• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.228-235
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• 2019

Apple (Malus pumila) is one of the most economically important fruits in Korea. but virus infection has decreased the sustainable production of apples and caused serious problems such as yield loss and poor fruit quality. Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. In many cases, as apple gets infected with virus and viroid with no specific symptoms, the damage and symptoms caused by the viruses are not detected. In our research, viruses in the rootstock were eliminated for a virus-free apple dwarfing rootstock of M.9 and M.26. The virus elimination methods were apical meristem culture, thermotherapy ($37^{\circ}C$, 6 weeks) and chemotherapy($Ribavirin^{(R)}$). The detection of apple viruses was accomplished by Enzyme-linked Immuno-Sorbent Assay (ELlSA) and reverse transcription-polymerase chain reaction (RT-PCR). RT- PCR method was 10 ~ 30% more sensitive than the ELISA method. The efficiency of virus elimination was enhanced in apical meristem culture method. The acquisition rate of virus-free apple dwarfing rootstocks was 30 ~ 40% higher in apical meristem culture. After the meristem culturing of M.9, the infection ratio of ACLSV, ASPV and ASGV was 45%, 60% and 50%, respectively. In the apple dwarfing rootstock of M.26, the infection ratio of ACLSV, ASPV and ASGV was 40%, 55% and 55%, respectively. Based on this study, the best method for the production of virus-free apple dwarfing rootstocks was the apical meristem culture.

• Journal of Bio-Environment Control
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• v.23 no.1
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• pp.43-49
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• 2014

This study was designed to determine the effect of red LEDs during healing and acclimatization process on the survival rate and quality of grafted tomato seedlings. Red LEDs and no light (darkness) were used for treating three rootstock cultivars, which are 'B-Blocking', 'Kanbarune', 'High-power' in healing room. Results showed that survival rates of grafted seedlings in red LEDs were higher than those in no light treatment. Significant variation on survival rates of rootstock cultivars was observed in no light treatment but there was not significant variation in red LEDs treatment. Light treatment also reduced the percentage of infected plants, except for the 'Kanbarune' cultivar. Seedling quality in red LEDs was better than that in no light treatment by improving growth parameters such as plant height, leaf length, leaf width, leaf area, fresh and dry weight of shoot and root. Light treatments and rootstock cultivars did not affect number of leaves, leaf chlorophyll value and T/R ratio of seedlings, but seedlings in red LEDs were significantly more compact than those in no light treatment. Moreover, the root morphology of seedlings such as total root surface area, total root length, and number of toot tips in red LEDs was also greater than that in no light treatment.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.11-17
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• 2007

Previously developed transgenic watermelon rootstocks (gongdae) inserted by CGMMV-CP were examined to test the virus tolerance levels. In the restricted plastic house, the $T_{3}$ watermelon rootstock showed tolerance to CGMMV until 70 days after inoculation on the leaves while the non-transformed watermelon rootstock became susceptible at 20 days after inoculation. In the field, tolerance efficiency of transgenic rootstocks maintained up to 40% at 71 days after contamination with CGMMV in the soil while all of the non-transformed rootstocks became susceptible at 37 days with the same condition. In the same field, transgenic rootstocks showed more tolerance to CGMMV than the non-transformed rootstocks as those were inoculated on the leaves, but it showed only 10 days delay before being susceptible. Therefore, transgenic rootstocks have a characteristic of delay effect against CGMMV susceptibility, rather than resistance character. From $T_{3}$ rootstocks homozygous for the CGMMV-CP horticulturally favorable individuals were selected for further breeding and a transgenic line was finally obtained at the $BC_{1}T_{5}$. A material transfer experiment was conducted to find out if the DNA, RNA or expressed protein in the transgenic rootstocks could move to the grafted scion (non-transformed watermelon, Super-Kumcheon). PCR, northern, and western blot analysis were performed and no evidence of transferring of those materials from rootstock to scion was ever found.

• Journal of Korean Society of Forest Science
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• v.40 no.1
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• pp.51-56
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• 1978

In the present study an experiment on the interspecific grafting of European and American filberst on the root stock of Korean filbert (C. heteropylla & C. sieboldiana) by means of veneer graftings in the green house was carried out and those survival percentage and growth was also investigated. The obtained results can be summarized as follows. 1. Interspecific grafting of European and American filberts on root stock of Korean filbert (C. heterophylla & C. sieboldiana) was 97%~98.3% of survival. 2. Those Interspecific grafts was higher compatibilities and nonsukering of root stocks of Korean filberts in the 1~2 years old. 3. Those mean growth of interspecific grafts was 106.7cm~111cm of height and 11.7mm~14.6mm of diameters at the stem base.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.1
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• pp.5-13
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• 2019

