• 약학회지
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• 제57권3호
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• pp.167-172
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• 2013

Insampaedoksan (IS) is the decoction of medicinal herbs, which was commonly used for anti-inflammatory and anti-pyretic in the Korean traditional medicine (KTM). Several studies on improving efficiency or searching new efficiency by fermenting traditional herbal medicines are recently in progress. The bioconversion has been conducted on IS using various bacteria. Liquiritin, ferulic acid, hesperidin, liquiritigenin, and glycyrrhizin in IS before and after fermented IS were simultaneously analyzed. These compounds were qualitatively analyzed and quantitatively analyzed using the HPLC-DAD system. The identifications of liquiritin, ferulic acid, hesperidin, liquiritigenin and glycyrrhizin were achieved by comparing the HPLC retention time ($R_t$) and the UV absorption of five pure compounds in the IS. As a result, the increased constituents were identified to be liquiritin, liquiritigenin and glycyrrhizin, while the decreased constituent was ferulic acid and the constituent of hesperidin was similar to before and after fermentation. Insampeadock-san fermented by Lactobacillus plantarum KFRI 144 exhibited the most remarkable changes in all of fermentation.

• 사상체질의학회지
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• 제22권2호
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• pp.101-107
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• 2010

1. Objectives: The aim of this study is to investigate the acute toxicity and safety of Taeeumjowi-tang. 2. Methods: We investigated the acute toxicity for water-extracted Taeeumjowi-tang. 25 male and 25 female mice were observed for 14 days after one day oral administration of Taeeumjowi-tang at the respective doses of 0(control group), 2560, 3200, 4000 and 5000 mg/kg. 3. Results: We observed survival rates, general toxicity, change of body weight and autopsy. 4. Conclusions: The data confirmed that Taeeumjowi-tang is free from the toxicity and safety problems in oral route respectively. Compared with the control group, we could not find any toxic alteration in all treated groups(2560, 3200, 4000 and 5000 mg/kg). In conclusion, LD50 of Taeeumjowi-tang was over 5000 mg/kg and it is very safe to mice.

• 한방비만학회지
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• 제9권2호
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• pp.11-19
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• 2009

Objectives In this study, we investigated the acute toxicity and safety about fermented Bangpungtongsung-san(Fangfengtongsheng-san) extract. Methods To evaluate the acute toxity and safety, fermented Bangpungtongsung-san (Fangfengtongsheng-san) at the respective doses of 0(control group), 1250, 2500 and 5000 mg/kg were orally treated to 20 male and 20 female mice. After single administration, we observed survival rates, general toxicity, changes of body weight and autopsy. Results Compared with the control group, we could not find any toxic alteration in all treated groups (1250, 2500 and 5000 mg/kg). Conclusions $LD_{50}$ of fermented Bangpungtongsung-san(Fangfengtongsheng-san) extract might be over 5000 mg/kg and it is very safe to ICR mice.

• 한방비만학회지
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• 제9권1호
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• pp.71-78
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• 2009

Objectives Bangpungtongsung-san(Fangfengtongsheng-san) has been traditionally prescribed a a restorative medicine. Methods In this study, we investigated the acute toxicity of water-extracted Bangpungtongsung-san(Fangfengtongsheng-san). 20 male and 20 female mice were orally treated of Bangpungtongsung-san(Fangfengtongsheng-san) at the respective doses of 0 (control group), 1250, 2500 and 5000mg/kg for 14days. Results We observed survival rates, general toxicity, change of body weight and examined microscopic changes of some organs. Conclusions Compared with the control group, we could no find any toxic alteration in all treated groups (1250, 2500 and 500mg/kg). $LD_{50}$ of Bangpungtongsung-san(Fangfengtongsheng-san) might be over 5000mg/kg and it was very safe to ICR mice.

• 농업과학연구
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• 제42권3호
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• pp.285-291
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• 2015

Many Koreans have the question about the price of food: whether the price of Korean food is reasonable or not? Even though some previous studies tried to discuss and analyze the price system of Korean food, few papers clearly present the implication about production cost or marketing margin of processing food. The purpose of this paper is to measure and analyze the production cost and marketing margin of tofu and kimchi, which are one of the main food in Korea, through the raw-data of FIS, KAMIS, and other data from Korean business area. The results of studies present a few findings as follows: First, the proportion of labor cost in the production cost is very important factor and need to be decreased for the lower consumer price. Especially, the lower proportion of labor cost in kimchi industry should be important issue, even though the reduction of proportion of labor cost in kimchi industry is not easy in the real world. Second, each marketing channel of processed food shows different marketing margin. Therefore, Korean government need to increase the level of competition of marketing channels, which makes each business is forced to decrease the marketing margin to survive the market competition in Korea.

