• Weed & Turfgrass Science
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• v.2 no.3
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• pp.298-305
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• 2013

Soldier fly casts produced by eco-friendly treatment of food waste with American soldier fly was composting cast mixed food waste compost and soldier fly pupa. This study was conducted to evaluate a possibility of soldier fly casts (SFC) as soil amendment by investigating soil properties and zoysiagrass growth. Amendments were selected coco peat, compost and SFC and their ratio mixed with sand were non-amendment (NA), 7% coco peat(Coco), 7% compost (Comp), 3.5% SFC (1/2SFC) and 7% SFC. In soil properties, SFC was increased CEC than NA and T-N, Av-$P_2O_5$ and exchangeable K in soil than Coco. Compared with Coco and Comp, turf quality such as turf color index and chlorophyll index in SFC was higher and root length of zoysiagrass longer. Growth of shoot, runner and root in SFC was increase than that of Coco and Comp. These results indicated that 7% SFC applied as soil amendment was improved growth and quality of zoysiagrass by prompting soil productivity and nutrient availability in soil and root growth.

• Journal of Life Science
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• v.32 no.8
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• pp.601-610
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• 2022

P. tenebrifer (PT) belongs to the Diptera order and Stratiomyidae family. Recently, insect industry have been focused as food, animal feed and environmental advantages. γ-aminobutyric acid (GABA) and melatonin have been associated with regulating sleep and depression. GABA is the primary inhibitory neurotransmitter and is synthesized via biotransformation of monosodium glutamate (MSG) to GABA by lactic acid bacteria. In this study, we first used a GABA-enhanced PT extract, wherein GABA was enhanced by feeding MSG to PT. The underlying mechanisms preventing stress and insomnia were investigated in a corticosterone (CORT)-induced endoplasmic reticulum (ER) stress and chronic restraint stress (CRS)-exposed mouse model, as well as in pentobarbital (45 mg/kg)-induced sleep behaviors in mice. In the present study, the GABA peak was detected in high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) analysis and showed in Ptecticus tenebrifer water extract (PTW) but not in non-PTW extract. The results showed that PTW and Ptecticus tenebrifer with 70% ethanol extract (PTE) exerted neuroprotective effects by protecting against CORT-induced downregulation of phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2) and cAMP-response element binding protein (CREB) expression. In addition, PTW (300 mg/kg) significantly reduced CORT levels in CRS-exposed mice. Furthermore, PTW (100 and 300 mg/kg) significantly reduced sleep latency and increased total sleep duration in pentobarbital (45 mg/kg)-induced sleeping behaviors, which was related to serum melatonin levels. In conclusion, our results suggest that PTW exerts anti-stress and sleep-enhancing effects by regulating serum CORT and melatonin levels.

• 농업기술회보
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• v.46 no.5
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• pp.28-29
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• 2009

인삼은 본밭에 나가기 전에 모밭에서 1년(약 6개월)동안 모종을 음식물쓰레기를 분해하는 성능이 뛰어난 고충 "동애등에"이다. 농촌진흥청은 이 곤충을 대량 사육하는 기술을 개발하여 효과적인 음식물 처리는 물론 곧 곤충산업화가 이루어질 전망이다.

• Journal of the Korean Applied Science and Technology
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• v.36 no.4
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• pp.1399-1409
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• 2019

Biodiesel is a renewable and environmentally friendly liquid biofuel for transportation. Insect is considered as a new valuable biomass to convert into biodiesel. In particular, BSF(Black Soldier Fly) containing high fat is a renewable source of biodiesel. Biodiesel drived BSF has high concentration of saturated fatty acid methyl ester and low concentration of polyunsaturted fatty acid methyl ester which makes it potentially an ideal substrate for producing excellent quality biodiesel. Most of the fuel properties of BSF biodiesel were met the requirements of standard EN 14214. BSF have a higher lipid yield and biodiesel productivity as compared to microalgae and vegetable oils. This review paper includes the overall summary and compilation of the insect research conducted on biodiesel production and includes the BSF biodiesel properties.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.9
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• pp.255-261
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• 2019

This trial was conducted to determine the optimal supplementation level of Hermetia illucens (HI) on the growth performance, the apparent total tract retention of nutrients and the economic efficiency in weaning pigs. A total of 192 weaned pigs ($Landrace{\times}Yorkshire{\times}Duroc$; $6.51{\pm}0.15kg$) were randomly allotted to 4 treatments and 6 replicates on the basis of their initial body weight. The dietary treatments included a corn-soybean meal based diet supplemented with 0, 1, 2 or 3% HI. There were no significantly effects between the treatments in phases I, II and overall. But average daily gain was significant and linearly increased when HI was added to the diet in the overall group. Although digestibility of dry matter (DM) was increased by adding HI, digestibility responses were not observed by supplying HI in phase I. Also, all the parameters of economic efficiency were not significantly different according to the level of HI. The results showed that HI had beneficial effects on growth performance and digestibility of dry matter. However, there were no effects on economic efficiency by using HI in weaning pig diets.

