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Prey Consumption and Suppression of Vegetable Aphids by Chrysopa pallens (Neuroptera: Chrysopidae) as a Predator (채소류 진딧물에 대한 칠성풀잠자리붙이의 포식량 및 밀도억제 효과)

  • 이건휘;이승찬;최남영;김두호
    • Korean journal of applied entomology
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    • v.39 no.4
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    • pp.251-258
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    • 2000
  • These studies were conducted to investigate the prey consumption and suppression of cotton aphid and green peach aphid by Chrysopa pallens Ramber as a predator. The 3$^{rd}$ instar of C. pallets fed on 29.8, 77.9, 133.6, and 155.7 individuals of apterous Aphis goussypii Glover a day at 17,22, 27, and 32$^{\circ}C$, respectively. A preovipositing female fed on 73.1 individuals, ovipositing female on 86.6 individuals, and adult male on 69.7 individuals of apterous Myzus persicae (Sulzer) a day at the 27$^{\circ}C$. The functional response curve of the larvae and adults of C. pallens to the densities of A. gossypii indicated Helling’s Type II: the consumption of prey by the C. pallens increased with the prey density but the consumption rate decreased. The attack rate of 3rd instar of C. pallens was the highest among the 2nd instar, 3$^{rd}$ instar, adult male and adult female, and handling time was the shortest. The critical ratio of the predator vs. the prey to effectively suppress the population of A. gossypii by releasing C. pallets eggs was 1 : 4 on red pepper and egg plant, and 1 : 3 on cucumber. Release of second larval stave of C. pallens at the ratio of 1 : 30 of the predator vs. the prey controlled satisfactorily A. gossypii on red pepper, and 1 : 20 on cucumber and tomato. The three-times introduction of the eggs of C. pallens was as effective as four applications of insecticides from mid-June to late September.r.

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Pathogenicity of Entomopathogenic Nematode, Steinernema carpocapsae Pocheon Strain Against Anomia mesogona and Anomis commoda(Lepidoptera: Noctuidae) (무궁화잎밤나방(Anomis mesogona)과 큰붉은잎밤나방(Anomis commoda)에 대한 Steinernema carpocapsae 포천 계통의 병원성)

  • Kim Hyeong-Hwan;Park Hyung-Soon;Cho Yoon-Jin;Lee Dong-Woon;Choo Ho-Yul;Goo Kwan-Hyo
    • Asian Journal of Turfgrass Science
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    • v.19 no.1
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    • pp.17-25
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    • 2005
  • Pathogenicity of entomopathogenic nematde, Steinernema carpocapsae Pocheon strain (ScP) was evaluated against different larval stages(2nd, 3-4th and 5th) of Anomis commode and Anomis mesogona(Lepidoptera: Noctuidae) in petri dish and pot. The $LC_{50}$ values were increased in proportion to larval stage of A. commoda and A. mesogona. $LC_{50}$ value of ScP against 2nd instar of A. commoda and A. mesogona was 9.7 and 4.5, respectively. The 2nd instar of both species was also susceptible to ScP in pot test. That is, mortality of 2nd instar of A. commode and A. mesogona was higher representing $72.5\%\;and\;87.5\%$ 5 days later after treatment, respectively, when ScP was applied at the ,ate of 90,000 infective juveniles(Ijs) per $pot(=1\times10^9\;Ijs/ha)$. However, susceptibility was decreased from 3rd instar. Mortality of 5th instar of A. commoda and A. mesogona was $5.0\%\;and\;10.0\%$, respectively, at the rate of 90,000 Ijs/pot. When ScP was applied into pot including mixed larval stages from 2nd to 5th instar(10 larvae far 2nd instar, 10 larvae for 3rd - 4th instars, and 10 larvae for 5th instar) at the rate of 90,000 Ijs/pot, mortality of A. commoda and A. mesogona was $69.2\%\;and\;50.0\%$, respectively.

Pathogenicity of Korean Entomopathogenic Nematodes to Exomala orientalis(Coleoptera : Scarabaeidae) (등얼룩풍뎅이(Exomala orientalis)에 대한 한국산 곤충병원성 선충의 병원성)

