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Polishing characteristics of polyetherketoneketone on Candida albicans adhesion (Polyetherketoneketone의 연마 특성이 Candida albicans의 부착에 미치는 영향)

  • Kim, Hyunyoung;Lee, Jonghyuk;Lee, Sung-Hoon;Baek, Dongheon
    • The Journal of Korean Academy of Prosthodontics
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    • v.58 no.3
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    • pp.207-216
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    • 2020
  • Purpose: To compare the polishing characteristics and their influence on Candida albicans adhesion to the recently introduced polyetherketoneketone (PEKK) and the conventional polymethylmethacrylate (PMMA) denture resin material. Materials and methods: Specimens from PEKK (Group E) and PMMA (Group M) were made in dimensions of 8 mm in diameter and 2 mm in thickness. The specimens were further divided into sub-groups according to the extent of polishing (ER, MR: rough; EP, MP: polished, N = 12 each). The specimens were polished using polishing machine and SiC foil. ER and MR group specimens were polished with 600 grit SiC foil only. EP and MP groups were further polished with 800, 1,000, 1,200 grit SiC foils sequentially. To measure the surface roughness values (Sa) of specimens, atomic force microscope (AFM) was used and scanning electron microscope (SEM) observation under 1,000, and 20,000 magnifications was performed to investigate surface topography. The polished specimens were soaked in C. albicans suspension for 2 hours with shaking to promote adhesion. The attached C. albicans were detached from the surface with 10 times of pipetting. The suspension of detached C. albicans was performed by serial dilution to 103 times, and the diluted suspensions were inoculated on Sabouraud dextrose agar plates using spread plate method. After incubating the plate for 48 hours, colony forming unit (CFU)/plate of C. albicans was counted. Statistical analysis was performed using one-way ANOVA and Tukey HSD test to confirm significant difference between the groups (α=.05). Results: Average Sa value was significantly higher in MR group compared to other groups (P<.05), meaning that additional polishing steps reduced surface roughness effectively only in the PMMA specimens. There was no significant difference in Sa values between MP and EP groups. In SEM images, PEKK specimens showed numerous spikes of abraded material protruding from the surface and this phenomenon was more significant in EP group. The mean CFU/plate value was the highest in EP group and this was significant when it was compared to MP group (P<.05) which was the lowest. Conclusion: Polishing PEKK using serial SiC abrasive foil may result in higher adhesion of C. albicans. In clinic, this should be considered carefully.

Effects of NaCl Concentrations on Production and Yields of Fruiting Body of Oyster Mushrooms, Pleurotus spp. (NaCl의 농도가 느타리버섯 자실체 발생 및 수량에 미치는 영향)

  • Jhune, Chang-Sung;Sul, Hwa-Jin;Kong, Won-Sik;Yoo, Young-Bok;Cheong, Jong-Chun;Chun, Se-Chul
    • The Korean Journal of Mycology
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    • v.34 no.1
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    • pp.39-53
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    • 2006
  • This studies investigated the effect of concentrations of sodium chloride (NaCl) on occurrence and growth of fruitbody in oyster mushrooms, Pleurotus spp. Our experiments divided into two parts. When the water contents in substrate were added with sodium chloride solution in cotton waste box cultivation as a first experiment, the growth of mushroom was damaged as the concentration was increased, even though there was a little difference according to the strains. The yield in 1.0% NaCl solution was decreased to 72% compared to non-treated plot while that in 3.0% solution was only 2% of the non-treated plot. Morphological characteristics of mushrooms cultivated in substrate with the different concentration of the solution showed different results. For example, the size and thickness of pilei were not influenced by NaCl concentration, but the length of stipes and individual weight were much influenced. In plastic box cultivation filled with cotton waste, watering treatment with the different concentrations of sodium chloride solution, the second experiment, did not show any difference according to the concentration until 1.0% solution but there was a little difference according to the strains. The productivity of fruitbody started to decrease at 2.0% of the solution and the yield and quality of mushroom in 3.0% solution treatment were generally low. After the second flush, days for mushroom sprouting were generally prolonged in proportion to the solution concentration. Taken altogether, the second experiment did not show a clear effect as the case of the first experiment.