• Journal of Life Science
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• v.31 no.12
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• pp.1094-1099
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• 2021

Hermetia illucens (Black soldier fly) is attracting attention as an environmental purification insect because it can supply a wide range of by-products of the agricultural food industry. Also, it has a potential feed for fish, birds, and pets due to a short life cycle and excellent nutritional components. Several pharmacological effects, including antimicrobial, of H. illucens have been reported. However, no study has focused on antiobesity effects of ethanol extract of H. illucens. In this study, we aimed to assess the anti-obesity effects of ethanol extract of H. illucens larvae (HIE) through inhibition of differentiation of 3T3-L1 preadipocytes into adipocytes. The amount of lipid accumulated in adipocytes was measured by oil red-O staining, and the inhibitory effect on adipogenesis was confirmed. The expression levels of factors related to adipocyte differentiation and fat synthesis were determined using Western blot analysis. Lipid droplet formation in adipocytes was remarkably inhibited by HIE. In addition, treatment with 400 ㎍/ml of HIE significantly reduced the expression levels of peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α-transcription factors involved in adipocyte differentiation. Therefore, the results of this study indicate that HIE is a potential anti-obesity agent because it inhibits adipocyte differentiation.

• Korean journal of applied entomology
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• v.52 no.3
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• pp.227-237
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• 2013

The black soldier fly (BSF), Hermetia illucens, is a generalist detritivore that is usually exploited for recycling environmental wastes, including food remnants. These insects can also be used to produce antibiotics for medical applications. For this purpose, flies need to be mass-reared using an automatic rearing system that generates various sounds. Insects use sound as a means of communication for mating or warning against predators. Thus, extrinsic sounds may interrupt the normal physiological processes of insects. In this study, we analyzed the physiological changes due to various sound treatments at $25^{\circ}C$ and 8:16 h (L:D) photoperiod. At 95 dB, larvae were exposed to different sound frequencies (0-5,000 Hz). The treated larvae were adversely affected by the high-frequency sounds and showed significant reduction in pupation and adult emergence. Different sound intensities (0-105 dB) at 5,000 Hz caused significant changes in the pupation and survival of the adults at 85 dB or above. Under a stress sound condition (5,000 Hz, 95 dB), the BSF larvae exhibited aberrations in the expression of genes related to the immune system and digestion. These results indicate that high-frequency sound treatments at or above 85 dB act as a stress sound to BSF development.

• Journal of Life Science
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• v.26 no.12
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• pp.1409-1414
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• 2016

In the larvae of the black soldier fly, Hermetia illucens, innate immunity mechanisms are activated in response to various pathogens and stimulants, resulting in the expression of antimicrobial peptides (AMPs). To induce the mass production of AMPs, H. illucens fifth instar larvae were immunized with five different kinds of bacteria. We isolated from the hemolymph of the H. illucens larvae after bacterial challenge, and their antimicrobial activities against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) were measured using the inhibition zone assay. Among these five different kinds of bacteria, the hemolymph of Bacillus subtilis-challenged H. illucens larvae showed the strongest antimicrobial activity against both Gram-positive bacteria and Gram-negative bacteria. The antimicrobial activity of the hemolymph of $1{\times}10^9cfu/ml$ B. subtilis-challenged H. illucens peaks at 24 hr at 48 hr post-infection and gradually declines with time. Moreover, the immunized hemolymph also showed strong antimicrobial activity against various poultry pathogens such as S. enteritidis, S. typhimurium, and S. pullorum. These results suggest that the expression of AMP genes in B. subtilis-challenged H. illucens is up-regulated by innate immune responses, and that B. subtilis-challenged H. illucens overexpressing AMPs may be useful as a feed additive in livestock diets to reduce the need for antibiotics.

• Journal of Sericultural and Entomological Science
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• v.51 no.1
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• pp.30-35
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• 2013

This experiment was conducted to investigate the effect of dietary supplementation of the black soldier fly (bsf) larvae on growth performance, carcass rate, and fatty acid profile of chicken meat in broiler chicks. Four hundred eighty 1-d-old broilers (Ross 308) were distributed into 3 treatments with 4 pens at 30 birds per replicate. Treatments were corn-soybean meal based broiler diet (control) and the basal diet replaced with bsf at the level of 3 and 6% respectively. Viability was not different significantly. Body weight gain was higher in control than other treatments. Feed intake was not statistically different of treatments. Feed conversion ratio was lower in control than other treatments. Relative weight of breast meat was significantly increased in all treated groups than in control. But, another relative weights of chicken meat cuts (thigh, wing, neck, back) were not significantly different among the groups. In conclusion, it could be suggested that dietary supplementation of black soldier flylarvae could beneficially improved the performance on broiler.

• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.107-113
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• 2013

The black soldier fly larvae are able to decompose various organic wastes such as livestock manures and food wastes. We tested whether the quality of the insect derived compost, i.e. larval feces, was comparable to that of a commercial fertilizer. Chemical analysis of Hermetia. illucens compost was suitable as a fertilizer. When the kidney bean (Phaseolus vulgaris L.) was raised on the culture soil treated with the H. illucens compost, the growth of leaf area, total dry weight and leaf dry weight increased significantly. The H. illucens compost is thought to act as an increasing factor of RGR (relative growth rate) from the beginning of growth and also had a great effect on the relative growth rate throughout the late stage of growth. There is high statistical significance between NAR (net assimilation rate) and RGR (relative growth rate), but no significance between RGR and LAR (leaf assimilation rate) of the treatments. In addition, the treatment of H. illucens compost promoted the thickness of leaves from the beginning of growth and the tendency of thickening leaves from the beginning of growth and the effect continued throughout the late period of growth. When mixed with 50% of H. illucens compost rather than 25%, it showed the greater effect on the plant growth.