• Journal of Life Science
• /
• v.19 no.2
• /
• pp.241-248
• /
• 2009

In order to investigate the effects of synbiotics on change of chemical composition and fermentation characteristics of total mixed ration (TMR), eight TMRs fermented by synbiotics composing the anaerobic microbes (bacteria, yeast, mold) were alloted to the experimental treatments. Treatments were composed of untreated synbiotics(US), bacterial synbiotics (BS), yeast synbiotics (YS), mold synbiotics (MS), bacterial and mold synbiotics (BMS), yeast and mold synbiotics (YMS), bacterial and yeast synbiotics (BYS), and bacterial, yeast and mold synbiotics (BYMS). After 7 days of anaerobic fermentation, fermented-TMRs were exposed to air during 1, 3, 5, 7, 14 and 21 days. One hundred forty four (8 treatments ${\times}$ 6 exposing days ${\times}$ 3 replications) fermented- TMRs were manufactured by vinyl bag sized of 43 cm by 58 cm. The results obtained were as follows. Moisture contents of the fermented TMRs anaerobically ranged from 41% to 45%, and was similar to those of basal TMRs. As results of anaerobic fermentation, the concentration of crude protein was decreased by 11.7% to 14.8% in the untreated sample, while was rather increased by 11% when the TMR was fermented with BMYS. And also BMYS treatment showed decreases by 32% for crude fiber, 15.5% for NDF and 26.1% for ADF. Internal temperature of fermented-TMRs was highest at 7 day of exposing in the air. The pH of fermented-TMR juice was significant difference betweentreatments after 7 day of exposing in air, and that of BMS was highest at 14 day after exposing in air (P<0.05). Acid buffering capacity was increased in proportion to the exposing day of TMR, and peaked at 7 or 14 days after exposing. Ammonia concentration of fermented-TMRs was highest at 5 day after exposing in the air. Individual volatile fatty acid of fermented-TMR juice was very low level in all treatments. Although BMYS treatment to TMR inclined to increase in crude protein and decrease in fibers, but there were no positive effects on the fermentation characteristics after exposing in the air by supplementation of anaerobic synbiotics to TMR.

• Proceedings of the Korean Operations and Management Science Society Conference
• /
• 2003.11a
• /
• pp.205-208
• /
• 2003

대학이 신입생을 선발하는 기준은 해당전공을 공부하는데 적합한 지원자를 선발하는 것이며, 해당모집단위에 많이 지원하게 하여 신입생 충원을 용이하게 하는 것 등이다. 대학은 입학전형을 거쳐서 신입생을 선발하게 되며, 선발기준으로 전형요소를 활용하게 된다. 전형요소 활용방법은 신입생 선발에 영향을 주기 때문에 전략적으로 중요하다. 2005학년도 신입생 선발에는 고등학교 7차 교육과정을 이수한 지원자들이 지원하게 되므로 전형요소에 있어서 변화가 있다. 대학입학수학능력 시험의 체제는 수험생들이 영역이나 과목을 선택하여 응시하는 방향으로 변화한다. 즉, 수리 영역을 가형 및 나형으로 응시하고, 하나의 탐구영역을 응시하되 사회탐구영역 및 과학탐구영역은 4과목 이내에서 선택하여 응시한다. 또한 수학능력시험의 성적표는 각 영역별 및 과목별 표준점수, 백분위점수 및 등급을 표시하여 통지한다. 본 연구는 변화된 수학능력시험의 체제와 고등학교 교육과정을 어떠한 방법으로 반영하여 학생을 선발하는 것이 바람직한가에 대한 전략을 도출하는 틀을 제시하고, 각 전형요소 활용의 대안과 문제점을 도출하고자 한다. 2005 수능시험 결과는 표준점수로 통지하기 때문에 만점 개념을 적용하기 어렵고, 표준점수를 전형요소로 활용할 때 전형총점 개념을 도입하기 어렵다. 또한 복수영역 및 과목의 선택에서 유리함과 불리함이 나타나게 된다. 과거의 수능시험결과를 분석하여 전형총점개념 도입의 방법과 불리함을 보정하여 주는 방법을 제시하고, 신입생을 선발하는 목적에 적합한 전형요소 결정전략을 도출하고자 한다.2; Learning Decisions, 2001) 연구모형을 설정하고 이를 근거로 실증연구를 수행 중에 있다.7.2 $e^{0.101}$x/, y = 70.01 $e^{0.030}$x/, 반감기는 12.0, 6.86, 23.0 일이고 폐장, 간장, 신장의 회복기간(x)별 크롬농도(y)의 소실속도 상관계수 (노출농도 0.50 mg/㎥군의 경우)는 y = 1808 $e^{0.004}$93x/, y = 12.02 $e^{0.029}$7x/, y = 67.61 $e^{0.029}$2x/ 반감기는 140.6, 23.3, 23.7 일로 평가되었다. 4. 고찰 : 실험동물의 전혈, 혈청, 뇨에서의 크롬농도와 시험물질 노출농도는 밀접한 상관을 가졌으나 농도에 정비례하지는 않았다. 뇨 중 흡수된 크롬의 경우 회복기간 초기 (12시간 내)에 대부분 배설이 일어나는 것으로 나타났다. 폐장이 간장, 신장 등 다른 장기에 비해 높은 축적량을 보였으며 축적된 크롬농도가 높을수록 크롬의 소실속도는 현저히 저하하는 경향을 보였다. 노출농도가 높을수록 각 장기조직 내 크롬의 소실속도 (clearance)는 크게 감소경향이 있었으며 이는 체내 과부하시 자정작용이 감소하는 것으로 판단되었다. 본 연구 결과 SD rat를 이용 반복흡입노출의 경우 생체의 무유해영향농도 (NOAEL)는 0.2mg/㎥이하이며 발암물질을 감안하여 안전계수를 100으로 할 경우 사람에 대한 NOAEL은 0.002mg/㎥이하로 판단되