Magnetoresistance Effects of Magnetic Tunnel Junctions with Amorphous CoFeSiB Single and Synthetic Antiferromagnet Free Layers (비정질 CoFeSiB 단일 및 합성형 반강자성 자유층을 갖는 자기터널접합의 자기저항 효과)

  • Hwang, J.Y.;Kim, S.S.;Rhee, J.R.
    • Journal of the Korean Magnetics Society
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    • v.15 no.6
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    • pp.315-319
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    • 2005
  • To obtain low switching field ($H_{SW}$) we introduced amorphous ferromagnetic $Co_{70.5}Fe_{4,5}Si_{15}B_{10}$ single and synthetic antiferromagnet (SAF) free layers in magnetic tunnel junctions (MTJs). The switching characteristics for MTJs with structures $Si/SiO_2/Ta$ 45/Ru 9.5/IrMn 10/CoFe 7/AlOx/CoFeSiB 7 or CoFeSiB (t)/Ru 1.0/CoFeSiB (7-t)/Ru 60 (in nm) were investigated and compared to MTJs with $Co_{75}Fe_{25}$ and $Ni_{80}Fe_{20}$ free layers. CoFeSiB showed a lower saturation magnetization of $560 emu/cm^3$ and a higher anisotropy constant of $2800\;erg/cm^3$ than CoFe and NiFe, respectively. An exchange coupling energy ($J_{ex}$) of $-0.003erg/cm^2$ was observed by inserting a 1.0 nm Ru layer in between CoFeSiB layers. In the CoFeSiB single and SAF free layer MTJs, it was frond that the size dependence of the $H_{SW}$ originated from the lower $J_{ex}$ experimentally and by micromagnetic simulation based on the Landau-Lisfschitz-Gilbert equation. The CoFeSiB SAF structures showed lower $H_{SW}$ than that of NiFe, CoFe and CoFeSiB single structures. The CoFeSiB SAF structures were proved to be beneficial far the switching characteristics such as reducing the coercivity and increasing the sensitivity in micrometer to submicrometer-sized elements.

Beneficial Effect of an Agar Mask against Skin Damage Induced by UV Exposure in SKH-1 Hairless Mice (UV조사에 의해 유발된 SKH-1 hairless 마우스의 피부노화에 미치는 한천마스크의 개선효과)

  • Song, Bo Ram;Kim, Ji Eun;Yun, Woo Bin;Lee, Mi Rim;Choi, Jun Young;Park, Jin Ju;Kim, Dong Seob;Lee, Chung Yeoul;Lee, Hee Seob;Lim, Yong;Jung, Min Wook;Kim, Bae Hwan;Hwang, Dae Youn
    • Journal of Life Science
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    • v.27 no.9
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    • pp.975-985
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    • 2017
  • To investigate the beneficial effects of an agar gel mask (AGM) on UV-induced photoaging, SKH-1 hairless mice were treated with a topical application of AGM and an AGM dipped in essence (AGMdE). The mice were divided into an no radiation group, UV + AGM, UV + AGMdE, and UV + vehicle (PBS) treatment groups. Alterations in skin wrinkles, skin phenotype, histological structures, oxidative status, and toxicity were then evaluated during 4 weeks of exposure. The topical application of AGM and AGMdE inhibited wrinkle formation, suppressed the erythema index, prevented transepidermal water loss, and enhanced skin hydration. In addition, epidermal thickness recovered to a similar level as that in the no irradiation group in the UV + AGM and UV + AGMdE treatment groups compared with the UV + vehicle (distilled water) group. Furthermore, the expression levels of matrix metalloproteinase-1 (MMP-1) and tyrosinase were reduced in the UV + AGM and UV + AGMdE treatment groups, although the highest level varied. Moreover, superoxide dismutase (SOD) activity was significantly lower in the UV + AGM and UV + AGMdE treatment groups as compared with the UV + vehicle group. No significant alterations induced by most toxic compounds were measured in serum biochemical markers and liver and kidney histological features of the UV + AGM and UV + AGMdE treatment groups. These results suggest that AGM may protect against skin aging by regulating skin morphology, histopathological structures, and oxidative conditions.

