Efficient virus elimination for apple dwarfing rootstock M.9 and M.26 via thermotherapy, ribavirin and apical meristem culture
![]() |
Kwon, Young Hee
(Chungcheongbuk-do Agricultural Research and Extension Services)
Lee, Joung Kwan (Chungcheongbuk-do Agricultural Research and Extension Services) Kim, Hee Kyu (Chungcheongbuk-do Agricultural Research and Extension Services) Kim, Kyung Ok (Chungcheongbuk-do Agricultural Research and Extension Services) Park, Jae Seong (Chungcheongbuk-do Agricultural Research and Extension Services) Huh, Yoon Sun (Chungcheongbuk-do Agricultural Research and Extension Services) Park, Eui Kwang (Chungcheongbuk-do Agricultural Research and Extension Services) Yoon, Yeo Joong (Uniplantech) |
1 | Campbell AI (1962) Apple virus inactivation by heat therapy and tip propagation. Nature 195: 520 DOI |
2 | Chellappan P, Vanitharani R, Ogbe F, Fauquet CM (2005) Effect of temperature on geminivirus-induced RNA silencing in plants. Plant Physiology 138:1828-1841 DOI |
3 | Cho KH, Shin JH, Kim DH, Park SJ, Kim SH, Chun JA, Kim MY, Han JH, Lee HC (2016) Elimination of Apple stem grooving virus from 'Mansoo' pear (Pyrus pyrifolia L.) by an antiviral agent combined with shoot tip culture. Journal of Plant Biotechnology 43:391-396 DOI |
4 | Cooper VC, Walkey DGA (1978) Thermal inactivation of Cherry leaf roll virus in tissue cultures of Nicotiana rustica raised from seeds and meristem tips. Annals of Applied Biology 88:273-278 DOI |
5 | Dixon-Holland DE and Katz SE (1991) Competitive direct enzyme-linked immunosorbent screening assay for the detection of sulfamethazine contamination of animal feeds. Journal Association of Analysis Chemistry International 74:784-789 |
6 | Paprstein F, Sedlak J, Polak J, Svobodova L, Hanssan M, Bryxiova M (2008) Results of in vitro thermotherapy of apple cultivars. Plant Cell Tissue Culture 94:347-352 DOI |
7 | Plopa C, Preda S (2013) Elimination of Apple mosaic virus by tissue culture of some infected apple cultivars. Acta Horticulture 981:517-522 DOI |
8 | Savitri WD, Park KI, Jeon SM, Chung MY, Han JS, Kim CK (2013) Elimination of Chrysanthemum stunt viroid (CSVd) from meristem tip culture combined with prolonged cold treatment. Horticulture Environment Biotechnology. 54:177-182 DOI |
9 | Smith PR, Challen D I (1972) Aetiology of the rosette and decline diseases of peach and interactions between Prunus necrotic ringspot, Prune dwarf, and dark green sunken mottle viruses. Australian Journal of Agriculture Research. 23:1027-1034 DOI |
10 | Tan R, Wang L, Hong N, Wang G (2010) Enhanced efficiency of virus eradication following thermotherapy of shoot-tip cultures of pear. Plant Cell Tissue and Organ Culture 101:229-235 DOI |
11 | Verma N, Ram R, Zaidi AA (2005) In vitro production of Prunus necrotic ringspot virus-free begonias through chemo-and thermotherapy. Science Horticulture 103:239-247 DOI |
12 | Knapp E, Hanzer V, Weiss H, da Camara Machado A, Wang Q, Weiss B, Katinger H, Laimer da Camara Machado M (1995) Distribution of Apple chlorotic leaf spot virus in apple shoots cultivated in vitro. Acta Horticuture 386:187-194 |
13 | Wang L, Wang G, Hong N, Tang R, Deng X (2006) Effect of thermotherapy on elimination of Apple stem grooving virus and Apple chlorotic leaf spot virus for in vitro-cultured pear shoot tips. Horticulture Science 41:729-732 |
14 | Wang QC, Cuellar WJ, Rajamaki ML, Hirata Y, Valkonen JPT (2008) Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Molecular Plant Pathology 9:237-250 DOI |