• Korean Journal of Environmental Biology
• /
• v.31 no.3
• /
• pp.204-212
• /
• 2013

This study analyzed the occurrence of tunic softness, survival rate, metabolic rate and histopathologic changes arising from the effect of hypoxic environment in order to find the causes of occurrences of tunic softness, which manifests as the key phenomenon of mass mortality of Halocynthia roretzi. Regarding the survival of H. roretzi with reduction in dissolved oxygen, all the entities died on the 4th day of exposure to the dissolved oxygen concentration of $2mg\;L^{-1}$ while 50% mortality was observed on the 5th day of exposure to the dissolved oxygen concentration of $3mg\;L^{-1}$. Therefore the 5 days-$LC_{50}$ was found to be $3.55mg\;L^{-1}$ (1.86~$4.96mg\;L^{-1}$). However, occurrence of tunic softness was not observed during the period of exposure to low oxygen concentration. The oxygen consumption rate significantly decreases at the dissolved oxygen concentration of less than $5mg\;L^{-1}$ in comparison to the control group. Therefore, it is presumed that H. roretzi controls the respiration rate for prescribed period of time when exposed to hypoxic environment. Regarding the histopathologic changes in the gill, digestive gland and cyst of H. roretzi due to hypoxic environment, necrosis of epithelial layer, in filtration of blood cells, and condensation of nucleus that compose each of the organs were observed. Regarding morphological changes, the decrease in volume with shrinking of the tunic, discoloration of the internal organs and necrosis of gill and hepatopancreas were observed.

• Korean Journal of Ecology and Environment
• /
• v.50 no.3
• /
• pp.305-313
• /
• 2017

We investigate that the impact of freshwater organism exposed to the salinity environment by the frequent rainfall following climate change. To evaluate the stress response following salinity exposure, we assessed the survival rate, molting success rate, the developmental period and mouthpart deformities in Chironomus riparius. In addition, we measured the molecular responses of biomarker gene, gene expression of heat shock protein 70 (HSP70) in C. riparius exposed to salinity after 96 hour. The C. riparius survival rates were showed on time dependent manner and not observed survival organisms above 15 psu at day 4. The pupation and emergence of C. riparius were not seen above 15 psu, and the molting success rate was less than 20% at 10 psu. The developmental retardation of C. riparius was well observed in the pupation and emergence period and was delayed by 4 days at 10 psu compared to the control and 5 psu. The mouthpart deformities after salinity exposure at 96 or 72 hour were observed at 10 psu and 15 psu. The expression of C. riparius HSP70 level was significantly increased exposure to 5 psu and 10 psu. Thus, salinity has been caused to be various ecotoxicological and molecular stress responses on freshwater organisms similar to harmful substances such as EDCs and so on.

• Korean Journal of Environmental Biology
• /
• v.32 no.1
• /
• pp.49-57
• /
• 2014

Orb-web spiders can be considered as an effective bio-indicative animal to monitor the ecological air pollution of certain habitat since they have limited shifting ability during their life spans. In this study we revealed the fine structural characteristics of booklung in the orb-web spider Nephila clavata, and its bio-indicative significance in the monitoring environmental asbestos exposure. The high resolution scanning electron microscopy results suggest that the cuticular spikes near the atrium could be act as an effective filtering structure for dust materials. Furthermore, inner cuticular spikes which protruded across the air sacs, have the functions not only sustaining appropriate respiratory volume of each air sac but also help to enhance respiratory capacity by maximizing the gross surface area for gas exchange. Interestingly, in spite of short exposure, the asbestose fibrils were effectively captured on the surface of the atrial cuticular spikes. Furthermore, histologic damages were observed due to penetration of asbestos fibrils through air sacs and fixed on the hemolymph space. In addition, although accumulated mass of hemocytes were observed near the fibrils, there were no asbestos bodies or coagulated hemocytes were found on the surface of the fibrils, This could be mainly due to the short exposure period towards asbestos. Briefly, these results indicate the spider's booklung could be valuable tool in the detection ecological air pollutants.