The first commercial planting of sweet cherry in Korea is thought to be done in early 1920s. The planting area of sweet cherry in Korea is about 500 ha in 2017. Although sweet cherry is considered a minor fruit in Korea, it is one of premium fruits in market and popular among children and women, especially among pregnant women. The import of sweet cherry fruits has increased dramatically in recent years. Seedlings of Prunus lannesiana or clones of 'Colt' (P. avium × P. pseudocerasus) were usually used as sweet cherry rootstocks in Korea. During recent decades new dwarfing rootstocks for cherry such as 'Gisela' series were developed in Germany. Among them, several 'Gisela' series have been mainly used in international nurseries. In this study, we investigated optimum rooting condition of a dwarfing rootstock 'Gisela 5' in summer season cuttings. Among eight soil conditions tested, saprolite + commercial nursery soil (1:1) and saprolite + vermiculite (1:1) showed higher rooting percentage than others, showing 93.6% and 88.9%, respectively. More than 95% of 'Gisela 5' rootstocks produce roots when it was treated with IBA for several seconds just before cuttings, irrespective of concentrations (500 to 2000 mg·L^-1).

• Journal of the Korean Society of Radiology
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• v.84 no.6
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• pp.1367-1372
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• 2023

Primary angiitis of the central nervous system (PACNS) is a rare vasculitis in the central nervous system. Herein, we report a case of diagnosis and treatment of necrotic pattern PACNS, which was difficult to differentiate from a brain abscess. A 19-year-old male presented with blurred vision and a headache. Brain MRI revealed irregular rim-enhancing necrotic masses with central diffusion-high signal intensity in the corpus callosum and peripheral diffusion-high signal intensity in the left parietotemporal periventricular area. Susceptibility-weighted imaging revealed multiple punctate hemorrhages in the lesions. The patient was diagnosed with unusual abscess or tumefactive PACNS. Therefore, we initially treated the patient with antibiotics to rule out brain abscess. However, the brain lesions did not improve on follow-up MRI after the antibiotic treatment. Surgical biopsy was performed, and the histopathological diagnosis was PACNS with a necrotic pattern. The necrotic lesions became smaller on follow-up MRI after high-dose corticosteroid treatment.

• 월간낙농육우
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• v.19 no.12 s.212
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• pp.94-95
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• 1999

연간 원유생산량 약 200만톤, 1998년 6월 1일부터 인하하였다고 치면 200만톤의 12분의 7인 117만톤, $5.4\% 인하시 톤당 가격은 29,036원이며 1998년에만도 약 340억원, 1999년도 약 580억원의 혜택을 본 셈이고, 이중에서 약 20억원을 각출하자는 것이었는데 무임승차자가 있었다는 것은 한번 깊이 반성해 보아야할 대목이 아닌가 싶다.

• 보건세계
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• v.63 no.1
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• pp.5-7
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• 2014

우리나라 인구의 약 1/3이 결핵에 감염된 것으로 알려져 있다. 많은 사람들이 이 대목에서 깜짝 놀란다. '국민 3명 중 1명이 감염자라고?' 감염이 곧 '결핵'을 의미하는 것은 아니다. 하지만 감염이 된 사람은 언제든 결핵으로 진전될 가능성을 안고 있는, 잠재적 결핵환자이다. 이 페이지에서는 내 몸에 결핵균이 들어왔는지 여부를 확인 할 수 있는 TST, 즉 투베르쿨린 피부검사에 대해 알아보기로 한다.

• 전기의세계
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• v.24 no.6
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• pp.18-23
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• 1975

대한전기학회로부터 기술해설에 관한 내용의 원고를 의뢰받고 주저했으나 결국은 착수하기로 했다. Multivariable function 이론이 개화하면 응용면에 있어 예측하기 어려울만큼 큰 장래가 약속된다고 믿어져 집필을 했다. 영문을 그대로 실은 대목도 있고 지면절약상 증명은 생략했다. 보완이 될까 생각되어 대표적인 논문들을 참고로 첨부한다. 큰 개발이 요구되는 이 분야에 우리 나라에서도 관심을 갖는 분이 많이 생겨 제기된 문제 해결에 공헌 있기를 바란다.

• Digital Contents
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• no.2 s.153
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• pp.81-81
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• 2006

게임 사용량이 증가하는 큰 요인 중 하나가 바로 기간적 요인이다. 방학이나 주말과 같은 특정기간이나 계절에는 게임 사용량이 증가한다는 것. 그중에서도 실내 활동 시간이 많아지는 겨울방학은 유저들의 게임사용 시간을 최대한 이끌어 내야하는, 그야말로 게임업체들의 대목이다. 2005년12월의 끝자락에서 2006년 새해 첫 달을 관통하는 겨울방학 황금기 동안 게임 사용량의 어떤 변화가 있었는지 살펴보았다.