Browse > Article
http://dx.doi.org/10.11614/KSL.2017.50.3.305

Gene Expression of Chironomus riparius Heat Shock Protein 70 and Developmental Retardation Exposure to Salinity  

Kim, Won-Seok (Department of Fisheries and Ocean Science, Chonnam National University)
Im, Byeong-Hyeon (Department of Fisheries and Ocean Science, Chonnam National University)
Hong, Cheol (Department of Fisheries and Ocean Science, Chonnam National University)
Choi, Seung-Won (Department of Fisheries and Ocean Science, Chonnam National University)
Park, Kiyun (Department of Fisheries and Ocean Science, Chonnam National University)
Kwak, Ihn-Sil (Department of Fisheries and Ocean Science, Chonnam National University)
Publication Information
Korean Journal of Ecology and Environment / v.50, no.3, 2017 , pp. 305-313 More about this Journal
Abstract
We investigate that the impact of freshwater organism exposed to the salinity environment by the frequent rainfall following climate change. To evaluate the stress response following salinity exposure, we assessed the survival rate, molting success rate, the developmental period and mouthpart deformities in Chironomus riparius. In addition, we measured the molecular responses of biomarker gene, gene expression of heat shock protein 70 (HSP70) in C. riparius exposed to salinity after 96 hour. The C. riparius survival rates were showed on time dependent manner and not observed survival organisms above 15 psu at day 4. The pupation and emergence of C. riparius were not seen above 15 psu, and the molting success rate was less than 20% at 10 psu. The developmental retardation of C. riparius was well observed in the pupation and emergence period and was delayed by 4 days at 10 psu compared to the control and 5 psu. The mouthpart deformities after salinity exposure at 96 or 72 hour were observed at 10 psu and 15 psu. The expression of C. riparius HSP70 level was significantly increased exposure to 5 psu and 10 psu. Thus, salinity has been caused to be various ecotoxicological and molecular stress responses on freshwater organisms similar to harmful substances such as EDCs and so on.
Keywords
Chironomus riparius; salinity; developmental retardation; heat shock protein 70; mouthpart deformity;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Deinlein, U., A.B. Stephan, T. Horie, W. Luo, G. Xu and J.I. Schroeder. 2014. Plant salt-tolerance mechanisms. Trends in Plant Science 19(6): 371-379.   DOI
2 Dias, V., C. Vasseur and J.M. Bonzom. 2008. Exposure of Chironomus riparius larvae to uranium: effects on survival, development time, growth and mouthpart deformities. Chemosphere 71(3): 574-581.   DOI
3 Dickman, M., I. Brindle and M. Benson. 1992. Evidence of teratogens in sediments of the Niagara river watershed as reflected by Chironomid (Diptera: Chironomidae) deformities. Journal of Great Lakes Research 18(3): 467-480.   DOI
4 Dong, Y., S. Dong and X. Meng. 2008. Effects of thermal and osmotic stress on growth, osmoregulation and Hsp70 in sea cucumber (Apostichopus japonicus Selenka). Aquaculture 276(1): 179-186.   DOI
5 Elendt, B.P. and W.R. Bias. 1990. Trace nutrient deficiency in Daphnia magna cultured in standard medium for toxicity testing; effects of the optimization of culture conditions on life history parameters of Daphnia magna. Water Research 24: 1157-1167.   DOI
6 Farooq, M., M. Hussain, A. Wakeel and K.H.M. Siddique. 2015. Salt stress in maize: effects, resistance mechanisms and management. Agronomy for Sustainable Development 35(2): 461-481.   DOI
7 Fleury, E., J. Moal, V. Boulo, J.Y. Daniel, D. Mazurais, A. Henaut, C. Corporeau, P. Boudry, P. Favrel and A. Huvet. 2010. Microarray-based identification of gonad transcripts differentially expressed between lines of Pacific oyster selected to be resistant or susceptible to summer mortality. Marine Biotechnology 12(3): 326-339.   DOI
8 Hassell, K.L., B.J. Kefford and D. Nugegoda. 2006. Sub-lethal and chronic salinity tolerances of three freshwater insects: Cloeon sp. and Centroptilum sp. (Ephemeroptera: Baetidae) and Chironomus sp. (Diptera: Chironomidae). Journal of Experimental Biology 209(20): 4024-4032.   DOI
9 Hussain, M., H.W. Park, M. Farooq, K. Jabran and D.J. Lee. 2013. Morphological and physiological basis of salt resistance in different rice genotypes. International Journal of Agriculture & Biology 15: 113-118.