• 한국전자파학회논문지
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• 제7권4호
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• pp.288-299
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• 1996

무선 중계시스템을 통한 전파 전송방식중 동일 채널 중계방식은 현재 보면적으로 사용중인 인터리브드방식에 비하여 특히 주파수 이용 효율변에서 커다란 장점을 자기고 있으므로, 한정된 주파수 자원의 효율적 운용을 위하여 이바한 동일 채널 중계방식의 개발 및 적용에 대한 연구는 매우 중요한 의미를 지닌다. 본 논문에서는 lOGHz 이하 대역에서 동일 채널 무선 중계방식을 사용하는 디지탈 시스템의 성능 및 페이드마진 계산 방안을 제시하였으며 시뮬레이션을 통해 실제 상황에의 적용방안을 다루었다.

• 대한한의학방제학회지
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• 제21권1호
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• pp.111-118
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• 2013

Objectives : Traditional medicine Oryeong-san (ORS) has been prescribed for a long time to treat light fever, thirst, dysuria, and accompanying edema. However, the acute toxicity and safety were not reported. In this study, we evaluated the potent acute toxicity and safety of ORS. Methods : ICR mice were used to evaluate acute toxicity and safety by oral administration of 0, 500, 1,000, 2,000mg/kg of ORS. Mortality, body weight, and clinical symptoms were observed, and organ weight and blood biochemical parameters were analyzed after necropsy. Results : We found no mortality and no toxic or abnormal clinical symptoms by administration of ORS. Comparing with control group, no significant alterations in organ weight and blood biochemical parameters were observed. Conclusions : ORS recognized as safe and non-toxic medicinal material, and median lethal dose considered to be over 2,000 mg/kg in both male and female ICR mice.

• 대한한의학회지
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• 제29권3호
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• pp.1-10
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• 2008

Objectives: This study was to assess the toxicity of Sipjeondaebo-tang by bacterial reverse mutation test. Methods: In this study, to evaluate the bacterial reverse mutation of Sipjeondaebo-tang water-extract, the in vitro Ames test using Salmonella typhimurium (TA98, TA100, TA1,535, TA1,537) and Escherichia coli(WP2uvrA) were performed with Sipjeondaebo-tang water extract at the concentrations 0, 312, 625, 1250, 2500 and 5000 ${\mu}g/plate$. Results: Sipjeondaebo-tang water extract was negative in Ames test with both Salmonella typhimurium or Escherichia coli with and without rat liver microsomal enzyme (S9- fraction and S+ fraction). Conclusions: According to these results, we concluded that a Sipjeondaebo-tang water extract did not cause bacterial reverse mutation.

• Toxicological Research
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• 제15권1호
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• pp.103-107
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• 1999

In order to evaluate acute toxicity of Coptidis rhizoma, 6 week- and 13 week-old male ICR mice received Coptidis rhizoma extract (600~4,800 mg/kg body weight) orally, and toxicological responses were observed for consecutive 7 days. In the mice received relatively high concentration of Coptidis rhizoma($\geq$1,200mg/kg), death occurred within 3 hrs after oral administration, and its ratio in 13 week-old mice was conspicuously higher than that in 6 week-old mice. $LD_{50}$ of Coptidis rhizoma were estimated to bi 2,575 mg/kg and 1,490 mg/kg body weight in 6 week and 13 week-old mice, respectively. Coptidis rhizoma-treated animals manifested a variety of abnormal clinical findings such as ptosis, crouching, lethargy, convulsion, bizarre behavior and truning sideway. These abnormalities also ranked highly in the 13 week-old mice compared to those in the 6 week-old mice. In addition to abnormal behaviors, Coptidis rhizoma($\geq$1,200 mg/Kg) significantly elevated the urinary contents of bilirubin, urobilirubin, protein and glucose, and values in 13 week-old mice was higher than those in 6 week-old animals. No toxicological response was observed at concentration less than 600 mg/kg. Our results clearly demonstrate that susceptibility of mice to Coptidis rhizoma may be related with age, indicating that younger age mice is more resistant to the Coptidis rhizoma than the older, and toxicological mechanism of Coptidis rhizoma may be closely associated with its pharmacological mechanism.

• 생약학회지
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• 제40권3호
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• pp.224-228
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• 2009

For the quality control of traditional herbal medicine, Galgeun tang, simultaneous determination of glycyrrhizin, paeoniflorin, puerarin, 6-gingerol was established by using a high performance liquid chromatographic (HPLC) method with diode array detector. To separate five four constituents, DIONEX $C_{18}$ column ($5{\mu}m$, $120{\AA}$, $4.6\;mm{\times}150\;mm$) was used with gradient elution system of water and methanol. Validation of the chromatography method was evaluated by linearity, recovery, and precision test. Calibration curve of standard components showed excellent linearity ($R^2$>0.9906). Limits of detection (LOD) and limits of quantification (LOQ) varied from 0.15 to $0.52{\mu}g$/ml and 0.27 to $0.80{\mu}mg$/ml, respectively. The relative standard deviations (RSDs) of data of the intra-day and inter-day experiments were less than 2.88% and 1.21%, respectively. The results of recovery test were ranged from 96.71 to 106.29% with RSD values 0.01-0.80%.