• Korean Journal of Breeding Science
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• v.41 no.1
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• pp.25-31
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• 2009

This study was conducted in order to breed Japonica salt tolerant rice and select salinity tolerant lines by establishing reliable and practical method of screening voluminous materials. Salinized nutrient solution by adding NaCl was effective compared with the salinized nutrient solution by adding 0.3%, 0.6% and 0.7% of diluted sea water. There was no different visual score of salinity injury between salinization using tap water and distilled water. Seedling salinity tolerant lines between region and order by year were showed very stable and reproducible results, 3~4.2 of visual score at Gyehwado-substation and International Rice Research Institute (IRRI, Philippines) in 2002, 2003 and 2004. Heading date of 6 selected seedling salt tolerant lines showed a range of 16. Aug.~21. Aug. and delayed 2~6 days than that of Donjinbyeo. Percentage of ripened grain and yield of milled rice in 6 lines was lower, 52.2~14.7% and 50~5%, respectively than those of Donjinbyeo.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.1
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• pp.1-8
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• 1992

This study was conducted to obtain the basic information on salt-tolerance of rice cultivars in relations with growth retardation and cation content. As the salt-level increased, less retardation in plant height and dry weight were shown in salt-tolerant than salt-susceptible rice cultivars. Salt tolerant cultivars showed lower N $a^{+}$ and higher $K^{+}$ content and lower N $a^{+}$ / $K^{+}$ ratio at each salt-levels than salt-susceptible ones, while there were no significant differences in $Ca^{++}$ and $Mg^{++}$ content. At the tillering stage, the plant height and dry weight of the salt-treated plots were significantly correlated with N $a^{+}$ and $K^{+}$ content and N $a^{+}$ / $K^{+}$ ratio, implying that N $a^{+}$ and $K^{+}$ content could be an indicator of salt-tolerance of a rice cultivar. There were no tiller-depending differences in cation content in all cultivars. N $a^{+}$ content and N $a^{+}$ / $K^{+}$ ratio in leaves were lower at the top and higher at the bottom. In three $F_1$ hybrids between salt tolerant parent Pokkali and three salt susceptible parents, plant height, dry weight, $K^{+}$ content and N $a^{+}$ / $K^{+}$ ratio were similar to those of Pokkali parent, while N $a^{+}$ content was intermediate of the parents. So, it seemed that salt tolerance is dominant over salt susceptibility. In $F_2$ of Pokkali/wx817 cross, genetic segregation of plant height, dry weight, $K^{+}$ content and N $a^{+}$ / $K^{+}$ ratio varied continuously and was biased onto Pokkali side, while that of N $a^{+}$ content showed normal distribution with intermediate mode. Broad-sense heritability of the characters ranged from 0. 604 to 0. 811. Genotypic and phenotypic correlation coefficients among them were relatively high.fficients among them were relatively high.h.h.

• Applied Biological Chemistry
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• v.50 no.1
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• pp.82-85
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• 2007

내염성 조건에서 phytase를 생성하는 효모 균주를 개발하기 위하여 phytase를 생산하는 효모인 S. cerevisiae CY 균주와 내염성 효모인 Z. rouxii Y-80 균주 사이의 전기적 세포융합을 실시하였다. S. cerevisiae CY 균주의 Z. rouxii Y-80 균주의 배양된 세포를 1.0%의 ${\beta}$-mercaptoethanol로 전처리하고 Tunicase로 세포벽을 분해시킨 후 원형질체를 얻었다. 각 균주의 원형질체는 electrofusion 완충액에 1:1의 비율로 현탁한 후 AC 13V에서 30초간 처리하고 고전압(DC 500 V/100 ${\mu}sec$)을 가하여 전기적 세포융합을 실시하였다. 선별된 융합균주는 NaCl이 10% 첨가된 배지에서 친주들과 비교한 결과 Z. rouxii Y-80 균주보다는 생육면에서 25% 이상 증가하였으며, phytase 활성도는 친주인 S. cerevisiae CY 균주 대비 53%의 생성도를 확인하였다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.3
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• pp.270-274
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• 1997

This experiment was conducted to evaluate the combining ability of japonica rices for improving the salinity tolerance at seedling stage. Nine varieties used in partial diallel analysis included three varieties of each tolerant, moderately tolerant and susceptible one. Twelve-day-old seedlings were grown in saline solution, initially at an EC of 6 dS /m for four days, followed by an EC of 12 dS /m for 20 days. The agronomic characters at seedling stage such as plant height, dry shoot weight and dry root weight were measured to analyze for combining ability of the parents. General combining ability(GCA) and specific combining ability(SCA) effects were highly significant for all tested parameters. However, mean squares for GCA were about five times larger than that for SCA suggesting the preponderance of additive gene action. Among tolerant varieties, Gaori and Namyang 7 were good combiner for improving the salinity tolerance at seedling stage in GCA as well as SCA.