10 Heye, K., D. Becker, C.L. Eversloh, V. Durmaz, T.A. Ternes, M. Oetken and J. Oehlmann. 2016. Effects of carbamazepine and two of its metabolites on the non-biting midge Chironomus riparius in a sediment full life cycle toxicity test. Water Research 98: 19-27.   DOI
11 Ibrahim, H., R. Kheir, S. Helmi, J. Lewis and M. Crane. 1998. Effects of organophosphorus, carbamate, pyrethroid and organochlorine pesticides and a heavy metal on survival and cholinesterase activity of Chironomus riparius Meigen. Bulletin of Environmental Contamination and Toxicology 60(3): 448-455.   DOI
12 Lorenz, M.W. and G. Gade. 2009. Hormonal regulation of energy metabolism in insects as a driving force for performance. Integrative and Comparative Biology 49(4): 380- 392.   DOI
13 Kim, W.S., K. Park, C. Nikapitiya and I.S. Kwak. 2015. The molecular biomarker genes expression of rearing species Chironomus riparious and field species Chironomus plumosus exposure to heavy metals. Korean Journal of Ecology and Environment 48: 86-94.   DOI
14 Kim, W.S., K. Park, J.K. Kim and I.S. Kwak. 2016. Expression of the heat shock protein 70 gene and external developmental traits of two Bivalvia species, Crassostrea gigas and Mytilus galloprovincialis, under aquaculture environments. Korean Journal of Ecology and Environment 49(1): 22-30.   DOI
15 Lee, S.M., S.B. Lee, C.H. Park and J. Choi. 2006. Expression of heat shock protein and hemoglobin genes in Chironomus tentans (Diptera, chironomidae) larvae exposed to various environmental pollutants: a potential biomarker of freshwater monitoring. Chemosphere 65(6): 1074-1081.   DOI
16 OECD. 2004. Test no. 219: sediment-water chironomid toxicity using spiked water. OECD Guidelines for the Testing of Chemicals (Paris, France).
17 Meregalli, G., L. Pluymers and F. Ollevier. 2001. Induction of mouthpart deformities in Chironomus riparius larvaed exposed to 4-n-nonylphenol. Environmental Pollution 111(2): 241-246.   DOI
18 Mouguelar, V.S. and G. Coux. 2012. Amphibian oocytes release heat shock protein A during spawning: evidence for a role in fertilization. Biology of Reproduction 87(2): 33.   DOI
19 Nikapitiya, C., W.S. Kim, K. Park and I.S. Kwak. 2014. Identification of potential markers and sensitive tissues for low or high salinity stress in an intertidal mud crab (Macrophthalmus japonicus). Fish & Shellfish Immunology 41(2): 407-416.   DOI
20 Nybom, I., S. Abel, K. Maenpaa and J. Akkanen. 2016. Effects of activated carbon amended sediment on biological responses in Chironomus riparius multi-generation testing. Journal of Hazardous Materials 318: 388-395.   DOI
21 Oraez, I., G. Morcillo and J.L. Martinez-Guitarte. 2016. Ultraviolet filters differentially impact the expression of key endocrine and stress genes in embryos and larvae of Chironomus riparius. Science of the Total Environment 557: 240-247.
22 Paital, B. and G.B.N. Chainy. 2010. Antioxidant defecses and oxidative stress parameters in tissues of mud crab (Scylla serrata) with reference to changing salinity. Comparative Biochemistry and Physiology, Part C 151: 142-151.
23 Park, K. and I.S. Kwak. 2008. Characterization of heat shock protein 40 and 90 in Chironomus riparius larvae: effects of di (2-ethylhexyl) phthalate exposure on gene expressions and mouthpart deformities. Chemosphere 74(1): 89- 95.   DOI
24 Romano, N. and C. Zeng. 2012. Osmoregulation in decapod crustaceans: implications to aquaculture productivity, methods for potential improvement and interaction with elevated ammonia exposure. Aquaculture 334: 12-23.
