• Korean Journal of Animal Reproduction
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• v.16 no.3
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• pp.193-198
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• 1992

본 실험은 ICR계통 생쥐의 8세포배 및 상실배를 Vitrification(VS3) 동결보존액을 이용하여 초급속동결-융해를 실시하여 배반포배까지의 체외발생한 배반포배를 이식하였을 때 수태율에 미치는 영향을 조사하여 다음과 같은 결과를 얻었다. 1. 8세포배의 평형시간(3분 및 6분)에 따른 초급속 동결-융해후 배반포배까지의 체외발생율은 57.6% 및 59.5%여TEk. 2. 상실배의 평형시간(3분 및 6분)에 따른 초급속 동결-융해후 배반포배까지의 체외발생율은 64.4% 및 68.2%였다. 3. 8세포배와 상실배를 초급속 동결-융해를 실시하여 배반포배까지 체외발생한 배를 위임신되어진 수란쥐에 19개 및 20개를 이식하여 7필(36.8%)과 10필(50.0%)의 산자를 얻었다.

• Proceedings of the Korea Concrete Institute Conference
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• 2009.05a
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• pp.21-22
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• 2009

This paper presents the results of an experimental study on flexural behavior and cracking process of concrete beams subjected to cycles of freezing and thawing after patch-repaired with strain-hardening cement composites (SHCCs). The SHCCs were reinforced with hybrid 0.75% PVA and 0.75% PE fibers. Experimental testing of concrete beams patch-repaired with SHCCs revealed that the SHCC patch-repair system without freeze thaw (FT) exposure showed average 3.31 times increased load carrying capacity and for beams exposed to 300 FT cycles, load carrying capacity increased up to 2.42 times. Cracking damage of SHCC patch-repaired beams mitigated compared to plain concrete beams but this trend decreased under FT exposure.

• Magazine of the Korea Concrete Institute
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• v.10 no.1
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• pp.125-132
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• 1998

경량골재 콘크리트의 내구성과 경제성에 대한 인식 부족으로 조경재료나 인공토양 둥 구조부재 이외의 분야에 사용되고 있는 국내 실정에 비해서, 구미 여러나라에서는 고강도 경량골재를 장지간 교량과 고충건물에 사용하고 있다. 경량골재 콘크리트는 구조물의 재료비 단순비교에 있어서도 경제성이 있을 뿐만 아니라, 자중감소로 인한 구조적, 기하학적 장점도 있으며, 또한 고강도 경량골재의 개발로 경량골재가 가지고 있는 여러 문제점을 해소하여 사용성과 내구성에 있어서 보통골재 콘크리트와 큰 차이가 없는 상황이다. 그러나국내에서 생산된 경량골재는 닫힌 공극보다 열린 공극을 많이 내포하고 있어 수분흡수가 많고, 특히, 동결융해에 대한 내구성에 취약한 문제점을 가지고 있다. 본 논문에서는 내동해성 향상을 위해 10종류의 고강도 경량골재 콘크리트 공시체를 제작하여 실리카 흄, 물.시멘트비, AE제, 강섬유 등을 실험 변수로 하여동결융해 실험을 수행하였다. 연구결과 실리카흄, 물.시멘트 비는 어느 정도 내동해성을 향상시키지만 근본적인 해결방안이 되지 못하며, AE제를 첨가한 공시체와 강섬유를 사용한 공시체는 동결융해 내구성 지수가 90%이상으로 측정되어 내동해성을 개선시킬 수 있는 요소로 나타났다.

• Journal of the Korean Geotechnical Society
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• v.39 no.11
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• pp.53-62
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• 2023

The characteristics of unfrozen water content in frozen soils significantly impact the thermal, hydraulic, and mechanical behavior of the ground. A thorough analysis of the unfrozen water content characteristics of the target subsoil material is crucial for evaluating the stability of frozen ground. This study conducted indoor experiments to measure the freezing point and unfrozen water content of sandy soil while considering pore water salinity. Utilizing the experimental data, we introduced a novel empirical model to conveniently estimate the unfrozen water retention curve. Furthermore, the validity of the unfrozen water retention curve was assessed by comparing the experimental data with the results of a simulation model that utilized the proposed empirical model as input data.

• Development and Reproduction
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• v.2 no.1
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• pp.63-68
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• 1998

This study was done to find optimal cryopreservation medium and method to improve the post-thaw motility of human sperm. Thirty three semaen samples were included in the study. Of these, nineteen Samples showing normal semen profile were frozen using three cryprotectants (TYB, TYB+DTT and KS II)to compare post-thaw motility. Fourteen samples were frozen with vapor freezing and programmable freezing methods to compare post-thaw motility in correlation with freezing method. After 24 hrs of cryostorage , the vials were thawed and the post-thaw sperm motility was assessed by two kinds of computer-aededsperm analysis (CASA; SAIS and Hamilton Thorn). As a result, the post-thaw motility of the KS II group was higher than the TYB or TYB+DTT group in normal semen(34.8%, 28.3% and 23.0%, respectively). In the asthenospermia group, a significantly hither post-thaw motility was observed in the KS II (18.5%) compared to those of TYB or TYB+DTT group (13.6%, 10.0%, respectively). No difference was observed between vapor freezing group and computerized freezing group in normal semen (27.8%, 33.2%, respectively) and semen group showing asthenospermia (12.8%, 12.9%, respectively). In conclusion, our results indicate that KS II medium is reliable for cryopreservation of human sperm and vapor freezing and programmable freezing were equally effective in terms of the recovery of motile spermatozoa.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.1097-1100
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• 2008