• Journal of agricultural medicine and community health
• /
• v.48 no.1
• /
• pp.41-49
• /
• 2023

Objectives: To obtain audiologic basic data to diagnose the noise induced hearing loss of workers in fisheries. Methods: The charts of the referred fishermen with noise induced hearing loss from November 2022 to February 2023 at a general hospital were retrospectively reviewed. Pure tone audiometry, speech audiometry, auditory brainstem response test and auditory steady state response test were conducted. Results: All of them were men over 60 years of age, and the average duration of exposure to noise was 38.9 ± 10.8 years, and the average symptom duration of hearing loss was 13.4 ± 4.3 years. Although the hearing thresholds in the high frequencies were higher than thresholds in the low frequencies, the audiogram showed a down-sloping pattern without rebound at 8 kHz. 10.5% of the cases had thresholds greater than 75 dB in high frequencies, but 57.9% had thresholds greater than 40 dB in low frequencies. Other hearing test results of fishermen were similar to those of general noise-induced hearing loss. Conclusions: Although the fishermen were exposed to noise for a long time, they recognized hearing loss late. The hearing threshold in lower frequencies of the fishermen was higher than expected. Further studies will be needed to analyze the audiologic characteristics of noise-induced hearing loss of the fishermen after confirming noise exposure by conducting a survey on the working environment, such as the noise level and working hours.

• Proceedings of the Korean Institute of Industrial Safety Conference
• /
• 1998.05a
• /
• pp.71-76
• /
• 1998

고온플랜트 설비 고온요소의 재질열화 정도를 평가하기 위해서 사용되는 방법 중 입계부식법은 시험절차가 간단하고 실제 플랜트에서의 적용이 용이하여 안전 및 수명진단시에 많이 이용되어 왔다. 본 논문에서는 오랜 기간 고온에서 사용된 고온$\cdot$고압플랜트의 실기 요소에 입계부식법을 적용하여 재질의 고온에서의 장시간 노출에 의한 인성저하로 발생되는 잔여 수명을 평가한 결과를 제시하였다. (중략)

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 2003.02a
• /
• pp.284-291
• /
• 2003

농산물은 수확된 후부터 계속해서 호흡, 증발, 대사작용을 계속한다. 이런 제반 활동에 기인된 품질손실을 수송과정에서 회복시킬 수는 없으며 단지 농산물은 자체내의 대사작용에 의해서만 에너지를 공급받아 품질을 유지해야 한다. 수확 직후부터 농산물은 세포의 부패로 인해 품질이 저하되기 시작하며 저장기간이 줄어들게 된다 결국 농산물은 수송하고 처리하는 동안의 온도변화, 수분감소, 가스발생 빈도, 물리적 손상, 위생상의 문제 등에 노출된다. (중략)

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 2003.02a
• /
• pp.69-76
• /
• 2003

청과류는 수송과 취급기간 중에 충격, 마찰, 눌림, 타박 등에 지속적으로 노출된다. 그러므로 농산물의 품질을 저하시키는 물리적인 손상을 최소화하기 위해 최선의 처리와 수송방법을 선택할 필요가 있다. 충격흡수 시스템이 갖추어진 대형 냉동 트레일러에 체리, 복숭아, 배, 토마토를 수송했을 때 3.5Hz에서 수직가속도가 가장 크게 나타났고 9-25Hz에서는 이보다는 약간 감소했다. (중략)

• Proceedings of the KSCN Conference
• /
• 2004.05a
• /
• pp.406.1-406
• /
• 2004

유방암은 선진국형 질병으로 미국의 경우 가장 흔한 암으로 보고되고 있으며, 우리나라의 경우 과거에는 유방암 발생률이 낮았으나, 생활방식이 서구화되고 영양상태가 좋아지면서 점차 증가하고 있다. 유방암이 증가하는 이유는 명확히 밝혀지지는 않았지만, 서구화 되어가는 생활 패턴으로 초경 연령이 빨라지고, 폐경연령이 늦어지는 등 유방암 발생에 중요한 역할을 하는 여성호르몬에 노출되는 기간이 길면 길수록 유방암 발생이 증가하는 것으로 알려져 있다. 가족 중에 유방암이 있거나 출산하지 않을 경우 등에서도 유방암의 발생률이 다소 높다고 한다.(중략)