  • Lee Dong-Woon;Kim Hyeons-Hwan;Lee Sang-Myeong;Choo Ho-Yul;Choi Woo-Goun;Kweon Tae-Woong
    • Asian Journal of Turfgrass Science
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    • v.19 no.1
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    • pp.39-46
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    • 2005
  • The 21 strains of Korean entomopathogenic nematodes, {Heterorhabditis bacteriophora Hamyang strain(HbH), Heterorhabditis sp. 202, 205, 217, Heterorhabdiris sp. KCTC 0991BP strain, Steinernema carpocapsae Pocheon(ScP), S. longicaudum Gonaju, S. longicaudum Nonsan, Steinernema sp. 7,24, 52, 55, 60, 64, 206, 207, 209, 210, 219, and 227 strain} were evaluated for the control of a turfgrass insect pest, Exomala orientalis. Heterorhabditis spp. showed higher pathogenicity than Steinernema spp. against 3rd instar larvae of E. orientalis with $55\%$ mortality by Heterorhabditis sp. 202 strain and $50\%$ by HbH and Heterorhabditis sp.205 strain at the rate of 200 infective juveniles per larva 14 days later after treatment. The number of infective juveniles of Korean entomopathogenic nematodes in 3rd instar larvae of E. orientalis was higher in Heterorhabditis spp. than in Steinernema spp.. In general, numbers of produced infective juveniles of three species were much higher, i.e., Heterorhabditis sp.202 strain produced 273,064 infective juveniles, S. carpocapsae Pocheon strain 273,043, and Heterorhabditis sp. 217 strain 248,887, respectively.

Possibility of Control of Turfgrass Insect Pest, Popillia quadriguttata(Coleoptera : Rutelidae) Using Pheromone Trap in Golf Course (페로몬 트랩을 이용한 골프장 잔디해충 녹색콩풍뎅이의 방제가능성)

  • Kim, Jae-Ho;Lee, Jae-Pil;Ham, Suon-Kyu;Kim, Doo-Hwan;Yeom, Ju-Rip;Lee, Dong-Woon
    • Asian Journal of Turfgrass Science
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    • v.23 no.1
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    • pp.45-60
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    • 2009
  • Occurrence ecology and possibility of mass trapping of Popillia quadriguttata, one of the most serious turfgrass insect pest in domestic golf courses was investigated using pheromone traps in Gimpo and Jinhae. Peak period of adult occurrence of P. quadriguttata was late June and early July in Gimpo Seaside Country Club from 2006 to 2008. It was early and middle July in Youngwon Country Club in 2006. The first catched day of P. quadriguttata adult was 20 June, while the last day was 10 September in Gimpo Seaside Country Club, 2006. Mean catched number of P. quadriguttata adult in Japanese beetle pheromone trap per day in peak period of occurrence were 18.2, 25.7, 29.0, and 15.7 at 2005, 2006, 2007 and 2008 respectively. Mean catched number of P. quadriguttata adult in Japanese beetle pheromone trap were more in fairways or roughs than tees or greens. Possibility of control of scarab beetle using pheromone trap with pesticide treatment was investigated against oriental beetle, Blitopertha orientalis in Dongrae Benest Golf Club. Mean number of dead oriental beetle adult were more higher(2.9 folds) in fenitrothion EC treatment than fenitrothion EC treatment after set oriental beetle pheromone trap.

Survey on Nematodes in Cymbidium and Chemical Control of Ditylenchus sp. (심비디움에 발생하는 선충 조사 및 줄기선충류(Ditylenchus sp.)에 대한 약제방제 효과)

  • Cho, Myoung-Rae;Kang, Taek-Joon;Kim, Hyung-Hwan;Ahn, Seung-Joon;Jeon, Sung-Uk;Chun, Jae-Yong;Kim, Young-Ho
    • Korean journal of applied entomology
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    • v.51 no.2
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    • pp.153-156
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    • 2012
  • Surveys were conducted on the occurrence of nematodes in the root systems of 1-3-year old Cymbidium hybida Swartz cultivated for export in Korea. The most frequently detected plant-parasitic nematode was Ditylenchus sp. with 4.7, 43.7, and 49.7/200 cc growth medium in 1, 2, and 3 year-old cymbidiums, respectively. And the densities of non-parasitic nematodes, dorylaimids, were 35.3, 70.5, and 155.8/200 cc growth medium in 1, 2, and 3-year-old cymbidiums, respectively. Three-year-old cymbidiums collected from Siheung, Ansan, and Gimpo had low densities of Aphelenchus sp. and Aphelenchoides sp. with under 40 individuals/pot, and the dorylaimid densities were 56-824/pot. To evaluate the effects of nematicides on Ditylenchus sp. in cymbidium, Emamectin benzoate EC, Fosthiazate SL, and Cadusafos CS were tested at two farms in Ansan and Gimpo. Emamectin benzoate EC showed control effects of 75.7 and 89.5%, whereas Fosthiazate SL and Cadusafos CS showed 27.2 and 65.3% and 30.1 and 90.5% control effects in the tests.