Impact of Elevated Temperature and CO2 on Growth and Fruit Quality of Pepper (Capsicum annuum L.) (온도 및 CO2 상승이 고추의 생육 및 과실품질에 미치는 영향)

  • Song, Eun Young;Moon, Kyung Hwan;Son, In Chang;Wi, Seung Hwan;Kim, Chun Hwan;Lim, Chan Kyu;Oh, Soonja
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.18 no.4
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    • pp.179-187
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    • 2016
  • This study was conducted to determine the impact of elevated temperature and $CO_2$ concentration based on climate change scenario on growth and fruit quality of pepper (Capsicum annuum L. cv. Muhanjilju) with SPAR (Soil Plant Atmosphere Research) chamber. The intraday temperatures of climate normal years fixed by $20.8^{\circ}C$ during the growing season (May 1~October 30) of climatic normal years (1971~2000) in Andong region. There were treated with 4 groups such like a control group (ambient temperature and 400ppm $CO_2$), an elevated $CO_2$ group (ambient temperature and 800ppm $CO_2$), an elevated temperature group (ambient temperature+$6^{\circ}C$ and 400ppm $CO_2$) and an elevated temperature/$CO_2$ group (ambient temperature+$6^{\circ}C$ and 800ppm $CO_2$). Compared with the control, plant height, branch number and leaf number increased under the elevated temperature and elevated temperature/$CO_2$ group. However, leaf area and chlorophyll content showed a tendency of decreasing in the elevated temperature group and elevated temperature/$CO_2$ group. The number of flower and bud were decreased in the elevated temperature and elevated temperature/$CO_2$ group (mean temperature at $26.8^{\circ}C$) during the growth period. The total number and the weight of fruits were decreased in the elevated temperature group and elevated temperature/$CO_2$ group more than the control group. While the weight, length and diameter of fruit decreased more than those of control as the temperature and $CO_2$ concentration increased gradually. This result suggests that the fruit yield could be decreased under the elevated temperature/$CO_2$ ($6^{\circ}C$ higher than atmospheric temperature/2-fold higher than atmospheric $CO_2$ concentration), whereas the percentage of ripen fruits after 100 days of planting was increased, and showed earlier harvest time than the control.

Preparation of $SrTiO_3$ Thin Film by RF Magnetron Sputtering and Its Dielectric Properties (RF 마그네트론 스퍼터링법에 의한 $SrTiO_3$박막제조와 유전특성)

  • Kim, Byeong-Gu;Son, Bong-Gyun;Choe, Seung-Cheol
    • Korean Journal of Materials Research
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    • v.5 no.6
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    • pp.754-762
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    • 1995
  • Strontium titanate(SrTiO$_3$) thin film was prepared on Si substrates by RF magnetron sputtering for a high capacitance density required for the next generation of LSTs. The optimum deposition conditions for SrTiO$_3$thin film were investigated by controlling the deposition parameters. The crystallinity of films and the interface reactions between SrTO$_3$film and Si substrate were characterized by XRD and AES respectively. High quality films were obtained by using the mixed gas of Ar and $O_2$for sputtering. The films were deposited at various bias voltages to obtain the optimum conditions for a high quality file. The best crystallinity was obtained at film thickness of 300nm with the sputtering gas of Ar+20% $O_2$and the bias voltage of 100V. The barrier layer of Pt(100nm)/Ti(50nm) was very effective in avoiding the formation of SiO$_2$layer at the interface between SrTiO$_3$film and Si substrate. The capacitor with Au/SrTiO$_3$/Pt/Ti/SiO$_2$/Si structure was prepared to measure the electric and the dielectric properties. The highest capacitance and the lowest leakage current density were obtained by annealing at $600^{\circ}C$ for 2hrs. The typical specific capacitance was 6.4fF/$\textrm{cm}^2$, the relative dielectric constant was 217, and the leakage current density was about 2.0$\times$10$^{-8}$ A/$\textrm{cm}^2$ at the SrTiO$_3$film with the thickness of 300nm.