25 Park, K. and I.S. Kwak. 2013. Expression of stress response HSP70 gene in Asian paddle crabs, Charybdis japonica, exposure to endocrine disrupting chemicals, bisphenol A (BPA) and 4-nonylphenol (NP). Ocean Science Journal 48(2): 207-214.   DOI
26 Park, K. and I.S. Kwak. 2014. The effect of temperature gradients on endocrine signaling and antioxidant gene expression during Chironomus riparius development. Science of the Total Environment 470: 1003-1011.
27 Park, K., H.W. Bang, J. Park and I.S. Kwak. 2009. Ecotoxicological multilevel-evolution of the effects of fenbendazole exposure to Chironomus riparius larvae. Chemosphere 77(3): 359-367.   DOI
28 Park, K., J. Park and I.S. Kwak. 2010. Biological and molecular responses of Chironomus riparius (Diptera, Chironomidae) to herbicide 2,4-D (2,4-dichlorophenoxyacetic acid). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 151(4): 439-446.   DOI
29 Ricketts, C.D., W.R. Bates and S.D. Reid. 2015. The effects of acute waterborne exposure to sublethal concentrations of molybdenum on the stress response in rainbow trout, Oncorhynchus mykiss. Plos One 10(1): e0115334.   DOI
30 Somero, G.N. 2002. Thermal physiology and vertical zonation of intertidal animals: optima, limits and costs of living. Integrative and Comparative Biology 42(4): 780-789.   DOI
31 Tamura, K., G. Stecher, D. Peterson, A. Filipski and S. Kumar. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725- 2729.   DOI
32 Yang, Y.N., H. Ye, H. Huang, S. Li, X. Liu, X. Zeng and J. Gong. 2013. Expression of Hsp70 in the mud crab, Scylla paramamosain in reponse to bacterial, osmotic, and thermal stress. Cell Stress and Chaperones 18(4): 475-482.   DOI
33 Velki, M., D. Kodrík, J. Večeřa, B.K. Hackenberger and R. Socha. 2011. Oxidative stress elicited by insecticides: a role for the adipokinetic hormone. General and Comparative Endocrinology 172(1): 77-84.   DOI
34 Wang, Q.I., S.S. Yu, C.X. Qin, S.I. Dong and Y.W. Dong. 2014. Combined effects of acute thermal and hypo-osmotic stresses on osmolality and hsp70, hsp90 and sod expression in the sea cucumber Apostichopus japonicus Selenka. Aquaculture International 22(3): 1149-1161.   DOI
35 Wu, R., Y. Sun, L. Lei and S. Xie. 2008. Molecular identification and expression of heat shock cognate 70 (HSC70) in the Pacific white shrimp Litopenaeus vannamei. Molecular Biology 42(2): 234-242.   DOI
36 Zhang, J.L. and H. Shi. 2013. Physiological and molecular mechanisms of plant salt tolerance. Photosynthesis Research 115(1): 1-22.   DOI
37 Zhou, J., W.N. Wang, W.Y. He, Y. Zheng, L. Wang, Y. Xin, Y. Liu and A.L. Wang. 2010. Expression of HSP60 and HSP70 in white shrimp, Litopenaeus vannamei in response to bacterial challenge. Journal of Invertebrate Pathology 130(3): 170-178.
38 Chaney, M.L. and A.Y. Gracey. 2011. Mass mortality in Pacific oysters in associated with a specific gene expression signature. Molecular Ecology 20(14): 2942-2945.   DOI
39 Anderson, R.L. 1977. Chironomidae toxicity tests - biological background and procedures. In: Buikema, A.L., Cairns, J. (Eds.), Aquatic Invertebrate Bioassays. American Society for Testing and Materials (ASTM). 209pp.
40 Bervoets, L., C. Wils and R. Verheyen. 1996. Tolerance of Chironomus riparius larvae (Diptera: Chironomidae) to salinity. Bulletin of environmental contamination and Toxicology 57: 829-835.   DOI
41 Cui, Z., Y. Liu, W. Luan, Q. Li, D. Wu and S. Wang. 2010. Molecular cloning and characterization of a heat shock protein 70 gene in swimming crab (Portunus trituberculatus). Fish & Shellfish Immunology 28(1): 56-64.   DOI
42 Davies, S.A., P. Cabrero, G. Overend, L. Aitchison, S. Sebastian, S. Terhzaz and J.A. Dow. 2014. Cell signaling mechanisms for insect stress tolerance. Journal of Experimental Biology 217(1): 119-128.   DOI