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http://dx.doi.org/10.35399/ISK.31.4.2

Sperm Cryopreservation of Pacific Cod Gadus macrocephalus  

Do, Yong Hyun (South East Sea Fisheries Research Institute, NIFS)
Cho, Jae Kwon (South East Sea Fisheries Research Institute, NIFS)
Lee, Hee Jung (South East Sea Fisheries Research Institute, NIFS)
Min, Byung Hwa (Aquaculture Research Division, NIFS)
Publication Information
Korean Journal of Ichthyology / v.31, no.4, 2019 , pp. 195-200 More about this Journal
Abstract
An experiment was performed to obtain cryopreservation techniques of Pacific cod Gadus macrocephalus sperm. Milt were cryopreservation using five cryoprotectant demethyl sulfoxide (DMSO), ethylene glycol (EG), glycerol, methanol and propylene glycol (PG) with marine fish ringer's solution (MFRS) as diluent. Milt were cryopreserved each experimental methods like cryoprotectants (10% and 20%), equilibration time (3, 5 and 10 min) and freezing protocols (liquid nitrogen vapor above 3, 8 and 12 cm). Post-thaw sperm survival rate revealed the highest in 10% PG with optimum methods of equilibration time (3 min) and freezing protocol (liquid nitrogen vapor above 8 cm) about 21.3±1.8%. Hatching rate of fertilization eggs using fresh and cryopreserved sperm were no significantly different.
Keywords
Pacific cod; sperm; cryopreservation; cryoprotectant;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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