• The Microorganisms and Industry
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• v.14 no.3
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• pp.31-32
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• 1988

염농도는 미생물 생육에 중요한 환경요인의 하나로서 대부분의 미생물들은 염농도 1% 이내를 생육 최적조건으로 하지만 자연계에는 염농도 1% 이상에서 포화염농도까지 생육이 가능한 것들도 많다. 미생물 중에는 생육 최적염농도보다 높은 환경에 처하면 여러가지 적응기작에 의해 고염분 농도에 적응하면서 생육할 수 있는 내염성 미생물과 고염농도 환경을 생육 최적조건으로 하는 호염성 미생물로 대별할 수 있다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.4
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• pp.406-415
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• 2020

Maize (Zea mays L.) is one of the most valuable agricultural crops and is grown under a wide spectrum of environmental conditions. However, maize is moderately sensitive to salt stress, and soil salinity is a serious threat to its production worldwide. In this study, we used ethyl methane sulfonate (EMS) to generate salt-tolerant silage maize mutants. We screened salt-tolerant lines from 203 M₃ mutant populations by evaluating the morphological phenotype after salt stress treatment and selected the 140ES91 line. The 140ES91 mutant showed improved plant growth as well as higher proline content and leaf photosynthetic capacity compared with those of wild-type plants under salt stress conditions. Using whole-genome re-sequencing analysis, 1,103 single nucleotide polymorphisms and 71 insertions or deletions were identified as common variants between KS140 and 140ES91 in comparison with the reference genome B73. Furthermore, the expression patterns of three genes, which are involved in salt stress responses, were increased in the 140ES91 mutant under salt stress. Taken together, the mutant line identified in our study could be used as an improved breeding material for transferring salt tolerance traits in maize varieties.

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.108-109
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• 2000

• Proceedings of the Korean Society of Crop Science Conference
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• 1991.10a
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• pp.56-57
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• 1991

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.1-8
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• 2011

Recently the increasing of vinyl and green houses and development of reclaimed land including Saemangeum induced the need for breeding salt-tolerant crops which can survive and grow in high salinity soil. So we try to develop salt-tolerant transgenic chrysanthemum (Dendranthema grandiflorum.) lines by using anti-porter gene TANHX and HVNHX. Through marker selection and plant regeneration step, we could get 284 putative transgenic chrysanthemum lines. On selected putative transgenic plants, 40 candidates were used for genetic analysis and 30 lines could be made up of target size band on PCR, so about 75% of marker selected lines were decided as real transgenic lines. Selected 284 transgenic lines were also used for salt-tolerance test as a range of NaCl 0.2 ~ 1.2% (300 mM). As a result of salt-tolerance test, 15 selected transgenic lines could live and grow on the continuous supply of 0.8% (200 mM) NaCl solution and another 7 lines were could survive under 1.2% (300 mM) NaCl solution. This salt-tolerant transgenic lines under salt stress also lead a cell alternation especially a guard cell. A stressed guard cell be swelled and grow larger in proportion to NaCl concentration. TTC test for cell viability on transgenic chrysanthemum lines pointed out that more strong salt-tolerant lines can be live more than another under same salt stress. The numerical value of strong salt-tolerant 7 transgenic lines were 0.206 ~ 0.331 under 1.2% NaCl stress, and then it's value is more larger than middle salinity lines' 0.114 ~ 0.193 and non-transgenic's 0.046. And the proline contents as indicated stress compound also pointed out that HVNHX introduced salt-tolerant transgenic lines were less stressed than other under same salt stress. The contents of strong salt-tolerant transgenic lines were 2.255 ~ 2.638 mg/kg and it is much higher than that of middle salinity lines' 1.496 ~ 2.125.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.559-562
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• 2000

A halotolerant and extracellular pretense producing yeast was isolated from traditional Meju and identified as a strain of Hansenular sp. S-9 by investigation of its microbiological characteristics. The optimum pH, temperature and NaCl concentration for growth of Hansenular sp. S-9 were pH 7.5, $30^{\circ}C$ and 0.5M, respectively. The protease production from Hansenular sp. S-9 was maximized when it was grown on BD medium containing 1.0% beef extract, 1.0% glucose and 0.5M NaCl for 72 h at $30^{\circ}C$.