The Efficacy of Surgical Treatment with Flexible Alligator Forceps in Dogs with Heartworm Infection (심장사상충 감염견에서 Flexible Alligator Forceps을 이용한 외과적 치료)

  • Yoon Hun-young;Jeong Soon-wuk;Kim Jun-young;Han Hyun-jung;Jang Ha-young;Lee Bora;Namkung Hyo-sun
    • Journal of Veterinary Clinics
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    • v.22 no.4
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    • pp.309-313
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    • 2005
  • This study was performed to assess the efficacy of surgical treatment with flexible alligator forceps (FAF) on heartworm infected dogs. Twenty dogs (10 males and 10 females) with heartworm infection, age $5.3\pm3.0\;(mean{\pm}S.D.)$ years and weight $9.3\pm6.5(mean{\pm}S.D.)kg$ were treated with FAF. Adult heartworms were removed by fluoroguided technique with FAF under inhalation anesthesia with isoflurane. For evaluation of the efficacy, removal rate and surgical time were measured, and removal sites were found. Antigen ELISA kit test and autopsy were performed to examine remained adult heartworms. Removal rate of total heartworms was $91.4\%$ including 15 complete retrieval cases and surgical time was $30.0\pm7.6(mean{\pm}S.E.)$ minutes. Heartworms were removed from the right ventricle, main pulmonary artery, and right and left pulmonary artery. Surgical treatment with FAF is an effective method with high removal rate, short surgical time, and various removal sites and it could be considered for the removal of adult heartworms in a dog.

The Study of the Taste and the Intake-Frequency for Kwamaegi - Centering around Kyungbuk Regions- (꽁치과메기에 대한 선호도 및 섭취빈도에 관한 연구)

  • 조영대;김정애;오승희
    • The Korean Journal of Food And Nutrition
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    • v.13 no.6
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    • pp.585-594
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    • 2000
  • We made a survey of the pattern of dietary life, the taste and the intake-frequency for Kwamaegi in Kyungbuk province. And the results were summarized as follows : 1. Flavor(65.2%), nutritive value(14.6%), cleanliness(14.3%), price(4.3%) and calorie(0.7%) were considered to select food in that order. 2. The younger, the more they preferred foreign food to Korean food and the older, the more they preferred Korea food to foreign food. 3. Most of people(91.4%) answered positively to Kwamaegi and had a high preference of Kwamaegi. On most occasions, they were inclined to eat Kwamaegi as the side-dish(71.1%) served with drink. 4. Most of men(61.1%) have eaten 3∼10 Kwamaegis at a table, but most of women(53.7%) have eaten 1∼2 Kwamaegis at a table.

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Production of Supermale(YY) and Superfemale(${\Delta}YY$) Nile Tilapia (Oreochromis niloticus) by Sex Reversal and Chromosome Manipulation II. Progeny Tests with Supermale and Superfemale Nile Tilapia (성전환 및 염색체 공학 기법을 이용한 초수컷(YY) 및 초암컷(${\Delta}YY$) 나일틸라피아(Oreochromis niloticus) 생산 II. 생산된 초수컷 및 초암컷 나일틸라피아의 자손검정)

  • Kim Dong Soo;Noh Choong Hwan;Choi Yoon-Hee;Nam Yoon Kwon
    • Journal of Aquaculture
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    • v.9 no.1
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    • pp.101-106
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    • 1996
  • Gynogenetic males induced from sex reversed female (${\Delta}XY$) were crossed with normal female (XX) for analysing their genotypes. The fish tested produced a high percentage of male progenies (93.3 to $100\%$) and were considered as supermales (YY). Superfemales (${\Delta}YY$) were also produced by combination of sex reversal and chromosome manipulation techniques. Superfemale fish can be produced approximetly $90\%$ of male when the fish were crossed with normal male. Chi-square values against an expected 1 : 1 (male : female) ratio were highly significant for both YY males${\times}$ normal females (P<0.01 or P<0.001) and ${\Delta}YY$ females${\times}$normal males (P<0.005 or P<0.001). All male progenies were produced consistently when crossed supermales (YY) with superfemales (${\Delta}YY$).

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Biological Control of Arge Captiva, Arge Pagana Papana, and Arge Similis with Entomopathogenic Nematodes (곤충병원성 선충을 이용한 홍가슴루리등에잎벌(Arge captiva), 장미등에잎벌(Arge pagana papana) 및 극동등에잎벌(Arge similis)의 생물적 방제)

