Surface Optimization; Critic Acid; Aspergillus niger; Solid Substrate Fermentation;
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