Browse > Article

Establishment of the Convenient Boar Semen Freezing Method and Assessment of Viability in Frozen/Thawed Boar Semen  

Kim Seong-Kon (College of Animal Life Sciences, Kangwon National University)
Jang Hyun-Yong (College of Animal Life Sciences, Kangwon National University)
Park Dong-Heon (College of Animal Life Sciences, Kangwon National University)
Park Chun-Keun (College of Animal Life Sciences, Kangwon National University)
Cheong Hee-Tae (School of Veterinary Medicine, Kangwon National University)
Kim Choung-Ik (College of Animal Life Sciences, Kangwon National University)
Yang Boo-Keun (College of Animal Life Sciences, Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.30, no.1, 2006 , pp. 59-64 More about this Journal
Abstract
This study was conducted to establish a convenient freezing method of boar semen. Boar semen was cooled until $5^{\circ}C$ for 3 hrs using cell freezer and loaded into straws. Semen straws were frozen in different steps in strofoam box filled with $LN_2$. Highest sperm viability (54.0%) was obtained by 1-step freezing(holding at 10 cm height from the surface of $LN_2$ for 10 min). Sperm viability increased by holding at $-102^{\circ}C$ for 10min (74.0%, P<0.05). In thawing regime, sperm viability was significantly higher in $37^{\circ}C$ group than in $52^{\circ}C$ group. The sperm characteristics did not differ between 1-step and 3-step. After IVF using frozen-thawed boar semen, developmental rate of embryos to the morula+blastocyst stage was in 1-step freezing group than that of 3-step freezing group (27.5 vs 14.7%, P<0.05). The result shows that the 1-step freezing with holding at $-102^{\circ}C$ for 10min before plunging into $LN_2$ is a convenient and easy freezing method for boar semen.
Keywords
Frozen/thawed boar semen; Acrosome intactness; Semen quality; Embryo development;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Almlid T, Hofmo PO (1996): Brief review of frozen semen application under Norwegian AI service conditions. Reprod Dom Anim 31:169-173   DOI   ScienceOn
2 Hammersted RH, Graham JK, Nolan JP (1990): Cryopreservation of mammalian sperm : What we ask them to survive. J Androl 11:73-88
3 Medrano A, Watson PF, Holt WV (2002): Importance of cooling rate and animal variability for boar spenn cryopreservation : Insights from the cryomicroscope. Reproduction 123:315-322   DOI
4 Watson PF (1996): Cooling of spennatozoa and fertilizing capacity. In Rath D, Johnson LA and Weitze KF (eds), boar semen preservation III. Proc. 3rd Int. Cont. Boar semen preservation, Mariencee, Gennany, August, 1995. Reprod Domest Anim Vol 31 Blackwell, Berlin, pp. 135-140
5 Neild DM, Gadella BM, Maria GC, Marcelo H, Miragaya C, Colenbrander B, Aguero A (2003): Membrane changes during different stages of a freeze-thaw protocol for equine semen cryopreservation. Theriogenology 59:1693-1705   DOI   ScienceOn
6 Nagai T, Takahashi T, Masuda H, Shioyo Y, Kumayama M, Fukushima M, Iwasaki S, Hanada A (1988): In vitro fertilization of pig oocytes by frozen boar spennatozoa. J Reprod Fertil 84:585-591   DOI   ScienceOn
7 加藤碇史良, 井上揚一, 贋野森, 入浴明, 西川義正 (1976): Effect of thawing procedure on motility and acrosome system of frozen boar spermatozoa. 日本凍結精液硏究學會報48:15-19
8 Bwanga, CO (1991):. Cryopreservation of boar semen. I : A literature review. Acta Vet Scand 32: 431-453
9 Cordova A, Ducolomb Y, Jimenez I, Cassas E, Bonilla E, Betrancourt M (1997): In vitro fertilizing capacity of frozen-thawed boar semen. Theriogenology 47:1309-1317   DOI   ScienceOn
10 Maxwell WMC, Johnson LA (1997): Membrane status of boar spennatozoa after cooling or cryopreservation. Theriogenology 45:209-219   DOI
11 이광원, 지설하, 손동수, 김학규, 박창식, 김친철, 최진성, 고문석, 정행기, 김현 (1989): 5 m1 straw에 보존한 돼지 희석정 액의 생존성과 수정능력에 관한 연구. 한국동물자원과학회지 31:158-161
12 Eriksson BM, Petersson H, Rodriguez-Martinez H (2002): Field fertility with exported boar semen frozen: in .the new FlacPack container. Theriogenology 58:1065-1079   DOI   ScienceOn
13 Zheng YS, Fiser P, Sirard MA (1992): The use of ejaculated boar semen after freezing in 2 or 6% glycerol for in vitro fertilization of porcine oocytes matured in vitro. Theriogenology 38:1065-1075   DOI   ScienceOn
14 임종우, 윤창현 (1973): 돈정액 보존에 관한 연구. 축산진흥연구소보 1:96-102
15 최영진, 박춘근, 정희태, 김정익, 박동헌, 장현용, 양부근 (2002): 항산화제와 Growth factors 첨가가 돼지 체외 수정란의 체외발육에 미치는 효과. Korean J Anim Reprod 26:143-151
16 Huang SY, Kuo YH, Lee WC, Tsou HL, Lee YP, Chang HL, Li-Jun Huo JJ, Ma XH, Yang ZM (2002): Assessment of spenn viability, mitochondrial activity, capacitation and acrosome intactness in extended boar semen during long-tenn storage. Theriogenology 58:1349-1360   DOI   ScienceOn
17 Salisbury GW, VanDemark NL, Lodge JR (1978): Physiology of reproduction and artificial insemination of cattle. W. H. Freeman and Co. 2nd (eds), San Francisco, USA
18 정영호, 서경덕, 김광식, 심금섭, 이장희 (1999): 동결 보존한 돼지정액의 융해조건이 정자의 생존율과 첨체변화에 미치는 효과. 한국수정란이식학회지 14:131 -137
19 Ahmad K (1984): Effect of thaw rates on survival of buffalo spennatozoa frozen straws. J Dairy Sci 67:1535-1538   DOI   ScienceOn
20 Kumar S, Millar JD, Waston PF (2003): The effect of cooling rate on the survival of cryopreserved bull, ram, and boar spennatozoa : A comparison of two controlled-rate cooling machines. Cryobiology 46: 246-253   DOI   ScienceOn
21 Fiser PS, Rairfull RW, Marcus GJ (1986): The effect of thawing velocity on survival and acrosomal integrity of ram spennatozoa frozen at optimal and suboptimal rates in straws. Cryobiology 23:141-149   DOI   ScienceOn
22 Fiser PS, Fairfull RW (1990): Combined effect of .glycerol concentration and cooling velocity on motility ad acrosomal integrity of boar spennatozoa frozen in 0.5 ml straw. Mol Reprod Dev 25:123-129   DOI   ScienceOn