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Development of in-situ Sintered Ni-Al Alloy Anode for Molten Carbonate Fuel Cell (용융탄산염 연료전지용 in-situ 소결된 Ni-Al 합금 연료극 개발)

  • Chun, H.A.;Yoon, S.P.;Han, J.;Nam, S.W.;Lim, T.H.
    • Journal of the Korean Electrochemical Society
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    • v.9 no.3
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    • pp.124-131
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    • 2006
  • For commercialization of molten carbonate fuel cell (MCFC), it has some problems to be overcome such as decrease of porosity and thickness of the anode under the operating condition (at $650^{\circ}C$ and working pressure of more than 2 $kg_f/cm^2$). Recently, Ni-Al alloy anode has been proposed to replace the conventional Ni-Cr anode as an alternative material to resist a creep and inhibit the sintering. The objective of this research is to sinter the green sheet of Ni-Al alloy anode during single cell pre-treatment process, which has several advantages like cost down and simplification of manufacturing process. However, the Ni-Al alloy anode prepared with a conventional pre-treatment process showed the phase separation of Ni-Al alloy and formation of micropore(${\leqq}0.4{\mu}m$), resulting in low creep resistance and high electrolyte re-distribution. In order to prevent the Ni-Al alloy anode from phase-separating, nitrogen gas was used in the process of pre-treatment. Introducing the nitrogen, the phase separation from Ni-Al alloy into nickel and alumina was minimized and increased creep resistance. However, there was some micropore formation on the surface of Ni-Al alloy anode during the cell operation due to creation of lithium aluminate. Addition of more amount of electrolyte into a cell, especially at cathode, made the cell performance stable for 2,000 hrs. Consequently, it was possible to make the Ni-Al alloy anode with good creep resistance by the modified in-situ sintering technique.

Influence of additional etching on shear bond strength of self-etching adhesive system to enamel (부가적인 산부식이 자가산부식 접착제의 법랑질에 대한 전단결합강도에 미치는 영향)

  • Yoo Sun-Jin;Kim Young-Kyung;Park Jeong-Won;Jin Myoung-Uk;Kim Sung-Kyo
    • Restorative Dentistry and Endodontics
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    • v.31 no.4
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    • pp.263-268
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    • 2006
  • Recently, self-etching adhesive system has been introduced to simplify the clinical bonding proce- dures. It is less acidic compared to the phosphoric acid, thus there is doubt whether this system has enough bond strength to enamel. The purpose of this study was to investigate the influence of additional etching on the adhesion of resin composite to enamel. Ninety extracted bovine permanent anterior teeth were used. The labial surfaces of the crown were ground with 600-grit abrasive paper under wet condition. The teeth were randomly divided into six groups of 15 teeth each. Clearfil SE $Bond^{\circledR},\;Adper^{TM}$ Prompt L-Pop and Tyrian $SPE^{TM}$ were used as self-etching primers. Each self-etching primers were applied in both enamel specimens with and without additional etching. For additional etching groups, enamel surface was pretreated with 32% phosphoric acid (UNI-ETCH, Bisco, Inc., Schaumburg, IL. USA). Hybrid resin composite Clearfil AP-X, (Kuraray Co., Ltd., Osaka, Japan) was packed into the mold and light-cured for 40 seconds. Twenty-four hours after storage, the specimens were tested in shear bond strength. The data for each group were subjected to independent t - test at p < 0.01 to make comparisons among the groups. In Clearfil SE $Bond^{\circledR}$, shear bond strength of additional etching group was higher than no additional etching group (p < 0.01). In $Adper^{TM}$ Prompt L-Pop and Tyrian SPE, there were no significant difference between additional etching and non-etching groups (p > 0.01). In conclusion, self-etching adhesive system with weak acid seems to have higher bond strength to enamel with additional etching, while self-etching adhesive system with strong acid seems not.