The purpose of this study to investigate the bond strength of recycled coarse aggregate concrete deteriorated by freezing and thawing. Concrete specimens with recycled coarse aggregate representing lower limit of the quality standard (water absorption : 3.0%, specific gravity : $2.5g.cm^3$) were manufactured and tested. The replacement ratio (0, 30, 60 and 100%) of recycled coarse aggregate and freezing-thawing cycles were considered in this test. From the test results, it was found that the bond strength of normal strength concrete is not affected by the replacement ratio of recycled coarse aggregate under freezing and thawing conditions. Also, the bond strength of the natural and recycled coarse aggregate concrete using AE admixtures was not decreased by frost action.

• Journal of the Korean Geotechnical Society
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• v.27 no.9
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• pp.67-76
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• 2011

Bearing capacity of pile foundations in cold region is dominated by adfreeze bond strength between surrounding soil and pile perimeter. Adfreeze bond strength is considered to be the most important design parameter for foundations in cold region. Many studies in last 50 years have been conducted to analyze characteristics of adfreeze bond strength. However, most studies have been performed under constant temperature and normal stress conditions in order to analyze affecting factors like soil type, pile material, loading speed, etc. In this study, both freezing temperature and normal stress acting on pile surface were considered to be primary factors affecting adfreeze bond strength, while other factors such as soil type, pile material and loading speed were predefined. Direct shear box was used to measure adfreeze bond strength between Joomoonjin sand and aluminium because it is easy to work for various roughness. Test was performed with temperatures of > $0^{\circ}C$, $-1^{\circ}C$, $-2^{\circ}C$, $-5^{\circ}C$, and $-10^{\circ}C$ and vertical confining pressures of 1atm, 2atm, and 3atm. Based on the test results, the effects of temperature and vertical stress on adfreeze bond strength were analyzed. The test results showed that adfreeze bond strength increases with decreased temperature and increased vertical stress. It was also noted that two types of distinct sections exist, owing to the rate of increase of adfreeze bond strength along the change of freezing temperature: 1)rapidly increasing section and 2)gradually decreasing section. In addition, the results showed that a main factor affecting adfreeze bond strength switches from friction angle to adhesion as freezing temperature decreases.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.1D
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• pp.55-61
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• 2009

In this research freezing depth analyses were performed for the recent 9 project field sites using FrostAM which is a freezing analysis model developed recently within the country and a model based on regional environmental factors and physical properties of pavement materials. The environmental factors needed for freezing depth analysis were obtained from the meteorological agency website. And there were laboratory tests and analyses using a measuring device for properties of unsaturated soil for the field site samples across country to obtain hydrographical properties among physical properties. The freezing depths analyzed by FrostAM were deeper in the range of 14cm~44cm than those based on freezing index. It is considered that the freezing depths based on freezing index were overestimated. And there are considerable differences among the freezing depths based on freezing index which were designed by different designers.

• Reproductive and Developmental Biology
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• v.30 no.1
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• pp.59-64
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• 2006

This study was conducted to establish a convenient freezing method of boar semen. Boar semen was cooled until $5^{\circ}C$ for 3 hrs using cell freezer and loaded into straws. Semen straws were frozen in different steps in strofoam box filled with $LN_2$. Highest sperm viability (54.0%) was obtained by 1-step freezing(holding at 10 cm height from the surface of $LN_2$ for 10 min). Sperm viability increased by holding at $-102^{\circ}C$ for 10min (74.0%, P<0.05). In thawing regime, sperm viability was significantly higher in $37^{\circ}C$ group than in $52^{\circ}C$ group. The sperm characteristics did not differ between 1-step and 3-step. After IVF using frozen-thawed boar semen, developmental rate of embryos to the morula+blastocyst stage was in 1-step freezing group than that of 3-step freezing group (27.5 vs 14.7%, P<0.05). The result shows that the 1-step freezing with holding at $-102^{\circ}C$ for 10min before plunging into $LN_2$ is a convenient and easy freezing method for boar semen.

• Korean Journal of Ichthyology
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• v.31 no.4
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• pp.195-200
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• 2019

An experiment was performed to obtain cryopreservation techniques of Pacific cod Gadus macrocephalus sperm. Milt were cryopreservation using five cryoprotectant demethyl sulfoxide (DMSO), ethylene glycol (EG), glycerol, methanol and propylene glycol (PG) with marine fish ringer's solution (MFRS) as diluent. Milt were cryopreserved each experimental methods like cryoprotectants (10% and 20%), equilibration time (3, 5 and 10 min) and freezing protocols (liquid nitrogen vapor above 3, 8 and 12 cm). Post-thaw sperm survival rate revealed the highest in 10% PG with optimum methods of equilibration time (3 min) and freezing protocol (liquid nitrogen vapor above 8 cm) about 21.3±1.8%. Hatching rate of fertilization eggs using fresh and cryopreserved sperm were no significantly different.