15 | Wood GA (1979) Virus and Virus-Like Diseases of Pome Fruits And Stone Fruits in New Zealand. No. 226. Bulletin, Department of Scientific and Industrial Research, Wellington. 87 |
16 | Kim MY, Chun JA, Cho KH, Park SJ, Kim SH, Lee HC (2017) Efficiency of virus elimination in apple callus (cv. Hongro) derived from meristem culture of dormant buds. Journal Plant Biotechnology 44:379-387 DOI |
17 | Kinard, G, Scott S, Barnett O (1996) Detection of apple chlorotic leaf spot and apple stem grooving viruses using RT-PCR. Plant Disease. 80: 616-621 DOI |
18 | KOSIS(Korea statistical Information Service), 2018 |
19 | Lee G, Kim JH, Kim HR, Shin IS, Cho KH, Kim SH, Shin J, Kim DH (2013) Production system of virus-free apple plants using heat treatment and shoot tip culture. Research in Plant Disease 19: 288-293 DOI |
20 | Lee SH, Kwon YS, Shin HM, Nam SY, Hong EY, Kim BK, Kim DE, Cha , and Jae-Soon Cha (2017) Survey on Virus Infection for Commercial Nursery Trees of Major Apple Cultivars in Korea. Research in Plant Disease. 23(4): 355-362 DOI |
21 | Liu P, Zhang L, Zhang H, Jiao H, Wu Y (2013) Detection and molecular variability of Apple stem grooving virus in Shaanxi, China. Journal Phytopathology. 161: 445-449 DOI |
22 | Feng C, Wang R, Li J, Wang B, Yin Z, Cui Z, Li B, Bi W, Zhang Z, Li M, Wang Q (2013) Production of pathogen-free horticultural crops by cryotherapy of in vitro-Grown shoot tips. Methods in Molecular Biology. 994: 463-482 DOI |
23 | Menzel W, Jelkmann W, Maiss E (2002) Detection of four apple viruses by multiplex RT-PCR assays with complification of plant mRNA as internal control. Journal Virological Methods 99: 81-92 DOI |
24 | Murashige & Skoog (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiology plant 15: 473-497 DOI |
25 | Paduch-Cichal E, Kryczynski S (1987) A low temperature therapy and meristem-tip culture for eliminating four viroids from infected plants. Journal Phytopathology 118: 341-346 DOI |
26 | El-Dougdoug KA, Osman ME, Abdelkader HS, Dawoud RA, Elbaz RM (2010) Elimination of Hop stunt viroid (HSVd) from infected peach and pear plants. Australian Journal of Basic and Applied Science 4:54-60 |
27 | Faccioli VC, Marani F (1998) Virus elimination by meristem tip culture and tip micrografting. In plant virus disease control. Hadidi A et al. (eds). American Phytopathological Society. 346-380 |
28 | Hansen AJ (1989) Antiviral chemicals for plant disease control. Critical Reviews in Plant Science 8:45-88 DOI |
29 | Gambino G, Perrone I , Gribaudo I (2008) A rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants. Phytochemical Analysis. 19:520-525 DOI |
30 | Hansen A, Lane W (1985) Elimination of apple chlorotic leaf spot virus from apple shoot cultures by ribavirin. Plant Disease 69:134-135 |
31 | Hollings M (1965) Disease control through virus-free stock. Annual Review of Phytopathology. 3: 367-396 DOI |
32 | Hu G, Dong Y, Zhang Z, Fan X, Ren F, Zhou J (2015) Virus elimination from in vitro apple by thermotherapy combined with chemotherapy. Plant Cell Tissue Culture 121:435-443 DOI |
33 | Joung YH, Jeon JH, Choi KH, Kim HS, Yi YS, Joung H (1997) An Effective Detection of Potato Virus Y Using RT-PCR Technique. Korean Journal Plant Pathology. 13: 219-224 |
34 | Kim JS, Lee SH, Choi HS, Kim MK, Kwak HR, Nam M, Kim JS, Choi GS, Cho JD, Cho IS, Chung BN (2011) Occurrence of virus diseases on major crops in 2010. Research in Plant Disease. 17: 334-341 DOI |
![]() |