Influence of Soil Salinity on the Growth Response and Inorganic Nutrient Content of a Millet Cultivar (토양염농도에 따른 기장의 생장반응 및 무기양분함량 변화)

  • Kim, Sun;Ryu, Jin-Hee;Kim, Young-Joo;Jeong, Jae-Hyeok;Lee, Su-Hwan;Oh, Yang-Yeol;Kim, Young-Doo;Kim, Jae-Hyen
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.61 no.2
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    • pp.113-118
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    • 2016
  • This experiment was conducted to identify the variations in inorganic nutrients and plant growth in millet (Panicum miliaceum L.) due to soil salinity. The soil series was Munpo and soil texture was silt loam. The experimental soil was amended so that the soil had salinities of $0.8dS\;m^{-1}$, $1.6dS\;m^{-1}$, $3.2dS\;m^{-1}$ and $4.8dS\;m^{-1}$. Millet was transplanted 15 days after sowing. As soil salinity increased, the degree of reduced growth was in the order of seed production > root dry matter > plant dry matter > culm length > tiller number > stem thickness > Panicle length. Seed production was decreased to 18.9% in soil salinity of $1.6dS\;m^{-1}$, 36.9% in of $3.2dS\;m^{-1}$, and 50.7% in EC of $4.8dS\;m^{-1}$. Root dry matter decreased to 35.8% in EC of $3.2dS\;m^{-1}$, and to 40.5% in EC of $4.8dS\;m^{-1}$. As soil salinity increased, Total nitrogen content increased in all aboveground parts, roots and seeds. However, There was no difference in CaO, $P_2O_5$, $K_2O$ and, MgO in soils of different salinity. On the other hand, $Na_2O$ content was higher in the order roots> shoots> seed, and in the case of roots, $Na_2O$ content increased to 1.02% in soil salinity of $4.8dS\;m^{-1}$. However, up to soil salinity of $1.6dS\;m^{-1}$, the $Na_2O$ content of the seed was similar to that in plant grown in the Control conditions($0.8dS\;m^{-1}$). In conclusion, taking into consideration economic factors, millet could be cultivated in soil with salinities of up to approximately $1.6dS\;m^{-1}$, and seed produced from reclaimedland would be suitable for human consumption.

Ecological Characteristics and Yield of Major Soybean Cultivars at Different Sowing Times in Southern Korea (남부지역에서 주요 콩 품종의 파종기에 따른 생태적특성 및 수량 변이)

  • Kim, Dong-Kwan;Choi, Jin-Gyung;Park, Heung-Gyu;Shin, Hae-Ryong;Yoon, Seong-Tak;Lee, Kyung-Dong;Rim, Yo-Sup
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.1
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    • pp.57-66
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    • 2013
  • The purpose of this study was to investigate the effects of shifts in sowing time on the ecological responses, growth and yields of major soybean cultivars in a southern region of South Korea. Experiments were carried out in Naju, Jeonnam Province (latitude $35^{\circ}$ 04'N, longitude $126^{\circ}$ 54'E) for three years from 2008 to 2010. The test cultivars included Saeol-kong and Tawon-kong of the summer type, Taekwang-kong and Pungsannamul-kong, and Cheongja3 of the autumn type. Sowing took place on May 15 and 30, June 15 and 30, and July 15 of each year. Of the summer type soybean cultivars, Saeol-kong showed a smaller curtailment in days from sowing to flowering, days from flowering to maturity, and days from sowing to maturity according to sowing time postponement than Tawon-kong. Of the autumn type soybean cultivars, Taekwang-kong exhibited a lower photoperiodic response in reproductive growth period than Pungsannamul-kong and Cheongja3, both of which recorded higher level photoperiodic responses in vegetative growth stages and reproductive growth periods than other test cultivars, with the former exhibiting higher levels than the latter. Most of the test cultivars tended to decrease in stem length, node numbers of the main stem, and stem diameter according to postponed sowing time, but there were no significant differences in stem length and node numbers of the main stem of the Saeol-kong cultivar. Differences in sowing times did not affect the first setting pod node order of Saeol-kong and Tawon-kong summer type cultivars of internode lengths of all of the test cultivars. All of the test cultivars tended to show decreases in pod number per plant due to postponement of sowing time except for the Saeol-kong cultivar. The variation was more prominent in small grain cultivars such as Tawon-kong and Pungsannamul-kong with the latter autumn type cultivar showing especially large variation. Yields were the greatest for the Tawon-kong, Taekwang-kong, and Pungsannamul-kong cultivars sown on May 30 and Cheongja3 sown on May 30 and June 15. There were no significant differences in the yields of Saeol-kong for different sowing times from May 30 to July 15, with the yields lowest for the batch sown on May 15.