  • Yang, Jae Yun;Kim, Hyeong Hwan;Lee, DongWoon;Lee, Sang Myeong;Shin, Hyeon Chul;Choo, Ho Yul
    • Journal of Korean Society of Forest Science
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    • v.96 no.1
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    • pp.1-6
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    • 2007
  • Entomopathogenic nematodes (Heterorhabditis sp. Gyeongsan strain, Steinernema carpocapsae GSN1 strain, S. feltiae Monteri strain, S. glaseri Dongrae strain, S. longicaudum Nonsan strain and S. monticolum Jiri strain) were evaluated for the environmentally sound control of sawfly, Arge captiva, A. pagana pagana and A. similis in the laboratory and pot. The corrected mortality of 3rd instar of Arge captiva larva was 100% at 5 days after treatment with S. carpocapsae GSN1 strain and S. feltiae Monteri strain in Petri dish. The mean numbers of established infective juveniles (Ijs) of S. glaseri Dongrae and S. carpocapsae GSN1 strain in a Arge captiva larva were 10.2 and 4.2 Ijs/larva, respectively. Pathogenicity of S. carpocapsae GSN1 strain was different larval stage, i.e., $LC_{50}$ value of S. carpocapsae GSN1 strain against 2nd, 3rd and 4th instar of A. pagana pagana was 11.5, 9.3, and 8.4 Ijs, respectively. Mortality of Arge captiva, A. pagana pagana and A. similis were 72.5, 85.0 and 85.0% by S. carpocapsae GSN1 strain at the $2{\times}10^9Ijs/ha$, respectively, in the pot.

Effect of Oriental Anti-Stress Agent(Bohyulanshintang) on the Salivary Gland of Rats under Restraint Stress (보혈안신탕(補血安神湯)이 스트레스에 의한 백서 타액선 조직 변화에 미치는 영향에 관한 연구)

  • Ryu, Ho-Kyung;Chun, Yang-Hyun;Lee, Jin-Yong;Hong, Jung-Pyo
    • Journal of Oral Medicine and Pain
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    • v.24 no.3
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    • pp.245-257
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    • 1999
  • 일상 생활에서 우리는 스트레스에 항상 노출되어 있으며, 스트레스는 생체의 신경계, 내분비계 및 면역계의 변화를 수반한 항상성의 파괴로 수많은 정신적, 육체적 질병을 야기시킨다. 특히 구강안면영역에서도 다양한 구강점막질환과 구강건조증 등을 발생시킨다. 스트레스를 제거하는 방법으로는 약물요법 및 상담, 명상요법, 종교요법 등 다양한 방법이 제시되고 있는데, 다소의 부작용이 나타나거나 꾸준히 시행하기가 쉽지 않으며 스트레스의 원인을 근본적으로 제거하기가 현실적으로 용이하지 않은 경우가 많아 스트레스에 대한 해결책에 대하여 많은 관심이 집중되고 있다. 이에 본인은 스트레스가 가해졌을 때 백서 악하선에서 관철되며 apoptosis에 대하여 세포보호작용을 하는 clusterin(SGP-2)을 이용하여 구속스트레스를 가하기에 앞서 오랫동안 경험적으로 사용되어 왔고 부작용이 적은 전통약물인 보혈안신탕을 투여하고 스트레스에 의한 타액선의 조직변화를 관찰하여 그 효과를 확인해 보고자하였다. Sprague-Dawley계 응성 백서(200-230g/bw) 33마리를 정상 대조군(3마리), 구속스트레스군(15마리) 및 보혈안신탕 투여 후 구속스트레스군(15마리)으로 나누고 이틀을 각각 구속장치에 구속한 후 0, 1, 3, 5, 7일에 회생시켜 악하선을 적출하였으며, 면역조직화학법 및 Northern Blot을 이용하여 clusterin의 변화를 관찰하였다. 그 결과는 다음과 같다. 1. 구속스트레스군의 악하선 조직에서 clusterin 단백질과 mRNA는 실험 즉일군에서만 미약하게 관찰되었으며 실험 3일과 5일 후에 핵붕괴 및 핵농축 등의 핵변화를 동반한 apoptosis가 관찰 되었다. 2. 보혈안신탕 투여 후 구속스트레스군의 악하선 조직에서 실험 5일군까지 clusterin 이 증가한 후 실험 7일군에서는 감소하였다. 3. 보혈안신탕 투여 후 구속스트레스군의 악하선 조직에서는 apoptosis가 관찰되지 않았다. 4. 보혈안신탕 투여 후 구속스트레스군의 악하선 조직에서 clusterin mRNA가 실험 전군에 걸쳐 미약하게 관찰되었다. 이상의 결과로 타액선 조직은 스트레스 단백질인 clusterin을 생산하여 세포를 보호함으로써 스트레스 상황에 적응하지만, 생리적 적용한계를 넘는 스트레스에 노출될 때는 apoptosis됨이 확인되었다. 그리고 보혈안신탕은 스트레스 상황에서 세포의 생리적 적응력을 높여 세포의 apoptosis를 억제하는 효과를 나타냄이 확인되었다. 따라서 본 연구결과는 구강건조증등의 스트레스성 타액선 질환의 병리기전을 규명하는데 도움이되리라 생각되며, 향후 항스트레스 효과를 가진 보혈안신탕등의 한약재를 임상에 적용함으로써 스트레스로 인한 신체의 병리적 변화를 다소나마 차단할 수 있을 것으로 사료된다.

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